Being considered that Work-Family benefits can possibly improve work-family balance, quality of life, and women resources utilization, the public opinion has been over the debate in modern society. However, previous researches generally focused on application of work-family benefits program, while individual-level factors received relatively less attention in researches, So, I conducted this study to focus on employee’s perceived usefulness of work-family benefits. This research intended to analyze how perceived organizational culture-as one of antecedent factors-influences employee’s perceived usefulness of work-family benefits. It was hypothesized perceived usefulness of work-family benefits has a positive effect on organizational effectiveness such as job satisfaction, organizational commitment and turnover intention. It also intended to prove that the moderating effects of social support in relationship between employee’s perceived usefulness of work-family benefits and organizational effectiveness. In order to prove the hypothesis, a questionnaire survey was conducted with 195 employees. The collected data were analyzed by the SPSS, multiple regression analysis was used for statistical analysis. The results of this study are as follows. The findings showed that adhocracy culture positively affected employee’s perceived usefulness of work-family benefits. In addition, employee’s perceived usefulness of work-family benefits was positively related to job satisfaction, but organizational commitment and turnover intention were not statistically significant. In verification of social support as a moderating variable, supervisor support had the moderating effect between employee’s perceived usefulness of work-family benefits and organizational effectiveness engagement by strengthening the relationship. However, moderating effect of coworker support was not shown in the relationship between employee’s perceived usefulness of Work-Family Benefits and organizational effectiveness.