Organic Trace Mineral Supplementation Research Articles

PSVIII-A-1 Effect of Source of Trace Mineral and Bismuth Subsalicylate on Growth, Ruminal H2s, and Trace Mineral Status of Growing Heifers

Abstract This study evaluated the effects of providing inorganic, chelated, and inorganic plus injectable trace mineral supplementation strategies with or without the inclusion of bismuth subsalicylate (BSS) on dry matter intake (DMI), water intake, ruminal hydrogen sulfide (H2S) concentration, and trace mineral status of growing beef heifers provided high sulfate (5,055 ± 228 mg/L) water. This study consisted of an 84-d feeding period with a 2×2 + 1 factorial treatment arrangement conducted using 2 blocks. Beef heifers (n = 15/block; 367 ± 33 kg BW) in each block were stratified according to their initial liver copper concentration. Mineral treatments included inorganic [added CuSO4 (7.76 mg/kg), ZnSO4 (22.92 mg/kg), MnSO4 (15.28 mg/kg)], chelated (100% of the Cu-methionine (7.68 mg/kg ), Zn-methionine (22.89 mg/kg), and Mn-methionine (15.27 mg/kg), and injectable trace mineral (15 mg/mL Cu, 10 mg/mL Mn, 60 mg/mL Zn) provided in combination with the inorganic mineral treatment. The BSS treatments were 0.0 (CON) or 0.2% (BSS) on a DM basis. Feed and water intake (weekly), ruminal H2S concentration (d 42 and 84), and liver (pre-study, d 42 and 84) and serum trace mineral concentrations (d 1, 28, 56, and 84) were evaluated. Initial liver trace mineral concentration was used as a covariate. Dry matter intake tended to be greater (mineral × BSS, P = 0.05) for heifers provided chelated minerals and fed CON, compared with BSS, and tended to be less for heifers fed inorganic and CON than BSS. Heifers fed chelated minerals drank 6.1 L/d more (P < 0.05) water than those provided inorganic minerals. Injectable trace-mineral provision did not affect DMI or water intake. Ruminal H2S was not affected by mineral type or BSS (P ≥ 0.33). The inclusion of BSS reduced (P < 0.01) liver Cu concentration from 60.5 to 31.3 ppm. Heifers provided the injectable minerals had greater (P < 0.01) liver Cu concentration than the inorganic without BSS treatment. Serum copper was not affected by BSS or mineral treatment (P ≥ 0.44). The liver concentration of Se was reduced (P < 0.01) by the inclusion of BSS. The serum Se concentration was not affected by mineral type, BSS, time, or any two- or three-way interactions (P ≥ 0.24). Bismuth subsalicylate did not affect ruminal H2S, and negatively affected liver Cu concentration. The use of organic trace mineral supplementation strategies did not affect the trace mineral status of beef heifers drinking high sulfate water, but use of an injectable trace mineral supplement increased liver Cu.

The Effect of Organic Trace Mineral Supplementation in the Form of Proteinates on Performance and Sustainability Parameters in Laying Hens: A Meta-Analysis.

The effect of supplementing organic trace minerals (OTM), in the form of mineral proteinates (Bioplex® Cu, Fe, Mn, and Zn, Alltech Inc., Nicholasville, KY, USA), in the diets of laying hens was examined using Comprehensive Meta-Analysis (CMA) statistical software. The impact on production performance, egg quality traits, and sustainability parameters related to the carbon footprint of egg production was assessed. Data were obtained from 32 global studies, comprising 107 dietary assessments of 30,992 laying hens. Overall pooled effect size (raw mean difference) of production performance when dietary organic trace minerals were supplemented either in basal diets, partial replacement of inorganic trace minerals (ITM), or total replacement of ITM, indicated that use of Bioplex minerals resulted in 2.07% higher hen-day production (HDP), whilst feed conversion ratio (FCR) was lower by 51.28 g feed/kg egg and 22.82 g feed/dozen eggs, respectively. For egg quality traits, daily egg mass was 0.50 g/hen/day higher and egg weight was 0.48 g per egg greater when Bioplex minerals were incorporated in the diet. The mean difference in egg loss was -0.62%. Eggshell thickness was greater by 0.01 mm, and a higher eggshell strength of 0.14 kgf was observed. Eggshell weight was heavier by 0.20 g, eggshell percentage was higher by 0.15%, and Haugh unit was 1 point higher (0.89). We also carried out a meta-regression of the effects of the study factors (location, year of study, hen breed/strain, age of hens, number of hens, and study duration) on the overall pooled effect size of the production performance and egg quality traits in response to supplementary OTM inclusion, and it indicated that certain factors had a significant (p < 0.05) impact on the results. Finally, a life cycle assessment (LCA) model was selected to evaluate the impact of feeding organic trace mineral proteinates on the carbon footprint (feed and total emission intensities) of the egg production using the data generated from the meta-analysis. Results showed that the inclusion of OTM proteinates resulted in an average drop in feed and total emission intensities per kg eggs of 2.40% and 2.50%, respectively, for a low-global-warming-potential (GWP) diet and a drop of 2.40% and 2.48% for feed and total emissions, respectively, based on high-GWP diet. Based on the overall results, the inclusion of organic trace mineral proteinates in layer diets can benefit production performance and egg quality traits while contributing to a lower carbon footprint.

Dietary Supplementation with Chromium DL-Methionine Enhances Growth Performance of African Catfish (Clarias gariepinus).

Sustainable aqua feeds have become an urgent necessity for future-oriented aquaculture sector development, and especially mineral supply could be limited when diets are being prepared with low amounts of animal-based sources. Since knowledge about the efficiency of organic trace mineral supplementation in different species of fish is limited, the effects of chromium DL-methionine in African catfish nutrition were evaluated. Four commercially based diets with increasing chromium DL-methionine supplementation (0, 0.2, 0.4, and 0.6 mg Cr kg-1) in the form of Availa-Cr 1000 were fed to African catfish (Clarias gariepinus B., 1822) in quadruplicate groups for 84 days. Growth performance parameters (final body weight, feed conversion ratio, specific growth rate, daily feed intake, protein efficiency ratio, and protein retention efficiency), biometric indices (mortality, hepatosomatic index, spleen somatic index, and hematocrit), and mineral retention efficiency were assessed at the end of the feeding trial. The specific growth rate was significantly increased in fish-fed diets with 0.2 mg Cr kg-1 and 0.4 mg Cr kg-1 supplementation in comparison with control and based on the second-degree polynomial regression analysis; supplementation with 0.33 mg Cr kg-1 was optimal in commercially based diets for African catfish. Chromium retention efficiency was reduced with increasing supplementation levels; however, the chromium content of the whole body was comparable to literature. The results suggest that organic chromium supplementation is a viable and safe supplement for diets to increase the growth performance of African catfish.

The effect of dietary supplementation of organic trace minerals on performance, mineral retention, lymphoid organs and antibody titres of broilers

Complete replacement of inorganic trace minerals (ITM) with proteinated organic trace minerals (PTM) at equal or lower inclusion rates was evaluated. One thousand and eight, one-d-old male chicks were divided into 24 pens containing 42 chicks, and randomly allocated to one of the following: T1: control group with ITM supplied at the standard commercial level in Ecuador; T2: PTM at 100% T1; T3: PTM at 66% TI; and T4: PTM at 33% T1. The 42-d experiment employed a three-phase feeding programme (1-14, 15-28 and 29-42 d). Restricted feeding was used to prevent the development of ascites associated with high altitude. At 21 and 42 d of age, Cu, Mn and Zn retention were measured in tibial bone, and Fe in whole blood. Lymphoid organ weights were at 21 and 42 d of age. Antibody titres were measured by ELISA at 42 d of age. Weight gain, feed consumption, feed conversion and mortality were similar among treatments, although were below breed expectations due to feed restriction practices. Blood Fe was not affected by treatment (P&lt;0.05). At both 21 and 42 d of age, minerals in tibia differed (P&lt;0.01), with Mn and Zn concentrations being significantly higher in all PTM groups, compared to ITM control at 42 d. However, at 21 d, Zn was higher for the ITM-fed birds. No differences in lymphoid organ (bursa, thymus, and spleen) weights were observed, or for Gumboro (infectious bursal disease), infections bronchitis virus and reovirus antibody titres. For Newcastle disease virus, T4 birds had significantly lower antibody titres compared to other treatment groups. In conclusion, replacement of inorganic minerals with a proteinated form organic minerals at lower inclusion rates had no negative impact on performance, lymphoid organ weight or antibody titres in broilers raised under commercial conditions of high altitude and restricted feeding.

Effects of Chromium-L-Methionine in Combination with a Zinc Amino Acid Complex or Selenomethionine on Growth Performance, Intestinal Morphology, and Antioxidative Enzymes in Red Tilapia Oreochromis spp.

Simple SummaryThe purpose of this study was to develop diet optimization for the growth and health of fish under intensive aquaculture systems, with a focus on the farming of Nile tilapia and red tilapia in Thailand. Diets and proper nutrition are more important than ever in raising healthy, antibiotic-free fish. Organic trace minerals have been used to promote the growth and health status of fish in aquafeeds. In this investigation, we studied the effects of chromium-L-methionine, in combination with a zinc amino acid complexor in combination with selenomethionine, on the growth performance, feed utilization, and immune responses of red tilapia. The results for the combination of chromium-L-methionine with a zinc amino acid complex showed beneficial effects for red tilapia in terms of growth performance, feed utilization, and intestinal morphology. The results for the combination of chromium-L-methionine with selenomethionine demonstrated that the levels of antioxidative enzymes, especially glutathione, could be increased to provide an innate immune response in red tilapia.To consider diet optimization for the growth and health of fish under intensive aquaculture systems, with a focus on the farming of Nile tilapia and red tilapia in Thailand, we conducted an experiment based on a completely randomized design (CRD), with three treatments and four replicates. Three diets, supplemented with different trace minerals, were applied to selected groups of fish: (a) a control diet, without organic trace minerals supplementation; (b) a T1 diet of chromium-L-methionine at 500 ppb, in combination with a zinc amino acid complex at 60 parts per million (ppm); and (c) a T2 diet of chromium-L-methionine at 500 ppb in combination with selenomethionine at 300 ppb. Red tilapia with an initial mean weight of 190 ± 12 g/fish were randomly distributed into cages of 2 × 2 × 2.5 m in a freshwater pond (12 cages in total), with 34 fish per cage and a density of 17 fish/m3. During the 8 week feeding trial, the fish were fed 3–4% of their body weight twice a day. The fish were weighed, then blood samples were collected to study their immune responses. The intestines were collected, measured, and analyzed at the end of the feeding trial. The results showed that the red tilapia that were fed with diets of chromium-L-methionine in combination with a zinc amino acid complex in the T1 treatment had significantly (p < 0.05) higher final weights, weight gains, average daily gains (ADGs), and better feed conversion ratios (FCRs), compared with fish that were fed with the control diet without organic trace minerals and with fish that were fed with the T2 diet (p < 0.05). The midgut and hindgut villus heights of the group fed with chromium-L-methionine in combination with a zinc amino acid complex in the T1 treatment were significantly higher than those of the other groups (p < 0.05). The levels of the antioxidative enzyme superoxide dismutase (SOD) and lysozyme activity were not significantly different from those of fish that were fed with the control diet (p > 0.05), whereas the glutathione level tended to increase (p < 0.1) in fish that were fed with chromium-L-methionine in combination with selenomethionine in the T2 treatment. Therefore, we concluded that chromium-L-methionine in combination with a zinc amino acid complex or selenomethionine clearly enhanced red tilapia’s growth performance and feed utilization through the promotion of antioxidative enzyme activity and immune response.

Effects of organic trace mineral supplementation on the prevalence of digital dermatitis in beef feedlot cattle

The aim of the study was to evaluate the effects of organic trace mineral (OTM) supplementation (Availa Plus, Zinpro Corporation) on the prevalence of digital dermatitis (DD) and growth performance in feedlot beef cattle. A blinded, randomized, controlled field study in a commercial beef cattle feedlot was performed for 11 mo. The study was conducted in 2 phases: the adaptation phase comprised the initial 60 d of feeding the control (CON) and OTM supplements, and the postadaptation phase continued until cattle were sent to slaughter. A total of 1,120 beef heifers were enrolled in the study distributed over 8 pens. The CON and OTM supplements were fed in their respective TMR. Regression analysis at the animal level indicated that during the postadaptation phase, there was a significantly lower rate of increase in DD lesions in the OTM group compared with the CON group. However, the pen-level analysis revealed that there were no statistically significant differences in DD lesions between the CON and OTM groups during the postadaptation phase. The analysis of hot carcass weight, centered around the mean, revealed that the OTM supplementation was significantly associated with an increase of 9.7 kg (2.9–16.5 95% CI) above the centered hot carcass weight per head at slaughter compared with cattle fed the CON diet. Centering around the mean was performed by subtracting the mean hot carcass weight from each hot carcass weight value in the data set. The OTM supplementation may help prevent DD and increase performance of beef feedlot cattle.

Low Level of Dietary Organic Trace Minerals Improved Egg Quality and Modulated the Status of Eggshell Gland and Intestinal Microflora of Laying Hens During the Late Production Stage.

This study aimed to investigate the effects of dietary organic trace minerals on egg quality and intestinal microflora of laying hens during the late production stage. In total, 1,080 Jinghong-1 laying hens aged 57 weeks were randomly assigned to five treatment groups: CON, basal diet containing about 6, 29, 49, and 308 mg·kg−1 of Cu, Mn, Zn, and Fe; IT100, basal diet supplemented with 10, 80, 80, and 60 mg·kg−1 of Cu, Mn, Zn, and Fe (each as inorganic sulfates), respectively; OT20, basal diet supplemented with 2, 16, 16, and 12 mg·kg−1 of Cu, Mn, Zn, and Fe (each as organic trace minerals chelated with lysine and methionine in the ratio of 2:1 amino acid: organic trace minerals), respectively; OT30, basal diet supplemented with 3, 24, 24, and 18 mg·kg−1 of organic Cu, Mn, Zn, and Fe, respectively; and OT50, basal diet supplemented with 5, 40, 40, and 30 mg·kg−1 of organic Cu, Mn, Zn, and Fe, respectively. Overall, OT20, OT30, and OT50 had equal or higher potential to promote Cu, Mn, Zn, and Fe deposition in egg yolks compared with IT100. In addition, OT50 enhanced the eggshell breaking strength and the antioxidant status of the eggshell gland. Cecal microbiota, including Barnesiellaceae and Clostridia, were significantly decreased in IT100- and OT50-treated hens compared with the CON group. Clostridia UCG-014 was negatively correlated with eggshell weight and OCX-32. In conclusion, reduced supplementation of organic trace minerals can improve the eggshell quality and trace mineral deposition, possibly by modulating genes involved in the eggshell formation in the eggshell gland and by controling of the potentially harmful bacteria Barnesiellaceae and Clostridiales in the cecum. Inorganic trace minerals may be effectively replaced by low level of complex organic trace minerals in laying hens during the late production stage.

Effect of trace mineral source on biochemical and hematological parameters, digestibility, and performance in growing lambs.

To study the efficacy of organic trace mineral supplementation on blood parameters, digestibility, and growth as compared to inorganic sources, 18 Zandi male lambs (with initial body weight, 28.5 ±1.4 kg and 110±5 days old) were divided into three groups of six animals in each in a completely randomized design. Lambs in the control group were fed basal diet containing 63 kg/100 kg of concentrate mixture, 22 kg/100 kg of alfalfa hay, and 15 kg/100 kg wheat straw. Animals in the experimental groups were additionally supplemented with trace minerals supplied by sulfates or a diet in which 25.7 ppm Zn, 14.3 ppm Mn, 8.9 ppm Cu from mineral-amino acid complex, and 0.86 ppm Co from Co glucoheptonate replaced with similar amounts of Zn, Mn, Cu, and Co from sulfates. All lambs were kept in individual pens with cemented floor and provision of individual feeding and watering. Lambs fed with either organic or inorganic trace mineral supplement showed higher dry matter intake and growth rate and better feed conversion efficiency (P<0.05) as compared to the control group. Blood glucose, urea nitrogen, cholesterol, and hepatic enzymes were similar among the treatments. Triglycerides (P<0.01) concentration was lower for mineral-supplemented groups. Blood vitamin B12 concentration increased with mineral supplementation and was higher for the lambs fed with organic source of trace elements as compared with those fed with inorganic mineral and the control diet (P=0.04). The results of this study showed that feeding organic trace elements improves growth performance of finishing lambs but did not affect nutrient digestibility and blood parameters.

Strategies of inorganic and organic trace mineral supplementation in gestating hyperprolific sow diets: effects on the offspring performance and fetal programming.

The aim of the present study was to evaluate the effect of trace mineral nutrition on sow performance, mineral content, and intestinal gene expression of neonate piglets when inorganic mineral sources (ITM) were partially replaced by their organic mineral (OTM) counterparts. At 35 d postmating, under commercial conditions, a total of 240 hyperprolific multiparous sows were allocated into three experimental diets: 1) ITM: with Zn, Cu, and Mn at 80, 15, and 60 mg/kg, respectively; 2) partial replacement trace mineral source (Replace): with a 30 % replacement of ITM by OTM, resulting in ITM + OTM supplementation of Zn (56 + 24 mg/kg), Cu (10.5 + 4.5 mg/kg), and Mn (42 + 18 mg/kg); and 3) Reduce and replace mineral source (R&R): reducing a 50% of the ITM source of Zn (40 + 24 mg/kg), Cu (7.5 + 4.5 mg/kg), and Mn (30 + 18 mg/kg). At farrowing, 40 piglets were selected, based on birth weight (light: <800 g, and average: >1,200 g), for sampling. Since the present study aimed to reflect results under commercial conditions, it was difficult to get an equal parity number between the experimental diets. Overall, no differences between experimental diets on sow reproductive performance were observed. Light piglets had a lower mineral content (P < 0.05) and a downregulation of several genes (P < 0.10) involved in physiological functions compared with their average littermates. Neonate piglets born from Replace sows had an upregulation of genes involved in functions like immunity and gut barrier, compared with those born from ITM sows (P < 0.10), particularly in light piglets. In conclusion, the partial replacement of ITM by their OTM counterparts represents an alternative to the totally inorganic supplementation with improvements on neonate piglet gene expression, particularly in the smallest piglets of the litter. The lower trace mineral storage together with the greater downregulation of gut health genes exposed the immaturity and vulnerability of small piglets.

Effect of replacing inorganic trace minerals at lower organic levels on growth performance, blood parameters, antioxidant status, immune indexes, and fecal mineral excretion in weaned piglets.

Organic trace minerals (OTMs) have the potential to replace inorganic trace minerals (ITMs), but the degree to which the dietary levels can be reduced is not well defined. This study aimed to investigate the effect of replacing of ITMs with lower levels of OTMs on growth performance, blood parameters, antioxidant status, and immune indexes in weaned piglets. The experiment was conducted in a subtropical city in Guangdong Province in South China (subtropical climate) from July to September 2018. A total of 600 pigs with an average initial BW of 8.90kg were allotted by gender and weight to 5 treatments with 6 replicate pens per treatment. Experimental treatments: (A) Control group (a basal diet with iron, copper, manganese, and zinc from sulfates and sodium selenite providing commercially utilized levels in China of 150, 25, 40, 150, and 0.5mg/kg, respectively). (B) 1/2 ITM group (inorganic trace minerals providing 1/2 control group levels). (C) 1/2 OTM group (1/2 control group trace mineral levels with manganese, iron, zinc, and selenium from Sel-Plex® and Cu from Bioplex®). (D) 1/3 ITM group (1/3 control group trace mineral levels from inorganic forms). (E) 1/3 OTM group (1/3 control group trace mineral levels from organic forms). The results suggest no significant effects of trace mineral sources or levels, on average daily gain (ADG) and average daily feed intake (ADFI) among different treatments during the entire experiment. The level of zinc in serum was significantly decreased in the 1/3 ITM group. The 1/3 OTM group had a significantly higher (P < 0.05) immunoglobulin G (IgG) level in serum. Fecal mineral excretion decreased significantly (P < 0.05) when decreased dietary levels of trace minerals were included at 1/2 and 1/3 levels regardless of sources. Fecal concentrations of zinc excretion were lower (P < 0.05) with 1/2 OTM supplementation than 1/2 ITMs. The present study shows that replacing high doses of ITMs with low concentrations (1/3) of OTMs does not adversely affect the growth performance of piglets. At low levels, total replacement of ITMs with OTMs improved IgG and reduced fecal excretion of copper, zinc, iron, and manganese, thereby mitigating environmental pollution.

Effect of Dietary Supplementation of Organic Trace Minerals at Reduced Concentrations on Performance, Bone Mineralization, and Antioxidant Variables in Broiler Chicken Reared in Two Different Seasons in a Tropical Region

Two experiments were conducted to study the performance, antioxidant activity, and bone mineral variables in broilers fed organic trace minerals (OTM) at lowered concentrations in the diet. In experiment 1, a total of 1500 day-old broiler male chicks were randomly distributed into six groups with 10 replicates of 25 chicks each and housed in floor pens (1.90 × 1.22m). One group was fed the maize-soybean meal-based control diet, supplemented with inorganic trace mineral (ITM) premix containing Mn, Zn, Fe, Cu, Se, and Cr at 50, 45, 40, 7.5, 0.30, and 0.25mg/kg, respectively. The remaining groups of chicks were fed the diets, where the ITM premix was replaced with OTM mix so as to provide the respective minerals at graded levels in the diet (100, 75, 50, 40, and 30% of the control diet). Similarly, in experiment II, a total of 1350 day-old male broiler chicks were divided at random into six groups with 9 replicates of 25 chicks each and fed the maize-soybean meal-based control diet having ITM at levels similar to those of experiment I or the test diets, where the ITM was replaced with OTM so as to provide the minerals at 100, 80, 60, 40, and 20% of the ITM-based control diet. Each diet was fed ad libitum from 0 to 42days of age. The body weight gain, feed intake, and feed efficiency were not affected by supplementing OTM at 30 and 20% in experiments I and II, respectively. Slaughter variables, activities of glutathione peroxidase and super oxide dismutase, and lipid peroxidation and ferric reducing activity in serum were not affected by supplementing OTM at the lowest level of 20% in the diet. Similarly, bone-breaking strength, ash, and Mn contents were not affected by supplementing OTM at 20% of the mineral concentration in control diet containing ITM. Deposition of Ca, P, Cu, and Fe in tibia ash increased with increased concentration of OTM in the diet. However, Zn content in tibia ash reduced with reduction in OTM level in the diet. Based on the results, it is concluded that trace mineral supplementation in organic form even at 20% of the concentration recommended for ITM may be sufficient in maize-soybean meal-based diet to support the optimum growth, bone ash, bone strength, and serum antioxidant status in commercial broilers.

The Effects of Organic Trace Mineral Supplementation on Heritage Rhode Island Red Breeding Stock

The Effects of Organic Trace Mineral Supplementation on Heritage Rhode Island Red Breeding Stock

Comparative effects of inorganic and organic trace minerals (Zn, Se and Cr) supplementation on expression of ChTLR2b gene in broilers

Poultry is the fastest growing livestock sector in the developing countries. The global poultry sector is expected to continue to grow, as demand for poultry meat is driven by growing needs, population, rising incomes and urbanization. The present experiment was conducted to investigate the effect of inorganic and organic trace minerals supplementation on expression of ChTLR2b gene in broilers. A total of 216 broilers randomly divided into twelve groups and each group consisting of 18 broilers in 3 replicates. T1 group was kept as control. T2, T3 and T4 group was supplemented with zinc (40 mg/kg of feed) inorganic, organic and 50% organic form respectively. T5, T6 and T7 groups was supplemented with selenium (0.3 mg/kg of feed) from inorganic, organic and 50%organic form respectively. T8, T9 and T10 groups was supplemented with chromium (2 mg/kg of feed) from inorganic, organic form and 50% organic form respectively. T11 and T12 group was supplemented with combination of all 3 minerals from inorganic and organic form respectively. RT-PCR expression analysis of ChTLR2b gene in spleen revealed that maximum up regulation (3.8413 fold) was found in T3 group (supplemented with organic Zn @ 40 mg/Kg of feed), followed by T4 (3.5325 fold) whereas in bursa of fabricius the maximum up regulation (2.8921 fold) was found in T6 group followed by T12 (2.5310 fold ) as compared to control group. Up-regulation of gene expression ChTLR2b in bursa of fabricius and spleen indicates beneficial effect of organic trace minerals in potentiation of immune system in broilers.

Effects of dietary organic trace mineral mixture levels on survival, growth performance, body composition and antioxidant capacity of juvenile turbot ( Scophthalmus maximus )

A 70-day feeding trial was conducted to investigate effects of dietary organic trace mineral (OTM) mixture levels on survival, growth performance, body composition and antioxidant capacity of juvenile turbot (Scophthalmus maximus). The commercial diet with 0.03% inorganic trace mineral (ITM) premix was used as the control, and other four experimental isonitrogenous (50% crude protein) and isolipidic (12% crude lipid) diets were formulated to contain 0.0375%, 0.075%, 0.1125% and 0.15% OTM mixture respectively. Results showed that there were no significant differences in survival among dietary treatments. However, the growth was not significantly different between the control and OTM supplementation diets. Especially, turbot fed the diet with 0.075% OTM had significantly higher specific growth rate than 0.15%, 0.1125% and 0.0375% OTM treatments (p < .05). Moisture, crude protein, crude lipid and ash of the whole fish body showed no significant differences among dietary treatments. Activities of superoxide dismutase in serum of turbot fed diets with 0.1125% and 0.15% OTM were significantly higher than 0.0375% OTM treatment (p < .05). Activities of total antioxidant capacity and catalase in serum were the highest, while the malondialdehyde content was the lowest when the turbot were fed the diet with 0.075% OTM. In summary, results of the present study suggested that the supplementation of 0.0375% or more OTM in diets could obtain similar performance to the commercial diet with ITM premix, and the optimal amount of OTM supplementation in diets of turbot was approximately 0.075% on basis of growth performance and antioxidant capacity.

Different dietary trace mineral sources for broiler breeders and their progenies

The objective of this study was to evaluate the effect of different trace mineral supplementation sources in the diet of broiler breeders on their performance and on their progenies. In total, 128 Cobb 500 broiler breeders were distributed according to a completely randomized experimental design in 2 experimental treatments. The control group was fed a diet supplemented with inorganic trace minerals (ITM), while the other group was fed a diet supplemented with reduced levels of trace minerals in the organic form. Eggs were collected when breeders were 35, 47, and 53 wk old. Their progeny (450 hatchlings) were divided according to trace mineral supplementation source from the maternal diet, creating 2 treatments with 16 replicates of 15 birds each. Organic trace mineral (OTM) supplementation improved broiler breeder performance, as shown by higher egg production and better eggshell quality of OTM-fed hens compared with those fed ITM. Egg fertility and hatchability were not influenced by the treatments. As to progeny performance, higher weight gain, and consequently, better feed conversion ratio, were obtained in the 41-day-old progenies of OTM-fed breeders, independently of hen age. Maternal diet trace mineral source did not affect broiler carcass, breast meat, or leg yields. The results of the present study show that supplementing broiler breeder diets with organic trace mineral sources enhances the performance of breeders and their progenies.

Low-dose of organic trace minerals reduced fecal mineral excretion without compromising performance of laying hens.

ObjectiveThe objective of this study was to investigate the effects of low doses of organic trace minerals (iron, copper, manganese, and zinc) on productive performance, egg quality, yolk and tissue mineral retention, and fecal mineral excretion of laying hens during the late laying period.MethodsA total of 405 healthy hens (HY-Line White, 50-week-old) were randomly divided into 3 treatments, with 9 replicates per treatment and 15 birds per replicate. The dietary treatments included feeding a basal diet + inorganic trace minerals at commercial levels (CON), a basal diet + inorganic trace minerals at 1/3 commercial levels (ITM), and a basal diet + proteinated trace minerals at 1/3 commercial levels (TRT). The trial lasted for 56 days.ResultsCompared to CON, ITM decreased (p<0.05) egg production, daily egg mass, albumen height, eggshell strength, yolk Fe concentration, serum alkaline phosphatase activity and total protein, and increased (p<0.05) egg loss and feed to egg ratio. Whereas with productive performance, egg quality, yolk mineral retention, and serum indices there were no differences (p>0.05) between CON and TRT. The concentrations of Fe and Mn in the tissue and tibia were changed notably in ITM relative to CON and TRT. Both ITM and TRT reduced (p< 0.05) fecal mineral excretion compared to CON.ConclusionThese results indicate that dietary supplementation of low-dose organic trace minerals reduced fecal mineral excretion without negatively impacting hen performance and egg quality.

Evaluation of the prevalence of digital dermatitis and the effects on performance in beef feedlot cattle under organic trace mineral supplementation.

Bovine digital dermatitis (DD) is a contagious and multifactorial disease that leads to painful, ulcerative lesions of the skin near the heel-horn border of the foot, most commonly in dairy cattle. With regard to beef cattle, the pathogenesis and etiology of DD has not been widely reported or studied over the past several decades. A longitudinal field trial in a commercial feedlot was conducted to compare prevalence and effects of DD in beef steers provided a diet supplemented with a novel formulation of inorganic and organic trace mineral sources (OTM diet) compared to a diet provided with similar levels of trace minerals solely from inorganic sources (CON diet). A secondary objective was to evaluate the prevalence of DD and the potential effects on growth performance and carcass yield and quality. One thousand seventy-seven steers were assigned to 1 of the 2 treatment groups (CON diet or OTM diet) based on location of their home pens which were situated in 1 of 2 barns. All pens in the B barn (group B) were assigned to the OTM diet, and all pens in the A barn (group A) were assigned to the CON diet. The study was conducted in 2 phases: adaptation phase (AP) comprising the initial 60 d of feeding CON and OTM diets and postadaptation phase (PAP) which lasted until cattle were sent to harvest. In the AP, pens in group B had a greater proportion of steers (54.03%) with DD lesions compared to pens in group A (26.72%). During the PAP, the relative risk of observing an increased DD prevalence was significantly ( < 0.05) higher in CON group compared to OTM group. Growth performance, final live weight, and hot carcass weight were negatively impacted when steers were observed to have active DD lesions (M2 lesions) compared to steers with no M2 lesions over the study period. For ADG, a calculated loss per steer of 0.08 kg/d from type I (no M2 lesions) to type II (one M2 lesion; SE = 0.028; = 0.003) and loss of 0.14 kg/d from type I to type III (multiple M2 lesions; SE = 0.038; = 0.0003) were observed. A significant BW loss of approximately 10.06 kg (SE = 4.18; = 0.022) and a mean reduction of 5.5 kg per steer in HCW (SE = 2.74; = 0.043) were also found between type I and type II steers.

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Micro minerals (Trace minerals) are required for normal growth and development in broilers. The present study was undertaken to compare the effect of replacing inorganic with organic trace minerals on growth performance, carcass characteristics and chemical composition of broiler thigh meat. A corn soybean based diet supplemented with organic trace minerals (OTM) (x) and an inorganic trace mineral (ITM) (y) was prepared. Four hundred eighty birds were stratified by weight and randomly assigned to 6 dietary treatments with four replicates per treatment (20 birds/replicate pen). The treatments were A) Positive control group diet (x) supplemented for whole period (0-5 wk). B) Fed diet (x) during starter and grower phase (1-4 wk) and diet (y) was offered in finisher phase (5 wk). C) 1st 3 weeks were fed diet (x) and last two weeks were given diet (y). D) First 2 weeks were fed diet (x) while, diet (Y) offered in the last three weeks. In the treatment (E), diet (x) offered during initial phase and nourished with diet (y) during grower and finisher phase while in treatment (F), complete diet (y). The result showed that organic trace mineral supplementation did not affect growth performance in the first and last week of trail but during 2nd, 3rd and 4th weeks organic trace mineral supplementation showed better growth performance than that of inorganic trace mineral supplementation. Chicken fed with organic trace mineral supplemented diets had the better growth performance which differed from that of inorganic supplemented diet groups. Additionally, organic trace minerals supplementation did not affect dressing percentage and giblets weight but shank and keel lengths were improved. While, OTM supplementation did not alter dry matter, ash and moisture content in thigh meat; however, crude protein content was improved in thigh meat. Overall results demonstrated that the quality of broiler chicken meat in high organic trace mineral supplement increased relative to the low supplemented groups. It is concluded that addition of organic trace mineral to feed can improve the growth performance of broiler particularly during growing phase. Key words: Carcass, dry matter, growth, performance, replacing.

Effect of Supplemental Trace Minerals on Hsp-70 mRNA Expression in Commercial Broiler Chicken

ABSTRACTThe effects of supplementing the organic forms of selenium (Se), chromium (Cr), and zinc (Zn) on Hsp-70 mRNA expression and body weight in broiler chickens were evaluated. 200 chicks were equally distributed into stainless steel battery brooders at the rate of 5 birds per pen and reared under heat stress condition up to 42nd day. The chicks were fed with three experimental diets supplemented with organic forms of Se (0.30 mg/kg), Cr (2 mg/kg), and Zn (40 mg/kg) during the starter and finisher phases and a control diet without any supplementation. On the 21st and 42nd day, 20 birds from each period were sacrificed and samples were collected for analysis. Organic Se, Cr, and Zn supplementation significantly (P < 0.05) reduced the expression of Hsp-70 mRNA levels. The Hsp-70 mRNA expression levels were significantly (P < 0.05) different between the tissues studied with spleen having the lowest expression level. Hsp-70 mRNA expression level was not affected by age of the birds. The study concluded that organic trace mineral (oTM) supplementation resulted in low Hsp-70 mRNA expression, indicating reduced heat stress in broilers.

Evaluation of the prevalence of digital dermatitis and the effects on performance in beef feedlot cattle under organic trace mineral supplementation

Evaluation of the prevalence of digital dermatitis and the effects on performance in beef feedlot cattle under organic trace mineral supplementation