Organic Accumulation Research Articles

Determination of heavy metals (Pb, Cr, As, Hg, and Cd) into the body organs of selected fish, water, sediment, and soil samples from Head Punjnad and Head Taunsa, Punjab, Pakistan.

The present study was conducted on Head Punjnad (HP) and Head Taunsa (HT) to evaluate the contamination of Pb, Cr, As, Hg, and Cd in water, soil, sediment, fish as a whole and fish organs. Fish, water, soil and sediment samples were collected from different sites of HT and HP on a monthly basis for 8 months. Heavy metals in water, soil, and sediment were determined by a polarized Zeeman atomic absorption spectrophotometer and in fish and fish organs by an atomic absorption spectrophotometer. Contamination of Cd, Hg, and As was significantly (P<0.05) higher in water of HP as compared to HT, while Cr showed a non-significant (P>0.05) difference at HP and HT. Pb was significantly (P<0.05) higher in water of HT as compared to HP. In the case of soil, Cd, Hg, and Pb were higher at HT as compared to HP, while As and Cr were significantly (P<0.05) higher at HP as compared to HT. In sediment, contamination of Cd, Hg, and As were significantly (P<0.05) higher at HP as compared to HT, while the Cr difference was non-significant (P>0.05) but Pb showed a significantly (P<0.05) higher value at HT than HP. Cd accumulation in different fish species was recorded as R. rita ˃O. niloticus ˃C. marulius ˃S. sarwari ˃C. idella ˃C. catla ˃N. notopterus ˃E. vacha ˃L. rohita ˃C. carpio, respectively. Hg as O. niloticus ˃S. sarwari ˃R. rita ˃C. marulius ˃C. catla ˃N. notopterus ˃E. vacha ˃L. rohita ˃C. carpio ˃C. idella, respectively. As as O. niloticus ˃R. rita ˃S. sarwari ˃C. marulius ˃C. catla ˃C. carpio ˃N. notopterus ˃C. idella ˃E. vacha ˃L. rohita, respectively. Cr accumulation recorded as L. rohita ˃C. idella ˃O. niloticus ˃C. marulius ˃E. vacha ˃R. rita ˃C. catla ˃C. carpio ˃S. sarwari ˃N. notopterus, respectively. Pb accumulation in different fish species was recorded as C. idella ˃C. carpio ˃N. notopterus ˃L. rohita ˃O. niloticus ˃C. marulius ˃R. rita ˃S. sarwari ˃E. vacha ˃C. catla, respectively. Cd accumulation in different organs was recorded as kidney ˃liver ˃gills ˃muscle ˃skin ˃scale. Hg accumulation in different organs was recorded as kidney ˃gills ˃liver ˃skin ˃muscle ˃scale. As accumulation in different organs was recorded as kidney ˃liver ˃gills ˃muscle ˃skin ˃scale. Cr accumulation in different organs was recorded as gills ˃ liver ˃skin ˃muscle ˃kidney ˃scale. Pb accumulation in different organs was recorded as gills˃ kidney˃ skin˃ liver˃ muscle˃ scale.

Genome-wide identification of HSF gene family and their expression analysis in vegetative tissue of young seedlings of hemp under different light treatments

Heat shock transcription factors (HSFs), play a crucial role in plant growth, development and defense processes. The functions of some HSFs have been well studied in response some stresses in model plants. At present, the genome of the medicinal plant Cannabis sativa L. has been fully sequenced. Nevertheless, there is little known about the HSF genes family in Cannabis sativa L, as well as a lack of understanding of its evolutionary history. Herein, 18 HSFs were identified in the cannabis genome and phylogenetically classified into three groups based on the structural characteristics and phylogenetic analysis, including classes A (9 genes), classes B (8 genes), and classes C (1 gene). They were distributed over 9 of 10 chromosomes without chromosome 8. Then, the motif composition and evolutionary relationship of CsHSF proteins are discussed in detail, along with gene structures, cis-acting elements in the promoter, and synchronization analysis. Furthermore, our results also revealed that CsHSF transcription levels varied significantly among tissues, suggesting that the functions of CsHSF may be different. Finally, we found that red and blue light treatments had a significant effect on HSF genes expression and cannabinoids accumulation in vegetative organs of hemp young seedlings, implying the expression of HSF genes may regulate the content of cannabinoids under different light conditions. Although cannabinoid biosynthesis and response to light in vegetative organs of hemp young seedlings may not represent the mature stage of hemp, these results provided an essential theoretical basis to elucidate the regulatory mechanism of HSF in cannabinoid biosynthesis in the future.

Advanced theranostic nanoplatforms for hypericin delivery in the cancer treatment

Biomodified coated-lipid vesicles were obtained using the DPPC lipid (L) and F127 copolymer linked covalently with spermine (SN), biotin (BT), and folic acid (FA), resulting in LF127-SN, LF127-BT, and LF127-FA nanoplatforms. The photosensitizer hypericin (HY) was incorporated into the nanosystem by a thin-film method and characterized by dynamic light scattering, zeta potential, encapsulation efficiency, and transmission electronic microscopy. The results provided a good level of stability for all nanoplatforms for at least 5 days as an aqueous dispersion. The in vitro serum stability showed that the HY-loaded LF127-SN has a lower tendency to form complexes with BSA protein than with its analogs. LF127-SN was the most stable HY formulation, followed by LF127-BT and LF127-FA, confirmed by the association constant (Kd) values: 600 μmol L−1, 1100 μmol L−1, 515 μmol L−1, and 378 μmol L−1 for LF127, LF127 FA, LF127-BT, and LF127-SN, respectively. The photodynamic potential of HY was accessed by cytotoxicity assays using Caco-2, B16-F10, L-929, and HaCat cells. HY-loaded LF127-SN revealed a significant increase in the selectivity compared to other nanoplatforms. HY-loaded in LF127-BT and LF127-SN showed distinct uptake and biodistribution after 2 h of intravenous application. All biomodified coated-lipids showed satisfactory metabolism within 72 h after application, without significant accumulation or residue in any vital organ. These results suggest that incorporating HY-loaded in these nanosystems may be a promising strategy for future applications, even with a small amount of binders to the coating copolymer (0.02% w/v). Furthermore, these results indicate that the LF127-SN showed remarkable superiority compared to other evaluated systems, being the most distinct for future photodynamic therapy and theranostic applications.

Sedimentary Environment and Organic Accumulation of the Ordovician–Silurian Black Shale in Weiyuan, Sichuan Basin, China

The sedimentary environment and organic matter enrichment relationship of the Upper Ordovician Wufeng Formation black shale, Guanyinqiao mudstone, and Lower Silurian Longmaxi Formation black shale in the Sichuan Basin of Weiyuan are analyzed using geochemical methods such as organic carbon, sulfur, major elements, and trace elements. The experimental results illustrate that the upper section of the Wufeng Formation and the lower section of the Longmaxi Formation are organic matter enrichment layers. The presence of P indicates a high productivity level in the Sichuan Basin from the Late Ordovician to the Early Silurian. In addition, indicators such as V/Cr, Ni/Co, and S/C suggest that the Wufeng Formation was deposited under anoxic reductive conditions, that the ice age Guanyinqiao segment was in an oxygen-rich to oxygen-poor environment, and that the Longmaxi Formation was in a sulfidic environment. Mo/TOC indicates that the Wufeng Formation shale was controlled by a restricted basin and that the Guanyinqiao segment and the Longmaxi Formation were in a medium-to-weak retention environment. The weak correlation of TOC with P/Al and Al indicates that the level of primary productivity and terrigenous detritus had a minor effect on the organic matter enrichment of the Wufeng and Longmaxi Formation black shale. Conversely, the positive correlation of TOC with V/Cr and Ni/Co illustrates that the anoxic reductive sedimentary environment is the main factor affecting the organic matter enrichment of the Wufeng and Longmaxi Formation black shale. Based on these studies, the development model of organic-rich shales of the Ordovician–Silurian in Weiyuan, Sichuan Basin is proposed. This paper may provide a reference for shale gas exploration in the Wufeng–Longmaxi Formation and a sedimentary response to the major geological events of Ordovician–Silurian.

A critical re‐analysis of constraints on the timing and rate of Laurentide Ice Sheet recession in the northeastern United States

AbstractWe review geochronological data relating to the timing and rate of Laurentide Ice Sheet recession in the northeastern United States and model ice margin movements in a Bayesian framework using compilations of previously published organic 14C (n = 133) and in situ cosmogenic 10Be (n = 95) ages. We compare the resulting method‐specific chronologies with glacial varve records that serve as independent constraints on the pace of ice recession to: (1) construct a synthesis of deglacial chronology throughout the region; and (2) assess the accuracy of each chronometer for constraining the timing of deglaciation. Near the Last Glacial Maximum terminal moraine zone, 10Be and organic 14C ages disagree by thousands of years and limit determination of the initial recession to a date range of 24–20 ka. We infer that 10Be inherited from pre‐glacial exposure adds 2–6 kyr to many exposure ages near the terminal moraines, whereas macrofossil 14C ages are typically 4–8 kyr too young due to a substantial lag between ice recession and sufficient organic material accumulation for dating in some basins. Age discrepancies between these chronometers decrease with distance from the terminal moraine, due to less 10Be inherited from prior exposure and a reduced lag between ice recession and organic material deposition. 14C and 10Be ages generally agree at locations more than 200 km distal from the terminal moraines and suggest a mostly continuous history of ice recession throughout the region from 18 to 13 ka with a variable pace best documented by varves.

Radiometal-Labeled Photoactivatable Pt(IV) Anticancer Complex for Theranostic Phototherapy.

A novel photoactivatable Pt(IV) diazido anticancer agent, Pt-succ-DFO, bearing a pendant deferoxamine (DFO) siderophore for radiometal chelation, has been synthesized for the study of its in vivo behavior with radionuclide imaging. Pt-succ-DFO complexation of Fe(III) and Ga(III) ions yielded new heterobimetallic complexes that maintain the photoactivation properties and photocytotoxicity of the parent Pt complex in human cancer cell lines. Radiolabeled Pt-succ-DFO-68Ga (t1/2 = 68 min, positron emitter) was readily prepared under mild conditions and was stable in the dark upon incubation with human serum. PET imaging of Pt-succ-DFO-68Ga in healthy mice revealed a promising biodistribution profile with rapid renal excretion and limited organ accumulation, implying that little off-target uptake is expected for this class of agents. Overall, this research provides the first in vivo imaging study of the whole-body distribution of a photoactivatable Pt(IV) azido anticancer complex and illustrates the potential of radionuclide imaging as a tool for the preclinical development of novel light-activated agents.

Timing and tempo of organic carbon burial in the Monterey Formation of the Santa Barbara Basin and relationships with Miocene climate

Understanding the transfer of carbon between Earth's surface reservoirs is necessary for interpreting climate transitions in Earth history and predicting future climate change. Warming associated with the 16.9–14.7 Ma Miocene Climate Optimum and subsequent cooling during the 14.7–13.8 Ma Middle Miocene Climate Transition provide opportunities to study carbon cycle dynamics in the geologically recent past. The Monterey Hypothesis interprets the Middle Miocene Climate Transition cooling as part of a positive feedback in which enhanced organic carbon burial on the eastern Pacific margin drew down atmospheric CO2. This idea has been supported by the correlation of organic-rich deposits in the Monterey Formation in coastal California with the mid-Miocene Monterey Event, a globally-observed positive shift in the δ13C of marine carbonates that may be indicative of elevated burial of δ13C-depleted organic carbon. Here, we use 31 new U-Pb zircon laser ablation inductively coupled plasma mass spectrometry ages and 14 new isotope dilution thermal ionization mass spectrometry ages from volcanic ash beds in the Monterey Formation along the Santa Barbara coast to constrain the timing and tempo of organic carbon mass accumulation in some of the most organic-rich rocks in California. The new age model demonstrates that peaks in organic carbon mass accumulation rate in the Monterey Formation do not coincide with the Monterey Event, and that total organic carbon content in the Miocene Santa Barbara Basin is inversely correlated with sedimentation rate. We propose that changes in organic carbon burial rates in the Monterey Formation were driven by a combination of sea-level change and local tectonically-mediated basin formation, which provided first-order controls on sedimentation rate, and that organic carbon burial in the Monterey Formation is better described as a response to, rather than a driver of, global climate.

The interplay between autophagy and chloroplast vesiculation pathways under dark-induced senescence.

In cellular circumstances where carbohydrates are scarce, plants can use alternative substrates for cellular energetic maintenance. In plants, the main protein reserve is present in the chloroplast, which contains most of the total leaf proteins and represents a rich source of nitrogen and amino acids. Autophagy plays a key role in chloroplast breakdown, a well-recognised symptom of both natural and stress-induced plant senescence. Remarkably, an autophagic-independent route of chloroplast degradation associated with chloroplast vesiculation (CV) gene was previously demonstrated. During extended darkness, CV is highly induced in the absence of autophagy, contributing to the early senescence phenotype of atg mutants. To further investigate the role of CV under dark-induced senescence conditions, mutants with low expression of CV (amircv) and double mutants amircv1xatg5 were characterised. Following darkness treatment, no aberrant phenotypes were observed in amircv single mutants; however, amircv1xatg5 double mutants displayed early senescence and altered dismantling of chloroplast and membrane structures under these conditions. Metabolic characterisation revealed that the functional lack of both CV and autophagy leads to higher impairment of amino acid release and differential organic acid accumulation during starvation conditions. The data obtained are discussed in the context of the role of CV and autophagy, both in terms of cellular metabolism and the regulation of chloroplast degradation.

Transcriptome and Metabolome Reveal Sugar and Organic Acid Accumulation in Rosa roxburghii Fruit.

Sugars and organic acids significantly impact fruit sensory quality, but their accumulation patterns and regulatory mechanisms during the development of Rosa roxburghii fruit are still unclear. We utilized transcriptomics and metabolomics to investigate genes related to sugar and organic acid metabolism in Rosa roxburghii. Metabolomics data revealed that sucrose, glucose and fructose were the primary sugars, whereas citric acid and malic acid were the primary organic acids in Rosa roxburghii fruit. We constructed the metabolic pathways of major sugars and organic acids in Rosa roxburghii and identified five key genes involved in sugar and organic acid synthesis. In addition, we identified a module containing 132 transcription factors that was significantly associated with sucrose, citric acid and malic acid. Based on quantitative polymerase chain reaction (qPCR), we identified 13 transcription factors involved in sugar and organic acid metabolism, including the transcription factor RrANL2 and the sucrose synthase gene RrSUS3. Further yeast one-hybrid and dual luciferase assays showed that RrANL2 could bind to the promoter of RrSUS3 to increase its expression. These results provide new insights into the metabolism of sugars and organic acids in Rosa roxburghii fruit.

Potassium Increases Nitrogen and Potassium Utilization Efficiency and Yield in Foxtail Millet

Field experiments were conducted to investigate the effects of K application on the nitrogen and potassium utilization efficiency and yield of foxtail millet (Setaria italica L.). The experiment was performed with a completely randomized design with two millet cultivars (Jingu 21 and Zhangza 10) and five K2O rates (0, 60, 120, 180, and 240 kg/hm2) in 2020 and 2021. We found that K promoted K and N absorption; significantly increased dry matter (DM), N, and K accumulation in millet organs; caused dry matter accumulation to peak earlier; and increased the DM accumulation rate. In addition, K accumulation preceded that of DM or N. Relative to the crop cycle, most K (61.07%) accumulated at booting, whereas N accumulated mostly (33.86%) during grain filling. N absorption efficiency increased by 31.87%, and the apparent and agronomic utilization rate of K fertilizer remained high, increasing millet yield, which peaked at a K rate of 180 kg/hm2 for both cultivars, by 29.91% and 31.51% in Jingu 21 and Zhangza 10, respectively, relative to untreated controls. Stepwise regression and path analysis showed that the leaf and spike K accumulation, stem N accumulation, and stem DM were the main factors affecting yield, with DM having the greatest direct effect, followed by leaf K accumulation. The K concentration (0.77–3.04%) in Zhangza 10 was higher than that in Jingu 21 (0.69–2.91%) in untreated plants. Under the same K application rate, N and K accumulation and the harvest index were higher for Zhangza 10 than those for Jingu 21, and the nutrient utilization ability was greater for Zhangza 10. The results demonstrated that rational K fertilizer application can increase K concentration and accumulation in leaves, promote N metabolism and accumulation, increase N and K utilization efficiency, and improve DM accumulation and millet yield.

Polysaccharides as Economic and Sustainable Raw Materials for the Preparation of Adsorbents for Water Treatment

Adsorption processes, due to their technical simplicity and cost-effectiveness, have arisen as one of the most well-known, straightforward solutions to water pollution. In this context, polysaccharides, due to their abundance, biodegradability, and biocompatibility, are appealing raw materials for the design of adsorbents. Moreover, some of them, such as chitosan, can be obtained from organic waste products, and their use additionally contributes to solving another concerning problem: organic waste accumulation. Unfortunately, due to their low adsorption capacities and/or physicochemical properties, native polysaccharides are not suitable for this purpose. However, there are alternatives that can overcome these physical or chemical limitations, often taking advantage of the versatility of their polyhydroxylated structure. In this context, this review aims to present an overview of the advances from 2019 onwards in the design of new adsorbents for water treatment from cellulose, alginate, chitosan, and starch, addressing the two main strategies reported in the literature: the preparation of either polysaccharide-based composites or polysaccharide derivatives. It is important to point out that, herein, special emphasis is placed on the relationship between the chemical structure and the efficiency as adsorbents of the analyzed materials, in an attempt to contribute to the rational design of adsorbents obtained from polysaccharides.

Eco-friendly synthesized nanoparticles as antimicrobial agents: an updated review.

Green synthesis of NPs has gained extensive acceptance as they are reliable, eco-friendly, sustainable, and stable. Chemically synthesized NPs cause lung inflammation, heart problems, liver dysfunction, immune suppression, organ accumulation, and altered metabolism, leading to organ-specific toxicity. NPs synthesized from plants and microbes are biologically safe and cost-effective. These microbes and plant sources can consume and accumulate inorganic metal ions from their adjacent niches, thus synthesizing extracellular and intracellular NPs. These inherent characteristics of biological cells to process and modify inorganic metal ions into NPs have helped explore an area of biochemical analysis. Biological entities or their extracts used in NPs include algae, bacteria, fungi, actinomycetes, viruses, yeasts, and plants, with varying capabilities through the bioreduction of metallic NPs. These biosynthesized NPs have a wide range of pharmaceutical applications, such as tissue engineering, detection of pathogens or proteins, antimicrobial agents, anticancer mediators, vehicles for drug delivery, formulations for functional foods, and identification of pathogens, which can contribute to translational research in medical applications. NPs have various applications in the food and drug packaging industry, agriculture, and environmental remediation.

Reproductive Toxicity of Nanoparticles: A Comprehensive Review.

The unique characteristics of nanoparticles (NPs) have captivated scientists in various fields of research. However, their safety profile has not been fully scrutinized. In this regard, the effects of NPs on the reproductive system of animals and humankind have been a matter of concern. In this article, we will review the potential reproductive toxicity of various types of NPs, including carbon nanomaterials, dendrimers, quantum dots, silica, gold, and magnetic nanoparticles, reported in the literature. We also mention some notable cases where NPs have elicited beneficial effects on the reproductive system. This review provides extensive insight into the effects of various NPs on sperm and ovum and the outcomes of their passage through blood-testis and placental barriers and accumulation in the reproductive organs.

The Interplay of Sulfur and Selenium Enabling Variations in Micronutrient Accumulation in Red Spinach.

Aside from its importance in human and animal health, low levels of foliar-applied selenate (SeO4) can be advantageous in the presence of sulfur (S), contributing to improved growth, nutrient uptake, and crop quality. A hydroponic experiment in a growth chamber explored the interactive influence of Se and S on micronutrients and several quality indices, such as soluble sugars, organic acids, and total protein concentrations in spinach (Spinacia oleracea L.). Three levels of S (deprivation, adequate, and excessive) with varying quantities of Se (deficient, moderate, and higher) were examined in combination. Under S starvation and along with S nourishment in plant parts, Se treatments were found to cause noticeable variations in plant biomass and the concentrations of the examined elements and other quality parameters. Both Se levels promoted S accumulation in S-treated plants. Although the Se treatment had the opposite effect in shoots, it had a favorable impact on minerals (apart from Mn) in roots grown under S-limiting conditions. The S and Se relationship highlighted beneficial and/or synergistic effects for Mn and Fe in edible spinach portions. Reducing sugars were synergistically boosted by adequate S and moderate Se levels in roots, while in shoots, they were accumulated under moderate-or-higher Se and excessive S. Furthermore, the concentration of the quantified organic acids under S-deficient conditions was aided by various Se levels. In roots, moderate Se under high S application enhanced both malic acid and citric acid, while in the edible parts, higher Se under both adequate and elevated S levels were found to be advantageous in malic acid accumulation. Moreover, by elevating S levels in plant tissues, total protein concentration increased, whereas both moderate and high Se levels (Se1 and Se2) did not alter total protein accumulation in high S-applied roots and shoots. Our findings show that the high S and medium Se dose together benefit nutrient uptake; additionally, their combinations support soluble sugars and organic acids accumulation, contributing ultimately to the nutritional quality of spinach plants. Moreover, consuming 100 g of fresh red spinach shoot enriched with different Se and S levels can contribute to humans' daily micronutrients intake.

An Introduction to Ayurvedic Methods of Bloodletting

Ayurveda aims to prevent and cure diseases. In Ayurvedic system of medicine, bloodletting procedure is a kind of para-surgery practiced for the treatment of specifically blood borne diseases along with other surgical diseases. Therapeutic bloodletting is indicated in abscess, cellulitis, chronic ulcers resulting from snake bite, filariasis, genital infections, gout, herpes, inflammatory conditions, Skin disorders, tumors, ulcers, etc. While mentioning the five methods of body detoxification, the Ayurvedic texts mentions bloodletting as one among them, so it is one of the important therapies in Panchakarma. Bloodletting is also known as Phlebotomy and considered to be a prime treatment modality among the surgical procedures in Ayurveda. With curative aspects it also acts in preventive and promotive aspects. The vitiated impure blood is removed out from the body by the seven procedures - Venesection (Siravyadhana), scrapping (Pracchanna karma), horn application (Sringa avacharana), gourd application (Alabu avacharana), leech application (Jalauka avacharana), application of cupping pot/glass (Ghatiyantra avacharana), and needle application (Suchivyadhana). Bloodletting is also used to lower the iron levels and prevents its accumulation in various organs. Therapeutic bleed is a holistic treatment for impure blood and it helps to bring the body back into its natural and homoeostatic state.

Biochar-mediated Cd accumulation in rice grains through altering chemical forms, subcellular distribution, and physiological characteristics

Biochar can change the availability and morphology of soil Cd. However, the influence of biochar on Cd chemical form and subcellular fraction in rice is poorly understood, particularly under different irrigation methods. A pot experiment of biochar application combined with two irrigation methods (continuous flooding and intermittent irrigation, CF and II) was conducted. The Cd accumulation, chemical form and subcellular fraction in rice organs and the associated physiological responses were examined. Biochar significantly reduced soil available Cd (30.85–47.26% and 32.35–52.35%) under CF and II but increased the Cd content (30.4–63.88% and 13.03–18.59%) in brown rice. Additionally, the Cd content in shoots/grains under II was higher than that under CF. Biochar elevated the Cd soluble fraction in roots while lowered the cell wall fraction under both irrigation methods, whereas the opposite result was observed in leaves. Biochar increased water-, ethanol-, and NaCl-extractable Cd in roots meanwhile increased ethanol-extractable Cd in leaves under both irrigation methods. Moreover, the total amount of water-, ethanol-, and NaCl-extractable Cd in rice roots was higher under II than under CF. Related hormones and antioxidant enzymes may also be involved in biochar-mediated Cd accumulation in rice grains. Thus, changes in Cd chemical form and subcellular fraction in the root and leaf are the main mechanisms of biochar-induced rice grains Cd accumulation.Graphical

Long-term fertilization promotes soil organic nitrogen accumulation by increasing the abundance of keystone microbial cluster across aggregates

Nitrogen (N) in soil mainly occurs in organic forms, with soil organic nitrogen (SON) playing an essential role in supplying N to crops. However, it is unclear how the content and distribution of SON vary among different aggregate sizes, and how SON content is related to soil microorganisms. In this study, we investigated the associations between SON content and various microbial community characteristics across different aggregate sizes using data from a long-term field fertilization experiment in paddy soils. We also performed functional prediction to explore potential microbial mechanisms underlying SON accumulation. The results showed that SON content decreased as aggregate size decreased, with macroaggregates having the highest SON content and the silt-clay fraction having the lowest. Furthermore, our linear regression and structural equation modeling analysis revealed that keystone microbial clusters (highly interconnected taxa within Firmicutes, Proteobacteria, Chloroflexi, Actinobacteriota, and Ascomycota) were significantly correlated with SON content. Finally, functional prediction analysis suggested that this relationship may be due, at least in part, to the large number of metabolic pathways involved in biosynthesis of N-containing compounds present in keystone microbial clusters. Overall, our study highlights the importance of keystone microbial clusters in influencing the accumulation of SON and contributes to our understanding of soil N biogeochemical cycling.

Transcriptome data-based identification and expression profiling of genes potentially associated with malic acid accumulation in plum (Prunus salicina Lindl.)

Chinese plum (Prunus salicina Lindl.) is an important commercially produced stone fruit that accumulates high amounts of organic acids with numerous health benefits. Organic acids are key components to determine the flavor of any fruit. Here, to identify the potential genes involved in organic acids accumulation in plum, the metabolomic and transcriptomic analysis of plum fruits were performed at four developmental stages (i.e., fruitlet, green, veraison, and mature). The results suggest that malic acid was predominant among the eight organic acids analyzed. RNA-Seq analysis revealed a total of 26,547 unigenes, of which 7,584 representing 28.56% differentially expressed genes (DEGs) were identified. In addition, 11 enzyme coding genes, 21 transporter genes and 5 MYB transcription factor genes involved in malic acid accumulation were identified. Our combined transcriptomic and targeted metabolomic analyses provides new comprehensive insights into understanding the genetic control of fruit acidity in plum which will be useful for future breeding.

Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly proteins.

Copper is an essential mineral nutrient that provides the cofactors for some key enzymes. However, excess copper is paradoxically cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in many organs, with high mortality and disability. Nevertheless, many questions about the molecular mechanism in Wilson's disease remain unknown and there is an imperative need to address these questions to better exploit therapeutic strategy. In this study, we constructed the mouse model of Wilson's disease, ATP7A-/- immortalized lymphocyte cell line and ATP7B knockdown cells to explore whether copper could impair iron-sulfur cluster biogenesis in eukaryotic mitochondria. Through a series of cellular, molecular, and pharmacological analyses, we demonstrated that copper could suppress the assembly of Fe-S cluster, decrease the activity of the Fe-S enzyme and disorder the mitochondrial function both in vivo and in vitro. Mechanistically, we found that human ISCA1, ISCA2 and ISCU proteins have a strong copper-binding activity, which would hinder the process of iron-sulfur assembly. Of note, we proposed a novel mechanism of action to explain the toxicity of copper by providing evidence that iron-sulfur cluster biogenesis may be a primary target of copper toxicity both in cells and mouse models. In summary, the current work provides an in-depth study on the mechanism of copper intoxication and describes a framework for the further understanding of impaired Fe-S assembly in the pathological processes of Wilson's diseases, which helps to develop latent therapeutic strategies for the management of copper toxicity.

Iron Chelation and Antioxidant Properties of Withania somnifera (Ashwagandha) Restore Fertility in Men and Women

Abstract: Withania somnifera (Ashwagandha) is one of the most renowned and revered medicinal plants in the Indian Ayurvedic system of medicine. Ashwagandha Rasayanas (tonics), capsules, tablets, and powdered herbs (churna) have been used for curing a wide variety of ailments, including reproductive problems, and for improving fertility in men and women as well as erectile dysfunction (ED) in men. Iron accumulation in reproductive organs is caused by excessive dietary intake of iron, dysregulation of iron transporters, chronic blood transfusions, and hemochromatosis. Iron overload produces oxidative stress and causes atrophy of ovaries and testes and hypogonadism, which leads to infertility in men and women. Emerging evidence from preclinical and clinical studies suggests that excessive iron-induced infertility results from dysfunction of the hypothalamic-pituitary-gonadal axis and consequently perturbs the secretion of sex hormones (GnRH, FSH, LH, estrogen, progesterone, and testosterone). The focus of this review is to summarize the pathophysiology of iron-overload toxicity of reproductive organs and the reversal of male/female infertility and libido with Ashwagandha. The bioactive ingredients of Ashwagandha appear to restore iron–overload infertility by acting on iron chelation and capturing iron free radicals (Fe+++) produced by the Fenton reaction. Many synthetic drugs have been tried for treating iron overload infertility, but the outcome has been inconsistent. Considering the high cost of these drugs, Ashwagandha may be a safer and more costeffective phytomedicine to cure iron-overload infertility and enhance libido in humans. Collectively, the iron chelation and antioxidant effects of Ashwagandha seem to reverse iron-overload infertility in men and women by improving testicular and ovarian functions.