ABSTRACT Rice yield and whole biomass production are strongly related to the rice cultivar’s nitrogen (N) uptake ability and various meteorological conditions and management practices. Under different conditions, such as elevated CO2 with limited N-supply, the Indica cultivar” Takanari (Tak)” showed a higher N-uptake ability than the Japonica cultivar ‘Koshihikari (Kos).’ However, it is still unclear how Tak and Kos respond to N-uptake and biomass production under limited N conditions with or without weeding. For this reason, we conducted a pot experiment at Tsuruoka, Yamagata, Japan, in the 2022 rice growing season. Tak and Kos were transplanted individually and interplanted (Tak+Kos) in pots filled with 8.0 kg of air-dried alluvial soil from an organic rice farm with the main treatments of weeding and no weeding. Rice growth parameters (shoot height, tiller numbers, and leaf color) were investigated weekly, while rice biomass and N-uptake were measured after harvest. As a result, plant biomass and N-uptake were significantly different among all treatments. The rice biomass and N-uptake in weeding were significantly higher than those in no-weeding for both cultivars. Compared with Kos, Tak had significantly higher rice biomass and N-uptake for root, ear, and whole rice plants in all treatments, weeding practices, and planting modes. The ratios of aboveground biomass and N-uptake between Tak and Kos in interplanting were higher than their respective ratios in individual planting. Interplanting increased the aboveground biomass ratios between Tak and Kos by 16.24% and 27.43% in weeding and no-weeding conditions, respectively. The ratios of N-uptake in interplanting were higher than individual planting at 21.74% and 34.09% in weeding and no-weeding, respectively. Tak’s ability to compete with Kos on N-uptake and biomass production was increased when two rice cultivars were transplanted in the same pot under interplanting cultivation and no-weeding conditions.
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