Order Polyporales Research Articles

Structure of insect community in the fungus Inonotus radiatus in riparian boreal forests

ABSTRACTBasidiomes of polypore fungi host many insects. Yet systematic information about insect assemblages from most fungal species is lacking. We studied the insect community associated with the wood-decaying fungus Inonotus radiatus (Sowerby: Fr.) P. Karst. (Hymenochaetales). More specifically, we studied the effect of successional stage and weight of basidiomes, as well as shore exposition (north or south), on species richness and composition, as well as occurrence and abundance of the most abundant fungivores. Basidiomes were collected from riparian forests at five lakes in Sweden. Insects were reared out from the basidiomes in the laboratory. A total of 5645 adult insect individuals of 117 taxa were obtained. Among these, 2782 specimens of 36 taxa use Inonotus radiatus basidiomes as breeding habitat. Eight species of parasitic wasps were new to Sweden. The most abundant fungivore was Ennearthron cornutum (Ciidae), which is a generalist breeding in many polypore species. Based on our material and literature, the melandryid beetles Abdera affinis and A. flexuosa seem to be specialists on the order Hymenochaetales. Other frequent fungivores were Dorcatoma dresdensis (Ptinidae), and the lepidopterans Archinemapogon yildizae, Nemapogon cloacellus and N. picarellus (Tineidae). The parasitoid community associated with the tineid moths was similar to the community associated with the other polypore species in the order Polyporales. In contrast, the parasitoids associated with Dorcatoma (and/or Abdera) were different from the other Polyporales species, suggesting that the fungal host species is more important for these parasitoid species than the beetle host species itself. The most abundant and frequent parasitoids were the braconids Diospilus dispar and Colastes fritzeni, which both parasitise Dorcatoma. Species richness was significantly smaller in fresh than in more decayed basidiomes, but species composition did not differ. There was no difference in species composition or richness between north and south shorelines.

Diversity of Polypore Bracket Mushrooms, Polyporales (Agaricomycetes), Recorded in Armenia and Their Medicinal Properties.

The list of polypore bracket mushrooms (Polyporales) recorded in Armenia is presented. The order Polyporales in Armenia is currently represented by 87 species (4 varieties) belonging to 47 genera. Information regarding the study of the medicinal properties (e.g., antifungal, antibacterial, mitogenic, regenerative, antioxidant, proteolytic) of genetically identified mycelial collections of several polypore species-mainly from the genera Daedalea, Fomes, Fomitopsis, Ganoderma, Laetiporus, Piptoporus, Polyporus, and Trametes-is reported, as well.

Wood and humus decay strategies by white-rot basidiomycetes correlate with two different dye decolorization and enzyme secretion patterns on agar plates

During several forays for ligninolytic fungi in different Spanish native forests, 35 white-rot basidiomycetes growing on dead wood (16 species from 12 genera) and leaf litter (19 species from 10 genera) were selected for their ability to decolorize two recalcitrant aromatic dyes (Reactive Blue 38 and Reactive Black 5) added to malt extract agar medium. In this study, two dye decolorization patterns were observed and correlated with two ecophysiological groups (wood and humus white-rot basidiomycetes) and three taxonomical groups (orders Polyporales, Hymenochaetales and Agaricales). Depending on the above groups, different decolorization zones were observed on the dye-containing plates, being restricted to the colony area or extending to the surrounding medium, which suggested two different decay strategies. These two strategies were related to the ability to secrete peroxidases and laccases inside (white-rot wood Polyporales, Hymenochaetales and Agaricales) and outside (white-rot humus Agaricales) of the fungal colony, as revealed by enzymatic tests performed directly on the agar plates. Similar oxidoreductases production patterns were observed when fungi were grown in the absence of dyes, although the set of enzyme released was different. All these results suggest that the decolorization patterns observed could be related with the existence of two decay strategies developed by white-rot basidiomycetes adapted to wood and leaf litter decay in the field.

Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes

The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) were sequenced to expand our knowledge on the diversity of ligninolytic and related peroxidase genes in this Basidiomycota order that includes most wood-rotting fungi. The survey was completed by analyzing the heme-peroxidase genes in the already available genomes of seven more Polyporales species representing the antrodia, gelatoporia, core polyporoid and phlebioid clades. The study confirms the absence of ligninolytic peroxidase genes from the manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP) families, in the brown-rot fungal genomes (all of them from the antrodia clade), which include only a limited number of predicted low redox-potential generic peroxidase (GP) genes. When members of the heme-thiolate peroxidase (HTP) and dye-decolorizing peroxidase (DyP) superfamilies (up to a total of 64 genes) also are considered, the newly sequenced B. adusta appears as the Polyporales species with the highest number of peroxidase genes due to the high expansion of both the ligninolytic peroxidase and DyP (super)families. The evolutionary relationships of the 111 genes for class-II peroxidases (from the GP, MnP, VP, LiP families) in the 10 Polyporales genomes is discussed including the existence of different MnP subfamilies and of a large and homogeneous LiP cluster, while different VPs mainly cluster with short MnPs. Finally, ancestral state reconstructions showed that a putative MnP gene, derived from a primitive GP that incorporated the Mn(II)-oxidation site, is the precursor of all the class-II ligninolytic peroxidases. Incorporation of an exposed tryptophan residue involved in oxidative degradation of lignin in a short MnP apparently resulted in evolution of the first VP. One of these ancient VPs might have lost the Mn(II)-oxidation site being at the origin of all the LiP enzymes, which are found only in species of the order Polyporales.

Genomewide annotation and comparative genomics of cytochrome P450 monooxygenases (P450s) in the polypore species Bjerkandera adusta, Ganoderma sp. and Phlebia brevispora

Genomewide annotation of cytochrome P450 monooxygenases (P450s) in three white-rot species of the fungal order Polyporales, namely Bjerkandera adusta, Ganoderma sp. and Phlebia brevispora, revealed a large contingent of P450 genes (P450ome) in their genomes. A total of 199 P450 genes in B. adusta and 209 P450 genes each in Ganoderma sp. and P. brevispora were identified. These P450omes were classified into families and subfamilies as follows: B. adusta (39 families, 86 subfamilies), Ganoderma sp. (41 families, 105 subfamilies) and P. brevispora (42 families, 111 subfamilies). Of note, the B. adusta genome lacked the CYP505 family (P450foxy), a group of P450-CPR fusion proteins. The three polypore species revealed differential enrichment of individual P450 families in their genomes. The largest CYP families in the three genomes were CYP5144 (67 P450s), CYP5359 (46 P450s) and CYP5344 (43 P450s) in B. adusta, Ganoderma sp. and P. brevispora, respectively. Our analyses showed that tandem gene duplications led to expansions in certain P450 families. An estimated 33% (72 P450s), 28% (55 P450s) and 23% (49 P450s) of P450ome genes were duplicated in P. brevispora, B. adusta and Ganoderma sp., respectively. Family-wise comparative analysis revealed that 22 CYP families are common across the three Polypore species. Comparative P450ome analysis with Ganoderma lucidum revealed the presence of 143 orthologs and 56 paralogs in Ganoderma sp. Multiple P450s were found near the characteristic biosynthetic genes for secondary metabolites, namely polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), terpene cyclase and terpene synthase in the three genomes, suggesting a likely role of these P450s in secondary metabolism in these Polyporales. Overall, the three species had a richer P450 diversity both in terms of the P450 genes and P450 subfamilies as compared to the model white-rot and brown-rot polypore species Phanerochaete chrysosporium and Postia placenta.

THE TOTAL MELANINS CONTENT OF BASIDIOMYCETES ORDERS POLYPORALES AND AGARICALES

The aim of the study was to investigate the total content of melanin in carpophorus, mycelia and culture filtrate species of Basidiomycetes. Methods. Extraction of melanin mycological material carried by its alkali hydrolysis 2 N NaOH solution in the ratio of 1:10, followed by precipitation with concentrated HCl. The precipitate after the addition of hydrochloric acid dissolved in 5 ml of 2 N solution of NaOH. The total pigment content was determined by spectrophotometric method and were calculated using a calibration curve (by pyrocatechol). The melanin content in the culture filtrate was determined by the direct colorimetry CF. Results. The total melanin content in fruiting bodies of 50 species of Basidiomycetes, 27 of which are belonging to order Polyporales and 23 to order Agaricales was investigated. The comparative analysis on the basis of the obtained data showed the highest total melanin content in Ganoderma applanatum, Fistulina hepatica and Laetiporus sulfureus сarpophorus. Dynamics of growth and carotenoid synthesis in mycelium and cultural filtrate of 30 strains of 10 basidiomycetes species isolated in mycelial culture was studied on glucosepeptone medium. Conclusion. Selected strains of Fomes fomentarius and Lentinus edodes are promising for further studies to optimize the culture conditions for mycelial melanin origin.

THE COMPARATIVE CHARACTERISTIC OF GENERAL CAROTENOID CONTENT IN SOME SPECIES OF BASIDIOMYCETES

There were investigated the total carotenoid content in fruiting bodies of 50 species of Basidiomycetes, 27 of which are belonging to order Polyporales and 23 to order Agaricales. The highest total carotenoid content in Ganoderma applanatum, Fistulina hepatica and Laetiporus sulfureus сarpophorus was found out. The dynamics of growth and accumulation of carotenoids in the mycelium and cultural filtrate of 24 strains of 8 basidiomycetes species were studied. Promising for the further research to develop the methods for carotenoids mycelial and extracellular origin strains of species Fistulina hepatica and Laetiporus sulphureus were selected.

Xylophilous Agaricomycetes (Basidiomycota) of the Brazilian Cerrado

An updated checklist of xylophilous Agaricomycetes from the Brazilian Cerrado showed 127 species, 22 families and nine orders (Agaricales, Atheliales, Auriculariales, Corticiales, Gloeophyllales, Hymenochaetales, Polyporales, Russulales, and Thelephorales). The new list includes new specimens collected between 2009 and 2011 in Reserva Biológica de Mogi-Guaçu, São Paulo State, Brazil, and a revision of material previously known from Cerrado. Twelve species are mentioned for the first time for Brazil and 51 are recorded as new for the Cerrado.

Polypore diversity in China with an annotated checklist of Chinese polypores

This report is a comprehensive review of polypore investigations in China up to 2011. Nearly 12,000 polypore specimens have been collected from almost all forest types in China during the past 20 years. After examination of these and other herbarium specimens, 704 polypore species belonging to 132 genera in ten orders were identified, and the majority of them found to belong in the orders Polyporales (accounting for 65% of the total Chinese polypores) and Hymenochaetales (25%). According to the author's investigations, 148 species are boreal, 390 are temperate, 395 are subtropical, and 220 are tropical in distribution. The majority of the Chinese polypores are white rot fungi, whereas 98 species (14%) cause a brown rot; 520 species (74%) were found on angiosperm wood, of which 434 species (62%) grew exclusively on angiosperm wood; 459 species (65%) were found on fallen trunks, which seem to be the most frequent substrate for polypores. Based on field investigations and collections, 223, 267, and 214 species are considered as common, occasional, and rare, respectively, and they subsequently account for 32%, 38%, and 30% of the total Chinese polypores. The host, substratum, distribution, and occurrence of each species are listed.

Polypore diversity in China with an annotated checklist of Chinese polypores

This report is a comprehensive review of polypore investigations in China up to 2011. Nearly 12,000 polypore specimens have been collected from almost all forest types in China during the past 20 years. After examination of these and other herbarium specimens, 704 polypore species belonging to 132 genera in ten orders were identified, and the majority of them found to belong in the orders Polyporales (accounting for 65% of the total Chinese polypores) and Hymenochaetales (25%). According to the author's investigations, 148 species are boreal, 390 are temperate, 395 are subtropical, and 220 are tropical in distribution. The majority of the Chinese polypores are white rot fungi, whereas 98 species (14%) cause a brown rot; 520 species (74%) were found on angiosperm wood, of which 434 species (62%) grew exclusively on angiosperm wood; 459 species (65%) were found on fallen trunks, which seem to be the most frequent substrate for polypores. Based on field investigations and collections, 223, 267, and 214 species are considered as common, occasional, and rare, respectively, and they subsequently account for 32%, 38%, and 30% of the total Chinese polypores. The host, substratum, distribution, and occurrence of each species are listed.

Isolation and evaluation of terrestrial fungi with algicidal ability from Zijin Mountain, Nanjing, China

Approximately 60 fungal isolates from Zijin Mountain (Nanjing, China) were screened to determine their algicidal ability. The results show that 8 fungi belonging to Ascomycota and 5 belonging to Basidiomycota have algicidal ability. Of these fungi, Irpex lacteus T2b, Trametes hirsuta T24, Trametes versicolor F21a, and Bjerkandera adusta T1 showed strong algicidal ability. The order of fungal chlorophyll-a removal efficiency was as follows: T. versicolor F21a > I. lacteus T2b > B. adusta T1 > T. hirsuta T24. In particular, T. versicolor F21a completely removed algal cells within 30 h, showing the strongest algicidal ability. The results also show that all 4 fungal species degraded algal cells through direct attack. In addition, most of the tested fungi from the order Polyporales of Basidiomycota exhibited strong algicidal activity, suggesting that most fungi that belong to this order have algicidal ability. The findings of this work could direct the search for terrestrial fungi for bloom control.

New records and checklist of corticioid <I>Basidiomycota</I> from Uruguay

Twenty-eight corticioid basidiomycete species are reported from Uruguay for the first time. An annotated checklist with 110 species of corticioid Basidiomycota recorded from Uruguay is presented based on these new records and an intensive literature search. These species are distributed in 49 genera and 10 orders. The order Polyporales is represented by the most species (40) and the genus Phanerochaete has the most species (11). Hjortstamia fuscomarginata, Hyphoderma rimosum, Phlebia lividina, and P. subserialis are recorded for the first time from South America. For the complete checklist see http://www.mycotaxon.com/resources/weblists.html.

Exothermic processes in industrial-scale piles of chipped pine-wood are linked to shifts in gamma-, alphaproteobacterial and fungal ascomycete communities

Fast growing softwood species such as pine are preferred for biomass-based heat as well as electricity production and stored in large quantities. A newly established outdoor pile of freshly cut pine-wood chips was monitored to analyze exothermic processes. Inside the pile, a mean temperature increase of up to 44 °C was measured after six days of piling which was paralleled by a decrease of O 2-concentration and an increase of CO 2-concentration. Thereafter four additional CO 2 maxima were observed, while O 2-concentration remained at ambient concentration. The fungal community structure remained almost unchanged after four weeks, while the bacterial community structure was characterized by continuous shifts over time. The rapid heating in the early stage of storage corresponded to high relative abundance of microbial strains belonging to the genera Pseudomonas, Luteibacter and Caulobacter, ascomycetous genera Sphaeropsis and Cadophora and basidiomycetous order Polyporales and genus Sistotremastrum. The late stage was composed by a broader diversity of microorganisms, and heating processes inside the wood pile were attributed mainly to physicochemical processes. Taken together, these observations suggest that the early bacterial and fungal communities are key players in exothermic processes and were replaced by a broader diversity of highly adapted microorganisms.

Free-radical Scavenging Activity of some European Polyporales

The ethanol extracts of 30 mushroom species from the order Polyporales were tested for their free radical scavenging activities by means of the DPPH free-radical scavenging and hydrogen peroxide-induced luminol chemiluminescence assays. In the DPPH radical scavenging test, 7 species gave EC50 values of less than 1 mg/mL. The highest activity was exhibited by Fomitopsis pinicola (0.09 mg/mL). However, in the hydrogen peroxide-induced luminol chemiluminescence assay, only 4 species had EC50 values of less than 1 mg/mL, with the highest activity being exhibited also by F. pinicola (0.26 mg/mL). When compared with known antioxidants, all the mushroom extracts exhibited relatively low activity in both assays. The free radical scavenging results appeared not to be correlated with the total phenol contents of the extracts.

Chemical Constituents of Stereum subtomentosum and Two Other Birch‐Associated Basidiomycetes: An Interspecies Comparative Study

Metabolites of the wood-rotting fungus Stereum subtomentosum Pouzar (Basidiomycetes, order Russulales, family Stereaceae) occurring on birch (Betula pendula Roth) trees were phytochemically investigated for the first time. Three main metabolite chemotypes present in MeOH extracts of the fruit bodies, viz. steroids, fatty acids, and water-soluble sugars, were fractionated, isolated, and identified by 1D/2D NMR-spectroscopic analyses, NMR data comparisons, and chemical correlations combined with GC/MS experiments. Thirteen compounds including two 5 alpha,8 alpha-epidioxy steroids, alpha,alpha'-trehalose, D-arabinitol, D-mannitol, and saturated/unsaturated fatty acids, were identified. Differences among S. subtomentosum and two other birch-associated fungal species, Trametes versicolor (L.: Fr.) Pilát, and Piptoporus betulinus (Bull.: Fr.) P. Karst (Basidiomycetes, order Polyporales, family Polyporaceae) were evaluated as regards the richness and abundance relationships in metabolite profiling.

Free radical scavenging activity of mushrooms from order Polyporales

Free radical scavenging activity of mushrooms from order Polyporales