Orchioides Research Articles

Anti-Amnesic Effect of Curculigo orchioides Plant Extract and Its Function on Acetylcholinesterase Enzyme

Background: Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, and one of the most predominant causes of dementia in older people. Preventing acetylcholine from breakdown by an excess amount of acetylcholinesterase is a proven therapy for AD. Traditionally Curculigo orchioides is known for its antioxidant activity, enzyme inhibition activity, and other medicinal uses. In this study, in vitro acetylcholinesterase, inhibitory activity was investigated, and methanolic extract of the plant showed significant activity. This study aims to investigate cholinesterase inhibitory activity, learning, and memory-enhancing activity of Curculigo orchioides.
 Materials and Methods: The crude methanol extract of the dried powder was prepared by the cold extraction method. In-vitro acetylcholinesterase (AChE) inhibitory activities were determined by the modified Ellman’s method. To confirm learning and memory-enhancing effects, the scopolamine-induced memory impairment Swiss-albino mice were used, and to find the anti-amnesic effect of the extract, a passive shock avoidance task was applied.
 Results: Results proved that scopolamine-induced cognitive dysfunction decreased significantly by administration of the plant extract solution in the passive avoidance task, and inhibited brain acetylcholinesterase activity. While running an in-vitro AChE inhibitory test the IC50 was 0.782±0.067μg/ml.
 Conclusion: The results suggested that methanolic extract of Curculigo orchioides can inhibit AChE in-vitro. Besides this, it provides data that proves its ability to improve whole brain AChE activity and enhance memory.

Rapid screening for minimum inhibitory concentration of medicinal plant extracts against human pathogenic bacteria

In the present study, crude extracts of different parts of six medicinal plant species have been screened for antibacterial activity through resazurin dye reduction assay. The crude extracts of Coleus strobilifer, Phyllanthus reticulatus, Acalypha paniculata, Solanum erianthum, Curculigo orchioides, and Spatholobus parviflorus were prepared with the solvents, petroleum ether, methanol and ethanol by Soxhlet extraction method. Resazurin titreplate assay was done for screening the plant extracts for minimum inhibitory concentration (MIC) the pathogenic bacteria, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. The results revealed that the crude extracts of various parts of the plants obtained using three different solvents were capable of inhibiting the test bacteria with the MIC values ranging from 125 μL to >1000 μL. The stem extracts of C. strobilifer and A. paniculata showed the least antibacterial activity with the MIC values >1000 µL. The leaf extracts of S. erianthum showed a MIC value of 250-500 µL against S. aureus, P. aeruginosa and K. pneumoniae. The results revealed that the rhizome extract of C. orchioides and fruit extracts of S. parviflorus have good antibacterial activity and can be tested further with specific trials to develop antibacterial drugs. For initial screening of medicinal plants for antibacterial activity is faster, more accurate and reliable with resazurin dye reduction method.

Optimization of culture conditions for callus proliferation of Curculigo orchioides Gaertn.

Curculigo orchioides Gaertn is a herbaceous plant that has long been used as a tonic in Vietnam with noticeable health benefits. However, the demand for rhizomes of this species could not be met due to their decreasing number in natural habitats. Despite its vulnerability, there are still not enough researches on producing calli of C. orchioides, which is a method having the capacity of creating a large source of cell biomass for bioactive compounds’ extractions. Thus, this study was conducted to figure out the best conditions for C. orchioides’s callus proliferation. Different light regimes, mineral media, and concentrations of some factors like kinetin (KIN), α-naphthaleneacetic acid (NAA), yeast extract (YE), activated charcoal (AC), and silver nitrate (AgNO3) were examined. It is shown by the results that half-strength MS medium (½ MS) given 0.5 mg/L KIN and MS medium supplemented with 0.5 mg/L KIN and put in the dark (0 light hour : 24 dark hours) are the optimal conditions for callus proliferation, with the highest fresh weights (FWs), dry weights (DWs), and growth indices (GIs) of 3.89 g / 0.45 g / 7.78, and 4.10 g / 0.47 g / 8.20, respectively. Additionally, the inhibitory effects of AgNO3, YE, and AC were demonstrated since there was no observed heavy callus in the media containing those factors.

Quantification of macro, micro and trace elements, and antimicrobial activity of medicinal herbs and their products

The study describes the content of macro, micro, and trace essential and toxic elements in thirteen medicinal herbs and their products including Acorus calamus, Blepharis edulis, Caesalpinia bonducella, Curculigo orchioides, Helicteres isora, Holarrhena pubescens, Pastinaca sativa, Pistacia integerrima, Quercus infectoria, Rauwolfia serpentina, Saussurea lappa, Teucrium stocksianum, and Xanthium strumarium available in the local markets of Pakistan. The elemental content were analyzed with the techniques of inductively coupled plasma (ICP) optical emission spectroscopy (OES) and ICP-mass spectrometry (MS). Furthermore, their antibacterial and antifungal activities were evaluated against the selected microbial pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli, Candida albicans, Candida krusei, Aspergillus flavus, and Trichophyton mentagrophytes. Among macro elements, K and Ca showed the highest content, micro elements were in the order of Rb85/ Sr87 > Zn64/Cu63 > Ni60, and among essential trace elements, the content of Cr52/Cr53 and Co59 were high. The content of the analyzed toxic elements were lower than the permissible standard values. The antimicrobial activities against the subject strains were significant with inhibition zones of 7.0-19.0 mm in disc diffusion procedure, and 62.5–1000 µg/mL in minimum inhibitory concentration method. Hence, the presence of nutritional elements at appreciable concentrations, toxic elements within permissible ranges, and significant antimicrobial potential assume the subject herbs as promising nutritional and therapeutic remedies.

Untargeted Metabolomics Analysis Using FTIR and UHPLC-Q-Orbitrap HRMS of Two Curculigo Species and Evaluation of their Antioxidant and α-Glucosidase Inhibitory Activities.

Curculigo orchioides and C. latifolia have been used as traditional medicines such as antidiabetic and anticancer. This study measured the total phenolics and flavonoid contents as well as analyzed the functional groups and chemical compounds using Fourier-transform infrared (FTIR) spectra and UHPLC-Q-Orbitrap-HRMS profiling for the discrimination of plant parts, geographical origin, and compounds that presumably have a significant contribution as antioxidant and α-glucosidase inhibitors on both plants. The total phenolics and flavonoids contents in Curculigo species varied from 142.09 to 452.47 mg gallic acid equivalent (GAE/g) and from 0.82 to 5.44 mg quercetin equivalent (QE/g), respectively. The lowest IC50 for antioxidant and α-glucosidase inhibitory activities is presented by C. latifolia from a higher altitude region. Principal component analysis (PCA) from FTIR and UHPLC-Q-Orbitrap-HRMS data could discriminate the plant parts and geographical origin. Partial least squares (PLS) analysis has identified several functional groups, such as O–H, C–H, C=O, C–C, C–O, and chemical compounds, unknown-185 and unknown-85, that are most likely to contribute to the antioxidant and α-glucosidase inhibitory activities.

Characterization of the complete chloroplast genome of medical plant Curculigo orchioides Gaertn. (Amaryllidaceae)

Curculigo orchioides Gaertn. distributed in subtropical regions of Asia including southern China and India. The plant is used as a traditional medicine in China for the treatment of menorrhagia, osteoporosis, and other gynecological problems. The complete chloroplast genome was reported in this study using the Illumina NovaSeq platform. The whole genome of this species was 157,472 bp in length, with a total GC content of 37.44%. The large single copy (LSC) was 86,507 bp, the small single copy (SSC) was 16,867 bp, and both of the two inverted repeats (IRs) were 27,049 bp, respectively. A total of 132 unique genes were identified, among which are 86 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The phylogenetic analysis revealed that C. orchioides was highly clustered with C. capitulata. Our study will provide useful fundamental data for further phylogenetic and evolutionary studies of C. orchioides.

Studies on antistress activity of Curculigo Orchioides gaertn

The aim of this study was to evaluate the antistress activity of rhizomes ethanolic extract of Curculigo orchioides Gaertn in vivo, using mice with stress-induced injury. The effect of Curculigo orchioides on motor coordination, spontaneous locomotor activity, immobility time, and analgesia was evaluated in mice using standard procedure. The ethanolic extract of Curculigo orchioides at dose levels, i.e., 100 and 200 mg/kg shows pronounced antistress activity evidenced by reduced immobility time in forced swimming and tail test. It also improved motor coordination and enhanced analgesic effect compared to standard diazepam. The Curculigo orchioides was found to be a antistress activity.

Protective effect of Curculigo orchioides in streptozotocin and nicotinamide-induced diabetic neuropathic pain in Wistar rats

Pain, paraesthesia, sensory loss, and associated high morbidity and mortality are typically characterized by diabetic neuropathy (DN). Curculigo orchioides (CO) is a herbal medicine that is rich in flavonoids, glycosides, and polyphenols which are known for potential antioxidant activity. Moreover, the anti-hyperglycemic potential of CO paved the way for pharmacological investigation in the treatment of diabetic complications since the regulation of hyperglycemia is considered as a major factor in the treatment of diabetes. The current study was designed to evaluate the neuroprotective effect of CO extract in DN. DN was induced in rats using streptozotocin (STZ) (65 mg/kg, i.p.) after 15 minutes of nicotinamide (NAD; 230 mg/kg, i.p.). Diabetic rats showed significant mechanical, thermal hyperalgesia, and allodynia. Diabetic rats also showed increased oxidative stress parameters and advanced glycation end products (AGEs) formation. Oral treatment was started on 60th day after STZ–NAD administration and continued for the next 30 days, with different doses (150, 300, and 600 mg/kg) of CO extracts (ethanolic and hydroalcoholic) and standard drug gabapentin (30 mg/kg). The results of the study revealed that both extracts reduced hyperglycemia and inhibited the pain response partially via reduction of oxidative stress and AGEs formation in DN rats. Both the extracts reduced the neuropathic pain in diabetic rats at dose dependent manner.

Temporal heterogeneity in primary and secondary metabolic products of medicinal plants of Sitamata Wildlife Sanctuary in Rajasthan, India

Phyto-chemical investigation in three tuberous medicinal plants, viz., Arisaema tortuosum (Wall.) Schott, Chlorophytum tuberosum (Roxb.) Baker and Curculigo orchioides Gaertn., growing in Sitamata Wildlife Sanctuary of Rajasthan were conducted for both primary and secondary metabolites. Experiment was carried out during June to October to find out monthly variation in accumulation of both primary (total contents of sugars, crude protein and phosphorus) and secondary metabolites (total contents of alkaloids and phenols) in tubers of these three species. In A. tortuosum (Wall.) Schott, crude protein and total alkaloids were found maximum during September, whereas phosphorus and total phenols during July. In C. tuberosum (Roxb.) Baker and C. orchioides Gaertn. maximum values of crude protein, phosphorus and total alkaloids were found during July. It is, therefore, concluded that the maximum production of different metabolites were found to be in the month of July and it is the suitable month for harvesting tubers.

In Vitro Anti-cholinesterase Activity and Mass spectrometric Analysis of Curculigo orchioides Gaertn., Rhizome Extract

Curculigo orchioides Gaertn, is commonly known as golden grass. Acetylcholinesterase is an enzyme involved in non-cholinergic and cholinergic activity in both the central and peripheral nervous systems, most of the AChE is found in a tetrameric form bound to neuronal cell membranes. The AChE induces amyloid-β fibril formation and forms very toxic AChE-Aβ complexes in the brain. The study aims to determine the anticholinesterase activity and chemical composition analysed by GC-MS, LC-MS, FT-IR, and HPTLC analysis in the methanolic extract of C. orchioides Gaertn rhizome. The in vitro anti-acetylcholinesterase activity was performed by bio-autograph and spectrophotometry. The methanolic extract of rhizome has better inhibition of the AChE enzyme in vitro experiments. These results might be useful in explaining the effect of the methanolic extract of the rhizome as an anti-acetylcholinesterase agent for the management of AD.

In vitro antioxidant activity and Content of compounds from Curculigo orchioides rhizome

Curculigo orchioides Gaertn. is used in traditional medicine in Vietnam. Its antioxidant potential was evaluated through DPPH radical scavenging and the total antioxidant capacity method. The data resulted from DPPH radical scavenging activity indicate that Curculigo orchioides display high activity with a low IC50 value (22.78μg/mL), approximately 1.5 times less than that of curcumin (34.34 μg/mL). The total antioxidant capacity of the extract is equivalent to 132.48 ± 1.48 mg GA/g or 264.45 ± 2.34 μmol AS/g. The composition of Curculigo orchioides, including the total phenolic, total flavonoid, polysaccharides, and triterpenoid saponins, was examined by using the colorimetric method with reagents, and their quantity is equivalent to 196.24 ± 1.45 mg GAE/g, 78.49 ± 1.78 mg QE/g, 4.34 ± 0.08 %, 47.60 ± 0.24 mg Rb1/g (Rb1: Gypenoside III), respectively. Specifically, the total triterpenoid saponins content of Curculigo orchioides has been reported for the first time.

KAJIAN ETNOBOTANI BUDAYA NGALAKSA DI KECAMATAN RANCAKALONG KABUPATEN SUMEDANG

The Rancakalong People still hold tight the local culture. Generally, the Rancakalong people are living as farmer. This matter makes the Rancakalong people do the culture of Ngalaksa. This cultural purpose is gratitude of prosperity throught the rice harvest symbolized as Dewi Sri. The purpose of this research is to examine ethnobotany of plants that used in Ngalaksa culture at Rancakalong Sub-district. The method of this research uses descriptive-exploration with the technique of data collection is Pusposive Random Sampling and Snowball Sampling. Ngalaksa culture relates with ethnobotany studies because it uses rice (Oryza sativa L.) as the main plant and other plants such as kecombrang (Etlingera elatior (Jack) R.M.Sm), congkok (Curculigo orchioides Gaertn), cariang (Colocasia esculenta (L.) Schott) and coconut (Cocos nucifera L.). These plants are needed in large quantities and cannot be replace by other plants.

Anti-obesity Activity of Extract of Curculigo orchioides Gaertn. Root in High Fat Diet-induced Obesity in Rats

Curculigo orchioides known as Musali or Talamuli in Ayurveda. Rhizome of C. orchioides is one of a reputed drugs mentioned in the ancient books of Ayurveda and Siddha, Unani and used as an analgesic, anti-inflammatory, spermatogenic, aphrodisiac, immunostimulant, hepatoprotective, anti-asthmatic etc. To investigate the anti-obesity activity of extract of C. orchioides root in high fat diet-induced obesity rats. The five groups in this study were, group I (normal), group II received high fat diet, group III received obese rats given orlistat (50 mg/kg, p.o.), groups IV-Obese rats given extract of C. orchioides root 500 mg/kg, p.o. and groups V-Obese rats given extract of C. orchioides 1000 mg/kg, p.o. On 30th day of experiment, blood glucoses level, lipid profile and biochemical parameter were performed. Body weight of animals was significantly (p iss less than 0.05) maintained in all treatment groups. Progressive maintained blood glucose, lipid profile, and oxidative stress biomarkers were found in all treatment groups during study. The obtained results clearly suggested that C. orchioides possess pronounced anti-obesity potential

New chlorophenolic glycoside from Curculigo orchioides and their activities on 5α-reductase

Two new chlorophenolic glucosides curculigines P (1) and Q (2), together with seven known compounds (3–9), were isolated from the dried rhizomes of Curculigo orchioides. Their structures were determined by spectroscopic methods including 1 D, 2 D NMR and MS. All the isolated compounds were evaluated on 5α-reductase activity by a HaCaT-based bioassay. Compounds 1–9 showed varying degrees of inhibiting activity against 5α-reductase, while compound 1 indicated the most potent inhibitory effect.

A Kinetic Study of Acetylcholinesterase Inhibition by Fractions of Oleo diox Roxb.leaf and Curculigo orchioides Gaertn Rhizome for the Treatment of Alzheimer’s Disease

Introduction and Aim: Acetylcholinesterase (AChE) has an important role in the biochemical studies indicating that AChE accelerates the formation of amyloid-β fibril and forms AChE-Aβ complexes in the brain. The medicinal plants of Curculigoorchioides Gaertn., and Oleo diox Roxb. collected from Western Ghat region of Kodagu, Karnataka, India have many medicinal values such as antioxidant, anti-cancer, anti-diabetic, neuroprotective activity and anti-inflammatory property. Aim of the study was to check whether C. orchioides Gaertn. and Oleo diox Roxb. fractions significantly inhibit acetylcholinesterase in different concentration-dependent manner(1-3 mg/mL).
 Results: Plant fraction concentrations resulted in >60%AChE inhibition as compared with the standard Galantamine. The IC50 value was 0.15 mg/mL which was calculated from the equation of the percentage inhibition curve for the test or plant fraction. The Lineweaver-Burk plot indicated that the fraction followed competitive and non-competitive inhibition kinetics. Finally, we conclude that the type of plot exhibited by the C. orchioides Gaertn. and Oleo dioxRoxb. fractions has a possible therapeutic application for improving memory and other cognitive functions.The medicinal plant-based drugs are known to have limitations due to their fewer side effects and problems associated with bioavailability, which necessitates the interest in finding better AChE inhibitors from plant sources.

Phytochemical analysis of Curculigo orchioides and its cytotoxic effect on lung adenocarcinoma cancer cell line (NCI-H522)

Curculigo orchioides commonly called as ‘Kali Musli’ is an endangered medicinal plant, extensively used in Indian and Chinese indigenous medicines. The plant has several medicinal properties like anti-inflammatory, anti-diabetic, anti-cancer etc. The present study aims to examine the cytotoxic activity of aqueous and methanolic extract of roots and leaves of C. orchioides on human lung adenocarcinoma NCI-H-522 cancer cell line. In-vitro cytotoxicity was determined by MTT assay. The tests were conducted using various doses of the plant extracts. The crude extracts did not show IC50 even at 500 μg/ml when tested on NCI-H-522 cancer cell line in vitro. Our present finding indicate that aqueous fresh root extracts and methanolic dried root extracts are significantly toxic as compared to leaf extracts. Cytotoxic property of the plant root is attributed to the presence of alkaloids and phenols.

Curculigoside Protects against Excess-Iron-Induced Bone Loss by Attenuating Akt-FoxO1-Dependent Oxidative Damage to Mice and Osteoblastic MC3T3-E1 Cells.

Summary The present investigation found that curculigoside (CUR) can prevent excess-iron-induced bone loss in mice and cells through antioxidation and inhibiting excess-iron-induced phosphorylation of the Akt-FoxO1 pathway. CUR can attenuate the decreasing of cell viability, enhance autophagy, potentiate the antioxidant effect, and reduce apoptosis in MC3T3-E1 cells treated with excess iron through regulating the expression of FoxO1 target gene. Introduction Oxidative stress induced by iron overload is an important factor involved in primary osteoporosis disease and iron overload-related diseases. Curculigoside (CUR), a phenolic glycoside found abundantly in Curculigo orchioides Gaertn., has been demonstrated to possess antioxidant and antiosteoporotic properties. The aim of the present study is to explore the underlying molecular mechanism of CUR on excess-iron-induced bone loss in mice and osteoblastic MC3T3-E1 cells. Methods An iron-overload mice model was used to study the protective effects of CUR on bone loss induced by oxidative stress. Serum bone metabolism markers and antioxidant enzymes were also measured. To explore the antioxidant mechanism of CUR, the MC3T3-E1 osteoblastic cell line was used. Results In vivo studies showed that BMD and microarchitectural parameters were improved after a 3-month administration of CUR. CUR improved the biochemical parameters related to bone metabolism and the expressions of Runx2, OCN, and type 1 collagen and increased the formation of bone-mineralized nodules in vitro. CUR also inhibited ROS generation and increased the activities of antioxidant enzymes both in vivo and in vitro treated with excess iron. CUR can upregulate the level of FoxO1 and Nrf2, downregulate the level of p53 and the phosphorylation level of FoxO1, improve nuclear translocation of FoxO1, probably by inhibiting the IGFR/AKT signaling pathway, then increased cell viability and autophagy, and reduced apoptosis of MC3T3-E1 cells treated with excess iron by regulating the expression of FoxO1 target genes MnSOD, Gadd45a, Bim, FasL, and Rab7. Conclusions These results demonstrated that CUR was able to alleviate bone loss induced by oxidative stress resulting from iron overload, suggesting its potential use for the treatment of primary osteoporosis and bone loss in iron-overload-related diseases.

Therapeutic Anabolic and Anticatabolic Benefits of Natural Chinese Medicines for the Treatment of Osteoporosis.

Osteoporosis is a bone disease characterized by increasing osseous fragility and fracture due to the reduced bone mass and microstructural degradation. Primary pharmacological strategies for the treatment of osteoporosis, hormone replacement treatment (HRT), and alendronate therapies may produce adverse side-effects and may not be recommended for long-term usage. Some classic and bone-specific natural Chinese medicine are very popularly used to treat osteoporosis and bone fracture effectively in clinical with their potential value in bone growth and development, but with few adverse side-effects. Current evidence suggests that the treatments appear to improve bone metabolism and attenuate the osteoporotic imbalance between bone formation and bone resorption at a cellular level by promoting osteoblast activity and inhibiting the effects of osteoclasts. The valuable therapies might, therefore, provide an effective and safer alternative to primary pharmacological strategies. Therefore, the purpose of this article is to comprehensively review these classic and bone-specific drugs in natural Chinese medicines for the treatment of osteoporosis that had been deeply and definitely studied and reported with both bone formation and antiresorption effects, including Gynochthodes officinalis (F.C.How) Razafim. & B.Bremer (syn. Morinda officinalis F.C.How), Curculigo orchioides Gaertn., Psoralea corylifolia (L.) Medik Eucommia ulmoides Oliv., Dipsacus inermis Wall. (syn. Dipsacus asperoides C.Y.Cheng & T.M.Ai), Cibotium barometz (L.) J. Sm., Velvet Antler, Cistanche deserticola Ma, Cuscuta chinensis Lam., Cnidium monnieri (L.) Cusson, Epimedium brevicornum Maxim, Pueraria montana (Lour.) Merr. and Salvia miltiorrhiza Bunge., thus providing evidence for the potential use of alternative Chinese medicine therapies to effectively treat osteoporosis.

Determination of orcinol glucoside by LC-MS in Curculigo orchioides and its application to a pharmacokinetic study

This study was designed to explore the pharmacokinetic regularity of the plasma concentration, tissue distribution and excretion of orcinol glucoside from aqueous extracts of raw and processed Curculigo orchioides Gaertn., Hypoxidaceae. The experiment first used an ultrahigh-performance liquid chromatography-tandem mass spectrometry approach with multiple reaction monitoring and a positive mode to separate orcinol glucoside from naringin to obtain the plasma concentration curves, bar graph of tissue distribution and excretion curves. These results might be beneficial for reasonable clinical application of C. orchioides and for further development of its wine and salt-processing mechanism.

Evaluation of antioxidant and hepatoprotective property of ethanolic extract of curculigoorchioides in albino rats

Background: Liver, the largest organ invertebrate body, it the major site of intense metabolic activities. Being the largest chemical factory of the body, the liver performs innumerable functions. The functional units of liver are called either hepatic or classic lobules or acini. The hepatic lobules consist largely of parenchymal or epithelial glandular cells called hepatocytes, which are arranged as interconnected plates or laminae.Methods: All the animal experiments were conducted according to the ethical norms approved by Ministry of Social Justices and Empowerment, Government of India, and Institutional Animal Ethical Committee guidelines. Fifteen rats were selected for this study and randomly divided into three groups of five each. Group I include control rats that received isocarolic quantity of sucrose in the same volume as experimental rats that received ethanol. Group II includes ethanol treated rats. Ethanol was administered daily at regular intervals by gastric intubation at a dose of as 20% aqueous solution 1 ml for 60 days. Group III includes ethanol (1ml of 25%) + Curculigoorchioides rhizome (80mg/kg b.wt) treated rats for 60 days, daily at regular intervals by gastric intubation.Results: The effect of ethanol alone and ethanol+curculigoorchioides rhizome extract (combined) on adult male rat liver metabolism were assessed in the present study and compared with the control rate. The mean body weight of ethanol treated rats was lesser than control and ethanol+curculigoorchioides rhizome extract treated rats. (Table 1, Figure 1) Data on liver weight from control, ethanol alone and ethanol+curculigoorchioides extract (combined) treated rats did not show much variation (Table 1, Figure 2).Conclusions: The effect of curculigo orchioides rizhomes extract+ethanol (combined) on adult male rat liver metabolism was assessed in the present study and compared with control and ethanol alone treated rats. The administration of ethanol accelerates the glycogenolysis and drastically reduced the hepatic glycogen content. The hepatic transaminase activity was swayed by ethanol treatment and reverted to normalcy by combined treatment. Elevated levels of serum enzyme are indicative of cellular leakage and loss of functional integrity of cell membrane in liver. The reversal of altered transaminase activities to normal by plant extract supplementation suggest its hepatoprotective action.