Oral Mucosal Immunity Research Articles

Inborn Errors of Immunity-related Immunological Mechanisms and Pharmacological Therapy Alternatives in Periodontitis.

Periodontitis is a frequent local inflammatory disease. The microbiota and repeated exposure to bacterial endotoxins triggers excessive inflammation through oral mucosal immunity, sometimes leading to a destructive effect on the supportive mucosal tissues around the teeth. Elimination of the pathogens and increasing the tolerance of the cellular immune response is crucial in addition to standard dental therapies like mechanical debridement. Based on our experience on immune-mediated diseases, especially primary immunodeficiency diseases (PIDs), we wrote this review to discuss the treatment alternatives for severe periodontal disease. Risk factors are malnutrition, vitamin deficiencies, smoking, systemic inherited and acquired immune-mediated diseases, infections, endocrinological diseases, and pharmacological agents may accompany periodontitis. The diagnosis and treatment of dietary deficiencies, as well as the addition of nutritional supplements, may aid in epithelial regeneration and immune system function. Recently, modifications to the therapeutic option for severe periodontitis have been made depending on the fact that the immune response against bacteria may modify the severity of periodontal inflammation. The anti-inflammatory therapies support or inhibit the host's immune response. The clinical approach to severe periodontitis should extend beyond classical therapies. There is a need for a diverse therapeutic strategy that supports the epithelial barrier, which is the crucial component of innate immunity against microbiota. Leukocytes are the main cellular component in periodontal inflammation. Anti-inflammatory therapeutic options directed at leukocytes, such as IL-17 and IL-23-targeted therapies, could be the candidates for the treatment of severe periodontitis. Therapy against other inflammatory cytokines, IL-1, IL-6, IL12, IL23, TNF-alpha, PGE2, and cytokine receptors, could also be used in periodontal inflammation control.

Changes in the levels of cytokines IL-1β, IL-4, IL-8, and IL-10 in dental orthopedic fixed prosthetics

Despite significant advances in materials science, introduction of new methods and principles of prosthetic treatment into the practice of dentists, complications after prosthetics with fixed structures are encountered. The result of dental prosthetics of patients with defects of hard tissues of teeth depends not only on the chosen construction and materials from which they are made, but is closely connected with the functional activity of the immune system. Cytokines are an important factor of mucosal immunity. The cytokine system represents one of the key components of mucosal immunity. It regulates normal physiological functions, restoration of haemostasis in the interaction of physical, chemical and biological factors on the body and participates in the pathogenesis of allergic, autoimmune, and autoinflammatory processes. The aim of the study is to compare the content of IL-1â, IL-8, IL-10, and IL-4 in unstimulated saliva and gingival fluid in patients during adaptation to metal-ceramic and oxide ceramic tooth-supported constructions. In the period from 2021-2024, we examined 3 groups of patients aged 46±2 years: group 1 – comparison group, patients without dental constructions in the oral cavity (25 people); group 2 – patients treated with tooth-supported metal-ceramic (MC) crowns (25 people); and group 3 – patients treated with tooth-supported zirconium dioxide (ZrO2) crowns (25 people). On the basis of IL-1â, IL-8, IL-10, and IL-4 we concluded that the mucosal immunity of oral mucous membranes is less affected by the structures made of oxide ceramics compared to the crowns made of metal-ceramics. Similar patterns of changes in the levels of IL-1â, IL-8, and IL-4 in saliva and gingival fluid in patients who underwent dental rehabilitation were found, which allows us to use only saliva in order to eliminate the complexity and specificity of gingival fluid sampling. Also, changes in cytokine indices in saliva and gingival fluid at different stages of prosthodontics reflect the level of reaction of oral mucosal immunity, which can serve as a criterion of the quality of the performed dental treatment.

Animal models to study the pathogenesis and novel therapeutics of oral lichen planus.

Oral lichen planus (OLP) is a prevalent oral mucosal disease characterized by an unknown etiology and a complex pathogenesis. Patients with OLP endure a chronic course marked by alternating non-erosive and erosive lesions, with no definitive cure currently available. Particularly challenging is the treatment of recalcitrant erosive OLP, highlighting an urgent need for therapies targeting specific pathogenic pathways. In diseases like OLP, where the etiopathogenesis is intricate and elusive, animal models are indispensable for hypothesis testing and elucidating disease mechanisms. To date, only three animal models for oral lichenoid lesions have been reported in the literature. This Perspective paper evaluates these existing models, along with a novel OLP mouse model introduced at the 3rd International Conference on Oral Mucosal Immunity and Microbiome. The validity of these models is critically assessed, and their potential future applications in advancing our understanding of OLP are discussed.

Inhibition of METTL3 Alleviates NLRP3 Inflammasome Activation via Increasing Ubiquitination of NEK7.

N6-methyladenosine (m6A) modification, installed by METTL3-METTL14 complex, is abundant and critical in eukaryotic mRNA. However, its role in oral mucosal immunity remains ambiguous. Periodontitis is a special but prevalent infectious disease characterized as hyperinflammation of oral mucosa and bone resorption. Here, it is reported that genetic deletion of Mettl3 alleviates periodontal destruction via suppressing NLRP3 inflammasome activation. Mechanistically, the stability of TNFAIP3 (also known as A20) transcript is significantly attenuated upon m6A modification. When silencing METTL3, accumulated TNFAIP3 functioning as a ubiquitin-editing enzyme facilitates the ubiquitination of NEK7 [NIMA (never in mitosis gene a)-related kinase 7], and subsequently impairs NLRP3 inflammasome assembly. Furtherly, Coptisine chloride, a natural small-molecule, is discovered as a novel METTL3 inhibitor and performs therapeutic effect on periodontitis. The study unveils a previously unknown pathogenic mechanism of METTL3-mediated m6A modifications in periodontitis and indicates METTL3 as a potential therapeutic target.

Polysaccharide from Atractylodes macrocephala Koidz Binding with Zinc Oxide Nanoparticles as a Novel Mucosal Immune Adjuvant for H9N2 Inactivated Vaccine.

H9N2 avian influenza poses a significant public health risk, necessitating effective vaccines for mass immunization. Oral inactivated vaccines offer advantages like the ease of administration, but their efficacy often requires enhancement through mucosal adjuvants. In a previous study, we established a novel complex of polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles (AMP-ZnONPs) and preliminarily demonstrated its immune-enhancing function. This work aimed to evaluate the efficacy of AMP-ZnONPs as adjuvants in an oral H9N2-inactivated vaccine and the vaccine's impact on intestinal mucosal immunity. In this study, mice were orally vaccinated on days 0 and 14 after adapting to the environment. AMP-ZnONPs significantly improved HI titers, the levels of specific IgG, IgG1 and IgG2a in serum and sIgA in intestinal lavage fluid; increased the number of B-1 and B-2 cells and dendritic cell populations; and enhanced the mRNA expression of intestinal homing factors and immune-related cytokines. Interestingly, AMP-ZnONPs were more likely to affect B-1 cells than B-2 cells. AMP-ZnONPs showed mucosal immune enhancement that was comparable to positive control (cholera toxin, CT), but not to the side effect of weight loss caused by CT. Compared to the whole-inactivated H9N2 virus (WIV) group, the WIV + AMP-ZnONP and WIV + CT groups exhibited opposite shifts in gut microbial abundance. AMP-ZnONPs serve as an effective and safe mucosal adjuvant for oral WIV, improving cellular, humoral and mucosal immunity and microbiota in the gastrointestinal tract, avoiding the related undesired effects of CT.

The experience of treating chronic peri-implantitis in HIV positive patients

Relevance. There is a scarcity of data regarding the treatment of chronic peri-implantitis in HIV-positive patients. The refinement of available techniques for managing inflammation around dental implants holds significant importance, particularly in individuals infected with HIV. Hence, this study aims to consolidate clinical experiences in managing chronic peri-implantitis in HIV-positive patients.Materials and methods. The study involved six male patients (treatment group), aged 35 to 54, diagnosed with chronic peri-implantitis, exhibiting inflammation around dental implants and testing HIV positive. These patients, under dynamic observation by their infectious disease specialist, were consistently receiving antiretroviral treatment. The control group consisted of 10 males aged 28 to 55, devoid of HIV or any other condition causing secondary immunodeficiency. Oral cavity microbiota and mucosal immunity were examined, and treatment for chronic peri-implantitis was administered. Both groups had dental implants for 5 to 7 years. The specifics of the implantation systems were not explored.Results. HIV-positive patients with peri-implantitis exhibited alterations in microbiota and mucosal immunity, correlating with "viral load" indicators. The study presented combined treatment outcomes for periodontitis and the influence of adjusted antiretroviral therapy on mucosal immunity and oral cavity microbiota parameters.Conclusion. Peri-implantitis onset in HIV-positive patients with adequate oral hygiene might be linked to an underlying factor involving ineffective administration of antiretroviral treatment. Consequently, diagnosing periimplantitis in HIV-positive individuals should not only entail appropriate therapeutic and preventive measures as part of a comprehensive peri-implantitis treatment regimen, along with precise oral hygiene correction performed professionally, but also timely referral to an infectious disease specialist for adjustment of antiretroviral therapy.

Influence of using a domestic adhesive cream for fixation of removable dentures with plantain extract on mucosal microbiome and secretory immunity of the mouth

Relevance. Denture stomatitis, including fungal infection, which affects many wearers of removable dental prostheses, has a multifactorial etiology with poor oral hygiene as the main predisposing factor. Therefore, the search for means that contribute to the prevention of occurrence and elimination of denture stomatitis in persons using removable dental prostheses remains relevant.Objective. The effect of the adhesive cream for fixation of dentures Asepta Рarodontal with plantain extract on the mucosal microbiome and secretory immunity of the mouth of patients using removable dentures was evaluated.Materials and Methods. The content of secretory immunoglobulin A (sIgA) in saliva was studied by enzyme-linked immunosorbent assay and microbiological examination for periodontopathogens (Prevotella intermedia, Bacteroides forsythus, Treponema denticola, Actinobacillus actinomycetemcomitans, Porhyromonas gingivalis), as well as Streptococccus spp, Staphylococcus spp and Candida spp in 3 groups of patients who did not use any dental prostheses (1 control group, 30 people). ), as well as those who were made partial removable acrylic dentures from the first day of application were offered to use domestic adhesive cream for fixation of dentures Asepta Рarodonta (2 main group, 30 people) and who were made partial removable acrylic dentures, but their adaptation period and subsequent use of dentures passed without the use of any adhesive means for fixation of dentures (3 comparison group, 30 people).Results. It was found that after 6 months of application of adhesive cream for fixation of dentures with plantain extract there was a significant decrease in detection of Bacteroides forsythus and Porphyromonas gingivalis in comparison with the control group, as well as a significant increase in the level of sIgA, which was noted 1 month after the beginning of application of Asepta Carodontal denture fixation agent and continued after 6 months. This indicates the strengthening of secretory immunity, which leads to increased binding of microbes in saliva with their further excretion, inhibition of adhesion of C. albicans to the walls of the denture and epithelial cells of the oral mucosa, which ultimately contributes to a decrease in colonization of the oral mucosa epithelium by fungi and a decrease in the incidence of stomatitis.Conclusion. Prevention of denture stomatitis by optimizing the microbiome of the oral mucosa and mucosal immunity is possible due to the use of adhesive cream for fixation of dentures Asepta Carodontal, as it creates the possibility of preserving and optimizing the microbiocenosis and immune balance in the oral cavity after dental prosthetics, which allow us to recommend the use of adhesive agents for fixation of removable dental prostheses, including cream Asepta Carodontal.

Insufficient anti-spike RBD IgA responses after triple vaccination with intramuscular mRNA BNT162b2 vaccine against SARS-CoV-2

ObjectivesThis study aims to examine whether the parenterally administered mRNA-based COVID-19 vaccines can induce sufficient mucosal-type IgA responses to prevent SARS-CoV-2 transmission. MethodsWe examined the longitudinal kinetics of SARS-CoV-2 spike RBD-specific IgA and IgG responses in sera of Japanese healthcare workers (HCWs) after receiving two doses and the third dose of BNT162b2 mRNA vaccines. During the prospective cohort study, Omicron breakthrough infections occurred in 62 participants among 370 HCWs who had received triple doses of the vaccine. Pre-breakthrough sera of infected HCWs and non-infected HCWs were examined for the levels of anti-RBD IgA and IgG titers. ResultsThe seropositivity of anti-RBD IgA at 1 M after the second vaccine (2D-1M) and after the third dose (3D-1M) was 65.4% and 87.4%, respectively, and wanes quickly. The boosting effect on anti-RBD Ab titers following breakthrough infections was more notable for anti-RBD IgA than for IgG. There were partial cause-relationships between the lower anti-RBD IgA or IgG at pre-breakthrough sera and the breakthrough infection. ConclusionsParenterally administered COVID-19 vaccines do not generate sufficient mucosal-type IgA responses despite strong systemic IgG responses to SARS-CoV-2. These results demonstrate the necessity and importance of reevaluating vaccine design and scheduling to efficiently increase oral or respiratory mucosal immunity against SARS-CoV-2.

Influence of inhaled bacteriophage therapy on oral mucosal immunity in children with acute tonsillitis

The study aimed to assess the effect of inhaled bacteriophage therapy on oral mucosal immunity in children with acute tonsillitis.
 Materials and methods.We examined 212 patients aged 4 to 15 years old with acute tonsillitis and 110 age-matched apparently healthy children. Research methods: calculating the Neutrophil to lymphocyte ratio (NLR), saliva diagnostics — secretory immunoglobulin A (sIgA) and the pro-inflammatory cytokine (TNFα). Taking into account the scheme of the treatment, the patients were divided into mutually comparable groups: the first group included patients with acute tonsillitis who received the standard generally accepted treatment depending on the clinical form of the pathology, without using bacteriophage therapy — n = 107 (50.5%), the second group — patients receiving a course of bacteriophage therapy — n = 105 (49.95%), nebulizer bacteriophage therapy using liquid complex pyobacteriophage (PCL) (Microgen, Russia) from the first days of the disease along with standard treatment.Results.During bacteriophage therapy, on the 6th day of treatment, an increased sIgA level up to 97.2% was observed particularly in younger and adolescent patients up to 97.2% (p ≤ 0.05). At the same time, this parameter reached 75.8% and 81.6%, respectively (p ≤ 0.05), in patients who received only standard treatment. The following difference between the two study groups was observed: between patients in the younger age subgroup — 21.4%, in the older age subgroup -16.1% (p ≤ 0.05 relative to control group), which indicates a more effective drug-related effect in patients from the younger age group groups. Similar changes are observed while analyzing level of the pro-inflammatory cytokine (TNFα).
 Conclusion.The use of inhaled bacteriophage therapy in the combination treatment of children with acute tonsillitis helps to shorten the period of general and local clinical manifestations of the disease by 1.4-fold and improve mean local immunity from 5.7% up to 16.1% (p ≤ 0.05).

Innate adaptive immune cell dynamics in tonsillar tissues during chronic SIV infection.

HIV-infected patients are at higher risk of developing oral mucosal infection and Epstein-Barr virus (EBV)-associated B cell malignancies. However, the potential role of oral immunity in the pathogenesis of oral lesions is unknown. Tonsils are oral-pharyngeal mucosal-associated lymphoid tissues that play an important role in oral mucosal immunity. In this study, we investigated the changes of innate and adaptive immune cells in macaque tonsils during chronic SIV infection. We found significantly higher frequencies of classical monocytes, CD3+CD56+ (NKT-like) cells, CD3+CD4+CD8+ (DP), and CD161+ CD4 T cells in tonsils from chronic infected compared to naïve animals. On the contrary, intermediate monocytes and CD3+CD4-CD8- (DN) cells were lower in chronic SIV-infected macaques. We further confirmed a recently described small B-cell subset, NKB cells, were higher during chronic infection. Furthermore, both adaptive and innate cells showed significantly higher TNF-α and cytotoxic marker CD107a, while IL-22 production was significantly reduced in innate and adaptive immune cells in chronic SIV-infected animals. A dramatic reduction of IFN-γ production by innate immune cells might indicate enhanced susceptibility to EBV infection and potential transformation of B cells in the tonsils. In summary, our observation shows that the SIV-associated immune responses are distinct in the tonsils compared to other mucosal tissues. Our data extends our understanding of the oral innate immune system during SIV infection and could aid future studies in evaluating the role of tonsillar immune cells during HIV-associated oral mucosal infections.

Fish oral vaccine and mucosal immunity

Fish oral vaccine and mucosal immunity

Salivary IgA subtypes as novel disease biomarkers in systemic lupus erythematosus.

Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature. Several autoimmune diseases have been related to diverse abnormalities in oral mucosal immunity, such as rheumatoid arthritis, Sjogren's syndrome, and systemic lupus erythematosus (SLE). We decided to evaluate the levels of both IgA subtypes in the saliva of SLE patients. A light chain capture-based ELISA measured specific IgA1 and IgA2 levels in a cohort of SLE patients compared with age and gender-matched healthy volunteers. Surprisingly, our results indicated that in the saliva of SLE patients, total IgA and IgA1 subtype were significantly elevated; we also found that salivary IgA levels, particularly IgA2, positively correlate with anti-dsDNA IgG antibody titers. Strikingly, we also detected the presence of salivary anti-nucleosome IgA antibodies in SLE patients, a feature not previously reported elsewhere. According to our results and upon necessary validation, IgA characterization in saliva could represent a potentially helpful tool in the clinical care of SLE patients with the advantage of being a more straightforward, faster, and safer method than manipulating blood samples.

The Relationship of Changes in Oral Microbiocenosis and Mucosal Immunity in the Conditions of 14-Day Isolation of a Person in a Hermetic Object with an Artificial Habitat

Background. Changes in the human oral microbiota is an adaptive process. Infections will be particularly manifest in extreme conditions, especially during a long stay in space flight, where the astronaut is exposed to various nonspecific stresses. Aim the aim of the work is to estimate the complex influence of 14-day isolation conditions of human being in sealed environment on the state of natural barriers of periodontal colonization. Methods. During the experiment 6 volunteer subjects (4 men and 2 women) aged 24 to 45 years old were confined for 14 days to an air-tight space simulating a spaceship capsule. Then from 6 to 18 days after leaving the experiment the experimental group (4 people) received Lactobacillus spp. autoprobiotics once a day on an empty stomach in the morning. During this period the control group (2 persons) took Linex (Lebenin: Lactobacillus acidophilus (species L. gasseri) 300 mg, Bifidobacterium infantis 300 mg, Enterococcus faecium 300 mg, lactose 50 mg). Qualitative and quantitative changes of oral microbiota, concentration of immunoglobulins (sIgA, IgA, IgM) and cytokines (IL-6, IL-8, IL-1, IL-4, INF, TNF) in periodontal samples were recorded. The number of periodontopathogens and regional blood flow in the periodontium under conditions of prolonged confinement and hypokinesia were studied. Results. In comparison with the background period during the time of isolation, a quantitative growth of obligate periodontopathogens was observed in the subjects. This was accompanied by increased levels of immunoglobulins (IgM, IgA, sIgA) and pro-inflammatory cytokines (IL-1, IL-6, IL-8). There was an increase in blood flow in the arteriolo- venular part of the microcirculatory channel of periodontal tissues after leaving isolation. Subsequently, there was a tendency to optimize microbiocenosis through the use of probiotic and autoprobiotic agents. Along with this, there was a decrease of anti-inflammatory interleukin IL-4 practically to the initial values on the 18th day.

Effect of a new domestic remedy for fixation of removable dentures on oral mucosal immunity

BACKGROUND: The persisting high incidence of complications in removable acrylic dentures should convince the researchers to understand the consequences of denture use on the oral health and whole organism.
 AIM: To examine the peculiarities of the oral mucosal immunity in patients with partial removable acrylic dentures and to evaluate the efficacy of the application of Asepta Prodontal denture fixation agent.
 MATERIAL AND METHODS: Mucosal immunity was studied in 93 (24 men and 69 women) elderly patients (aged 61 74 years) with partial tooth loss who were divided into three study groups. Patients in the control group 1 (n=30) did not use any dentures. Group 2 (n=33) had partial removable acrylic dentures and from the first day of the adaptation period were offered to use the domestic denture fixation cream Asepta Parodontal (ZAO VERTEX, Saint Petersburg, Russia). Partial removable acrylic dentures were also made for group 3 (n=30), but their adaptation period was conducted without the application of domestic Asepta Parodontal denture fixation cream.
 RESULTS: On day 15 of the adaptation period in groups 2 and 3, inflammatory changes in the denture bed mucosa were revealed in 9.1% and 56.7% of the cases, respectively. On day 30 of dynamic observation in groups 2 and 3, inflammatory changes in the prosthetic bed mucosa were noted in 6.1% and 40.0% of the cases, respectively. The average numbers of visits to the doctor for denture correction in groups 2 and 3 were 1.33 and 2.76, respectively. A significant increase was found in the levels of pro-inflammatory cytokines on day 15 of the study in the saliva of patients with removable dentures, who used them without the denture fixation agent Asepta Parodontal, whereas patients using Asepta Parodontal showed an increase only in interleukins (IL) 6 and 8. Moreover, on day 30 of the study, no differences from the control group were observed in the concentration of pro-inflammatory cytokines compared with the group using domestic denture fixation cream, and in the group without using the cream, no decrease in the level of pro-inflammatory cytokines was noted. The concentration of RAIL and IL-10 increased in group 3 on both day 15 and 30, whereas no significant differences were observed in the group using the cream.
 CONCLUSION: Better prosthetic bed condition, decreased concentration of pro-inflammatory cytokines with a simultaneous elevation of secretory IgA in the oral cavity, testifying to the decrease in inflammation, and an increase in the immunity of the oral mucosa was noted in patients using Asepta Parodontal denture fixation agent.

Regional specification of oral mucosal immunity.

In this issue of Science Immunology, Barreto de Albuquerque et al. track immune responsiveness to the foodborne pathogen Listeria monocytogenes during oral infection. Their findings extend the notion of compartmentalized immunity within the gastrointestinal tract to the oral cavity and provide previously unkown insights into regional specialization of oral immunity.

Th17-driven immunity to oral candidiasis is dependent on the microbiome and can be triggered by mono-colonization with segmented filamentous bacteria.

Abstract The opportunistic infection oropharyngeal candidiasis (OPC; oral thrush) is a common oral infection among the immunocompromised. The oral cavity is a major entry portal to the body and oral health impacts several aspects of host immunity. While much is now known about microbiome dependent Th17 induction and protection against intestinal candidiasis and other Th17 driven responses, our understanding of the role of the microbiome in oral Th17 induction and oral antifungal immunity is rather rudimentary. We demonstrate a direct role for the microbiome in triggering protection against OPC. While wildtype (WT) mice raised under specific pathogen free (SPF) conditions are resistant to OPC, WT germ free (GF) mice succumbed to infection. Reconstitution of WT-GF mice with segmented filamentous bacteria (SFB) was sufficient to restore protection against OPC with associated increase in Th17 cells, neutrophils, and antimicrobial responses in the oral mucosa. Thus, SFB mono-colonization in WT-GF mice triggers oral mucosal Th17 induction to protect against oral thrush and reveals a role of the microbiome in promoting oral mucosal immunity. Supported by R37-DE022550

Total tooth loss and its treatment with removable dentures: features of the microbiota and mucosal immunity

The prevention of denture stomatitis is an important measure in the clinic of orthopedic dentistry, because at present, in spite of the availability of dentures on artificial supports, full removable dentures are still widely in demand. The study of oral microbiome and mucosal immunity in persons using full removable acrylic dentures was carried out, as it is important for scientifically substantiated development of ways to prevent denture stomatitis. Sixty-seven elderly patients with total tooth loss were examined and divided into 3 study groups. Patients in Group 1 (19 people) did not use any dentures. Patients in group 2 (25 people) had full removable acrylic dentures and from the first day of the adaptation period were offered to use the domestic denture fixation cream Acepta Parodontal. Patients of group 3 (23 people) were also made full removable acrylic dentures, but their adaptation period took place without the use of denture fixation cream. During the clinical-laboratory study it was established that the use of domestic Acepta Parodontal denture fixation cream in patients with full removable acrylic dentures during the adaptation period leads to a decrease in the level of inflammation in the oral cavity, contributes to an increase in mucosal immunity of its mucous membranes, as evidenced by the increased synthesis of secretory immunoglobulin A. At the same time, improvement of oral microbiota was noted, including reduction of detectability of Candida albicans as the main pathogen associated with denture stomatitis.

Natural SARS-CoV-2 Infection Affects Neutralizing Activity in Saliva of Vaccinees.

BackgroundSARS-CoV-2 transmission mainly occurs through exposure of the upper airway mucosa to infected secretions such as saliva, which are excreted by an infected person. Thus, oral mucosal immunity plays a central role in the prevention of and early defense against SARS-CoV-2 infection. Although virus-specific antibody response has been extensively investigated in blood samples of SARS-CoV-2-infected patients and vaccinees, local humoral immunity in the oral cavity and its relationship to systemic antibody levels needs to be further addressed.Material and MethodsWe fine-tuned a virus neutralization assay (vNTA) to measure the neutralizing activity (NA) of plasma and saliva samples from 20 SARS-CoV-2-infected (SI), 40 SARS-CoV-2-vaccinated (SV), and 28 SARS-CoV-2-vaccinated subjects with a history of infection (SIV) using the “wild type” SARS-CoV-2 lineage B.1 (EU) and the Delta (B.1.617.2) strains. To validate the vNTA results, the presence of neutralizing antibodies (NAbs) to the spike receptor binding domain (RBD) was evaluated with an ELISA assay.ResultsNA to SARS-CoV-2 lineage B.1 (EU) was present in plasma samples from all the tested subjects, with higher titers in SIV compared to both SI and SV. Conversely, NA was detected in saliva samples from 10.3% SV, 45% SI, and 92.6% SIV, with significantly lower titers in SV compared to both SI and SIV. The detection of NAbs in saliva reflected its reduced NA in SV.DiscussionThe difference in NA of plasma vs. saliva was confirmed in a vNTA where the SARS-CoV-2 B.1 and Delta strains were tested head-to-head, which also revealed a reduced NA of both specimens compared to the B.1 variant.ConclusionsThe administration of SARS-CoV-2 vaccines was associated with limited virus NA in the oral cavity, as measured in saliva and in comparison to plasma. This difference was more evident in vaccinees without a history of SARS-CoV-2 infection, possibly highlighting the importance of local exposure at the site of virus acquisition to effectively prevent the infection and block its spread. Nevertheless, the presence of immune escape mutations as possibly represented by the SARS-CoV-2 Delta variant negatively affects both local and systemic efficacy of NA associated with vaccination.

Oral mucosal Cryoglobulin [ICD-10-CM Diagnostic Code D89.9 Cryoglobulin], Oral Mucosal Herd Immunity and the Immune Herd Plots among Periodontal Disease Patients

Forty five patients were diagnosed by specialist dentist of the team as periodontal disease patients. Basically they were found to be chronic periodontitis and chronic gingivitis. The study male and female patient groups [25 males and 20 females] were subjected to the detection of oral mucosal cryoglobulin and tempts to use cryoglobulin as a marker for oral mucosal herd immunity among these patients. Oral mucosal cyoprotein solutions were separated and characterized as oral mucosal cryoglobulin [ICD-10-CM Diagnostic code, D89.9 Cryglobulin]. Mucosal cryocrit percentages were ranged from one to seven percent. Oral mucosal cryoglobulin concentrations were ranged between 0.13 up to 2 mg/dl. In chronic periodontitis patients and 0.15 up to1.96 mg/dl.in chronic gingivitis patients. The individuals Mucosal herd immunity constitutes three basic immune fractions; low, moderate and high cryoglobulin responders. The immune herd plot types were matched as Gaussian distribution plot types. The findings of oral mucosal cryoglobulin among these patients in clinically indicative concentrations for the potential role may be played in the pathogenesis of chronic gingivitis and chronic periodontitis as it may cause precipitates in the local blood vessels of gum and periodotium.

Severe symptoms predict salivary interleukin-6, interleukin-1β, and tumor necrosis factor-α levels in children and youth with obsessive-compulsive disorder

ObjectiveChildhood-onset obsessive-compulsive disorder (OCD) has been associated with immune dysregulation, including aberrant plasma inflammatory markers and increased rates of infectious and immune-mediated disorders. Saliva may provide a minimally-invasive tool for assessing oral mucosal immunity and inflammatory biomarkers in this population. The primary aim of this study was to compare salivary defense proteins and inflammatory mediators in saliva from children and youth with OCD and healthy controls, and evaluate their associations with measures of oral health and OCD phenotype. MethodsIn this cross-sectional observational study, saliva was collected from 41 children and youth with childhood-onset OCD and 46 healthy controls. Levels of lysozyme, α-amylase, secretory immunoglobulin A (sIgA), C-reactive protein (CRP), interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) were quantified by enzyme-linked immunosorbent assays or electrochemiluminescent-based immunoassays. ResultsAll analytes were detectable in saliva. When adjusting for salivary flow rate and total protein, multiple linear regression models including demographic variables, oral health measures, and OCD status explained a significant proportion of the variance in IL-6, IL-1β, and sIgA but not TNF-α, CRP, α-amylase, or lysozyme levels. Diagnosis of OCD was associated with significantly higher IL-6 (β = 0.403, p = 0.026), while severity of OCD was a significant predictor of increased cytokines (IL-6, β = 0.325, p = 0.009; IL-1β, β = 0.284, p = 0.020; TNF-α, β = 0.269, p = 0.036), but not other analytes. ConclusionThese data point to the feasibility of analyzing soluble immune mediators in the saliva in childhood-onset OCD, suggesting that pro-inflammatory cytokines are associated with OCD diagnosis and symptom severity. Further work is required to elucidate the factors contributing to this association and implications for clinical practice.