Optical Trapping Setup Research Articles

Micro-integrated crossed-beam optical dipole trap system with long-term alignment stability for mobile atomic quantum technologies

Quantum technologies extensively use laser light for state preparation, manipulation, and readout. For field applications, these systems must be robust and compact, driving the need for miniaturized and highly stable optical setups and system integration. In this work, we present a micro-integrated crossed-beam optical dipole trap setup, the µXODT, designed for trapping and cooling 87Rb. This fiber-coupled setup operates at 1064 nm wavelength with up to 2.5 W optical power and realizes a free-space crossed beam geometry. The µXODT precisely overlaps two focused beams (w0 ≈ 33 µm) at their waists in a 45° crossing angle, achieving a position difference of ≤3.4 µm and a 0.998 power ratio between both beams with long-term stability. We describe the design and assembly process in detail, along with optical and thermal tests with temperatures of up to 65 °C. The system’s volume of 25 ml represents a reduction of more than two orders of magnitude compared to typically used macroscopic setups while demonstrating exceptional mechanical robustness and thermal stability. The µXODT is integrated with an 87Rb 3D MOT setup, trapping 3 × 105 atoms from a laser-cooled atomic cloud, and has shown no signs of degradation after two years of operation.

Numerical and experimental demonstrations of optical trapping and manipulation of a selective red blood cell using a photonic hook based on broken symmetry tapered fiber probe

Optical tweezers and trapping technologies have a crucial impact on scientific research by precisely manipulating mesoscale objects at wavelength scales. The development of simple fiber-based optical tweezers foreseeing sorting and manipulation of a single cell has been firstly encouraged by biomedical requirements. The ability to manipulate mesoscale objects with high flexibility and precision is essential for a wide class of different fields. The ability to precisely arrange cells into desired structures is important for numerous biomedical and physical applications. This study experimentally reports for the first time a simple and low-cost strategy to produce an optical fiber-based photonic hook for achieving new unprecedented functionalities to selective particle manipulation in a lab-on-tip platform. We show perspectives opened by combining the fields of structured light in the form of the photonic hook and optical manipulation method. This enables new applications for optical trapping setups based on structured fields in the form of the photonic hook produced by an optical fiber tip with broken symmetry, where it becomes possible to manipulate and sort cells selectively.

Enhancement of optical levitation with hyperbolic metamaterials

The tightly focused laser beam in an optical trap has become a useful tool for many recent research areas. The momentum change in the photon-stream path of incident laser beam induces radiation force that enables trapping and manipulating mesoscopic micron-sized objects. In this study, we report the first analytical demonstration of optical trapping and levitation with radiation pressure on a transparent micron-sized spherical object made of hyperbolic metamaterial (HMM). The optical radial and axial forces acting on dielectric and HMM spherical particles are calculated using ray-optics approximation, assuming an optical levitation trapping setup. We compared the net force acting on the two objects, finding that the net radiation force exerted towards HMM particle is enhanced in the axial direction: The optical force enhancement in the HMM particle is more than ~ 8 times stronger compared to the induced force on the conventional dielectric particle with the corresponding material parameters. Besides, a better performance in the radial stabilization is observed for the HMM particle in comparison with the dielectric case, at which some oscillations and unstable saturation locations for the radial stabilization is monitored for TEM00 beam incidence. Furthermore, “zero-force” paths where radial stabilization of the HMM particle exists are also obtained for both TEM00 and TEM01∗\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$TEM_{01}^{*}$$\\end{document} laser beam incidences. Such phenomenon does not occur for particles of only dielectric and only metal material, which can be considered as another superiority of the proposed HMM particle.

Few-body hydrodynamic interactions probed by optical trap pulling experiment.

We study the hydrodynamic coupling of neighboring micro-beads placed in a multiple optical trap setup allowing us to precisely control the degree of coupling and directly measure time-dependent trajectories of entrained beads. We performed measurements on configurations with increasing complexity starting with a pair of entrained beads moving in one dimension, then in two dimensions, and finally a triplet of beads moving in two dimensions. The average experimental trajectories of a probe bead compare well with the theoretical computation, illustrating the role of viscous coupling and setting timescales for probe bead relaxation. The findings also provide direct experimental corroborations of hydrodynamic coupling at large, micrometer spatial scales and long, millisecond timescales, of relevance to, e.g., microfluidic device design and hydrodynamic-assisted colloidal assembly, improving the capability of optical tweezers, and understanding the coupling between micrometer-scale objects within a living cell.

Optical trapping and fluorescence control with vectorial structured light

Here we functionalized micro-scaled polymer beads with nano-scaled quantum dots and demonstrate optical trapping and tweezing, with in-situ fluorescence measurement, in an all-digital all-optical configuration. We outline the chemistry required to facilitate this, from deactivating the optical trapping environment to size, adhesion and agglomeration control. We introduce a novel holographic optical trapping set-up that leverages on vectorially structured light, allowing for the delivery of tuneable forms of light from purely scalar to purely vector, including propagation invariant flat-top beams for uniform illumination and tailored intensity gradient landscapes. Finally, we show how this has the potential to quench bleaching in a single wavelength trap by linear (spatial mode) rather than non-linear effects, advancing the nascent field of optics for chemistry.

Improved two-photon photopolymerisation and optical trapping with aberration-corrected structured light

We demonstrate the effectiveness of phase only aberration corrections of structured light and their application to versatile optical trapping setups. We calculate phase corrections before (ex-situ) and after (in-situ) a high numerical aperture microscope objective using a spatial light modulator (SLM), and investigate how these corrections can be used to improve the efficiency and resolution of micro-structures fabricated through two-photon-photopolymerisation (2PP). We apply a phase retrieval algorithm to correct for distortions in a femtosecond laser that enables the fabrication of 3D structures using as many as 50 simultaneous foci. The inclusion of aberration correction in the fabrication process shows improved confinement of optically trapped particles and more efficient polymerisation while minimising intensity variations at individual foci, which potentially damage the structure during fabrication. We find that phase corrections allow for consistent voxel sizes, increased sharpness, and an expanded effective printing range when using an SLM, while also allowing for closer proximity of individual trap foci, minimising interference effects that hinder fabrication resolution.

Optical pulling and pushing forces via Bloch surface waves

For flexible tailoring of optical forces, as well as for extraordinary optomechanical effects, additional degrees of freedom should be introduced into a system. Here, we demonstrate that photonic crystals are a versatile platform for optical manipulation due to both Bloch surface waves (BSWs) and the complex character of the reflection coefficient paving a way for controlled optomechanical interactions. We demonstrate enhanced pulling and pushing transversal optical forces acting on a single dipolar bead above a one-dimensional photonic crystal due to directional excitation of BSWs. Our results demonstrate angle- or wavelength-assisted switching between BSW-induced optical pulling and pushing forces. Easy to fabricate for any desired spectral range, photonic crystals are shown to be prospective for precise optical sorting of nanoparticles, which are difficult to sort with conventional optomechanical methods. Our approach opens opportunities for novel, to the best of our knowledge, optical manipulation schemes and platforms, and enhanced light-matter interaction in optical trapping setups.

All-optical matter-wave lens using time-averaged potentials

The precision of matter-wave sensors benefits from interrogating large-particle-number atomic ensembles at high cycle rates. Quantum-degenerate gases with their low effective temperatures allow for constraining systematic errors towards highest accuracy, but their production by evaporative cooling is costly with regard to both atom number and cycle rate. In this work, we report on the creation of cold matter-waves using a crossed optical dipole trap and shaping them by means of an all-optical matter-wave lens. We demonstrate the trade off between lowering the residual kinetic energy and increasing the atom number by reducing the duration of evaporative cooling and estimate the corresponding performance gain in matter-wave sensors. Our method is implemented using time-averaged optical potentials and hence easily applicable in optical dipole trapping setups.

Dynamic measurement of the acoustic streaming time constant utilizing an optical tweezer.

The combination of a bulk acoustic wave device and an optical trap allows for studying the buildup time of the respective acoustic forces. In particular, we are interested in the time it takes to build up the acoustic radiation force and acoustic streaming. For that, we measure the trajectory of a spherical particle in an acoustic field over time. The shape of the trajectory is determined by the acoustic radiation force and by acoustic streaming, both acting on different time scales. For that, we utilize the high temporal resolution (Δt=0.8μs) of an optical trapping setup. With our experimental parameters the acoustic radiation force on the particle and the acoustic streaming field theoretically have characteristic buildup times of 1.4μs and 1.44ms, respectively. By choosing a resonance mode and a measurement position where the acoustic radiation force and acoustic streaming induced viscous drag force act in orthogonal directions, we can measure the evolution of these effects separately. Our results show that the particle is accelerated nearly instantaneously by the acoustic radiation force to a constant velocity, whereas the acceleration phase to a constant velocity by the acoustic streaming field takes significantly longer. We find that the acceleration to a constant velocity induced by streaming takes in average about 17 500 excitation periods (≈4.4ms) longer to develop than the one induced by the acoustic radiation force. This duration is about four times larger than the so-called momentum diffusion time which is used to estimate the streaming buildup. In addition, this rather large difference in time can explain why a pulsed acoustic excitation can indeed prevent acoustic streaming as it has been shown in some previous experiments.

Localization, transport, and edge states in a two-strand ladder network in an aperiodically staggered magnetic field

We investigate the spectral and transport properties of a two-arm tight-binding ladder perturbed by an external magnetic field following an Aubry-Andr\'e-Harper profile. The varying magnetic flux trapped in consecutive ladder-cells simulates an axial twist that enables us, in principle, to probe a wide variety of systems ranging from a ribbon Hofstadter geometry to helical DNA chains. We perform an in-depth numerical analysis, using a direct diagonalization of the lattice Hamiltonian to study the electronic spectra and transport properties of the model. We show that such a geometry creates a self-similar multifractal pattern in the energy landscape. The spectral properties are analyzed using the local density of states and a Green's function formalism is employed to obtain the two-terminal transmission probability. With the standard multifractal analysis and the evaluation of inverse participation ratio we show that, the system hosts both critical and extended phase for a slowly varying aperiodic sequence of flux indicating a possible mobility edge. Finally, we report signatures of topological edge modes that are found to be robust against a correlated perturbation given to the nearest neighbor hopping integrals. Our results can be of importance in experiments involving ladder-like quantum networks, realized with cold atoms in an optical trap setup.

All-dielectric silicon metalens for two-dimensional particle manipulation in optical tweezers

Dynamic control of compact chip-scale contactless manipulation of particles for bioscience applications remains a challenging endeavor, which is restrained by the balance between trapping efficiency and scalable apparatus. Metasurfaces offer the implementation of feasible optical tweezers on a planar platform for shaping the exerted optical force by a microscale-integrated device. Here we design and experimentally demonstrate a highly efficient silicon-based metalens for two-dimensional optical trapping in the near-infrared. Our metalens concept is based on the Pancharatnam–Berry phase, which enables the device for polarization-sensitive particle manipulation. Our optical trapping setup is capable of adjusting the position of both the metasurface lens and the particle chamber freely in three directions, which offers great freedom for optical trap adjustment and alignment. Two-dimensional (2D) particle manipulation is done with a relatively low-numerical-aperture metalens ( NA ML = 0.6 ). We experimentally demonstrate both 2D polarization-sensitive drag and drop manipulation of polystyrene particles suspended in water and transfer of angular orbital momentum to these particles with a single tailored beam. Our work may open new possibilities for lab-on-a-chip optical trapping for bioscience applications and microscale to nanoscale optical tweezers.

Optical Micromachines for Biological Studies.

Optical tweezers have been used for biological studies since shortly after their inception. However, over the years research has suggested that the intense laser light used to create optical traps may damage the specimens being studied. This review aims to provide a brief overview of optical tweezers and the possible mechanisms for damage, and more importantly examines the role of optical micromachines as tools for biological studies. This review covers the achievements to date in the field of optical micromachines: improvements in the ability to produce micromachines, including multi-body microrobots; and design considerations for both optical microrobots and the optical trapping set-up used for controlling them are all discussed. The review focuses especially on the role of micromachines in biological research, and explores some of the potential that the technology has in this area.

RNA Folding and Unfolding Under Force: Single-Molecule Experiments and Their Analysis.

We have previously described (Geffroy et al. Methods Mol Biol 1665:25-40, 2018) how to unfold (or fold) a single RNA molecule under force using a dual-beam optical trap setup. In this chapter, we complementarily describe how to analyze the corresponding data and how to interpret it in terms of RNA three-dimensional structure. As with all single-molecule methods, single RNA molecule force data often exhibit several discrete states where state-to-state transitions are blurred in a noisy signal. In order to cope with this limitation, we have implemented a novel strategy to analyze the data, which uses a hidden Markov modeling procedure. A representative example of such an analysis is presented.

Realization of a high power optical trapping setup free from thermal lensing effects.

Transmission of high power laser beams through partially absorbing materials modifies the light propagation via a thermally-induced effect known as thermal lensing. This may cause changes in the beam waist position and degrade the beam quality. Here we characterize the effect of thermal lensing associated with the different elements typically employed in an optical trapping setup for cold atoms experiments. We find that the only relevant thermal lens is represented by the TeO2 crystal of the acousto-optic modulator exploited to adjust the laser power on the atomic sample. We then devise a simple and totally passive scheme that enables to realize an inexpensive optical trapping apparatus essentially free from thermal lensing effects.

All-fiber interferometer for displacement and velocity measurement of a levitated particle in fiber-optic traps.

We propose an all-fiber interferometer based on laser Doppler velocimetry in a dual-beam fiber-optic trap to measure the displacement and velocity of a trapped particle. ABCD matrices are used to compute the contrast ratio of the interference. The influence of the reflectivity of the fiber end face is discussed. We have designed an optimized reflectivity based on the parameters of our setup. The antireflective coatings on the fiber end face are employed to achieve the given reflectivity. The displacement and velocity of the trapped microparticle are successfully measured by the period and frequency of the interference signal, respectively. The sensitivity of the displacement detection is 368nm. By describing the miniaturization of the detection system, this paper provides a simple and practical scheme to achieve the integration of the entire optical trapping setup.

Experimental characterization and modeling of optical tweezer particle handling dynamics.

We report a new framework for a quantitative understanding of optical trapping (OT) particle handling dynamics. We present a novel three-dimensional particle-based model that includes optical, hydrodynamic, and inter-particle forces. This semi-empirical colloid model is based on an open-source simulation code known as LAMMPS (large-scale atomic/molecular massively parallel simulator) and properly recapitulates the full OT force profile beyond the typical linear approximations valid near the trap center. Simulations are carried out with typical system parameters relevant for our experimental holographic optical trapping (HOT) system, including varied particle sizes, trap movement speeds, and beam powers. Furthermore, we present a new experimental method for measuring both the stable and metastable boundaries of the optical force profile to inform or validate the model's underlying force profile. We show that our framework is a powerful tool for accurately predicting particle behavior in a practical experimental OT setup and can be used to characterize and predict particle handling dynamics within any arbitrary OT force profile.

Optical Manipulation along an Optical Axis with a Polarization Sensitive Meta-Lens.

The ability to manipulate small objects with focused laser beams opens a broad spectrum of opportunities in fundamental and applied studies, for which precise control over mechanical path and stability is required. Although conventional optical tweezers are based on refractive optics, the development of compact trapping devices that could be integrated within fluid cells is in high demand. Here, a plasmonic polarization-sensitive metasurface-based lens, embedded within a fluid, is demonstrated to provide several stable trapping centers along the optical axis. The position of a particle is controlled with the polarization of the incident light, interacting with plasmonic nanoscale patch antennas, organized within overlapping Fresnel zones of the lens. While standard diffractive optical elements face challenges in trapping objects in the axial direction outside the depth of focus, bifocal Fresnel meta-lens demonstrates the capability to manipulate a bead along a 4 μm line. An additional fluorescent module, incorporated within the optical trapping setup, was implemented and enabled the accurate mapping of optical potentials via a particle-tracking algorithm. Auxiliary micro- and nanostructures, integrated within fluidic devices, provide numerous opportunities to achieve flexible optomechanical manipulation, including transport, trapping, and sorting, which are in high demand for lab-on-a-chip applications and many others.

On the force–velocity relationship of a bundle of rigid bio-filaments

In various cellular processes, bio-filaments like F-actin and F-tubulin are able to exploit chemical energy associated with polymerization to perform mechanical work against an obstacle loaded with an external force. The force–velocity relationship quantitatively summarizes the nature of this process. By a stochastic dynamical model, we give, together with the evolution of a staggered bundle of Nf rigid living filaments facing a loaded wall, the corresponding force–velocity relationship. We compute the evolution of the model in the infinite wall diffusion limit and in supercritical conditions (monomer density reduced by critical density ρ^1>1), and we show that this solution remains valid for moderate non-zero values of the ratio between the wall diffusion and the chemical time scales. We consider two classical protocols: the bundle is opposed either to a constant load or to an optical trap setup, characterized by a harmonic restoring force. The constant load case leads, for each F value, to a stationary velocity Vstat(F;Nf,ρ^1) after a relaxation with characteristic time τmicro(F). When the bundle (initially taken as an assembly of filament seeds) is subjected to a harmonic restoring force (optical trap load), the bundle elongates and the load increases up to stalling over a characteristic time τOT. Extracted from this single experiment, the force–velocity VOT(F;Nf,ρ^1) curve is found to coincide with Vstat(F;Nf,ρ^1), except at low loads. We show that this result follows from the adiabatic separation between τmicro and τOT, i.e., τmicro ≪ τOT.

Fabrication of Fresnel plates on optical fibres by FIB milling for optical trapping, manipulation and detection of single cells

The development of economical optical devices with a reduced footprint foreseeing manipulation, sorting and detection of single cells and other micro particles have been encouraged by cellular biology requirements. Nonetheless, researchers are still ambitious for advances in this field. This paper presents Fresnel zone and phase plates fabricated on mode expanded optical fibres for optical trapping. The diffractive structures were fabricated using focused ion beam milling. The zone plates presented in this work have focal distance of ~5 µm, while the focal distance of the phase plates is ~10 µm. The phase plates are implemented in an optical trapping configuration, and 2D manipulation and detection of 8 µm PMMA beads and yeast cells is reported. This enables new applications for optical trapping setups based on diffractive optical elements on optical fibre tips, where feedback systems can be integrated to automatically detect, manipulate and sort cells.

Particle-Based Modeling of Living Actin Filaments in an Optical Trap.

We report a coarse-grained molecular dynamics simulation study of a bundle of parallel actin filaments under supercritical conditions pressing against a loaded mobile wall using a particle-based approach where each particle represents an actin unit. The filaments are grafted to a fixed wall at one end and are reactive at the other end, where they can perform single monomer (de)polymerization steps and push on a mobile obstacle. We simulate a reactive grand canonical ensemble in a box of fixed transverse area A, with a fixed number of grafted filaments , at temperature T and monomer chemical potential . For a single filament case () and for a bundle of filaments, we analyze the structural and dynamical properties at equilibrium where the external load compensates the average force exerted by the bundle. The dynamics of the bundle-moving-wall unit are characteristic of an over-damped Brownian oscillator in agreement with recent in vitro experiments by an optical trap setup. We analyze the influence of the pressing wall on the kinetic rates of (de)polymerization events for the filaments. Both static and dynamic results compare reasonably well with recent theoretical treatments of the same system. Thus, we consider the proposed model as a good tool to investigate the properties of a bundle of living filaments.