Abstract
The development of economical optical devices with a reduced footprint foreseeing manipulation, sorting and detection of single cells and other micro particles have been encouraged by cellular biology requirements. Nonetheless, researchers are still ambitious for advances in this field. This paper presents Fresnel zone and phase plates fabricated on mode expanded optical fibres for optical trapping. The diffractive structures were fabricated using focused ion beam milling. The zone plates presented in this work have focal distance of ~5 µm, while the focal distance of the phase plates is ~10 µm. The phase plates are implemented in an optical trapping configuration, and 2D manipulation and detection of 8 µm PMMA beads and yeast cells is reported. This enables new applications for optical trapping setups based on diffractive optical elements on optical fibre tips, where feedback systems can be integrated to automatically detect, manipulate and sort cells.
Highlights
Light driven tools, such as, optical tweezers (OTs), are one of the main breakthroughs of the last decades
This paper presents a set of Fresnel zone and phase plates, fabricated on common optical fibres using focused ion beam milling for trapping purposes
The measured values were: 33% and 38% for the Fresnel zone plate (FZP)-1 and 2 and 60% and 67% for Fresnel phase plate (FPP)-1 and 2, respectively. This proves that the losses are higher for the case of the zone plates, since the light is blocked by alternated zones, while the phase plates are more efficient in the conversion of the optical profiles
Summary
The studies of the optical properties of the zone and phase plates presented in the previous sections demonstrated some important features that differentiates both Fresnel diffractive structures. In contrast to the zone plates, the phase plates only originate one focal point, the central peak is wider in the transversal direction. Longitudinal optical output beam profile for FZP-2: (c) experimental; (d) computational. Transversal optical output beam profile at the main focal point: (e) FZP-1; (f) FZP-2. This proves that the losses are higher for the case of the zone plates, since the light is blocked by alternated zones, while the phase plates are more efficient in the conversion of the optical profiles. Longitudinal optical output beam profile for FPP-2: (c) experimental; (d) computational. Transversal optical output beam profile at the main focal point: (e) FPP-1; (f) FPP-2
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