Optical Nanotweezers Research Articles

Optical Trapping and Fast Discrimination of Label-Free Bacteriophages at the Single Virion Level.

There is a recent resurgence of interest in phage therapy (the therapeutic use of bacterial viruses) as an approach to eliminating difficult-to-treat infections. However, existing approaches for therapeutic phage selection and virulence testing are time-consuming, host-dependent, and facing reproducibility issues. Here, this study presents an innovative approach wherein integrated resonant photonic crystal (PhC) cavities in silicon are used as optical nanotweezers for probing and manipulating single bacteria and single virions with low optical power. This study demonstrates that these nanocavities differentiate between a bacterium and a phage without labeling or specific surface bioreceptors. Furthermore, by tailoring the spatial extent of the resonant optical mode in the low-index medium, phage distinction across phenotypically distinct phage families is demonstrated. The work paves the road to the implementation of optical nanotweezers in phage therapy protocols.

Optical Monitoring of In Situ Iron Loading into Single, Native Ferritin Proteins.

Ferritin is a protein that stores and releases iron to prevent diseases associated with iron dysregulation in plants, animals, and bacteria. The conversion between iron-loaded holo-ferritin and empty apo-ferritin is an important process for iron regulation. To date, studies of ferritin have used either ensemble measurements to quantify the characteristics of a large number of proteins or single-molecule approaches to interrogate labeled or modified proteins. Here we demonstrate the first real-time study of the dynamics of iron ion loading and biomineralization within a single, unlabeled ferritin protein. Using optical nanotweezers, we trapped single apo- and holo-ferritins indefinitely, distinguished one from the other, and monitored their structural dynamics in real time. The study presented here deepens the understanding of the iron uptake mechanism of ferritin proteins, which may lead to new therapeutics for iron-related diseases.

In-plane subwavelength optical capsule for lab-on-a-chip nano-tweezers.

In this Letter, we propose a new, to the best of our knowledge, proof-of-concept of optical nano-tweezers based on a pair of dielectric rectangular structures that are capable of generating a finite-volume in-plane optical capsule. Finite-difference time-domain simulations of light spatial distributions and optical trapping forces of a gold nanoparticle immersed in water demonstrate the physical concept of an in-plane subwavelength optical capsule integrated with a microfluidic mesoscale device. It is shown that the refractive index of and the distance between the two dielectric rectangular structures can effectively control the shape and axial position of the optical capsule. Such an in-plane mesoscale structure provides a new path for manipulating absorbing nano-particles or bio-particles in a compact planar architecture, and should thus lead to promising perspectives in lab-on-a-chip domains.

High‐Quality Plasmonic Materials TiN and ZnO:Al by Atomic Layer Deposition

Electromagnetic radiation when coupled to collective oscillations of free electrons, dubbed as plasmonics, makes it possible to manipulate light at dimensions well below the diffraction limit and substantially enhances light–matter interaction. Plasmonics has already enabled many novel technologies with a wide variety of application in chemical and biosensing, medical treatments, nonlinear and quantum optics, metamaterials, optical nanotweezers, nanolasers, solar cells, light‐emitting diodes, and telecommunications. Coating the well‐established semiconductor circuitry with metals, such as Au and Ag, imparts the stack with much coveted plasmonic properties, but the metals suffer from high dissipative losses, limited optical tunability, and poor mechanical, chemical, and thermal stabilities, which render them undesirable. Emerging alternative plasmonic materials, such as TiN and ZnO:Al, overcome these limitations and offer wide tunability of their electrical and optical properties. Among a wide range of techniques used for the preparation of TiN and ZnO:Al thin films, atomic layer deposition (ALD) offers advantages such as conformity, scalability, and low growth temperature, which makes this technique the most suitable for the integration of plasmonics with the complementary metal–oxide–semiconductor (CMOS) electronics. Herein, a brief review of recent advances in ALD‐grown TiN and ZnO:Al thin films as pertained to plasmonic applications is given.

Exploring the Limit of Multiplexed Near-Field Optical Trapping

Optical trapping has revolutionized our understanding of biology by manipulating cells and single molecules using optical forces. Moving to the near-field creates intense field gradients to trap very smaller particles, such as DNA fragments, viruses, and vesicles. The next frontier for such optical nanotweezers in biomedical applications is to trap multiple particles and to study their heterogeneity. To this end, we have studied dielectric metasurfaces that allow the parallel trapping of multiple particles. We have explored the requirements for such metasurfaces and introduce a structure that allows the trapping of a large number of nanoscale particles (>1000) with a very low total power P < 26 mW. We experimentally demonstrate the near-field enhancement provided by the metasurface and simulate its trapping performance. We have optimized the metasurface for the trapping of 100 nm diameter particles, which will open up opportunities for new biological studies on viruses and extracellular vesicles, such as studying heterogeneity, or to massively parallelize analyses for drug discovery.

Plasmonic optical trapping of nanoparticles using T-shaped copper nanoantennas.

We demonstrate the optical trapping of single dielectric nanoparticles in a microfluidic chamber using a coupled T-shaped copper plasmonic nanoantenna for studying light-matter interaction. The nanoantenna is composed of two identical copper elements separated by a 50 nm gap and each element is designed with two nanoblocks. Our nanoantenna inherits three different advantages compared to previous plasmonic nanoantennas, which are usually made of gold. First, copper is a very promising plasmonic material with its very similar optical properties as gold. Second, copper is comparably cheap, which is compatible with industry-standard fabrication processes and has been widely used in microelectronics. Third, the trapping area of tweezers is expanded due to the intrinsic Fabry-Perot cavity with two parallel surfaces. We present finite element method simulations of the near-field distribution and photothermal effects. And we perform Maxwell stress tensor simulations of optical forces exerted on an individual nanoparticle in the vicinity of the nanoantenna. In addition, we examine how the existence of an oxide layer of cupric oxide and the heat sink substrate influence the optical trapping properties of copper nanoantennas. This work demonstrates that the coupled T-shaped copper nanoantennas are a promising means as optical nanotweezers to trap single nanoparticles in solution, opening up a new route for nanophotonic devices in optical information processing and on-chip biological sensing.

Efficient nano-tweezers via a silver plasmonic bowtie notch with curved grooves

Localized surface plasmon resonance (LSPR) with a sharp field gradient and extremely strong field intensity is very attractive for near-field optical nano-tweezers manipulating tiny bio-objects with sizes ranging from sub-10 nm to micrometers. To lower the power consumption of optical trapping and improve the accessibility of LSPR for the targets, we propose and study a plasmonic bowtie notch design with assisted periodic curved grooves. By generating and coupling extra surface plasmon polariton waves into the LSPR, the presented design herein can significantly reduce the required power consumption in experiments for stably trapping single and multiple polystyrene spheres. We believe the idea and results presented in this paper would be helpful in realizing highly efficient nano-tweezers in the plasmonic circuit on-chip.

Next-Generation Optical Nanotweezers for Dynamic Manipulation: From Surface to Bulk.

Optical traps based on strongly confined electromagnetic fields at metal-dielectric interfaces are far more efficient than conventional optical tweezers. Specifically, these near-field nanotweezers allow the trapping of smaller particles at lower optical intensities, which can impact diverse research fields ranging from soft condensed matter physics to materials science and biology. A major thrust in the past decade has been focused on extending the capabilities of plasmonically enhanced nanotweezers beyond diffusion-limited trapping on surfaces such as to achieve dynamic control in the bulk of fluidic environments. Here, we review the recent efforts in optical nanotweezers, especially those involving hybrid forcing schemes, covering both surface and bulk-based techniques. We summarize the important capabilities demonstrated with this promising approach, with niche applications in reconfigurable nanopatterning and on-chip assembly as well as in sorting and separating colloidal nanoparticles.

Algorithmic approach for designing plasmonic nanotweezers.

We use machine learning (simulated annealing) to design plasmonic nanoapertures that function as optical nanotweezers. The nanoapertures have irregular shapes that are chosen by our algorithm. We present electromagnetic simulations that show that these produce stronger field enhancements and extraction energies than nanoapertures comprising double nanoholes with the same gap geometry. We show that performance is further improved by etching one or more rings into the gold surrounding the nanoaperture. Lastly, we provide a direct comparison between our design and work that is representative of the state of the art in plasmonic nanotweezers at the time of writing.

Low-loss plasmon-assisted electro-optic modulator.

For nearly two decades, the field of plasmonics1 - which studies the coupling of electromagnetic waves to the motion of free electrons in a metal2 - has sought to realize subwavelength optical devices for information technology3–6, sensing7,8, nonlinear optics9,10, optical nanotweezers11 and biomedical applications12. Although the heat generated by ohmic losses is desired for some applications (e.g. photo-thermal therapy), plasmonic devices for sensing and information technology have largely suffered from these losses inherent to metals13. This has led to a widespread stereotype that plasmonics is simply too lossy to be practical. Here, we demonstrate that these losses can be bypassed by employing “resonant switching”. In the proposed approach, light is only coupled to the lossy surface plasmon polaritons in the device’s off-state (in resonance) where attenuation is desired to ensure large extinction ratios and facilitate sub-ps switching. In the on state (out of resonance), light is prevented from coupling to the lossy plasmonic section by destructive interference. To validate the approach, we fabricated a plasmonic electro-optic ring modulator. The experiments confirm that low on-chip optical losses (2.5 dB), high-speed operation (>>100 GHz), good energy efficiency (12 fJ/bit), low thermal drift (4‰ K-1), and a compact footprint (sub-λ radius of 1 μm) can be realized within a single device. Our result illustrates the potential of plasmonics to render fast and compact on-chip sensing and communications technologies.

Optical Manipulation of nanoparticles by simultaneous electric and magnetic field enhancement within diabolo nanoantenna

In this paper, we propose and numerically simulate a novel optical trapping process based on the enhancement and the confinement of both magnetic and electric near-fields by using gold Diabolo Antenna (DA). The later was recently proposed to generate huge magnetic near-field when illuminated by linearly polarized wave along its axis. Numerical 3D – FDTD simulation results demonstrate the high confinement of the electromagnetic field in the vicinity of the DA. This enhancement is then exploited for the trapping of nano-particles (NP) as small as 30 nm radius. Results show that the trapping process greatly depends on the particle dimensions and that three different regimes of, trapping at contact, trapping without contact, or pushing can be achieved within the same DA. This doubly resonant structure opens the way to the design of a novel generation of efficient optical nano-tweezers that allow manipulation of nano-particles by simply changing the operation wavelength.

Design of a high-performance optical tweezer for nanoparticle trapping

Integrated optical nanotweezers offer a novel paradigm for optical trapping, as their ability to confine light at the nanoscale leads to extremely high gradient forces. To date, nanotweezers have been realized either as photonic crystal or as plasmonic nanocavities. Here, we propose a nanotweezer device based on a hybrid photonic/plasmonic cavity with the goal of achieving a very high quality factor-to-mode volume (Q/V) ratio. The structure includes a 1D photonic crystal dielectric cavity vertically coupled to a bowtie nanoantenna. A very high Q/V ~ 107 (λ/n)−3 with a resonance transmission T = 29 % at λ R = 1381.1 nm has been calculated by 3D finite element method, affording strong light–matter interaction and making the hybrid cavity suitable for optical trapping. A maximum optical force F = −4.4 pN, high values of stability S = 30 and optical stiffness k = 90 pN/nm W have been obtained with an input power P in = 1 mW, for a polystyrene nanoparticle with a diameter of 40 nm. This performance confirms the high efficiency of the optical nanotweezer and its potential for trapping living matter at the nanoscale, such as viruses, proteins and small bacteria.

Molecular weight characterization of single globular proteins using optical nanotweezers.

We trap a set of molecular weight standard globular proteins using a double nanohole optical trap. The root mean squared variation of the trapping laser transmission intensity gives a linear dependence with the molecular weight, showing the potential for analysis of globular proteins. The characteristic time of the autocorrelation of the trapping laser intensity variations scales with a -2/3 power dependence with the volume of the particle. A hydrodynamic laser tweezer model is used to explain these dependencies. Since this is a single particle technique that operates in solution and can be used to isolate an individual particle, we believe that it provides an interesting alternative to existing analysis methods and shows promise to expand the capabilities of protein related studies to the single particle level.

Three-dimensional manipulation with scanning near-field optical nanotweezers

Recent advances in nanotechnologies have prompted the need for tools to accurately and non-invasively manipulate individual nano-objects. Among the possible strategies, optical forces have been predicted to provide researchers with nano-optical tweezers capable of trapping a specimen and moving it in three dimensions. In practice, however, the combination of weak optical forces and photothermal issues has thus far prevented their experimental realization. Here, we demonstrate the first three-dimensional optical manipulation of single 50nm dielectric objects with near-field nanotweezers. The nano-optical trap is built by engineering a bowtie plasmonic aperture at the extremity of a tapered metal-coated optical fibre. Both the trapping operation and monitoring are performed through the optical fibre, making these nanotweezers totally autonomous and free of bulky optical elements. The achieved trapping performances allow for the trapped specimen to be moved over tens of micrometres over a period of several minutes with very low in-trap intensities. This non-invasive approach is foreseen to open new horizons in nanosciences by offering an unprecedented level of control of nanosized objects, including heat-sensitive biospecimens.

Enhanced Optical Trapping and Arrangement of Nano-Objects in a Plasmonic Nanocavity

Gentle manipulation of micrometer-sized dielectric objects with optical forces has found many applications in both life and physical sciences. To further extend optical trapping toward the true nanometer scale, we present an original approach combining self-induced back action (SIBA) trapping with the latest advances in nanoscale plasmon engineering. The designed resonant trap, formed by a rectangular plasmonic nanopore, is successfully tested on 22 nm polystyrene beads, showing both single- and double-bead trapping events. The mechanism responsible for the higher stability of the double-bead trapping is discussed, in light of the statistical analysis of the experimental data and numerical calculations. Furthermore, we propose a figure of merit that we use to quantify the achieved trapping efficiency and compare it to prior optical nanotweezers. Our approach may open new routes toward ultra-accurate immobilization and arrangement of nanoscale objects, such as biomolecules.