Purpose: The purpose of this study is to evaluate the existing team dynamics within the surgical crew; to gain insights into the perceptions of different team members regarding team effectiveness and potential areas for improvement; to investigate factors crucial to successful teamwork in the operation theatre, and to analyze successful practices that have positively impacted team performance and patient outcomes. Design/methodology/approach: Quantitative data was gathered through a validated questionnaire distributed among surgical team members at a private cancer hospital and research center in Thrissur District, Kerala. Descriptive research was adapted for this study. The population for the study includes the members of the surgical team. A sample size of 120 members including doctors, nurses, technicians, and other supportive members who participated in various surgeries, were selected using a random sampling technique for the data purpose. Findings: The findings of the study disclosed that the challenges included occasional communication breakdowns and role ambiguity that seriously influenced the team performance of a surgical crew. Successful practices involved structured communication protocols, regular team training, and a collaborative leadership approach. Stakeholders accentuated the need for continuous improvement in strategies premeditated to the operation theatre’s unique demands. Research limitations/implications: The study’s inferences underscore the necessity for targeted intermediations focusing on the factors manipulating the surgical teams. Implementing structured training programs, refining communication protocols, and fostering a collaborative leadership culture can notably improve team performance. Additionally, modifying these interventions to suit the specific demands of the operation theatre is essential for sustaining and augmenting positive changes in teamwork dynamics, ultimately elevating the quality of patient care and outcomes. Social Implications: The empirical research contributed to enhancing the quality of surgical care, improving patient safety, and refining the overall performance of surgical teams in specialized healthcare settings through enhanced team performance for societal well-being by improving patient outcomes and reducing healthcare disparities. A more cohesive surgical team fosters a safer environment, positively impacting patient satisfaction, trust in healthcare, and overall community health. Additionally, it promotes a culture of collaboration and excellence, setting a precedent for improved healthcare practices within society. Originality / Value: The originality of the study lies in its broad examination of team dynamics within a specialized setting, the operation theatre of a private cancer hospital, and a research center. The study offers insights into specific challenges, successful practices, and context-specific interventions, to enhance team performance and patient care in this distinct healthcare environment.