Open State Research Articles

Research on dust control technology and numerical simulation of conical guiding air curtain in fully mechanized excavation face

The dust pollution caused by the operation of fully mechanized heading face poses a serious threat to the safety production of operators and working face. To reduce dust concentration at the fully mechanized heading face, this study analyzed dust samples collected from various positions to understand the particle size distribution characteristics. Based on these findings, a conical diversion air conditioning (CDAC) device was designed to create a radial air curtain for dust control in the roadway cross-section. Computational Fluid Dynamics (CFD) was then employed to investigate the airflow and particle dynamics when the cone-shaped deflector was in closed and open states. The results show that in the fully mechanized heading face, the dust distribution in the working area of the roadheader driver is relatively dense, and the dust particles with particle size ≤ 8 μm account for a large proportion. When the CDAC device is deployed, the axial airflow in the roadway is changed into a rotating airflow along the roadway wall, and an air screen is established in the working area of the roadheader driver to block the outward diffusion of dust. When the pressure air outlet is arranged 30 m away from the tunneling head, the pressure air volume is set to 400 m3/min, and the CDAC device can better form the air curtain barrier to block the dust particles. It provides a new method for effectively controlling the dust concentration of the fully mechanized heading face and directly ensuring the health of the roadheader driver.

Targeting SHP2 Cryptic Allosteric Sites for Effective Cancer Therapy.

SHP2, a pivotal component downstream of both receptor and non-receptor tyrosine kinases, has been underscored in the progression of various human cancers and neurodevelopmental disorders. Allosteric inhibitors have been proposed to regulate its autoinhibition. However, oncogenic mutations, such as E76K, convert SHP2 into its open state, wherein the catalytic cleft becomes fully exposed to its ligands. This study elucidates the dynamic properties of SHP2 structures across different states, with a focus on the effects of oncogenic mutation on two known binding sites of allosteric inhibitors. Through extensive modeling and simulations, we further identified an alternative allosteric binding pocket in solution structures. Additional analysis provides insights into the dynamics and stability of the potential site. In addition, multi-tier screening was deployed to identify potential binders targeting the potential site. Our efforts to identify a new allosteric site contribute to community-wide initiatives developing therapies using multiple allosteric inhibitors to target distinct pockets on SHP2, in the hope of potentially inhibiting or slowing tumor growth associated with SHP2.

Pore-Forming VDAC Proteins of the Outer Mitochondrial Membrane: Regulation and Pathophysiological Role.

Voltage-dependent anion channels (VDAC1-3) of the outer mitochondrial membrane are a family of pore-forming β-barrel proteins that carry out controlled "filtration" of small molecules and ions between the cytoplasm and mitochondria. Due to the conformational transitions between the closed and open states and interaction with cytoplasmic and mitochondrial proteins, VDACs not only regulate the mitochondrial membrane permeability for major metabolites and ions, but also participate in the control of essential intracellular processes and pathological conditions. This review discusses novel data on the molecular structure, regulatory mechanisms, and pathophysiological role of VDAC proteins, as well as future directions in this area of research.

Dynamic molecular portraits of ion-conducting pores characterize functional states of TRPV channels

Structural biology is solving an ever-increasing number of snapshots of ion channel conformational ensembles. Deciphering ion channel mechanisms, however, requires understanding the ensemble dynamics beyond the static structures. Here, we present a molecular modeling-based approach characterizing the ion channel structural intermediates, or their “dynamic molecular portraits”, by assessing water and ion conductivity along with the detailed evaluation of pore hydrophobicity and residue packing. We illustrate the power of this approach by analyzing structures of few vanilloid-subfamily transient receptor potential (TRPV) channels. Based on the pore architecture, there are three major states that are common for TRPVs, which we call α-closed, π-closed, and π-open. We show that the pore hydrophobicity and residue packing for the open state is most favorable for the pore conductance. On the contrary, the α-closed state is the most hydrophobic and always non-conducting. Our approach can also be used for structural and functional classification of ion channels.

Dynamics underlie the drug recognition mechanism by the efflux transporter EmrE

The multidrug efflux transporter EmrE from Escherichia coli requires anionic residues in the substrate binding pocket for coupling drug transport with the proton motive force. Here, we show how protonation of a single membrane embedded glutamate residue (Glu14) within the homodimer of EmrE modulates the structure and dynamics in an allosteric manner using NMR spectroscopy. The structure of EmrE in the Glu14 protonated state displays a partially occluded conformation that is inaccessible for drug binding by the presence of aromatic residues in the binding pocket. Deprotonation of a single Glu14 residue in one monomer induces an equilibrium shift toward the open state by altering its side chain position and that of a nearby tryptophan residue. This structural change promotes an open conformation that facilitates drug binding through a conformational selection mechanism and increases the binding affinity by approximately 2000-fold. The prevalence of proton-coupled exchange in efflux systems suggests a mechanism that may be shared in other antiporters where acid/base chemistry modulates access of drugs to the substrate binding pocket.

Evaluating Hydrogeomorphic Condition Across Ecosystem States in a Non-tidal, Brackish Peat Marsh of the Florida Coastal Everglades, USA

Emergent marsh and open water have been identified as alternate stable states in tidal marshes with large, relative differences in hydrogeomorphic conditions. In the Florida coastal Everglades, concern has been raised regarding the loss of non-tidal, coastal peat marsh via dieback of emergent vegetation and peat collapse. To aid in the identification of alternate stable states, our objective was to characterize the variability of hydrogeomorphic and biologic conditions using a field survey and long-term monitoring of hydrologic and geomorphic conditions across a range of vegetated (emergent, submerged) and unvegetated (open water) communities, which we refer to as “ecosystem states,” in a non-tidal, brackish peat marsh of the coastal Everglades. Results show (1) linear relationships among field-surveyed geomorphic, hydrologic, and biologic variables, with a 35-cm mean difference in soil surface elevation between emergent and open water states, (2) an overall decline in soil elevation in the submerged state that was related to cumulative dry days, and (3) a 2× increase in porewater salinity during the dry season in the emergent state that was also related to the number of dry days. Coupled with findings from previous experiments, we propose a conceptual model that describes how seasonal hydrologic variability may lead to ecosystem state transitions between emergent and open water alternate states. Since vegetative states are only moderately salt tolerant, as sea-level rise pushes the saltwater front inland, the importance of continued progress on Everglades restoration projects, with an aim to increase the volume of freshwater being delivered to coastal wetlands, is the primary management intervention available to mitigate salinization and slow ecosystem state shifts in non-tidal, brackish peat marshes.

Photoinhibition of the hERG potassium channel PAS domain by ultraviolet light speeds channel closing

hERG potassium channels are critical for cardiac excitability. hERG channels have a Per-Arnt-Sim (PAS) domain at their N-terminus, and here, we examined the mechanism for PAS domain regulation of channel opening and closing (gating). We used TAG codon suppression to incorporate the noncanonical amino acid 4-benzoyl-L-phenylalanine (BZF), which is capable of forming covalent cross-links after photoactivation by ultraviolet (UV) light, at three locations (G47, F48, and E50) in the PAS domain. We found that hERG-G47BZF channels had faster closing (deactivation) when irradiated in the open state (at 0 mV) but showed no measurable changes when irradiated in the closed state (at −100 mV). hERG-F48BZF channels had slower activation, faster deactivation, and a marked rightward shift in the voltage dependence of activation when irradiated in the open (at 0 mV) or closed (at −100 mV) state. hERG-E50BZF channels had no measurable changes when irradiated in the open state (at 0 mV) but had slower activation, faster deactivation, and a rightward shift in the voltage dependence of activation when irradiated in the closed state (at −100mV), indicating that hERG-E50BZF had a state-dependent difference in UV photoactivation, which we interpret to mean that PAS underwent molecular motions between the open and closed states. Moreover, we propose that UV-dependent biophysical changes in hERG-G47BZF, F48BZF, and E50BZF were the direct result of photochemical cross-linking that reduced dynamic motions in the PAS domain and broadly stabilized the closed state relative to the open state of the channel.

Closed cracks characterization in a steel sample applying a pump probe waves ultrasonic method

Abstract Ultrasonic measurements are commonly used for crack sizing in industrial context, but due to potential partial crack closure, the crack depth can be underestimated. It could be of importance regarding the prediction of remaining service life and maintenance scheduling of industrial components. In the literature, nonlinear ultrasonic methods have proven efficient for this issue. Among them, a pump probe waves method is investigated here for possible use in an industrial context. In this paper, an industrial steel component with several partially closed fatigue cracks is studied. First, a three-point bending test associated with digital image correlation is performed with ultrasonic measurements to obtain reliable indications about the cracks closure state. A three-dimensional laser vibrometry experiment shows the possibility to open the crack using a pump wave. The pump probe waves method is conducted using a standard 45° shear wave transducer in echo mode as a probe. The results allow to discuss physical mechanism in play. It is also shown the possibility to detect the closed nature for all of the cracks even if the full opening state is not reached. Moreover, a coherent crack profile is found compared to mechanical test. The results are validated on a notched sample for which the nonlinear response is negligible. Industrial application as well as possible improvement are discussed.

Parallel photocycle kinetic model of anion channelrhodopsin GtACR1 function

The light-gated anion channelrhodopsin GtACR1 is an important optogenetic tool for neuronal silencing. Its photochemistry, including its photointermediates, is poorly understood. The current mechanistic view presumes BR-like kinetics and assigns the open channel to a blue-absorbing L intermediate. Based on time-resolved absorption and electrophysiological data, we recently proposed a red-absorbing spectral form for the open channel state. Here, we report the results of a comprehensive kinetic analysis of the spectroscopic data combined with channel current information. The time evolutions of the spectral forms derived from the spectroscopic data are inconsistent with the single chain mechanism and are analyzed within the concept of parallel photocycles. The spectral forms partitioned into conductive and nonconductive parallel cycles are assigned to intermediate states. Rejecting reversible connections between conductive and nonconductive channel states leads to kinetic schemes with two independent conductive states corresponding to the fast- and slow-decaying current components. The conductive cycle is discussed in terms of a single cycle and two parallel cycles. The reaction mechanisms and reaction rates for the wild-type protein, the A75E, and the low-conductance D234N and S97E protein variants are derived. The parallel cycles of channelrhodopsin kinetics, its relation to BR photocycle, and the role of the M intermediate in channel closure are discussed.

Navigating the paradox: Exploring resident experiences of vulnerability.

Learning and growth in postgraduate medical education (PGME) often require vulnerability, defined as a state of openness to uncertainty, risk, and emotional exposure. However, vulnerability can threaten a resident's credibility and professional identity. Despite this tension, studies examining vulnerability in PGME are limited. As such, this study aims to explore residents' experiences of vulnerability, including the factors that influence vulnerability in PGME. Using a constructivist grounded theory approach, individual semi-structured interviews were conducted with 15 residents from 10 different specialities. Interview transcripts were coded and analysed iteratively. Themes were identified and relationships among themes were examined to develop a theory describing vulnerability in PGME. Residents characterised vulnerability as a paradox represented by two overarching themes. 'Experiencing the tensions of vulnerability' explores the polarities between being a fallible, authentic learner and an infallible, competent professional. 'Navigating the vulnerability paradox' outlines the factors influencing the experience of vulnerability and its associated outcomes at the intrapersonal, interpersonal, and systems levels. Residents described needing to have the bandwidth to face the risks and emotional labour of vulnerability. Opportunities to build connections with social agents, including clinical teachers and peers, facilitated vulnerability. The sociocultural context shaped both the experience and outcomes of vulnerability as residents faced the symbolic mask of professionalism. Residents experience vulnerability as a paradox shaped by intrapersonal, interpersonal, and systems level factors. These findings capture the nuance and complexity of vulnerability in PGME and offer insight into creating supportive learning environments that leverage the benefits of vulnerability while acknowledging its risks. There is a need to translate this understanding into systems-based change to create supportive PGME environments, which value and celebrate vulnerability.

Molecular Mechanism of Phosphorylation-Mediated Impacts on the Conformation Dynamics of GTP-Bound KRAS Probed by GaMD Trajectory-Based Deep Learning.

The phosphorylation of different sites produces a significant effect on the conformational dynamics of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations were combined with deep learning (DL) to explore the molecular mechanism of the phosphorylation-mediated effect on conformational dynamics of the GTP-bound KRAS. The DL finds that the switch domains are involved in obvious differences in conformation contacts and suggests that the switch domains play a key role in the function of KRAS. The analyses of free energy landscapes (FELs) reveal that the phosphorylation of pY32, pY64, and pY137 leads to more disordered states of the switch domains than the wild-type (WT) KRAS and induces conformational transformations between the closed and open states. The results from principal component analysis (PCA) indicate that principal motions PC1 and PC2 are responsible for the closed and open states of the phosphorylated KRAS. Interaction networks were analyzed and the results verify that the phosphorylation alters interactions of GTP and magnesium ion Mg2+ with the switch domains. It is concluded that the phosphorylation pY32, pY64, and pY137 tune the activity of KRAS through changing conformational dynamics and interactions of the switch domains. We anticipated that this work could provide theoretical aids for deeply understanding the function of KRAS.

The three-dimensional structure of DapE from Enterococcus faecium reveals new insights into DapE/ArgE subfamily ligand specificity

DapE is a Zn2+-metallohydrolase recognized as a drug target for bacterial control. It is a homodimer that requires the exchange of interface strands by an induced fit essential for catalysis. Identifying novel anti-DapE agents requires greater structural details. Most of the characterized DapEs are from the Gram-negative group. Here, two high-resolution DapE crystal structures from Enterococcus faecium are presented for the first time with novel aspects. A loosened enzyme intermediate between the open and closed conformations is observed. Substrates may bind to loose state, subsequently it closes, where hydrolysis occurs, and finally, the change to the open state leads to the release of the products. Mutation of His352 suggests a role, along with His194, in the oxyanion stabilization in the mono-metalated Zn2+ isoform, while in the di-metalated isoform, the metal center 2 complements it function. An aromatic-π box potentially involved in the interaction of DapE with other proteins, and a peptide flip could determine the specificity in the Gram-positive ArgE/DapE group. Finally, details of two extra-catalytic cavities whose geometry changes depending on the conformational state of the enzyme are presented. These cavities could be a target for developing non-competitive agents that trap the enzyme in an inactive state.

Some mechanistic underpinnings of molecular adaptations of SARS-COV-2 spike protein by integrating candidate adaptive polymorphisms with protein dynamics.

We integrate evolutionary predictions based on the neutral theory of molecular evolution with protein dynamics to generate mechanistic insight into the molecular adaptations of the SARS-COV-2 spike (S) protein. With this approach, we first identified candidate adaptive polymorphisms (CAPs) of the SARS-CoV-2 S protein and assessed the impact of these CAPs through dynamics analysis. Not only have we found that CAPs frequently overlap with well-known functional sites, but also, using several different dynamics-based metrics, we reveal the critical allosteric interplay between SARS-CoV-2 CAPs and the S protein binding sites with the human ACE2 (hACE2) protein. CAPs interact far differently with the hACE2 binding site residues in the open conformation of the S protein compared to the closed form. In particular, the CAP sites control the dynamics of binding residues in the open state, suggesting an allosteric control of hACE2 binding. We also explored the characteristic mutations of different SARS-CoV-2 strains to find dynamic hallmarks and potential effects of future mutations. Our analyses reveal that Delta strain-specific variants have non-additive (i.e., epistatic) interactions with CAP sites, whereas the less pathogenic Omicron strains have mostly additive mutations. Finally, our dynamics-based analysis suggests that the novel mutations observed in the Omicron strain epistatically interact with the CAP sites to help escape antibody binding.

Some mechanistic underpinnings of molecular adaptations of SARS-COV-2 spike protein by integrating candidate adaptive polymorphisms with protein dynamics

We integrate evolutionary predictions based on the neutral theory of molecular evolution with protein dynamics to generate mechanistic insight into the molecular adaptations of the SARS-COV-2 spike (S) protein. With this approach, we first identified candidate adaptive polymorphisms (CAPs) of the SARS-CoV-2 S protein and assessed the impact of these CAPs through dynamics analysis. Not only have we found that CAPs frequently overlap with well-known functional sites, but also, using several different dynamics-based metrics, we reveal the critical allosteric interplay between SARS-CoV-2 CAPs and the S protein binding sites with the human ACE2 (hACE2) protein. CAPs interact far differently with the hACE2 binding site residues in the open conformation of the S protein compared to the closed form. In particular, the CAP sites control the dynamics of binding residues in the open state, suggesting an allosteric control of hACE2 binding. We also explored the characteristic mutations of different SARS-CoV-2 strains to find dynamic hallmarks and potential effects of future mutations. Our analyses reveal that Delta strain-specific variants have non-additive (i.e., epistatic) interactions with CAP sites, whereas the less pathogenic Omicron strains have mostly additive mutations. Finally, our dynamics-based analysis suggests that the novel mutations observed in the Omicron strain epistatically interact with the CAP sites to help escape antibody binding.

Three-dimensional architecture of ESCRT-III flat spirals on the membrane

The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and cytokinetic abscission. ESCRT-III, the most abundant ESCRT subunit, assembles into flat spirals as the primed state, essential to initiate membrane invagination. However, the three-dimensional architecture of ESCRT-III flat spirals remained vague for decades due to highly curved filaments with a small diameter and a single preferred orientation on the membrane. Here, we unveiled that yeast Snf7, a component of ESCRT-III, forms flat spirals on the lipid monolayers using cryogenic electron microscopy. We developed a geometry-constrained Euler angle-assigned reconstruction strategy and obtained moderate-resolution structures of Snf7 flat spirals with varying curvatures. Our analyses showed that Snf7 subunits recline on the membrane with N-terminal motifs α0 as anchors, adopt an open state with fused α2/3 helices, and bend α2/3 gradually from the outer to inner parts of flat spirals. In all, we provide the orientation and conformations of ESCRT-III flat spirals on the membrane and unveil the underlying assembly mechanism, which will serve as the initial step in understanding how ESCRTs drive membrane abscission.

Repeated clearing as a mechanism for savanna recovery following bush encroachment

Abstract Many savannas are experiencing increased cover of trees and shrubs, resulting in reduced herbaceous productivity, shifts in savanna functional structure and potential reductions in ecotourism. Clearing woody plants has been suggested as an effective management strategy to mitigate these effects and restore these systems to an open state with higher rates of grass production and herbivory. This study investigated the effectiveness of repeated shrub clearing as a tool to mitigate bush encroachment in a semi‐arid savanna in southern Africa. We present data from a 7‐year experiment in the Mthimkhulu Game Reserve bordering Kruger National Park, South Africa. Colophospermum mopane stems and resprouting shoots were basally cut 2–3 times per year (2015–2022) in three pairs of treatment and control plots of 60 × 60 m. We monitored changes in soil moisture, grass biomass and herbivore activity via dung counts. We assessed C. mopane physiological responses to repeated cutting using non‐structural carbohydrates and stable water isotopes to infer changes to energy storage and functional rooting depth, respectively. The cleared treatment had higher soil moisture and grass biomass than the control treatment. Dung counts showed impala and buffalo visited the cleared treatment more frequently than the control treatment. Repeated cutting had limited effects on C. mopane survival in the first 2–3 years after initial clearing, but 80% of individuals were dead after 7 years. Repeatedly cut C. mopane had lower belowground starch concentrations and used water from shallower soil depths than C. mopane in control plots. Synthesis and applications. Repeated cutting increased soil moisture availability and grass biomass, and attracted charismatic grazing herbivores. While more costly than once‐off clearing methods, this practice created more employment opportunities for a neighbouring rural community. Transforming portions of the ecosystem to a grass‐dominated state may increase ecotourism potential through improved game viewing in open systems.

Quantitative EEG Microstates Predict Correct Responses During Visuo-Spatial Working Memory Task

Hypothesis: EEG microstate maps during pre-response period of Visuo-Spatial Working Memory (VSWM) Task can predict correct or incorrect response in healthy population and patients with schizophrenia. Methods: After taking informed consent, 30 patients diagnosed with schizophrenia (as per DSM-5 criteria) and 30 controls (both age and gender matched) were recruited. Thereafter, 128 channel EEG was recorded during eyes closed and eyes open states as well as during VSWM task performance. The VSWM task entails matching pairs of identical multicolored abstract pictures hidden in arrays of different cognitive loads after an initial encoding. Following the acquisition; EEG pre-processing, down sampling, average referencing, and Independent Component Analysis were done. Epoch of 100 milliseconds just before each response was taken and grouped into either correct or incorrect trials following which they were subjected to microstate analysis. Statistical analysis was done to compare temporal microstate parameters (Global explained variance, mean duration, time coverage, and segment count density) of each microstate map found between correct and incorrect trials. Data and Results: Microstate maps of pre-response state during correct and incorrect trials were compared in patients with schizophrenia and controls taken together as well as separately. Global explained variance of Microstate Map A (RA-LP, Right Anterior- Left Posterior orientation) was found to be significantly more during pre-response period when the responses were correct as compared to incorrect trials. GEV of Map A emerged out to be the key predictor of correct response in all three groups: overall Correct versus incorrect trials (patients and controls taken together), among patients with schizophrenia, and among controls. Conclusions: Global Explained Variance of EEG microstates Map A is a good predictor of correct response in VSWM task. Department of Physiology, All India Institute of Medical Sciences, New Delhi. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Offline Fault Diagnosis for 2-Level Inverter: Short-Circuit and Open-Circuit Detection

Fault detection is very important to improve the reliability of power conversion devices. Faults of power semiconductors can be broadly divided into shorts and opens and are further classified into two types depending on whether there is an internal problem with the switch or anti-parallel diode. In this paper, fault-diagnosis methods for short-circuit and open-circuit states are proposed, respectively. A method of classifying and diagnosing faults by applying a gate signal to each switch is proposed to diagnose short-circuit conditions. This method uses only current magnitude information, which reduces the amount of required information and reduces diagnostic failures due to angle errors and current noise. A method is proposed to detect a faulty switch by applying a voltage vector and comparing the current angle with a lookup table to diagnose an open state. An iterative diagnostic algorithm is proposed to prevent diagnostic failure due to angle error and current noise. The effectiveness of the proposed diagnosis method is verified through experiments and simulations.

Numerical Investigation of Micrometer-Sensitive Particle Intrusion in Hydraulic Valve Clearances and Its Impact on Valve Performance

The intrusion of micrometer-sensitive contaminant particles into the clearance of sliding valves within hydraulic fluids is one of the root causes of valve sticking and reliability issues in hydraulic systems. To reveal the transient process and characteristics of particle intrusion into the clearance process, this paper proposes a numerical method for fluid–particle one-way coupling and verifies it through experimentation. Furthermore, a numerical simulation of the motion trajectory of spherical iron particles inside the valve chamber was conducted in a two-dimensional flow model. It was discovered that in a steady-state flow field with a certain valve opening, micrometer-sized particles in the valve chamber’s hydraulic fluid mainly move with the valve flow stream, and the number of micron particles invading the slide valve clearance and the probability of invasion is related to the slide valve opening and differential pressure. When the slide valve opening decreases, especially in the small opening state, the probability of particles invading the slide valve clearance will increase dramatically, and the probability of invading the clearance is as high as 27% in a valve opening of 50 μm; the larger the pressure difference between the valve ports, the more the number of particles invading the slide valve clearance increases; the particles in the inlet of the slide valve clearance are more prone to invade the slide valve clearance, and invade in an inclined way, touching the wall and then bouncing back. These findings are of great value for the design of highly reliable hydraulic control valves and the understanding of the mechanism of slide valve stalls and provide an important scientific basis for the optimization and improvement in the reliability of hydraulic systems.

Research and development of innovative bidirectional control plate valve for reciprocating compressor

Currently, the reciprocating compressor valve operates on a single working principle, where the valve structure relies on the combined force of its own elasticity and gas thrust to drive the movement of the valve disc, and continues to open and close in the way of impact. Due to the defects of the conventional plate valve, such as delayed opening, premature closing, and insufficient full opening time, in combination with the self-acting valve structure and constant volume cylinder where artificial adjustment of compressed air volume is not feasible. Therefore an innovative bidirectional control plate valve has been developed in this study, which employs a linear motor to regulate the valve disc and eliminates structural components such as springs, buffer discs, and valve lift guard. Throughout the entire opening and closing cycle, precise control of the valve disc enables swift operation with prolonged full open state, minimal resistance loss, and absence of impact, vibration or rebound. Its ideal movement mode can directly and accurately achieve stepless capacity regulation. The primary suction valve of the industrial 3LW-5-8 reciprocating compressor is taken as the research subject in this paper. Fluent software is utilized to design the structural parameters of an innovative plate valve, and its feasibility and effectiveness are validated through tests conducted on an automated multi-functional experiment table and an L-type reciprocating compressor. The results demonstrate that the new valve exhibits a 21.52% increase in effective flow area compared to the conventional plate valve, along with a 3.02% increase in exhaust volume and a potential reduction of 2.32% in power consumption when replaced one of the four. Moreover, the innovative bidirectional control plate valve demonstrates precise control over high-frequency motion of the valve disc, effectively addressing inherent limitations of traditional plate valves and exhibiting promising application prospects.