Understanding offshore site amplification (AF) characteristics is crucial for marine engineering. Thus far, the spectral ratio (SR) has been widely used to analyze offshore AF characteristics, despite its reliability not yet being fully investigated. In this study, we used 826 ground-motion data recorded at 6 offshore and 19 onshore stations in the Sagami Bay region to reveal the source, path, and AF characteristics using the generalized inversion technique (GIT). The average stress drops for the shallow crustal, subduction interface, and subduction slab earthquakes were 3.55, 6.59, and 7.07 MPa, respectively. The offshore sites were categorized into flat and steep stations based on the local slope gradient (LSG), and systematic AF characteristics were observed. We found that the topography had a negligible influence on the flat free-field station KNG205. AFs obtained using the GIT and SR were analyzed and compared, revealing two main differences: (1) SR-obtained AF values were significantly larger than GIT-obtained AF values above 0.5 Hz and 5 Hz at the flat and steep sites, respectively; (2) resonant frequencies estimated using the SR drifted to lower and higher frequencies for flat and steep sites, respectively. The ineffectiveness of the SR for flat and steep stations was attributed to the effects of topography and seawater pressure on low- and high-frequency vertical ground-motions, respectively. These results suggest that the SR is only reliable for estimating AF curves at marine free-field sites and low-frequency AFs at steep sites. The LSG may play an important role in offshore ground-motion studies.