Purpose: Patients with an anterior cruciate ligament (ACL) injury are at high risk of developing OA. ACL injury and knee osteoarthritis (OA) are both associated with impaired sensory function. Sensory function is commonly assessed as knee kinesthesia using laboratory equipment. To enable evaluation of larger groups of subjects, and evaluation outside the laboratory, portable equipment would be preferable. Vibration sense, assessed with portable equipment, is used as a measure of sensory function in subjects with OA, but is sparsely studied in subjects with ACL injury. A sufficiently high correlation between kinesthesia and vibration sense would indicate that one measurement can replace the other. The purpose of this investigation was to study the association between kinesthesia and vibration sense to elucidate whether these measurements could be used interchangeably. In addition, the associations between kinesthesia, vibration sense and functional performance and patient-reported outcomes, respectively, were assessed. Methods: Fifty three patients with ACL injury (mean age 24 years, range 18–35) and 46 matched controls (mean age 26 years, range 18–35) were included. Sensory function was assessed two ways: 1) by the threshold to detection of passive motion (TDPM) for knee kinesthesia; and 2) by vibration perception threshold (VPT) at the medial malleolus and the medial femoral condyle for vibration sense. As a measure of motor function, the one leg hop test for distance was used. The Knee injury and Osteoarthritis Outcome Score (KOOS) and the Tegner Activity Scale (TAS) were used as measures of self-reported outcomes and activity level, respectively. For the comparisons, Pearson’s correlation coefficient (r) and Spearman rank correlation coefficient (rs) were used as appropriate. Results: A low correlation was found between TDPM and VPT at the medial malleolus in patients (r = 0.208, p = 0.139) and controls (r = −0.279, p = 0.060). No correlation was found between TDPM and VPT at the femoral condyle in patients (r = 0.009, p = 0.950) or controls (r = −0.180, p = 0 .231). No relation was found between the sensory measures and the one-leg hop test in the patient group (r = 0.116–0.136, p = 0.41–0.48). There were no or low correlations between the sensory measures and one-leg hop test in the control group (r = 0.013–0.302, p = 0.12–0.95). No or low correlations were found between the three measures of sensory function and the KOOS subscales (r = 0.00–0.29, p = 0.04–0.98) and the TAS (rs = −0.05–0.35, p = 0.02–0.75), respectively. Conclusions: The low, or lack of, correlation between TDPM and VPT suggest that these measures of sensory function cannot be used interchangeably in subjects with ACL injury or in their control counterparts. These measurements were weakly related to both hop performance, activity level and the patient’s perceived symptoms, function and activities implying that both knee kinesthesia and vibration sense only affect motor performance, activity level and patients’ perception of function to a limited degree.