We consider a class of one-dimensional reaction-diffusion systems,\[\left\{\begin{array}[u_{t}=\varepsilon^{2}u_{xx}+f(u,w)\\\tau w_{t}=Dw_{xx}+g(u,w)\end{array}\right.\]with homogeneous Neumann boundary conditions on a one dimensional interval.Under some generic conditions on the nonlinearities $f,g$ and in the singularlimit $\varepsilon\rightarrow0,$ such a system admits a steady state for which$u$ consists of sharp back-to-back interfaces. For a sufficiently large $D$and for sufficiently small $\tau$, such a steady state is known to be stablein time. On the other hand, it is also known that in the so-called shadowlimit $D\rightarrow\infty,$ patterns having more than one interface areunstable. In this paper we analyse in detail the transition between the stablepatterns when $D=O(1)$ and the shadow system when $D\rightarrow\infty$. Weshow that this transition occurs when $D$ is exponentially large in $\varepsilon$ and we derive instability thresholds $D_{1}\gg D_{2}\ggD_{3}\gg\ldots$ such that a periodic pattern with $2K$ interfaces is stable if$D D_{K}$. We also study the dynamics of theinterfaces when $D$ is exponentially large; this allows us to describe indetail the mechanism leading to the instability. Direct numerical computationsof stability and dynamics are performed, and these results are in excellentagreement with corresponding results as predicted by the asymptotic theory.