On-line Procedure Research Articles

Structural predication and antigenic analysis of Toxoplasma gondii ROP20.

Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii. In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online procedure and Visual Molecular Dynamics (VMD) software. The structure analysis fully indicated that ROP20 protein is an important member of the ROP family. Furthermore, We used DNASTAR software and Epitope Database online service to analyze liner-B cell epitopes and T-cell epitopes of ROP20 protein. All the analysis results of ROP20 protein can provide positive information on treatment and vaccine for toxoplasmosis. Moreover, ROP20 gene was obtained from PCR, and a recombinant eukaryotic expression vector (pEGFP-C1-ROP20) was constructed in the following study. After restriction enzyme digestion, the constructed plasmid was transfected into HEK 293-T cells. The RT-PCR result indicated that the recombinant plasmid could transcribe successfully in HEK 293-T cell. The results of western blotting indicated the expressed proteins can be recognized by anti-STAg mouse sera.

A Framework for On-line Calibration of LINAC Devices

General description of an on-line procedure of calibration for IGRT (Image Guided Radiotherapy) is given. The algorithm allows to improve targeting cancer by estimating its position in space and suggests appropriate correction of the position of the patient. The description is given in the Geometric Algebra language which significantly simplifies calculations and clarifies presentation.

Capillary zone electrophoresis determination of fluoride in seawater using transient isotachophoresis.

We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of fluoride (F-) in seawater using transient isotachophoresis (tITP) as an on-line concentration procedure. A method of correcting sample salinity effects was also proposed so that F- concentrations were obtained using a calibration graph. The proposed method is simple: it requires no sample pretreatment aside from dilution. The following optimum conditions were established: background electrolyte (BGE), 5mM 2,6-pyridinedicarboxylic acid (PDC) adjusted to pH 3.5 containing 0.03% m/v hydroxypropyl methylcellulose (HPMC); detection wavelength, 200nm; vacuum (50kPa) injection period of sample, 5s (254nL); and applied voltage, 23kV with the sample inlet side as the cathode. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) for F- reached 0.024 and 0.070mg/L, respectively. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for F- were 2.5, 3.4, and 0.30%. The proposed method was applied for the determination of F- in seawater samples collected from coastal waters of western Japan during August 26-28, 2014. Both results obtained using standard addition method and a calibration graph agreed with those obtained using a conventional spectrophotometric method.

Analysis of E-mail Transactions in Virtual Reference Services

Today, the use of traditional reference desk in the academic libraries has been rarely used, thus expanding or even move to a virtual reference service. A minimum level of virtual reference services are provided in the academic library is currently in general is the electronic mail (e-mail). One of the academic library specifically provide virtual reference services via e-mail is a Petra Christian University (PCU) Library (refdesk@petra.ac.id.). In such services librarians provide assistance to users in finding information and answer questions. This study aimed to analyze the transaction reference services virtually through e-mail at the PCU Library, with a view of the types of questions based on user background, the writing style of language communication interaction used based on user background, and cultural values are revealed behind the user in virtual reference services (e-mail). This study uses content analysis (content analysis) of the transcript e-mail received librarians of reference services began March 10 until June 16, 2015. The results showed that the types of questions asked in reference service virtual (e-mail) in the Library UK Petra include: specific search, access online resources, operation of online resources, policies and procedures for services, and library holdings with background the student (PCU and non-PCU), faculty, and librarians. Based on the background of users found that overall more types of questions asked in virtual reference services (e-mail) is a problem of access to online resources, and generally submitted by the students. Then, the writing style of the user's language in interaction reference service virtual (e-mail) tends to be formal, which includes the word greeting, the message will be delivered, and regards cover, either by the student (PCU and non-PCU), lecturer, or librarians. While cultural values that revealed the background behind the user in virtual reference services (e-mail) is obedience, courtesy and politeness users. Keywords: virtual reference services, e-mail reference service, e-mail transactions, content analysis, academic libraries

Policy Document on Library Inventory of Books, Library Overdue Fines, Thesis Collection and Online Resources Database at Higher Quality Accredited Institutions

This Policy Document keeps up a refreshed stock of the considerable number of books as a major aspect of the library property. The motivation behind this strategy report and methodology is to set up a proficient library stock to limit/wipe out misfortunes in the library accumulation. This strategy archive covers just every one of the books as a component of the library property. This Policy Document additionally guarantees that online databases assets are remotely accessible to the grounds. This strategy record and system advances the utilization of the electronic assets accessible in help to understudies and employees for their learning procedures, educating and inquire about. This arrangement record covers the online database assets, for example, electronic magazines/diaries, digital books, procedures, modified works, references and different references. This Policy Document additionally guarantees that all books and other library assets are returned on schedule and to force fines for past due books and different materials. This approach report is given for appropriate checking of books in the dissemination and save segments of the library and guarantees that these are returned on schedule. This approach archive covers all understudies/personnel/staff who get/s book/s from the University library. This approach record and method means to compose the gathering and set up constant upgrade of the examination yields by the undergrad and graduate understudies for research purposes. This approach archive covers all the proposal submitted both by the undergrad and graduate understudies.

The Use of Telemedicine in Surgical Care: a Systematic Review.

Introduction:Telemedicine describes a healthcare service where physicians communicate with patients remotely using telecommunication technologies. Telemedicine is being used to provide pre-/postoperative surgical consultation and monitoring as well as surgical education.Aim:Our purpose was to investigate the broad range of telemedicine technologies used in surgical care.Material and methods:MEDLINE, EMBASE, CINAHL, and Science Direct were searched for available literature from inception to March 30, 2018 with no language restrictions. The search terms included: cell phones, telemedicine, telecommunications, video, online, videoconferencing, remote consultation, surgery, preoperative, perioperative, postoperative, and surgical procedures. Studies were included if they used telemedicine in surgery for pre-, peri-, or post-surgery periods, and if they compared traditional surgical care with surgical telemedicine. We excluded case series, case reports, and conference abstracts from our review.Results:A total of 24 studies were included in our review. The study found that the use of telemedicine in preoperative assessment and diagnosis, evaluation after surgery and follow-up visits to be beneficial. Patients reported benefits to using telemedicine such as avoiding unnecessary trips to hospitals, saving time and reducing the number of working days missed.Conclusion:Telemedicine in surgical care can provide benefits to both patients and clinicians.

The Acceptability of Online Consent in a Self-Test Serosurvey of Responders to the 2014-2016 West African Ebola Outbreak.

Online participation in research is used increasingly to recruit geographically dispersed populations. Obtaining online consent is convenient, yet we know little about the acceptability of this practice. We carried out a serostudy among personnel returning to the UK/Ireland following deployment to West Africa during the 2014–2016 Ebola epidemic. We used an online procedure for consenting returnees and designed a small descriptive study to understand: how much of the consent material they read, how informed they felt and if they preferred online to traditional face-to-face consent. Of 261 returnees, 111 (43 per cent) completed the consent survey. Participants indicated a high level of engagement with the consent materials, with 67 per cent reporting having read all and 20 per cent having read ‘most’ of the materials. All participants indicated feeling completely (78 per cent) or mostly (22 per cent) informed about the purpose, methods and intended uses of the research, as well as what participation was required and what risks were involved. Only three participants indicated a preference for face-to-face consent. Free-text comments suggested that online consent may be an acceptable modality for uncomplicated and low-risk studies. The study sample was largely composed of health professionals, suggesting acceptability of online consent within this population.

Toward Calibration-Free Continuous Glucose Monitoring Sensors: Bayesian Calibration Approach Applied to Next-Generation Dexcom Technology.

Continuous glucose monitoring (CGM) sensors need to be calibrated twice/day by using self-monitoring of blood glucose (SMBG) samples. Recently, to reduce the calibration frequency, we developed an online calibration algorithm based on a multiple-day model of sensor time variability and Bayesian parameter estimation. When applied to Dexcom G4 Platinum (DG4P) sensor data, the algorithm allowed the frequency of calibrations to be reduced to one-every-four-days without significant worsening of sensor accuracy. The aim of this study is to assess the same methodology on raw CGM data acquired by a next-generation Dexcom CGM sensor prototype and compare the results with that obtained on DG4P. The database consists of 55 diabetic subjects monitored for 10 days by a next-generation Dexcom CGM sensor prototype. The new calibration algorithm is assessed, retrospectively, by simulating an online procedure using progressively fewer SMBG samples until zero. Accuracy is evaluated with mean absolute relative differences (MARD) between blood glucose versus CGM values. The one-per-day and one-every-two-days calibration scenarios in the next-generation CGM data have an accuracy of 8.5% MARD (vs. 11.59% of DG4P) and 8.4% MARD (vs. 11.63% of DG4P), respectively. Accuracy slightly worsens to 9.2% (vs. 11.62% of DG4P) when calibrations are reduced to one-every-four-days. The calibration-free scenario results in 9.3% MARD (vs. 12.97% of DG4P). In next-generation Dexcom CGM sensor data, the use of an online calibration algorithm based on a multiple-day model of sensor time variability and Bayesian parameter estimation aids in the shift toward a calibration-free scenario with even better results than those obtained in present-generation sensors.

Vibration Sensor Monitoring of Nickel-Titanium Alloy Turning for Machinability Evaluation.

Nickel-Titanium (Ni-Ti) alloys are very difficult-to-machine materials causing notable manufacturing problems due to their unique mechanical properties, including superelasticity, high ductility, and severe strain-hardening. In this framework, the aim of this paper is to assess the machinability of Ni-Ti alloys with reference to turning processes in order to realize a reliable and robust in-process identification of machinability conditions. An on-line sensor monitoring procedure based on the acquisition of vibration signals was implemented during the experimental turning tests. The detected vibration sensorial data were processed through an advanced signal processing method in time-frequency domain based on wavelet packet transform (WPT). The extracted sensorial features were used to construct WPT pattern feature vectors to send as input to suitably configured neural networks (NNs) for cognitive pattern recognition in order to evaluate the correlation between input sensorial information and output machinability conditions.

Model Reference Adaptive Control based on a Self-Recurrent Wavelet Neural Network Utilizing Micro Artificial Immune Systems

Abstract 
 This paper presents an intelligent model reference adaptive control (MRAC) utilizing a self-recurrent wavelet neural network (SRWNN) to control nonlinear systems. The proposed SRWNN is an improved version of a previously reported wavelet neural network (WNN). In particular, this improvement was achieved by adopting two modifications to the original WNN structure. These modifications include, firstly, the utilization of a specific initialization phase to improve the convergence to the optimal weight values, and secondly, the inclusion of self-feedback weights to the wavelons of the wavelet layer. Furthermore, an on-line training procedure was proposed to enhance the control performance of the SRWNN-based MRAC. As the training method, the recently developed modified micro artificial immune system (MMAIS) was used to optimize the parameters of the SRWNN. The effectiveness of this control approach was demonstrated by controlling several nonlinear dynamical systems. For each of these systems, several evaluation tests were conducted, including control performance tests, robustness tests, and generalization tests. From these tests, the SRWNN-based MRAC has exhibited its effectiveness regarding accurate control, disturbance rejection, and generalization ability. In addition, a comparative study was made with other related controllers, namely the original WNN, the artificial neural network (ANN), and the modified recurrent network (MRN). The results of these comparison tests indicated the superiority of the SRWNN controller over the other related controllers.
 Keywords: Artificial neural network, micro artificial immune system, model reference adaptive control, self-recurrent wavelet neural network , Wavelet neural network.

Prioritization of non-recommended clinical activities in Primary Care

To prioritize non-recommended clinical activities in Primary Care (PC), from "Do not do" recommendations listed by the Sociedad Española de Medicina de Familia y Comunitaria (Semfyc), according to expert consensus (physicians, nurses and pharmacists). The consensus for the prioritization of non-recommended practices in PC was performed through an online procedure. We used as a base the list of "do not-do" recommendations of the SEMFYC. We asked the experts to prioritize practices that should be de-adopted in PC, based on four prioritization criteria: frequency of occurrence, cost of the activity, ease of disposal and damage caused, which were scored from one to five, according to their recommendation. Scores were summarized in median and quartile values. Two rounds were necessary to obtain a consensus. A modified e-Delphi technique was used. 34 experts (62%) participated in the first consultation round and prioritized 19 recommendations with a score = 3.5. These recommendations were again analyzed in a second round, in which 32 panelists agreed to prioritize 17 practices (13 related to prescription, three diagnostic tests, and one clinical analysis). The high priority list included seven practices with values = 4: 1) Prescription of a new drug in elderly patients without having reviewed the previous treatments; 2) Lipid-lowering drugs without calculating the overall cardiovascular risk; 3) Not systematically prescribing gastric protection with proton pump inhibitors to patients consuming Nonsteroidal anti-inflammatory drugs (NSAIDs); 4) Glucose self-analysis in non-insulinized type 2 diabetics; 5) Benzodiazepines in the long term; 6) Bisphosphonates in patients with low risk of fracture; and 7) Antibiotics in lower respiratory tract infections. This study provides information for the prioritization of 17 non-AP activities in which short-term de-adoption would significantly increase the efficiency of the public health system.

1. MR-guided adaptive stereotactic radiotherapy: A new paradigm in radiotherapy

Introduction In May 2016 we have implemented stereotactic MR-guided adaptive radiation therapy at the VU University Medical Center by using the MRIdian system (ViewRay, Inc.). Our approach consists of: (1) MR-guided tumor setup; (2) daily online plan adaptation to account for inter-fractional changes; (3) patient-specific QA; (4) gated treatment delivery. The key points of the MR-guided adaptive workflow and the potential for better OAR sparing and delivery accuracy are shown. Methods For each adaptive fraction a high-resolution MR acquisition for delineation and plan adaptation is taken. Scantime ranges from 17 s. to 150 s. according to protocol. Online plan (re-)optimization is done by keeping the same beam parameters as for the reference baselineplan. Before treatment delivery online patient-specific QA evaluation of the adapted plan is performed with a 3%/3 mm gamma comparison with the dose distribution from the TPS before accepting the adapted treatment plan for delivery. MR-guidance of gated treatments during delivery is performed in a single sagittal plan on the GTV with a safety margin of GTV + 3 mm. Results Well over 700 adaptive fractions in 130 patients under MR-guidance have been delivered to date. Treatment, generally delivered as hypofractionated SBRT included lesions in the pancreas, prostate, lung and liver. Plan adaptation was performed in a median time of 12 min and resulted nearly always in plans meeting the constraints of the clinical protocol. Online patient-specific QA was performed on average within 1.5 min resulting in average gamma pass-rate above 99% and gamma average value of 0.35. Plan (re-)optimization results in better sparing of OARs while ensuring adequate coverage to the PTV. Median duration of treatments was 50 min and it was delivered with video assisted feedback to the patient with a monitor installed in the treatment room. Conclusions An online procedure for plan adaptation and delivery under MR-guidance was successfully implemented at our institution, resulting in median times of 12 min. for plan adaptation and 50 min. for treatment duration. A very good agreement in the patient-specific QA was obtained for all adapted fractions.

Direct determination of δ44/42 Ca isotope ratio by ion chromatography/low-resolution multicollector ICPMS.

High-precision on-line procedure for measurement of calcium isotopic ratio by coupling ion chromatography to multicollector inductively coupled plasma mass spectrometry was developed. Calcium separation from the sample matrix was achieved on an ion chromatography column-IonPac CS16-ID 3mm connected with CERS 500 2mm suppressor and followed by multicollector inductively coupled plasma mass spectrometry calcium isotopic ratio determination. Dry plasma mode was used with Aridus II desolvation system. To sustained samples with high level of total dissolved salts as well as account capacity of applied analytical column, the method has been optimized regarding calcium isotope ratio measurements with low-resolution mass spectrometry. Mass discrimination and instrument drift were corrected by sample-standard bracketing method using the 44 Ca/42 Ca isotope ratio of SRM 915a as a standard. Good accuracy and reasonable precision of calcium isotope ratio (generally 0.20‰ [2SD]) were achieved, which are comparable to off-line Ca separation and continuous measurement. The reproducibility of the proposed analytical procedure was verified by measuring the SRM 915a standard as a sample randomly over 3months (n=56). Applicability of the protocol was demonstrated for matrix-rich natural water samples, coral samples, and bone standard reference materials.

On-line Preconcentration of Pd(II) Using Polyamine Silica Gel Filled Mini Column for Flame Atomic Absorption Spectrometric Determination

In this study, a novel method for determination of Pd(II) ions at trace level was developed using on-line preconcentration onto polyamine group bonded silica (PA-SG) with flame atomic absorption spectrometric determination. Pd(II) uptake dynamics of PA-SG was studied batchwise by investigating acidity and chloride concentration of aqueous phase, contact time and initial concentration of Pd(II) ions. The Pd(II) adsorption capacity of PA-SG was found to be 158.7 mg g−1 from aqueous phase containing 0.1 M HCl. The on-line preconcentration procedure of Pd(II) was optimized with main analytical parameters including sample and eluent flow rate, eluent type and volume and matrix ions. The optimum eluent type and flow rate of sample and eluent were found to be 1.0% thiourea in 1.0 mol L−1 HCl and 7.5 mL min−1, respectively. The preconcentration factor and sampling frequency were calculated to be 23.9 and 20 h−1, respectively. The calibration graph was linear over the range 10–200 µg L−1. The limits of detection (3σ) and quantification (10σ) values were computed to be 3 µg L−1 and 10 µg L−1, respectively. The RSD, % was found to be 4.6% for five measurement of 25 µg L−1 of Pd(II) ions. The accuracy of the developed method was successfully checked by determination of Pd(II) level of certified reference material platinum ore (SARM 7B). The proposed method was successfully applied for Pd(II) determination in various environmental water samples such as river, lake, sea and tap water, and spent auto catalyst.

Alignment of the CMS Tracker: Latest Results from LHC Run-II

The all-silicon design of the tracking system of the CMS experiment provides excellent measurements of charged-particle tracks and an efficient tagging of jets. Conditions of the CMS tracker changed repeatedly during the 2015/2016 shutdown and the 2016 data-taking period. Still the true position and orientation of each of the 15 148 silicon strip and 1440 silicon pixel modules need to be known with high precision for all intervals. The alignment constants also need to be promptly re-adjusted each time the state of the CMS magnet is changed between 0T and 3.8 T. Latest Run-II results of the CMS tracker alignment and resolution performance are presented, which are obtained using several millions of reconstructed tracks from collision and cosmic-ray data of 2016. The geometries and the resulting performance of physics observables are carefully validated. In addition to the offline alignment, an online procedure has been put in place which continuously monitors movements of the pixel high-level structures and triggers updates of the alignment constants if certain thresholds are exceeded.

An on-line coupling of nanofibrous extraction with column-switching high performance liquid chromatography – A case study on the determination of bisphenol A in environmental water samples

Polyamide 6 nanofiber polymers were used as modern sorbents for on-line solid phase extraction (SPE) coupled with liquid chromatography. The on-line SPE system was tested for the determination of bisphenol A in river water samples. Polyamide nanofibers were prepared using needleless electrospinning, inserted into a mini-column cartridge (5 × 4.6mm) and coupled with HPLC. The effect of column packing and the amount of polyamide 6 on extraction efficiency was tested and the packing process was optimized. The proposed method was performed using a 50-µL sample injection followed by an on-line nanofibrous extraction procedure. The influence of the washing mobile phase on the retention of bisphenol A during the extraction procedure was evaluated. Ascentis® Express C18 (10cm × 4.6mm) core-shell column was used as an analytical column. Fluorescence detection wavelengths (λex = 225nm and λem = 320nm) were used for identification and quantification of Bisphenol A in river waters. The linearity was tested in the range from 2 to 500µgL−1 (using nine calibration points). The limits of detection and quantification were 0.6 and 2µgL−1, respectively. The developed method was successfully used for the determination of bisphenol A in various samples of river waters in the Czech Republic (The Ohře, Labe, Nisa, Úpa, and Opava Rivers).

EOG signal Modeling using Double Exponential Smoothing for Robot Arm Control System

This paper present a novel way of modeling EOG signal to use in a robot arm control system, two procedures implemented, offline procedure to measure and modeling EOG for building a pattern reference model ,and online procedure used to Control the robot arm. By comparing online measured EOG and the EOG pattern in reference model suitable manipulation instruction generated by the micro controller. The double exponential smoothing method used for building the pattern reference model, the accuracy of the reference model tested with main squire error (MSE) and main absolute error (MAPE) measures. Auto correlation analysis applied to study the existing pattern and linearity of EOG signal with eye movements. EOG signal measurement for this research classified in to five kinds: EOG horizontal (left and right) Vertical (up and down), and blinking. The EOG signal models of this research saved and used as a reference model file to classify the eye movements. a measurement and robot arm control system constructed by using arduino olimix 328, olimix sensor shield, and robot arm driving circuit, arduino C used as a programming environment, Minitab software used to build the model and correlation analysis ,Brain Bay software used to control and signal processing.

Face Image Retrieval of Efficient Sparse Code words and Multiple Attribute in Binning Image

ABSTRACT In photography, face recognition and face retrieval play an important role in many applications such as security, criminology and image forensics. Advancements in face recognition make easier for identity matching of an individual with attributes. Latest development in computer vision technologies enables us to extract facial attributes from the input image and provide similar image results. In this paper, we propose a novel LOP and sparse codewords method to provide similar matching results with respect to input query image. To improve accuracy in image results with input image and dynamic facial attributes, Local octal pattern algorithm [LOP] and Sparse codeword applied in offline and online. The offline and online procedures in face image binning techniques apply with sparse code. Experimental results with Pubfig dataset shows that the proposed LOP along with sparse codewords able to provide matching results with increased accuracy of 90%.

On-line prognosis of fatigue crack propagation based on Gaussian weight-mixture proposal particle filter

Accurate on-line prognosis of fatigue crack propagation is of great meaning for prognostics and health management (PHM) technologies to ensure structural integrity, which is a challenging task because of uncertainties which arise from sources such as intrinsic material properties, loading, and environmental factors. The particle filter algorithm has been proved to be a powerful tool to deal with prognostic problems those are affected by uncertainties. However, most studies adopted the basic particle filter algorithm, which uses the transition probability density function as the importance density and may suffer from serious particle degeneracy problem. This paper proposes an on-line fatigue crack propagation prognosis method based on a novel Gaussian weight-mixture proposal particle filter and the active guided wave based on-line crack monitoring. Based on the on-line crack measurement, the mixture of the measurement probability density function and the transition probability density function is proposed to be the importance density. In addition, an on-line dynamic update procedure is proposed to adjust the parameter of the state equation. The proposed method is verified on the fatigue test of attachment lugs which are a kind of important joint components in aircraft structures.

An on-line orthogonal wavelet denoising algorithm for high-resolution surface scans

This paper deals with noise detection and threshold free on-line denoising procedure for discrete scanning probe microscopy (SPM) surface images using wavelets. In this sense, the proposed denoising procedure works without thresholds for the localisation of noise, as well for the stop criterium of the algorithm. In particular, a proposition which states a constructive structural property of the wavelets tree with respect to a defined seminorm has been proven for a special technical case. Using orthogonal wavelets, it is possible to obtain an efficient localisation of noise and as a consequence a denoising of the measured signal. An on-line denoising algorithm, which is based upon the discrete wavelet transform (DWT), is proposed to detect unavoidable measured noise in the acquired data. With the help of a seminorm the noise of a signal is defined as an incoherent part of a measured signal and it is possible to rearrange the wavelet basis which can illuminate the differences between its coherent and incoherent part. In effect, the procedure looks for the subspaces consisting of wavelet packets characterised either by small or opposing components in the wavelet domain. Taking real measurements the effectiveness of the proposed denoising algorithm is validated and compared with Gaussian FIR- and Median filter. The proposed method was built using the free wavelet toolboxes from the WaveLab 850 library of the Stanford University (USA).