Addition Of Olive Oil Research Articles

Carotenoid profile of tomato sauces: effect of cooking time and content of extra virgin olive oil.

The consumption of carotenoid-rich vegetables such as tomatoes and tomato sauces is associated with reduced risk of several chronic diseases. The predominant carotenoids in tomato products are in the (all-E) configuration, but (Z) isomers can be formed during thermal processing. The effect of cooking time (15, 30, 45 and 60 min) and the addition of extra virgin olive oil (5% and 10%) on the carotenoid extractability of tomato sauces was monitored using liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) and LC-ultraviolet detection (LC-UV). The thermal treatment and the addition of extra virgin olive oil increased the levels of antioxidant activity, total carotenoids, Z-lycopene isomers, α-carotene and β-carotene. These results are of particular nutritional benefit since higher lycopene intake has been associated with a reduced risk of lethal prostate and a reduction of prostate-specific antigen (PSA) levels. Moreover, β-carotene has been reported to suppress the up-regulation of heme oxygenase-1 gene expression in a dose dependent manner and to suppress UVA-induced HO-1 gene expression in cultured FEK4.

USING OF HONEY AND OLIVE OIL IN PRODUCTION OF SYNBIOTIC YOGHURT

The effect of fortification with both honey and olive oil on yoghurt quality was investigated. Control yoghurt was made using classic yoghurt culture and whole milk. Fou other yoghurt treatments were made by ABT culture and whole milk fortified with 0+0, 2+1, 4+2, and 6+4% of honey and virgin olive oil respectively. The sixth treatment was prepared using ABT culture and skim milk contained 6% honey + 4% virgin olive oil. Changes in rheological, chemical, microbial and organoleptic properties of yoghurt were monitored during refrigerated storage (4°C) for 15 days. Mixing of yoghurt milk with honey and olive oil to milk showed a slight decrease in the rate of acidity development. Fortification of milk with honey and olive oil had no clear effect on coagulation time, but increased curd tension and decreased curd syneresis values. Addition of honey and olive oil increased acidity, TS, fat, ash and TVFA contents of the examined yoghurt while there were no differences in TN and WSN values. Yoghurt made with honey and olive oil had lower TVBC, moulds and yeasts numbers as compared with control. Adding of honey and olive oil stimulated the growth and viability of bifidobacteria which helped in maintenance their counts above the recommended levels (106cfu.g-1) for a probiotic effect. Finally, supplementation of yoghurt with honey and olive oil greatly improved the sensory evaluation scores.

Microbiological and physicochemical characterization of dry-cured Halal goat meat. Effect of salting time and addition of olive oil and paprika covering

The objective of this work was to define a simple technological process for dry-cured Halal goat meat elaboration. The aims of this study were to analyze physicochemical parameters and to enumerate the microbial population at the end of the different manufacturing processes (two salting times and the addition of olive oil and paprika covering) on 36 units of meat product. A total of 532 strains were isolated from several selective culture media and then identified using classical and molecular methods. In general, salt effect and the addition of olive oil and paprika were significant for all the studied microbial groups as well as on NaCl content and water activity. Molecular analysis proves that staphylococci, especially Staphylococcus xylosus and Staphylococcus equorum, were the most common naturally occurring microbiota. The best manufacturing process would be obtained with a longer salting time and the addition of the olive oil and paprika covering.

Determining the Optimal Recipe for Long-Grain Jasmine Rice with Sea Tangle Laminaria japonica, and Its Effect on the Glycemic Index

Thai Jasmine rice (Oryza sativa, long grain Indica var.) is popular in southeastern Asia and China due to its non-glutinous, fluffy texture and fragrant smell. However it has a high starch digestibility, which leads to an increased glycemic index (GI). Therefore it may require modified cooking methods for diabetes patients. The objectives of this study were to optimize the ratio of Thai Jas mine rice, sea tangle, and olive oil (CLTR) based on consumers’ acceptance. The GI of plain cooked Thai Jasmine rice (CLR) was measured as a control. Sensory evaluation and response surface methodology were used to determine the optimal ratio. Texture analysis and nutritional evaluation were also performed on the optimal recipe of cooked Jasmine rice with sea tangle. A multiple regression equation was developed in quadratic canonical polynomial models. We used 26 trained Chinese panelists in their forties to rate color, flavor, adhesiveness, and glossiness, which we determined were highly correlated with overall acceptability. The optimal CLTR formula was 34.8% rice, 2.8% sea tangle, 61.9% water, and 0.5% olive oil. Compared to CLR, CLTR had a lower hardness, but a higher springiness and cohesiveness. However, CLR and CLTR had the same adhesiveness and chewiness. The addition of sea tangle and olive oil delayed retro-gradation of starch in CLTR and increased total dietary fiber, and protein and ash contents. The degree of gelatinization, and in vitro protein and starch digestibility of CLTR were lower than those of CLR. Based on Wolver’ method, the GI of CLTR (52.9, incremental area under the glycemic-response curve, ignoring the area below fasting, as used for calculating the GI [Inc]) was lower compared with that of CLR (70.94, Inc), which indicates that CLTR is effective in decreasing and stabilizing blood glucose level, owing to its lower degree of gelatinization and starch digestibility. Our results show that CLTR can contribute to the development of a healthier meal for families and the fast food industry.

Home Cooking and Phenolics: Effect of Thermal Treatment and Addition of Extra Virgin Olive Oil on the Phenolic Profile of Tomato Sauces

Tomato products are a key component of the Mediterranean diet, which is strongly related to a reduced risk of cardiovascular events. The effect of cooking time (15, 30, 45, and 60 min) and the addition of extra virgin olive oil (5 and 10%) on the phenolic content of tomato sauces was monitored using liquid chromatography coupled to tandem mass spectrometry. Concentration of phenolics in the tomato sauces decreased during the cooking process, with the exception of caffeic acid and tyrosol. The main degradation observed was the oxidation of quercetin, since the hydroxy-function at the C-ring of this flavonoid is not blocked by a sugar moiety, unlike rutin. Higher levels of virgin olive oil in tomato sauce seemed to enhance the extraction of phenolic compounds from the tomato, leading to higher phenolic contents in the sauces. Thus, the food matrix containing the phenolic compounds plays a crucial role in determining their accessibility.

“Dal Sulcis a Sushi”: Tradition and Transformation in a Southern Italian Tuna Fishing Community

“Dal Sulcis a Sushi”: Tradition and Transformation in a Southern Italian Tuna Fishing Community

Combined effects of high pressure processing and addition of soy sauce and olive oil on safety and quality characteristics of chicken breast meat.

This study was conducted to evaluate the combined effect of high pressure (HP) with the addition of soy sauce and/or olive oil on the quality and safety of chicken breast meats. Samples were cut into 100 g pieces and 10% (w/w) of soy sauce (SS), 10% (w/w) of olive oil (OO), and a mixture of both 5% of soy sauce and 5% olive oil (w/w) (SO) were pressurized into meat with high pressure at 300 or 600 MPa. Cooking loss was lower in OO samples than SS samples. With increased pressure to 600 MPa, the oleic acid content of OO samples increased. The total unsaturated fatty acids were the highest in SO and OO 600 MPa samples. Lipid oxidation was retarded by addition of olive oil combined with HP. The addition of olive oil and soy sauce followed by HP decreased the amount of volatile basic nitrogen during storage and reduced the population of pathogens. Sensory evaluation indicated that the addition of olive oil enhanced the overall acceptance and willingness to buy. In conclusion, the combination of HP with the addition of soy sauce and/or olive oil is an effective technology that can improve chemical, health, sensory qualities and safety of chicken breast.

The Quality of Transparent Soap from Farmer's Crude Calophyllum Seed Oil

<em>Calophyllum inophyllum</em> plants is one of the potential non-edible vegetable oil in Indonesia. Currently, the utilization of <em>C. inophyllum</em> seed oils directed for biofuels such as biodiesel feedstock. The oleochemical product diversification of <em>C. inophyllum</em> seed oil into transparent soap products is prospective to be developed, considering its utilization that does not compete with edible oil. The purpose of this research is to obtain the best conditions for the production process of <em>C. inophyllum</em> seed oil transparent soap. The research process is conducted in two phases. The first is the determination of the basic formula of transparent soap from <em>C. inophyllum</em> seed oil, and the second is the improvement of the organoleptic quality of the <em>Calophyllum</em> transparent soap. The research design used is the completely randomized factorial design with two treatments, i.e. the addition of olive oil and ethanol. The determination of the best formula for <em>Calophyllum</em> transparent soap is based on the weighting method on a number of physicochemical and organoleptic criteria. The results of the research show that the addition of olive oil and ethanol has a significant effect on the amount of <em>Calophyllum</em> transparent soap suds (P<0,05). The quality improvement of <em>Calophyllum</em> transparent soap is conducted with the addition of honey, fragrance, and coloring agent. The addition of honey has a significant effect on the transparency and color of <em>Calophyllum</em> transparent soap (P<0,05). The addition of fragrance types has a significant effect on transparency, color and aroma (P<0,05), but does not have a significant effect on the hedonic test on texture and impression after use (P>0,06). Based on the composite weighting method, the most preferred formula for <em>Calophyllum</em> transparent soap according to the panelists is the use of olive oil 5%, ethanol 15%, honey 0,3%, and floral fragrance 1%. This best condition for <em>Calophyllum</em> transparent soap are able to meet the standards of the Saudi Arabian Standard Organization (SASO) for the water content and evaporating substances, as well as free alkali.

The Effects of Dietary Oil Sources on Performance, Serum Corticosterone Level, Antibody Titers and IFN-γ Gene Expression in Broiler Chickens

The present study was performed to evaluate the effects of addition of fish oil, soybean oil and olive oil to diet on performance and immune response in broiler chickens. In a completely randomized design, 320 broiler chickens (Ross 308, 7 days old) were allocated to four dietary treatments (control and three vegetable oils), four replicates with 20 chicks per each. The results showed that body weight gain of chicks fed soybean oil was higher and feed conversion ratio was better than the other treatments in the total period (P 0.05). Chicks fed fish oil had higher relative weights of bursa of Fabricius and there were no significant differences in relative weights of spleen among treatments. It was concluded that the source of oils in the diet may be affect the performance and immune response, the addition of fish oil and soybean oil to the diet resulted in significant improvement of performance and immune response in broiler chickens, respectively.

Fat Reduction and Replacement by Olive Oil in Bologna Type Cooked Sausage. Quality and Nutritional Aspects

Eight different types of Bologna type cooked sausages apart from the control were produced in order to reduce the fat content (15%, 30%, 45%), replace the animal fat by olive oil (3%, 6.5% and 10%) as well as concomitant reduction and replacement of animal fat (30% of fat reduction with 2% of olive oil as an animal fat replacer). Quality attributes such as texture, water binding and color were monitored. Texture and water binding of fat reduced and fat replaced samples were quite similar to the control while color was strongly affected by the fat reduction and replacement. Fat reduction led to a redder cooked sausage and the addition of olive oil to a more yellow product. Chemical analysis revealed no major changes among samples apart from protein and fat content and fatty acid profile. Noteworthy, cooked sausage with more than 6.5% of olive oil achieved the World Health Organization’s recommendation on the nutritional fat index ((polyunsaturated + monounsaturated)/saturated fatty acid ≥ 2) which is very relevant to the development of healthier formulations. Cooked sausages with 45% of fat reduction and 30% fat reduced with 2% of olive oil were considered as the best by the panelists, which in addition had a balanced nutritional content by a lower caloric content. We concluded that fat reduction (up to 45%) and replacement (up to 10%) are possible with acceptable sensory quality and improved nutritional composition. When replacing animal fats by plant oils, the color of the product, which has a strong influence on the sensory acceptability of Bologna type cooked sausages, is affected. Therefore it must be controlled for a proper product development of meat products containing vegetable oils.

Electrospray ionization mass spectrometry and partial least squares discriminant analysis applied to the quality control of olive oil

Direct infusion electrospray ionization mass spectrometry in the positive ion mode [ESI(+)-MS] is used to obtain fingerprints of aqueous-methanolic extracts of two types of olive oils, extra virgin (EV) and ordinary (OR), as well as of samples of EV olive oil adulterated by the addition of OR olive oil and other edible oils: corn (CO), sunflower (SF), soybean (SO) and canola (CA). The MS data is treated by the partial least squares discriminant analysis (PLS-DA) protocol aiming at discriminating the above-mentioned classes formed by the genuine olive oils, EV (1) and OR (2), as well as the EV adulterated samples, i.e. EV/SO (3), EV/CO (4), EV/SF (5), EV/CA (6) and EV/OR (7). The PLS-DA model employed is built with 190 and 70 samples for the training and test sets, respectively. For all classes (1-7), EV and OR olive oils as well as the adulterated samples (in a proportion varying from 0.5 to 20.0% w/w) are properly classified. The developed methodology required no ions identification and demonstrated to be fast, as each measurement lasted about 3 min including the extraction step and MS analysis, and reliable, because high sensitivities (rate of true positives) and specificities (rate of true negatives) were achieved. Finally, it can be envisaged that this approach has potential to be applied in quality control of EV olive oils.

The importance of fatty acids in glycaemic response

AbstractMost diets worldwide are composed of high carbohydrate foods. Their consumption is associated with a rapid rise in blood glucose. The potential role that the degree of saturation of fatty acids may have in glycaemic response forms the basis of this paper. The addition of butter, olive oil or grapeseed oil significantly reduced the glycaemic index of white bread (white bread GI = 71; bread + butter GI = 53, bread + grapeseed oil GI = 58; bread+ olive oil GI = 50. The addition of olive oil had the greatest impact on GI. Olive oil has been widely recommended for its impact on blood lipids and cholesterol. Olive oil's influence on glycaemic response spawns a new line of research that should examine the role of lipids in carbohydrate metabolism.

Olive Oil, Flaxseed Oil 및 Perilla Oil을 급여한 Mice의 지질대사에 미치는 목초액의 영향

Abstract Our objectives in this experiment were to study the effects of oils that are high in contents of unsaturated fatty acids (olive, flaxseed, and perilla oils) and pyroligneous liquor on plasma lipid concentrations in mice. Male ICR-mouse (n=10 per group) were fed powdered form diets containing oil and pyroligneous liquor for 60 days. The cholesterol level of the PP group (96.71±25.75 ㎎/㎗) was lower than that of the COW group (133.56±21.53 ㎎/㎗). Levels of triglyceride of COP, CFP, and CPP groups were 121.10±50.79 ㎎/㎗, 77.80±38.58 ㎎/㎗, and 92.40±33.04 ㎎/㎗, respectively (p<0.05). The dietary addition of olive oil increased the plasma lsevels of cholesterol and LDL-cholesterol. On the other hand, the dietary addition of flaxseed oil and pyroligneous liquor increased the plasma level of HDL-cholesterol and decreased the plasma levels of LDL-cholesterol and triglyceride compared with those of the control group. Further, the dietary addition of perilla oil decreased plasma triglyceride but increased HDL-cholesterol. The decrease in the cholesterol level was much higher in the CFP group. In conclusion, the dietary addition of long-term pyroligneous liquor effectively decreased the plasma levels of cholesterol and triglyceride and increased the plasma level of HDL-cholesterol. The additional dietary administration of flaxseed and perilla oils increased the effect of pyroligneous liquor.Key words: pyroligneous liquor, flaxseed oil, perilla oil, cholesterol, triglyceride

Detection of Key Factors Affecting Lycopene in Vitro Accessibility

On the basis of a Plackett-Burman experimental design for a resolution IV level obtained via a foldover strategy, the effect of 11 factors on lycopene in vitro accessibility was investigated. The selected factors were thermal treatment (X1), olive oil addition (X2), gastric pH (X3), gastric digestion time (X4), pepsin concentration (X5), intestinal pH (X6), pancreatin concentration (X7), bile salts concentration (X8), colipase addition (X9), intestinal digestion time (X10), and intestinal digestion speed (X11). Tomato passata was used as a natural source of lycopene. Samples were collected after gastric and intestinal digestion, and from the micellar phase, to quantify the (all-E)-lycopene and its (Z)-isomers by HPLC. Except for X3, X6, X7, and X11, the other factors studied explained lycopene in vitro accessibility, mainly regarding intestinal digestion, with R(2) values ≥ 0.60. Our results showed that the accessibility of lycopene is influenced by the conditions applied during in vitro intestinal digestion.

Does olive oil prevent earwax build-up? An experimental study

Aim: An experimental study was conducted to determine whether daily lubrication of the external ear canal with olive oil enhances the normal expulsion of earwax. Method: Fifty people over the age of 50 years with bilateral occlusive earwax were recruited from 483 people attending ear care exhibitions in Essex and South Yorkshire. Earwax was initially removed from all ears following an immediately prior spray of olive oil. Each participant sprayed a controlled dose of olive oil 0.05 ml into the same ear each night for 24 weeks. The other ear was cared for in the individual's normal way and served as the control. Ears were examined at 8, 16 and 24 weeks using video otoscopy. Pre- and post-experiment interviews were conducted. Results: Nightly olive oil lubrication increased occlusion of the ear canal demonstrated through video otoscope image capture. The olive oil did not enhance the normal expulsion of earwax; on the contrary, the addition of olive oil increased the ear canal contents. This was confirmed by the median weight of ear canal contents removed at 24 weeks from the treated ears being 99.5% greater than the median weight of ear canal contents removed from the control ears. However, rather than using the olive oil for an extended period before earwax removal, it was found that all the earwax could be easily removed at first presentation by a spray of oil into the ear canal immediately before the earwax is removed. Conclusions: Regular olive oil lubrication increased the ear canal contents. However, olive oil sprayed into the ears immediately before removing wax enabled total wax removal. There is a need for further research to provide improved evidence for ear care.

Effects of dietary olive oil on growth performance, carcass parameters, serum characteristics, and Fatty Acid composition of breast and drumstick meat in broilers.

This experiment was conducted to evaluate the effects of dietary olive oil on growth performance, carcass parameters, serum characteristics, and fatty acid composition of breast and drumstick meat in broiler chickens. A total of 480 broilers were randomly allotted into three dietary treatments, including T (basal diet, 5% tallow), O1 (2% olive oil+3% tallow), and O2 (5% olive oil). During d 0 to 21, broilers fed the diet supplemented with 5% olive oil showed lower (p<0.05) body weight gain (BWG) and feed intake (FI) compared with those fed the T diet. Serum triglyceride concentration was reduced (p<0.05), while high density lipoprotein (HDL)-cholesterol concentration was increased (p<0.05) in the O2 treatment group compared with the T and O1 treatment groups. The addition of olive oil to the diets induced a reduction (p<0.05) in the total saturated fatty acid (SFA) contents in breast and drumstick meat, and increased (p<0.05) the total unsaturated fatty acid (USFA) contents and USFA/SFA ratios. In conclusion, a diet with 5% olive oil could decrease BWG and FI of broilers during the starter period (wk 0 to 3), and cause an increase in the serum HDL-cholesterol level, while decreasing the serum triglyceride concentration. Furthermore, USFA level and USFA/SFA ratios in breast and drumstick meat were increased by dietary supplementation of 2 or 5% olive oil.

Semen Quality and Sperm Function Loss by Hypercholesterolemic Diet Was Recovered by Addition of Olive Oil to Diet in Rabbit

Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes were also associated with a reduction in motility percentage and appearance of abnormal sperm morphology. The present study was carried out to evaluate the effect of dietary olive oil (OO, 7% v/w) administration to several male hypercholesterolemic rabbits (hypercholesterolemic rabbits, HCR) with altered fertility parameters. These HCR males were achieved by feeding normal rabbits with a high-fat diet (0.05% chol). HCR were associated with a modest non-significant increase in body weight (standard diet, 4.08±0.17 Kg, versus high-fat diet, 4.37±0.24 Kg). Hypercholesterolemic rabbits presented a marked decrease in semen volume, sperm cell count, and percentage of sperm motility, associated with a significant increase in sperm cell abnormalities. Moreover, sperm capacitation measured by the characteristic phosphorylated protein pattern in and induced acrosomal reaction were also altered suggesting sperm dysfunction. However, the administration of OO (for 16 weeks) to rabbits that were fed with 50% of the high-fat diet normalized serum chol. Curiously, OO supply succeeded to attenuate the seminal and sperm alterations observed in HCR group. Administration of OO alone did not cause any significant changes in above mentioned parameters. These data suggest that OO administration to HCR male rabbits recovers the loss of semen quality and sperm functionality.

Prosthetic Application of Newly Experimental Modeling Wax with some Additives( Medical Grass Extract)

Aims of the study: preparation of a new experimental modeling wax from hard paraffin, beeswax, starch, and medical Grass Extract (olive oil) and study the linear thermal expansion and melting point properties of the prepared wax. Materials and Methods : The samples were prepared ( 30 samples) by mixing Iraqi natural waxes (hard paraffin and beeswax) with additives (starch and olive oil) using spe-cial percentages proportions determined according to this study and using mold prepared according to ADA specification No.24, and the melting range and thermal expansion of these samples measured then compared with commercial modeling wax (SHANGCHI). Results: The new experimental model-ing wax 1 ( 80% hard paraffin + 15% beeswax + 5% olive oil) have minimum and maximum melting point mean value (59.8 – 63.6 °c) near to control (commercial modeling wax) (58 -63.4 °c) and the new experimental modeling wax 2 (90% beeswax + 5% starch + 5% olive oil) have linear thermal expan-sion mean value (0.186%) near to control (SHANGCHI) (commercial modeling wax) (0.204%). Con-clusions : The addition of olive oil to new experimental modeling wax 1(80% hard paraffin + 15% beeswax + 5% olive oil) lead to increase the linear thermal expansion and reduce the melting point, and the addition of olive oil to new experimental modeling wax 2 (90% beeswax + 5% starch + 5% olive oil) lead to decrease thermal expansion and reduce melting point.

식물성 유지 첨가 후 초고압 처리가 우육의 안전성 및 육질에 미치는 영향

본 연구는 식물성 유지를 첨가한 저급우육 등심의 초고압 처리가 저급우육의 물리화학적, 미생물학적 품질에 미치는 영향을 평가하기 위해서 실시되었다. 저등급 우육 중량의 10%(w/w) 식물성유지인 올리브유와 포도씨유를 첨가 하여 진공포장 후 600 MPa의 초고압에서 처리하였으며 0.1 MPa 처리군과 표면 육색, 조직감, 지방산, 관능평가, 일반 미생물 및 병원성 미생물학적 품질차이검사를 실시하였다. 표면 육색은 600 MPa 처리군이 0.1 MPa 처리군에 비해 <TEX>$L^*$</TEX> 값과 <TEX>$b^*$</TEX> 값이 유의적으로 높았으며 <TEX>$a^*$</TEX> 값은 유의적으로 낮았으며(p<0.05), 저장 10일 후에도 유사한 경향을 보였다. 초고압 처리된 우육의 조직감은 초고압 무처리구(0.1 MPa)와 유의적인 차이가 없었다(p>0.05). 지방산 조성 분석 결과 600 MPa 처리군중 올리브유 첨가구와 포도씨유 첨가구에서 각각 oleic acid와 linoleic acid가 유의적으로 높게 분석되었다(p<0.05). 일반 미생물학적 품질 평가 결과 저장 초기 600 MPa 처리군에서 검출되지 않았으나 0.1 MPa 처리군에서 3 Log CFU/g 수준으로 검출되었으며 저장 10일 후 0.1 MPa에서 6 Log CFU/g 수준까지 증가 한 반면 600 MPa 처리군 중 포도씨유 첨가구만 2 Log CFU/g 정도의 증가만 보였다. 병원성 미생물 접종시험 결과 600 MPa 처리시 E. coli와 L. monocytogenes 모두 저장 초기(저장 0일 차) 및 저장 10일 후 검출되지 않은 반면 0.1 MPa 처리군은 저장 초기에 초기 접종 농도인 7 Log CFU/g 수준으로 측정되었으며 저장 10일 후 역시 7 Log CFU/g 수준을 유지 하였다. 관능평가에서 종합적 기호도와 구매의사 항목에선 모든 시험구에서 유의적인 차이를 나타내지 못하였다(p>0.05). 연구결과 저급우육에 식물성 유지를 첨가하여 초고압 기술을 적용할 경우 병원성 미생물에 대한 안전성 확보와 식물성 유지 첨가에 의한 지방산 조성의 변화를 가져올 수 있다고 판단된다. 또한 올리브유 첨가를 통해 초고압 시 발생되는 지방산패를 억제할 수 있는 가능성을 보였다. Olive oil and grape seed oil (10% of meat weight) were added to a package of beef loin. The package was then vacuum-sealed, and high pressure was applied (HP, 600 MPa) to investigate the effect of the penetration of vegetable oil into meat and safety and quality of the meat. Non-HP (0.1 MPa) without any oil treatment was considered as a control. The color <TEX>$L^*$</TEX> and <TEX>$b^*$</TEX>-values of beef loin were higher and the <TEX>$a^*$</TEX>-value was lower than those of the control after HP at 600 MPa. The total aerobic bacterial number was 3 Log CFU/g in the control but no viable cell was detected in the beef with 600 MPa. All inoculated E. coli and L. monocytogenes were inactivated by HP. The beef loin with vegetable oil added without HP did not show any difference in fatty acid composition, but that treated by HP showed a higher oleic and linoleic acid content when olive oil and grape seed oil were added, respectively. The addition of olive oil inhibited lipid oxidation, and sensory evaluation revealed that there was no difference among treatments. The results indicate that the addition of vegetable oil followed by the application of HP enhances the safety of beef loin, changing the fatty acid composition in a health beneficial way. In addition, the use of olive oil can inhibit lipid oxidation induced by HP.

Changes in β-carotene bioaccessibility and concentration during processing of carrot puree

The effect of some process and product factors (addition of olive oil, high pressure homogenisation, subsequent thermal or high pressure pasteurisation) on β-carotene bioaccessibility and isomerisation of carrot puree was investigated. High pressure homogenisation could improve β-carotene bioaccessibility by disrupting cells, but only at a pressure higher than 50MPa. Softening of the cell walls during a subsequent thermal pasteurisation resulted in a further increase in β-carotene bioaccessibility. Unfortunately, the high temperature also induced formation of some undesirable cis-isomers. As cell walls are probably strengthened by high pressure, high pressure pasteurisation could not positively affect the amount of bioaccessible β-carotene. Moreover, the high pressure process induced β-carotene oxidation. A positive effect of olive oil on β-carotene bioaccessibility could only be noticed in combination with a thermal pasteurisation process when β-carotene was solubilised in the oil droplets. Under high pressure however, oil can be crystallised which hinders the solubilisation of β-carotene.