The first processing center in the mammalian olfactory system is the olfactory bulb (OB), a structure in which 2000 neuropil spheroids called glomeruli encode the input from 1000 types of olfactory receptor neurons, each specific for a particular chemical feature. Many imaging modalities have been used to simultaneously assess the activity of many of these channels and have provided insight into the way in which volatile odorants are represented in the OB. However, there has been little evaluation of the functional differences between animals or the extent to which given animal's response is conserved over multiple trials. Here we have used high-resolution fMRI to quantitatively evaluate the similarity of patterns produced by the same subject to a repeated stimulus and to compare the responses of different subjects to the same stimulus. Male Sprague-Dawley rats under urethane anesthesia were stimulated with iso-amyl acetate or carvone (-) in this study. All data were acquired on a modified 7 T Bruker Biospec. Imaging experiments were performed using fast low-angle single-shot (FLASH) gradient-echo sequence. T1-weighted FLASH anatomical images have resolution of 110110250 m3. Each fMRI experiment contained a series of 24 T2*-weighted FLASH images (resolution = 220220250 m3). The mean image of the pre-stimulation baseline images was subtracted from the stimulation images on a pixel-by-pixel basis to generate student t-maps, which were overlaid onto the corresponding anatomical images to locate the activated region in the OB. Activity maps of the entire glomerular layer were constructed with an algorithm that integrates the glomerular data from multiple slices. OB activity maps from the same subject were highly conserved from trial to trial using the odorant carvone (-) at the same concentration and exposure duration. A quantitative comparison of the fraction of pixels activated by both trials for multiple odorants yielded values from 48 to 60%. In contrast, there was more substantial variability when a given odorant was tested in multiple subjects. Figure 1 shows the response of multiple animals to an equivalent exposure of iso-amyl acetate. While there is considerable variability in the fraction of the OB involved and the intensity of that involvement (1a), when the images are equivalently scaled and averaged it becomes clear that there are certain shared regions of activity (1b). This is consistent with genetic studies in which olfactory receptor neuron projections vary greatly between individuals but still localize to broadly defined regions. In conclusion, the consistency of intra-subject patterns and the variability of inter-subject patterns suggest that the primary causes for variations in the responses to identical stimuli are neuroanatomical. These findings help to validate the use of fMRI in the study of olfaction and recommend caution in comparing patterns from different animals.