Fatty Oleic Acid Research Articles

Effect of anhydrous milk fat fraction addition to butter on water loss reduction

Anhydrous milk fat (AMF) and AMF fractionated at 20, 25, and 30 °C were used in butter making to evaluate their effect on water loss (WL) and physical properties of butter. AMF and AMF fractions were incorporated into butter either in the cream before churning or in the working step. When AMF and AMF fractions were added, the cream method resulted in samples with decreased WL with high melting AMF fractions; the worked procedure butters had decreased WL with lower melting AMF fractions. The cream method WL had a significant positive correlation with water content and a significant negative correlation with hardness, crossover point, solid fat content (5–20 °C), enthalpy, and content of palmitic and oleic fatty acids (P < 0.05). The WL of worked method had a significant positive correlation with hardness, solid fat content at 5–20 °C, and myristic fatty acids (P < 0.05).

Fatty acids and risk of dilated cardiomyopathy: A two-sample Mendelian randomization study.

Previous observational studies have shown intimate associations between fatty acids (FAs) and dilated cardiomyopathy (DCM). However, due to the confounding factors and reverse causal association found in observational epidemiological studies, the etiological explanation is not credible. To exclude possible confounding factors and reverse causal associations found in observational epidemiological studies, we used the two-sample Mendelian randomization (MR) analysis to verify the causal relationship between FAs and DCM risk. All data of 54 FAs were downloaded from the genome-wide association studies (GWAS) catalog, and the summary statistics of DCM were extracted from the HF Molecular Epidemiology for Therapeutic Targets Consortium GWAS. Two-sample MR analysis was conducted to evaluate the causal effect of FAs on DCM risk through several analytical methods, including MR-Egger, inverse variance weighting (IVW), maximum likelihood, weighted median estimator (WME), and the MR pleiotropy residual sum and outlier test (MRPRESSO). Directionality tests using MR-Steiger to assess the possibility of reverse causation. Our analysis identified two FAs, oleic acid and fatty acid (18:1)-OH, that may have a significant causal effect on DCM. MR analyses indicated that oleic acid was suggestively associated with a heightened risk of DCM (OR = 1.291, 95%CI: 1.044-1.595, P = 0.018). As a probable metabolite of oleic acid, fatty acid (18:1)-OH has a suggestive association with a lower risk of DCM (OR = 0.402, 95%CI: 0.167-0.966, P = 0.041). The results of the directionality test suggested that there was no reverse causality between exposure and outcome (P < 0.001). In contrast, the other 52 available FAs were discovered to have no significant causal relationships with DCM (P > 0.05). Our findings propose that oleic acid and fatty acid (18:1)-OH may have causal relationships with DCM, indicating that the risk of DCM from oleic acid may be decreased by encouraging the conversion of oleic acid to fatty acid (18:1)-OH.

Triacylglycerols hydrolysis and hydroxy- and epoxy-fatty acids release during lactic fermentation of plant matrices: An extensive study showing inter- and intra-species capabilities of lactic acid bacteria

This study aims to show that lactic fermentation by selected starters can enrich plant matrices with hydroxy- and oxo-fatty acids. The behavior of 31 lactic acid bacteria strains was investigated during the fermentation of Persian walnut, which was selected as a model growth substrate due to its inherent lipids content. The content of the following free fatty acids increased in the majority of the fermented walnut samples: linoleic, α-linolenic, palmitic, and oleic acids. The increase of diacylglycerols and, especially, monoacylglycerols levels in fermented walnuts confirmed that strain-specific bacterial lipolytic activities hydrolyzed triacylglycerols during walnut fermentation. Twelve hydroxylated or epoxidized derivatives arising from oleic, linoleic, and linolenic fatty acids, in five groups of isomeric compounds, were also identified. In addition to the better-known lactobacilli, certain strains of Weissella cibaria, Leuconostoc mesenteroides, and Enterococcus faecalis emerged for their lipolytic activities and ability to release hydroxy- and epoxy-fatty acids during walnut fermentation.

Microbial lipid production from soybean hulls using Lipomyces starkeyi LPB53 in a circular economy

Soybean hulls are lignocellulosic residuesgeneratedinthe industrial processing of soybean, representing about 5 % of the mass of the whole bean. This by-product isan importantsource of polymers suchas cellulose(34 %) and hemicellulose (11 %),which could bevalorizedvia biotechnology to improvethe economic returnof the oilseed chain. In the present work,soybean hulls were evaluated as a carbon sourcefor biolipid productionbyLipomycesstarkeyi LPB 53. Initially the hulls were treated physicochemically and enzymatically to obtain fermentable sugars. Subsequently, biomass growth was evaluated using different nitrogen sources andthe lipid production was optimized, reaching a maximum cell biomass concentration of 26.5 g/L with 42.5 % of lipids. Around 65 % of the xylose content was consumed.The obtained oil wasmajorlycomposed of oleic, palmitic, palmitoleic, linoleic and stearic fatty acids in a proportion of 54 %, 32 %, 4 %, 3 % and 2 %, respectively.

Obtaining Biodiesel from Fat Extracted from Solid Waste Produced in the Fleshing Stage of Leather Manufacturing

Tallow is produced in leather manufacturing, particularly in the fleshing stage. It contains large amounts of fat, which, in most industrial plants, pollutes water and soil due to poor disposal. In order to take advantage of this solid waste, chemical processes have been applied to transform the fats extracted from tallow into biodiesel. In this work, an extraction technique involving non-polar solvents pressurized at 15 psi. With gasoline, a 51,0% fat recovery was obtained. The quality of the extracted fat was determined by analyzing density, viscosity, melting point, moisture, acidity, and acidity, peroxides, saponification, and iodine indices. Biodiesel was obtained via transesterification reactions with methanol and sodium hydroxide. The content of methyl esters produced in the reaction was characterized by means of gas chromatography coupled with mass spectrometry, where the methyl esters of palmitic and oleic fatty acids were identified as major components.

Optimization Studies and Compositional Oil Analysis of Pequi (Caryocar brasiliense Cambess) Almonds by Supercritical CO2 Extraction.

Caryocar brasiliense Cambess (pequi) is the fruit of the pequizeiro tree found in the Brazilian Cerrado (savanna). Supercritical fluids have been used to effectively extract bioactive chemicals. In light of the paucity of research on the supercritical extraction of pequi, in this study, experimental tests were conducted on the extraction of pequi almond oil using supercritical CO2; the optimal extraction conditions were determined, and the fatty acids and active compounds in the oil were characterized. The experiments were conducted using the Box-Behnken experimental design of a three-variable system: pressure (15, 20, and 25 MPa), temperature (303.15, 318.15, and 333.15 K), and flow rate (2, 3, and 5 g.min-1). The optimal extraction conditions were 318.15 K, 25 MPa, and 5.0 g.min-1, which yielded 27.6 wt% of oil. The experimental kinetic curves were described using a second-order quadratic model (based on the Sovová model), which demonstrated a satisfactory correspondence with the kinetic curves. Significant amounts of squalene, stigmasterol, oleic fatty acids, and palmitic fatty acids were detected in pequi almond oil.

Влияние фитовеществ на биохимический состав мышечной ткани цыплят-бройлеров

Abstract. The aim of the study is to evaluate the effect of phytobiotics on the biochemical composition of the muscle tissue of broiler chickens. Research methods: atomic emission spectrometry, capillary electrophoresis, gas chromatography. Scientific novelty. The influence of phytomaterials (gamma-octalactone, vanillic acid) on the biochemical composition of the muscle tissue of broiler chickens was evaluated for the first time. Results: biochemical composition of muscle tissue the composition was characterized by a lower content of amino acids: tyrosine (pectoral muscles), lysine, threonine, alanine, leucine + soleucine (femoral muscles) in group II (P &lt; 0.05) and a high content of valine and histidine (pectoral muscles) in group I (P &lt; 0.05), according to compared to the control. There was also an increase in the concentration of fatty acids in the pectoral muscle (P &lt; 0.05): myristic and arachin (I and II), stearic (III), linoleic (II and III) and a decrease in palmitic (III) and palmitooleic (II and III). An increase in the concentration of myristic (III group, P &lt; 0.05), oleic (II group, P &lt; 0.05) fatty acids was found in the thigh muscles. In group I broiler chickens, the concentration of macronutrients (Ca, P, K, Na) and trace elements (B, Fe) in the pectoral muscle decreased (P ≤ 0.05). In group II, an increase was found (P ≤ 0.05) – Ca, Co, Mn, Zn, in group III – Fe (P ≤ 0.05). In the thigh muscle tissue of group I, the concentrations of macronutrients – K, Mg decreased (P ≤ 0.05) and increased (P ≤ 0.05) Na, Ca, B, Cu, Ni, I, Zn. In II, there was an increase in the content of B (P &lt; 0.05), and in III, on the contrary, a decrease (P ≤ 0.05) in Ca and K. Thus, the use of vanillin acid alone or in combination with gamma-lactone contributes to the biological value of the muscle tissue of broiler chickens in terms of increasing the amount of essential amino acids, unsaturated fatty acids and important macro- and microelements.

Quality indicators of traditional Bulgarian artisanal sheep’s cheese

The aim of the present study is focused on the evaluation of the quality parameters of artisan sheep cheese produced by old Bulgarian technology. The study was carried out in three stages - at the end of the ripening process (45th day), during and at the end of storage (180th and 360th day). An increase of 9.0% in dry matter and a decrease in water content and titratable acidity values were observed over the entire period studied. There was a decrease in oleic fatty acid (C18:1n9c) from 28.16% at day 45 to 26.09% at day 360 of ripening. In the case of palmitic acid (C16:0) the values recorded were similar from 30.24% (45th day) to 30.74% (360th day). Changes in the composition of organic acids were observed, with lactic acid levels decreasing from 27.66% at day 45 to 8.62% at day 360. After microbiological analysis, it was found that the main microflora present in the samples were representatives of lactic acid microorganisms as compared to the non-starter microflora.

Papaya seeds (Carica papaya L. var. Formosa) in different ripening stages: unexplored agro-industrial residues as potential sources of proteins, fibers and oil as well as high antioxidant capacity

The use of whole fruits and vegetables, including the parts usually discarded during food processing, is an alternative to reduce the quantity of agro-industrial wastes. This study aimed to evaluate papaya seeds' nutritional and bioactive potential in two ripening stages. The seeds in the stages 0 and 5 of ripening were analyzed regarding their physicochemical composition, while the oil and the hydroethanolic extracts of the seeds were studied in respect of their fatty acid profile, total phenolic content, antioxidant and antimicrobial capacities. The seeds in both ripening stages show good nutritional quality, because they are sources of protein, fiber, and oil. The oil extracted from the seeds is majorly composed by oleic fatty acid (around 70%). The seeds extracts did not present antimicrobial activity against Salmonella enteritidies, Escherichia coli e Staphylococcus aureus. However, they presented high contents of total phenols (58.1 and 36.0 mg GAE⁄g dry extract for seeds in the ripening stages 0 and 5, respectively) and good antioxidant capacity, according to the FRAP and ABTS●+ assays. Papaya seeds provide nutrients and bioactive compounds and their use is a promising alternative to reduce the disposal of food wastes in the environment.

Preliminary Analysis of Unsaturated Fatty Acid Profiles of Coffea arabica L., in Samples with a Denomination of Origin and Speciality of Oaxaca, Mexico.

In February 2020, Coffea arabica L. grown on the coast and in the Southern Sierra Madre of the state of Oaxaca, Mexico obtained the denomination of origin. Which does not have data on color and chemical composition, the first associated with the degree of roasting and the second with lipids (17-18%), as the group of compounds responsible, in part, for flavor, consistency, and may contribute to health benefits. In the present work, color was determined on the CIE L*a*b* scale and the unsaturated fatty acids by Nuclear Magnetic Resonance (NMR) of 1H and 13C in samples of medium roasted specialty coffee from the "Pluma" coffee-growing region, Oaxaca, Mexico. The average value of L* luminosity in ground coffee was 42.1 ± 0.1 reported for a light roast. Unsaturated fatty acids were quantified from the lipid fraction of the gr1 ound grain by NMR 1H and 13C, obtaining on average the highest abundance of linoleic (41.7 ± 0.5 by 1 H and 41.24 ± 0.5 by 13C), followed by oleic (9.2 ± 0.2 by 1H and 7.4 ± 0.2 by 13C) and linolenic (1.5 ± 0.1 by H and 1.1 ± 0.2 by 13C). This study indicates that 1H and 13C NMR spectroscopy is a useful tool for the quantification of linolenic, linoleic, and oleic fatty acids by the method of key signal shifts of these acids found in lipid samples in roasted coffee grains.

Evaluation of the sensory properties, volatile aroma compounds and functional food potentials of cold-press produced mahaleb (Prunus mahaleb L.) seed oil

The aims of this study were to cold-press mahaleb seed, and then fully characterize the oil to extent its food and functional food applications. The novelty of this study relies upon the first data provided by the thermal analysis, sensory analysis, and volatile aroma compounds composition. The seeds were pressed with a screw-type single-head press with a maximum oil exit temperature of 40 °C. Most common physico-chemical properties, composition analyses, volatile aroma compounds profile, sensory descriptive analysis and consumer tests were completed. The main properties were appropriate and the composition (fatty acids, phytosterols and tocopherols) data concurred with the available literature. Thermal data were provided, and the oil peak crystallization and melting temperatures were −44.45 °C and −8.41 °C, respectively. There were 38 volatile aroma compounds quantified mostly with almond, green, vanillin, woody, and fermented aroma definitions. The panel described the oil with 5 sensory descriptive (almond, vanillin, dough, green, cooling) terms. Consumers liked appearance the most (4.49) with a general acceptance score of 3.70 on a 5-point hedonic scale. Overall, the mahaleb seed oil is a conjugated linolenic, oleic and linoleic fatty acids, β-sitosterol and γ-tocopherol rich, very aromatic, and consumer-liked sample. Further studies with various food applications are foreseen.

The composition of monounsaturated fatty acids of artemia enriched with biologically active substances

The work is devoted to the study of monounsaturated fatty acids (MUFA) content in artemia enriched with biologically active substances at different stages of ontogenesis. Artemia cysts are used in the preparation of dietary supplements, highly effective feed additives for farm animals, fertilizers for crop production, chitosan for pharmaceutical industry and cosmetology, as raw materials in many other fields. When growing fish, crustaceans, shellfish in aquaculture, artemia nauplii are used as live starter feeds, which are obtained in situ when cultivating artemia cysts. Recently, the direction of enrichment of artemia nauplia used for feeding fish larvae and fry with biologically active substances has begun to develop: vitamins, essential amino acids, essential fatty acids, probiotics. In our work, we used a feed additive for the enrichment of artemia nauplia, including: vitamin-amino acid complex – «Chiktonic», probiotic – «Vetom-1», adaptogen – «Trekrezan», hemp oil. Enrichment was carried out through the skin at the stage of early nauplia. The aim of the work was to study the composition of MUFA at different stages of ontogenesis in intact artemia and artemia enriched with biologically active substances. The main function of MUFA is to activate metabolic processes and maintain homeostasis. When identifying monounsaturated fatty acids of artemia, the race we studied, the following were identified: oleic, palmitoleic, eicosenic, elaidic, erucic, nervonic, myristoleic acids. Oleic, palmitoleic and eicosenic fatty acids played a dominant role in the structure of MUFA. Two fatty acids, erucic and elaidic, can be distinguished in the composition of MUFA, the content of which was minimal.

Assessment of the effect of pre-slaughter fasting period of young bulls on the qualitative characteristics of meat

The purpose of the study was to evaluate the effect of the technological operation “pre-slaughter fasting period” on the qualitative characteristics of the meat of young bulls. The studies were carried out in two groups of Ayrshire bulls of 18 months of age, with a live weight of 435 ± 3.25 kg: I (n = 10) – slaughter without fasting period at the slaughter station, II (n = 10) – slaughter with 24-hour fasting period at the slaughter station. The total distance from the farm to the animal weighing and slaughter station was 97 km, and the travel time was 1 hour 43 minutes. The ambient temperature at the time of transportation was minus 13-15 °C, air humidity was 79 %, wind speed was 6 m/s. It has been established that the technological operation in the form of fasting period during 24 hours after transportation affected the qualitative indicators of the longissimus dorsi muscle, namely, there increased: the dry matter content by 2.45 %, protein – by 2.64 %, glycogen concentration – by 38.9 %, moisture capacity – by 11.01 %, oleic fatty acid – by 2.65 % with a decrease in moisture content – by 2.48 %, pH – by 3.5 %, myristic fatty acid by 0.95 %, palmitoleic acid – by 0.42 %, stearic acid – by 1.35 % compared to the group of bulls without fasting period. Thus, pre-slaughter 24-hour fasting period of young bulls improves the qualitative characteristics of meat compared to animals slaughtered without it.

Structural Basis of the Change in the Interaction Between Mycophenolic Acid and Subdomain IIA of Human Serum Albumin During Renal Failure.

Mycophenolic acid (MP) is an active metabolite of mycophenolate mofetil, a widely used immunosuppressive drug. MP normally exhibits high plasma protein binding (97-99%), but its binding rate is decreased in patients with renal insufficiency. This decreased protein binding is thought to be associated with leukopenia, a side effect of MP. In this study, we characterized the change in protein binding of MP in renal failure patients. Our findings indicate that MP binds strongly to subdomain IIA of human serum albumin. X-ray crystallographic data indicated that the isobenzofuran group of MP forms a stacking interaction with Trp214, and the carboxyl group of MP is located at a position that allows the formation of hydrogen bonds with Tyr150, His242, or Arg257. Due to the specific binding of MP to subdomain IIA, MP is thought to be displaced by uremic toxin (3-carboxy-4-methyl-5-propyl-2-furan-propionic acid) and fatty acids (oleate or myristate) that can bind to subdomain IIA, resulting in the decreased plasma protein binding of MP in renal failure.

Transformation of Hydrophilic Drug into Oil-Miscible Ionic Liquids for Transdermal Drug Delivery.

The transdermal delivery of hydrophilic drugs remains challenging owing to their poor ability to permeate the skin; formulation with oil media is difficult without adding chemical permeation enhancers or co-solvents. Herein, we synthesized 12 oil-miscible ionic liquid (IL) drugs comprising lidocaine-, imipramine-, and levamisole (Lev)-hydrochloride with fatty acid permeation enhancers, i.e., laurate, oleate, linoleate, and stearate as counterions. A set of in vitro and in vivo studies was performed to investigate the potency and deliverability of the transdermal drug formulations. All of the synthesized compounds were freely miscible with pharmaceutically acceptable solvents/agents (i.e., ethanol, N-methyl pyrrolidone, Tween 20, and isopropyl myristate (IPM)). In vitro permeation studies revealed that the oleate-based Lev formulation had 2.6-fold higher skin permeation capability than the Lev salts and also superior ability compared with the laurate-, linoleate-, and stearate-containing samples. Upon in vivo transdermal administration to mice, the peak plasma concentration, elimination half-life, and area under the plasma concentration curve values of Lev-IL were 4.6-, 2.9-, and 5.4-fold higher, respectively, than those of the Lev salt. Furthermore, in vitro skin irritation and in vivo histological studies have demonstrated that Lev-IL has excellent biocompatibility compared with a conventional ionic liquid-based carrier. The results indicate that oil-miscible IL-based drugs provide a simple and scalable strategy for the design of effective transdermal drug delivery systems.

Rice Landrace Varieties and Fatty Acid Diversity: Profiling of Fatty Acid Variability of Parboiled and Un-parboiled Abakaliki Rice Landraces in Nigeria

Rice (Oryza sativa) is among Nigeria's top priority staple crops that play a role in food security and livelihood resilience within the household. Different varieties of rice landraces are grown in Nigeria; Abakaliki rice is grown in Ebonyi State and consumed in almost all parts of the country. This study evaluated the fatty acid composition of parboiled and un-parboiled Abakaliki rice landraces. The GC-MS analysis revealed that myristic, myristoleic, oleic, linoleic and vaccenic fatty acids were the most dominant in the rice landraces. In addition, all study samples contain a total amount of vaccenic (34.45%), oleic (33.36%) and myristic (21.68%) fatty acid, however myristoleic (3.3%) and linoleic (7.5%) fatty acid is in low amount. This study reveals that these fatty acids are 63.82% higher in the parboiled rice cultivars than in the un-parboiled rice.

Artificial neural network and random forest regression models for modelling fatty acid and tocopherol content in oil of winter rapeseed

With the aid of models used in artificial intelligence, a wide range of data can be processed quickly with high accuracy. The quality of rapeseed oil from 40 genotypes cultivated during four consecutive years was analysed. Two machine learning techniques (artificial neural network – ANN, and random forest regression – RFR) were applied for the modelling of fatty acids content (C16:0; C18:0; C18:1; C18:2; C18:3 and C22:1), α-tocopherol, γ-tocopherol and total tocopherols, according to the data of production year and winter rapeseed genotype. The developed models exerted high-quality anticipation features, showing high r2 during the training cycle. The best fit between the modelled and measured traits for ANN model was observed for erucic acid content. RFR modelling for all fatty acids was more effective than ANN model, with the highest precision for palmitic, stearic, and oleic fatty acids (r2>0.9). This study emphasized the possibility of using ANN and RFR models to model winter rapeseed quality traits.

Single-cell RNA Sequencing Reveals a Role for Reactive Oxygen Species and Peroxiredoxins in Fatty Acid-induced Rat ß-cell Proliferation

Nutrients play an important role in β-cell compensation and dysfunction in obesity and type 2 diabetes, respectively. Previously, we demonstrated that fatty acids potentiate glucose-induced β-cell proliferation in response to nutrient surfeit in rats. In recent studies we identified the mono-unsaturated fatty acid oleate as a major driver of β-cell proliferation in rat islets ex vivo.

Enrichment of nervonic acid in Acer truncatum Bunge oil by combination of two-stage molecular distillation, one-stage urea complexation and five-stage solvent crystallization

Nervonic acid is the world's first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value. The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult. A new combined process of molecular distillation, urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials. First, according to the difference in the mean free path of fatty acids, molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18–C20 fatty acids of stearic, oleic, linoleic, and linolenic acids. The content of C16–C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes, in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%. Then, according to the difference in carbon chain length and saturation of fatty acid, the contents of C22–C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification (SPOMFs) ratio as 0.6. In addition, all saturated fatty acids were basically separated. Finally, according to the difference in the solubility of fatty acids in solvents, the C18–C20 unsaturated fatty acids of oleic, linoleic, and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization. The content of C18–C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization. The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization. The combined process of molecular distillation, urea inclusion and low temperature crystallization innovatively adopted an efficient, simple and easy-to-industrial solvent crystallization method to separate erucic and nervonic acids, obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.

Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry

High oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-based method that is feasible for identification of putative allergenic protein as well as semi-quantitation of five major allergen protein in three different Chinese high-oleic peanut cultivars (JH 13, JH 16 and JH 18) have been reported. Results show that three Chinese high-oleic acid peanut cultivars selected all contained highly allergenic proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The allergenic protein profiles of Chinese high-oleic acid peanut cultivars were very similar to that of conventional peanuts, but the allergenic protein subunits varied greatly among high-oleic peanuts. Additionally, a comprehensive peptide-filtering pipeline had been developed for identification of potential peptide markers in peanut allergen proteins. Through the peptide-filtering pipeline, three novel peptide markers, IVQIEAKPNTLVLPK, SSNPDIYNPQAGSLR and AQSENYEYLAFK surrogate to Ara h 1, Ara h 3 with high abundance, good MS response and highly reliability were identified, which can be used as candidate peptide markers for the detection of peanut allergens in different food matrices.