Abstract
In recent years, natural product's research gained momentum, fueled by technological advancement and open availability of research data. To date, sea buckthorn (Hippophae rhamnoides L. [Elaeagnaceae]) plant parts, especially berries, are well characterized and repeatedly tested for antioxidant activity and regenerative properties, in various cell types and tissues. However, fatty acids (FA) have been less investigated in term of biological effects, although, they are important bioactive components of the sea buckthorn fruit and oil. The aim of our work was to determine whether sea buckthorn seed oil is a suitable source of FA with regenerative properties on normal skin cells. Using high-performance liquid chromatography (HPLC) and liquid chromatography – mass spectrometry (LC-MS), we purified and characterized four fractions enriched in saturated (palmitic) and non-saturated (linoleic, alfa-linolenic, oleic) FA, which were tested for cytotoxicity, cytokine and growth factor production, and regenerative effect on normal keratinocytes and skin fibroblasts. Evidence is presented that the palmitic acid enriched fraction was a suitable sea buckthorn seed oil derived product with cell proliferation properties on both skin cell types.
Highlights
Accelerated development of biotechnologies during the last decades allowed thorough biochemical investigation of medicinal plants, such as sea buckthorn
In the conditions selected for preparative chromatography, linolenic acid eluted at 10.1 min, oleic, palmitic, and stearic acids eluted at 18.7, 21.7, and 33 min, respectively, while linoleic and palmitoleic acids were not separated, eluting together at 13.1 min (Figure 1)
A gradient method aimed at separating LA and palmitoleic acid (POA) was tested, but it resulted in high retention times and too much solvent consumption
Summary
Accelerated development of biotechnologies during the last decades allowed thorough biochemical investigation of medicinal plants, such as sea buckthorn SBT-Derived PA Stimulates Skin Cells Proliferation with bioactive properties, known to be phenolics, flavonoids, and carotenoids, as well as lipid soluble vitamins and their precursors (Guo et al, 2017; Criste et al, 2020) (reviewed in Zielińska and Nowak, 2017). They are important components of the sea buckthorn, fatty acids (FA) have been less investigated in terms of biological effects (for a recent review on their impact on human health, see (Solà Marsiñach and Cuenca, 2019). The aim of our work was to determine whether sea buckthorn seed oil is a suitable source of FA with potential regenerative properties on normal skin cells and whether these exploitation products are a match for commercially available analytical standards
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