Oleaginous Seeds Research Articles

Analysis of the efficiency of different Oleaginous seeds and shells for use as natural coagulants

The research problem is to reduce the use of chemical coagulants by seeking more sustainable alternatives to water treatment. The use of Moringa oleifera (MO) is a good alternative coagulant agent, indicated in the water treatment system, in its clarification process, in addition to being biodegradable, but there is a need to look for other seeds that also have potential for be used as natural coagulants, in this context, it is justified to study other typical seeds from Portugal, where this study was carried out, to be used as an alternative natural coagulant. The hypothesis of this project is: among the typically European seeds selected for evaluation, seeds with high potential for use as a natural coagulant for use in water treatment will be found. This project aims to identify seeds, typical of Portugal, with similar potential to Moringa oleifera Lam to be used as a natural coagulant. The project was carried out in partnership with UFS, IFS and ISEP, where experiments were carried out with synthetic water with turbidity ranging from 40 to 350 NTU. The seeds used were four different types of European chestnuts, American and Portuguese nuts, plantain and Moringa oleifera, in addition to chestnut and walnut shells. From the results found, the Spanish chestnuts stand out with a turbidity removal efficiency of up to 69.8% in high turbidity water, in addition to the MO with a removal efficiency of 89.8% in high turbidity water.

Analysis of the efficiency of different oleaginous seeds and shells for use as natural coagulants

Analysis of the efficiency of different oleaginous seeds and shells for use as natural coagulants

Quantitative Analysis of Biodiesel Adulterants Using Raman Spectroscopy Combined with Synergy Interval Partial Least Squares (siPLS) Algorithms

Biodiesel has emerged as an alternative to traditional fuels with the aim of reducing the impact on the environment. It is produced by the esterification of oleaginous seeds, animal fats, etc., with short-chain alcohols in an alkaline solution, which is one of the most commonly used methods. This increases the oxygen content (from the fatty acids) and promotes the fuel to burn faster and more efficiently. The accurate quantification of biodiesel is of paramount importance to the fuel market due to the possibility of adulteration, which can result in economic losses, engine performance issues and environmental concerns related to corrosion. In response to achieving this goal, in this work, synergy interval partial least squares (siPLS) algorithms in combination with Raman spectroscopy are used for the quantification of the biodiesel content. Different pretreatment methods are discussed to eliminate a large amount of redundant information of the original spectrum. The siPLS technique for extracting feature variables is then used to optimize the input variables after pretreatment, in order to enhance the predictive performance of the calibration model. Finally, the D1-MSC-siPLS calibration model is constructed based on the preprocessed spectra, the selected input variables and the optimized model parameters. Compared with the feature variable selection methods of interval partial least squares (iPLS) and backward interval partial least squares (biPLS), results elucidate that the D1-MSC-siPLS calibration model is superior to the D1-MSC-biPLS and the D1-MSC-iPLS in the quantitative analysis of adulterated biodiesel. The D1-MSC-siPLS calibration model demonstrates better predictive performance compared to the full spectrum PLS model, with the optimal determination coefficient of prediction (R2P) being 0.9899; the mean relative error of prediction (MREP) decreased from 9.51% to 6.31% and the root--mean-squared error of prediction (RMSEP) decreased from 0.1912% (v/v) to 0.1367% (v/v), respectively. The above results indicate that Raman spectroscopy combined with the D1-MSC-siPLS calibration model is a feasible method for the quantitative analysis of biodiesel in adulterated hybrid fuels.

Enzymatic aqueous extraction with ultrasound: a prospective overview on the state of the art

The oil extracted from different oleaginous raw materials is usually obtained through mechanical pressing, associated or not with organic solvents. The objective of this prospection was to evaluate if the aqueous enzymatic extraction (AEE) with ultrasound should be a cleaner and more effective technology for the production of lipids and other products. The state of the art of this issue was observed with a focus on food, and more precisely oleaginous fruits and seeds. The advanced search in the Science Direct, Scopus and Web of Science (WOS) databases was carried out in July 2021. A total of 1,099, 79, and 46 scientific papers were retrieved within the Science Direct, WOS and Scopus databases, respectively. Identifying the experiments made in these studies with EAE and ultrasound, there was a selection of 24, 13 and 4 original articles in the investigated bases. The articles described that ultrasound used as pre-treatment or during AEE increased the yield of food lipid extraction. This prospection described that EAE technology and ultrasound are impactful techniques in obtaining lipids. The advanced research highlighted that there is a gap related to researches with oleaginous matrices and the replacement of conventional techniques with clean techniques can be economically feasible.

β-Carotene biofortification of chia sprouts with plant growth regulators

Chia (Salvia hispanica) is a native plant species from South America that is very appreciated for its oleaginous seeds in the agri-food field. Chia seeds are natural sources of many bioactive compounds which provide benefits to human health. Nevertheless, chia sprouts have better nutritional properties than seeds, such as antioxidants, essential amino acids, and phenolic compounds. Among all these beneficial compounds, β-carotene has not been studied in chia sprouts. β-carotene is a precursor of vitamin A, which contributes to maintaining our health status. In this study, to improve β-carotene content in chia sprouts, some plant growth regulators (abscisic acid, methyl jasmonate and methyl salicylate) were applied exogenously to germinating chia seeds. Gibberellins A4/A7 and cytokinin 6-benzyladenine (Promalin®) were also applied, combined with the other regulators, to antagonize a possible inhibition in the germination. Seeds were grown in darkness for 4 days, then seeds were exposed to a short light stimulus (30′) and finally to a continued light stimulus (48h). β-carotene, xanthophylls, chlorophylls, de-epoxidation status of xanthophyll cycle (DPS), germination rate, and sprouts fresh weight were analysed. The results show that sprouts treated with methyl salicylate in-creased 2,35 fold their β-carotene content when they were exposed to light for 30’+48h. Sprouts fresh weight and germination were not affected by methyl salicylate. Although more research is needed before industrial application, it is concluded that methyl salicylate can be used to improve β-carotene contents in chia sprouts.

Neurotoxicity mechanism of Ochratoxin A

Mycotoxins, such as Ochratoxins, are widely distributed in nature and are common contaminants of human foodstuffs. Ochratoxins are a group of mycotoxins produced by a wide range of molds. Ochratoxin A (OTA), the most prominent member of this toxin family, is produced by various Aspergillus and Penicillium species. OTA is frequently found in foods such as cereals, oleaginous seeds, coffee, and meat products. This mycotoxin has been described as teratogenic, genotoxic, carcinogenic, and immunotoxic, and has been proven to be a potent neurotoxin. In the present study, the neurotoxicological perspective of OTA was reviewed and discussed. The main possible mechanisms of neurotoxicity are oxidative DNA, protein and lipid damage, and apoptosis. However, further studies are needed to conclude the exact neurotoxicity mechanism of OTA and find the approaches that reduce the neurotoxicity induced by OTA.

Chemical composition of germinating Tunisian almond (Prunus Amygdalus Mill.) seedlings oil

Almonds (Prunus amygdalus) are a rich source of many essential nutrients. However, there is a lack of enough information on almond varieties' biochemical composition, especially at the germination stage. Therefore, this study was conducted to determine the chemical components of the germinating Tunisian almonds. The study included determining the content of oils, proteins, fatty acids, and triglycerides during germination. Results indicated that the oleaginous seeds are rich in oil (55 to 65% of the dry mass) and crude protein (21.825 mg/mL). The dominant polyunsaturated fatty acids are oleic and linoleic acids which represent 64.53% and 24.38%, respectively, while palmitic acid is the most dominant saturated fatty acid with 7.65% of the total fatty acids. Also, the primary molecular types of triglycerides detected by L.C. analysis are triolein (32.3%) and dioleolinolein (24.0%), followed by palmitodiolein (12.5%) and oleodilinolein (12.6%.). The physico-chemical properties study revealed that almond oil remains stable, thus preserving its quality and nutritional value, even during transition from dormancy to germination. On the other hand, we also detected the presence of a lipolytic activity which is maximum on the 3rd day of germination (4.66 mUI). Our results indicate that almond oil plays an important role in human nutrition due to the presence of unsaturated fatty acids, and it is more stable than other oils.

Ectopic overexpression of a type-II DGAT (CeDGAT2-2) derived from oil-rich tuber of Cyperus esculentus enhances accumulation of oil and oleic acid in tobacco leaves

BackgroundEngineering triacylglycerol (TAG) accumulation in vegetative tissues of non-food crops has become a promising way to meet our increasing demand for plant oils, especially the renewable production of biofuels. The most important target modified in this regard is diacylglycerol acyltransferase (DGAT) enzyme responsible for the final rate-limiting step in TAG biosynthesis. Cyperus esculentus is a unique plant largely accumulating oleic acid-enriched oil in its underground tubers. We speculated that DGAT derived from such oil-rich tubers could function more efficiently than that from oleaginous seeds in enhancing oil storage in vegetative tissues of tobacco, a high-yielding biomass crops.ResultsThree CeDGAT genes namely CeDGAT1, CeDGAT2-1 and CeDGAT2-2 were identified in C. esculentus by mining transcriptome of developing tubers. These CeDGATs were expressed in tissues tested, with CeDGAT1 highly in roots, CeDGAT2-1 abundantly in leaves, and CeDGAT2-2 predominantly in tubers. Notably, CeDGAT2-2 expression pattern was in accordance with oil dynamic accumulation during tuber development. Overexpression of CeDGAT2-2 functionally restored TAG biosynthesis in TAG-deficient yeast mutant H1246. Oleic acid level was significantly increased in CeDGAT2-2 transgenic yeast compared to the wild-type yeast and ScDGA1-expressed control under culture with and without feeding of exogenous fatty acids. Overexpressing CeDGAT2-2 in tobacco led to dramatic enhancements of leafy oil by 7.15- and 1.7-fold more compared to the wild-type control and plants expressing Arabidopsis seed-derived AtDGAT1. A substantial change in fatty acid composition was detected in leaves, with increase of oleic acid from 5.1% in the wild type to 31.33% in CeDGAT2-2-expressed tobacco and accompanied reduction of saturated fatty acids. Moreover, the elevated accumulation of oleic acid-enriched TAG in transgenic tobacco exhibited no significantly negative impact on other agronomic traits such as photosynthesis, growth rates and seed germination except for small decline of starch content.ConclusionsThe present data indicate that CeDGAT2-2 has a high enzyme activity to catalyze formation of TAG and a strong specificity for oleic acid-containing substrates, providing new insights into understanding oil biosynthesis mechanism in plant vegetative tissues. Overexpression of CeDGAT2-2 alone can significantly increase oleic acid-enriched oil accumulation in tobacco leaves without negative impact on other agronomy traits, showing CeDGAT2-2 as the desirable target gene in metabolic engineering to enrich oil and value-added lipids in high-biomass plants for commercial production of biofuel oils.

Condiciones de mercado para la demanda nacional del Sacha Inchi en Ecuador

One of the problems that the Latin American market faces is the scarcity of resources. The present research aims to analyze the conditions presented by the market for vegetable oil from oleaginous seeds, such as Sacha Inchi, as an alternative product to African palm oil. The methodology used is based on a quantitative approach with descriptive scopes, using a matrix of calculation of the dynamics of opening, closing and survival of companies, to verify which of the variables such as: Turbulence, gross entry, gross exit and net entry, incur as market conditions for the national demand for Sacha Inchi. The analysis of the sectoral dynamics makes it possible to measure levels of incursion of new companies into the seed agricultural sector, motivating microentrepreneurs to adhere to sacha inchi to their production. It is concluded that in the canton La Concordia 62.46% of companies enter the sector during the last 10 years with an average life of 8 years, being an attractive sector to introduce the seed of sacha inchi for the production of vegetable oil. Which would represent an alternative resource for the canton's main activity, such as the production and sowing of African palm.

EXPERIMENTAL INDUCTION OF TYPE 2 DIABETES MELLITUS AND THE EFFICIENCY OF BARIATRIC SURGERY IN ITS REVERSAL IN RATS.

Following recent years, there is an increased body of literature on the connections that might exist between type 2 diabetes mellitus and the efficiency of bariatric surgery in its reversal compared to other medical approaches such as dieting. To induce experimentally type 2 diabetes mellitus in rats in order to observe the effects of bariatric surgery in the recovery as well as the reestablishment of normal insulin levels in order to extend the findings in house animals. This study was conducted in three stages: the first consisted in inducing type 2 diabetes mellitus (T2DM) in 40 young Wistar male rats, by initially feeding them human food high in vegetal fats, oleaginous seeds, simple and complex carbohydrates, sugars, lipids, fats, proteins and fructose for a period of 8 weeks followed by a single low dose of streptozotocin (STZ), administered through intraperitoneal injection. The second stage of the study started when the rats became obese and therefore qualified for the bariatric procedure and the third stage consisted of post-operation supervision and care. The surgical procedure, performed on 10 obese rats, consisted in reducing the size of the stomach by partial gastrectomy of a 1.5 - 2.0 cm wide and 6.5 - 7.5 cm long area on the large curvature. Showed rapid improvements in body weight and blood sugar control after 9 days. After putting the rats on a diet high in carbohydrates, sugars, lipids and fats and administering them STZ, the induction of type 2 diabetes was successful and the partial gastrectomy led to a better blood sugar control. The bariatric procedure provides a faster therapeutic response than conventional diets.

Potential of some autochthonous wild plants of Burundi for vegetable oil and valuable compounds production.

Twelve species of indigenous plants have been studied in order to valorize some natural resources of Burundi (Eastern Africa) to investigate possibilities of vegetable oil production. Physicochemical properties and oil contents were determined from seeds harvested through five ecogeographic zones. From oilcake extracts, total sugars contents, proteins (TPrC), polyphenolic (TPhC), and flavonoids were quantified using spectrophotometry. Furthermore, antioxidant activity of oilcake extracts was assessed by 2, 2-diphenyl-b-picrylhydrazyl (DPPH) radical-scavenging and ferric reducing antioxidant power (FRAP) assays. All oil contents obtained were found to be quite similar to those of common oleaginous seeds. The two highest were found in Parinari curatellifolia (61.44 ± 4.81% Dry Matter) and Myrianthus arboreus (48.26 ± 5.96% DM). More than half of the species have shown TPrC ranging from 10 to 24% dry matter of oilcake (DM). Brachystegia longifolia was revealed exceptionally stronger antioxidant potential: effectiveness antiradical of 163.06 ± 26.29 mL/μg.min (DPPH assay) and reducing power of 2618.21 ± 161.22 GAE/100 g DM (FRAP assay). TPhC were positively correlated (p < 0.05) to the antioxidant activity. This pioneering work on these wild species highlight the potential for producing vegetable oil and valuable biomolecule sources likely for food, cosmetics, pharmacy and industry.

Evaluation of different conventional lipid extraction techniques’ efficiency in obtaining oil from oleaginous seeds

Vegetable oils present great economic importance as they can be used and marketed by different industries based on their properties. Currently, several techniques can be employed to extract oils from seeds. However, novel techniques are not easily applicable in any environment. Thus, this study aimed to evaluate the efficiency of simple techniques; Bligh and Dyer, Soxhlet, and acid hydrolysis, in extracting oil from canola, flaxseed, soybean, and sunflower seeds. The sum of fatty acids found in extracted oils indicated Bligh and Dyer as the most efficient method among those assessed. Furthermore, the lower content of polyunsaturated fatty acids quantified in oils obtained by Soxhlet or acid hydrolysis may result from oxidation triggered using high temperatures required by both methods. Different lipid extraction methodologies were compared for the determination of fatty acid content in seeds.

Chemical Composition of Pistacia lentiscus Seeds’ Oil from Moroccan High Atlas Mountain

Pistacia lentiscus is an aromatic member of the Anacardiaceae family, endemic to the Mediterranean regions, which produces red and black oleaginous seeds in maturity. Our study focuses on the characterization of Pistacia lentiscus seed oils from three localities of High Atlas Mountain in the Azilal region of Morocco. Our results showed a very appreciable oil yield reaching more than 21% which clearly differs between the three sites studied (21.33 ± 0.17% in Boizoghrane, 15.22 ± 0.10% in Tighanimine, and 7.67 ± 0.29% in Tawjanizm locality); the analysis of the total fatty acids composition revealed a predominance of the unsaturated fatty acids represented essentially by the oleic and linoleic fatty acids, and also the triglyceride composition was dominated mainly by POO + SOL, POL + SLL + PoOP, and OOO. This potential of the chemical composition offers the possibility of producing nutraceutical oil, which represents a source of income capable of insisting vulnerable local populations to protect this species from deforestation, thus maintaining biodiversity, and to reduce by this interest the migratory flow from marginal areas.

Oilseed Enzymatic Pretreatment for Efficient Oil Recovery in Biodiesel Production Industry: a Review

Biodiesel is an alternative to fossil diesel, non-toxic, and less pollutant. The production of biodiesel occurs with the use of oils, which are extracted from oleaginous seeds such as soybean, rapeseed, and palm fruit. The extraction of oils from oilseeds is one bottleneck of biodiesel production impacting on significant processes’ losses and productivity. This review analyzes different technologies to improve the oil extraction focusing on the enzyme-assisted aqueous extraction (EAAE) methods. EAAE is an environmentally friendly technology that takes advantage of the degradation efficiency of the enzymes, in this case hemicellulases preparations, which specifically degrade different structures that are present in vegetable cell walls to improve oil extraction from oilseeds. The enzymes used in this process are industrially produced and are highly efficient, but with high costs. The use of agro-industrial subproducts or the improvement of the enzymes’ producing strains could be an interesting solution for viable enzymatic preparations’ production to attend the biodiesel industries’ demand. EAAE treatment also shows great potential in techno-economic analysis allowing a faster recovery of profits and at a smaller production scale than the hexane extraction methodology.

Optimization of oleaginous seeds liquefaction using response surface methodology

Bio-oil production from sunflower seeds, as model components, was carried out in supercritical hydrothermal liquefaction conditions. The effects of operating parameters such as temperature, time, and biomass/solvent mass ratio were investigated. Response surface methodology based on full factorial design was utilized to optimize the operating conditions using Design Expert software. From the analysis of variance, the most influential factor of each experimental design response was identified and a regression model was derived. The results show that the quadratic polynomial model provided accurate predictions for bio-oil yield and its viscosity, with a determination coefficient R2 of 0.9120 and 0.9351, respectively. The optimum condition was 286.21 °C, a reaction time of 12 min, and 20% of biomass/solvent mass ratio. These conditions led to obtain 79.96 wt.% of bio-oil with a viscosity of 18.09 mPa s. The produced bio-oil was subjected to different analyses and characterized by gas chromatography–mass spectrometry. Besides, ether esters were identified as major components. Bio-oil properties were evaluated according to standard norms, and the results suggest the need of further upgrading step to improve its quality.

Sacha inchi (Plukenetia volubilis L.)-from lost crop of the Incas to part of the solution to global challenges?

The underutilized, oleaginous crop Plukenetia volubilis L. has a remarkable lipid composition and a large potential for further domestication, alleviation of malnutrition, and integration into sustainable food production systems. Current global challenges include climate change, increasing population size, lack of food security, malnutrition, and degradation of arable lands. In this context, a reformation of our food production systems is imperative. Underutilized crops, or orphan crops, can provide valuable traits for this purpose, e.g., climate change resilience, nutritional benefits, cultivability on marginal lands, and improvement of income opportunities for smallholders. Plukenetia volubilis L. (Euphorbiaceae)-sacha inchi-is a 'lost crop' of the Incas native to the Amazon basin. Its oleaginous seeds are large, with a high content of ω-3, and -6 fatty acids (ca. 50.5, and 34.1%, of the lipid fraction, respectively), protein, and antioxidants. Culinarily, the seeds are nut-like and the crop has been associated with humans since Incan times. Research has particularly been undertaken in seed biochemistry, and to some extent in phylogeny, genetics, and cultivation ecology, but attention has been unevenly distributed, causing knowledge gaps in areas such as ethnobotany, allergenicity, and sustainable cultivation practices. Recently, seed size evolution and molecular drivers of the fatty acid synthesis and composition have been studied, however, further research into the lipid biosynthesis is desirable. Targeted breeding has not been undertaken but might be especially relevant for yield, sensory qualities, and cultivation with low environmental impact. Similarly, studies of integration into sustainable management systems are of highest importance. Here, present knowledge on P. volubilis is reviewed and a general framework for conducting research on underutilized crops with the aim of integration into sustainable food production systems is presented.

Diversity of Lipase-Producing Microorganisms from Tropical Oilseeds Elaeis guineensis, Ricinus communis, and Jatropha curcas L. from Costa Rica.

Tropical oleaginous seeds are an unexplored source for the discovery of novel lipolytic microorganisms, which could be applied to the bioremediation of agro-industrial oily wastes and solve numerous environmental issues. Such wastes hold potential to be revalorized towards a variety of products through microbial bioremediation. In this study, we investigate the microbial diversity and lipase activity from bacterial and fungal isolates obtained from the oil seeds of Elaeis guineensis, Ricinus communis, and Jatropha curcas L. from Costa Rica. A total of 27 strains were confirmed as lipase-producing strains via fluorogenic and colorimetric agar plate assays. The diversity of the isolates comprises 12 fungal ascomycetes from the genera Aspergillus and Fusarium and 15 bacterial isolates classified into four genera: Serratia, Proteus, Pseudomonas, and Bacillus. Microbial isolates from E. guineensis showed the highest diversity of lipolytic microorganisms (6 genera) followed by J. curcas (4 genera) and R. communis (2 genera). Isolates showing the highest activity in agar plates were tested further by submerged fermentation and the specific lipase activity was measured with 4-nitrophenyl laurate as substrate. Accordingly, the highest specific lipase activity was demonstrated by Bacillus pumilus B5 (24.98Umg-1), Serratia marcescens B10 (17.65Umg-1), Pseudomonas mendocina B16 (8.62Umg-1), and Bacillus pumilus B1 (5.72Umg-1) in submerged fermentation. These findings indicate the presence of a specialized microbial diversity in tropical oil seeds and highlight their potential to be applied in the bioremediation of agro-industrial oily wastes.

Extraction of camelina mucilage with ultrasound and high flow rate fluid circulation

As the outermost layer of many oleaginous seeds, mucilage can be extracted by different shear forces. At lab scale, ultrasound is an effective extraction method and is associated here with hydrodynamic forces on a higher scale. In the example of camelina mucilage, an ultrasonic tubular reactor and a recirculating pump are used separately and combined with a response surface methodology. With a seed concentration of 10 % in water, mucilage extraction yields of 6.9 ± 1.5 % and 6.6 ± 0.3 % are obtained after 55’ of pulsed ultrasonic treatment with a 3 L ultrasonic tubular reactor and a lab scale (100 mL) ultrasonic probe, respectively. High flow rate recirculation (100 L/min) used alone allows yields of more than 10 %. Extraction efficiency then depends directly on the rheological behavior of the medium and allows a high seed concentration (up to 15 %). The combination of ultrasounds and high flow rate circulation reveals a high importance of ultrasound on protein composition and rheological behavior of mucilage while extraction yield, protein and ash content are mainly controlled by circulation time.

Proteomic identification of lipid-bodies-associated proteins in maize seeds

Seeds store lipids in the form of lipid bodies (LBs) for germination and early seedling growth. LBs can be easily isolated by the established floating-extraction method from oleaginous seeds containing a large quantity of LBs. Compared to oleaginous seeds, maize and other cereal seeds contain a small quantity of LBs, so it is difficult to isolate a sufficient quantity of LBs from their embryos for 2DE-based proteomic analysis. At present, only a limited number of LBs-associated proteins in maize embryos have been identified. We here reported a modified floating-extraction method using polyvinylidene difluoride disc to collect floating LBs from maize embryo extracts. The LBs-associated proteins were resolved with two-dimensional electrophoresis and identified with mass spectrometry. As a result, several well-known LBs proteins were identified in the purified LBs fraction, such as oleosin, caleosin, and steroleosin. We also identified another two LBs proteins, corticosteroid 11-β-dehydrogenase 1 and 11-β-hydroxysteroid dehydrogenase-like 5. In particular, steroleosin, corticosteroid 11-β-dehydrogenase 1, 11-β-hydroxysteroid dehydrogenase-like, and hydroxyproline-rich glycoprotein were found as the most abundant protein components in maize LBs. The data set of maize LBs subproteome would provide insights into functional research of LBs-associated proteins during seed development and germination. Additionally, the protocol developed here is expected to be applicable for isolating LBs in other seeds or tissues containing a low quantity of LBs.

Researches on obtaining vegetable oils as a source of alternative energy

Biodiesel type fuel is widely used on a large scale in most of the European Union. Carbon dioxide is eliminated by combusting biodiesel, but not toxic emissions. Due to the more drastic European ecological demands to reduce the quantity of toxic emissions from automobiles, there is an increased interest to use biodiesel, because it is a bio-degradable and non-polluting fuel. Even by mixing diesel fuel with biodiesel in a percentage of 20%, the emission of toxic gases will be greatly reduced. Vegetable oils used as biofuels are obtained from oleaginous plant crops, without any prejudice to food security. The use of biodiesel can also bring benefits by prolonging the lifetime of diesel engines, ensuring a better lubrication. The paper presents a series of researches on obtaining vegetable oils from various oleaginous seeds (rapeseed, camelina, hemp) using a special installation for producing oils through cold pressing.