Oily Extract Research Articles

Insecticidal Effect of Two Chemotypes of the Essential Oil of Eucalyptus camaldulensis Dehnh against Aphis fabae Scopoli

Background: The objective of this study was to study the insecticidal and repellent effect of the essential oil of the plant Eucalyptus camaldulensis Red Dehnh extracted by hydrodistillation method planted in the Ouargla region against the black aphid. Methods: The insecticidal effect of essential oils against aphid mortality by the introduction of bean leaflets in the different oily extracts with different concentrations and the count of dead individuals after 3, 6 and 24 h at the physiology laboratory in Ghardaïa University. Result: Through this study, the leaves treated with the major compounds of Eucalyptus camaldulensis essential oil such as Benzene,1-methyl-4-(1-methylethyl) at 35 per cent concentration after 24 hours, a 65 per cent aphid mortality was recorded which is more effective compared to Sabinene and essential oils of Eucalyptus camaldulensis at different concentrations (5; 35%; and 50 per cent). As for the repellent effect is 87 per cent after 6 hours for the pure essential oil of E. camaldulensis.

The therapeutic effect of the oily extract of Cannabis sp. in Aluminum Chloride-Induced Alzheimer's Disease in Rats

Objective: To evaluate the therapeutic potential of Cannabis sp extract in a model of Alzheimer's disease induced by aluminum chloride in rats. Methods: Thirty Wistar rats were divided into five groups (n=6) where one group received no treatment, another group received only aluminum chloride and the others, in addition to aluminum chloride, received different doses (50, 100 and 150µl) of oily extract of Cannabis sp. The animals were euthanized one day after treatment, where the brain was collected, fixed, processed and embedded in paraffin. Histological sections of the hippocampus were used for qualitative and quantitative assessment of morphological changes. Blood serum was also obtained to determine indicators of oxidative status. Results: Reduction of more than 80% in cell death in the dentate gyrus region of the hippocampus in animals treated with cannabis extract. Furthermore, a reduction in total protein levels by approximately 20% and an increase (15%) in antioxidant enzymes in relation to the positive control were also observed in the serum. Conclusion: The use of the oily extract of Cannabis sp. in a 2:1 ratio of delta-9-tetrahydrocannabinol (THC): Cannabidiol (CBD) effectively reduced hippocampal neuron deaths from amyloidosis induced in chronic aluminum chloride intoxication.

Skin- and Eco-Friendly Hand Sanitizer: A Novel Composition of Natural Extracts to Prevent the Spread of Respiratory Viruses.

Respiratory diseases caused by viruses are a serious global health threat. Although the use of hand sanitizers containing alcohol and synthetic antiseptic agents is recognized as an effective, simple, and low-cost measure to combat viral transmission, they can harm human health and the environment. Thus, this work aimed to study the efficacy of combining Camellia sinensis and Chamomilla recutita extracts in a skin- and eco-friendly leave-on hand sanitizer to prevent the spread of respiratory viruses. An oil-in-water emulsion containing C. recutita oily extract (5.0%), C. recutita glycolic extract (0.2%) and C. sinensis glycolic extract (5.0%) showed virucidal activity against HAdV-2 (respiratory virus) and two surrogate viruses of SARS-CoV-2 (HSV-1 and MVH-3), showing great potential to prevent the spread of respiratory viruses. These natural extracts combined are also promising to combat a broad spectrum of other viruses, in the form of antiseptic mouthwashes or throat sprays, surface disinfectants, and veterinary products, among others. Complementally, the developed hand sanitizer demonstrated efficacy against bacteria and fungus.

Supercritical CO2 extraction of carotenoids from high Andean forest bee pollen

An oily bee pollen extract was obtained using the supercritical fluid technique (SFE) using CO2 as a solvent and conditions of pressure: 28 MPa, temperature: 60°C, gas flow: 5 L/min, and duration: 6 h. Initially, pollen samples were collected from different municipalities of Colombia, selected, and analyzed according to the content of total carotenoids (CCT) and yield of the extraction process. The results show CCT between 119 and 2140 µg β-carotene/g of sample, with yield percentages in obtaining oily extracts between 3 – 5%. For CCT in the oily extract, 385–6942 µg β-carotene/g of extract were obtained, where Guatavita pollen was the one with the highest content. According to the investigation, the authors derived that the technique of SFE positively affected the exine layer, shown in the observations by Scanning Electron Microscopy (SEM).

A New Method for Selective Extraction of Torularhodin from Red Yeast Using CO2-SFE Technique.

Torularhodin is a dark pink colored carotenoid belonging to the xanthophylls group that can be biologically synthesized by red yeasts, especially by Rhodotorula and Sporobolomyces genera. The growing interest in this molecule is due to its biological activities such as antioxidant, anticholesterolemic, anti-inflammatory, antimicrobial, and anticancer. To satisfy potential commercial markets, numerous methods have been proposed to develop a cost-effective and environmentally friendly downstream process for the purification of torularhodin. However, obtaining high purity products without resorting to the use of toxic solvents, which can leave residues in the final preparations, remains a major challenge. In this context, the present study aimed to develop a new efficient method for the isolation of torularhodin from the red yeast Rhodotorula strain ELP2022 by applying the extraction technique with supercritical CO2 (CO2-SFE) in two sequential steps. In particular, in the first step, the dried lysed biomass of yeast was subjected to the action of CO2 in supercritical conditions (CO2SC) as sole solvent for extraction of apolar carotenoids. In the second step, the residual biomass was subjected to the action of CO2SC using ethanol as a polar co-solvent for the extraction of torularhodin. Both steps were carried out at different operating parameters of temperature (40 and 60 °C) and pressure (from 300 to 500 bar) with a constant CO2 flow of 6 L min-1. Regardless of the operating conditions used, this method allowed to obtain an orange-colored oily extract and a red-colored extract after the first and second step, respectively. In all trials, torularhodin represented no less than 95.2% ± 0.70 of the total carotenoids in the red extracts obtained from the second step. In particular, the best results were obtained by performing both steps at 40 °C and 300 bar, and the maximum percentage of torularhodin achieved was 97.9% ± 0.88. Since there are no data on the selective recovery of torularhodin from red yeast using the SFE technique, this study may be a good starting point to optimize and support the development of industrial production of torularhodin by microbial synthesis. This new method can significantly reduce the environmental impact of torularhodin recovery and can be considered an innovation for which an Italian patent application has been filed. In a circular bioeconomy approach, this method will be validated up to a pilot scale, culturing the strain Rhodotorula spp. ELP2022 on low-cost media derived from agri-food wastes.

Innovative Multilayer Electrospun Patches for the Slow Release of Natural Oily Extracts as Dressings to Boost Wound Healing.

Electrospinning is an advanced manufacturing strategy used to create innovative medical devices from continuous nanoscale fibers that is endowed with tunable biological, chemical, and physical properties. Innovative medical patches manufactured entirely by electrospinning are discussed in this paper, using a specific plant-derived formulation "1 Primary Wound Dressing©" (1-PWD) as an active pharmaceutical ingredient (API). 1-PWD is composed of neem oil (Azadirachta indica A. Juss.) and the oily extracts of Hypericum perforatum (L.) flowers, according to the formulation patented by the ENEA of proven therapeutic efficacy as wound dressings. The goal of this work is to encapsulate this API and demonstrate that its slow release from an engineered electrospun patch can increase the therapeutic efficacy for wound healing. The prototyped patch is a three-layer core-shell membrane, with a core made of fibers from a 1-PWD-PEO blend, enveloped within two external layers made of medical-grade polycaprolactone (PCL), ensuring mechanical strength and integrity during manipulation. The system was characterized via electron microscopy (SEM) and chemical and contact angle tests. The encapsulation, release, and efficacy of the API were confirmed by FTIR and LC-HRMS and were validated via in vitro toxicology and scratch assays.

Effect of Chemical Degradation of Sodium Alginate on Capsaicin Encapsulation

Capsaicin is known as an oily extract of paprika that is characterized by pungent taste and bioactivity. It also may cause irritation to the mouth and stomach which is why is so important to immobilize capsaicin on a carrier to prevent it. The usage of alginate oligomers, which has an antioxidant potential compared to alginate, is of benefit because it may be used in the immobilization of bioactive substances that are fragile to oxidation. The purpose of this study was to use sodium alginate oligomers as a coating material in the encapsulation process of paprika oleoresin. Alginate oligomers were produced by chemical degradation with hydrogen peroxide. The characteristics of the samples were obtained by measuring the viscosity, the contact angle of the surface, and the surface tension of solutions. The obtained solution of alginate oligomers served as the carrier material for the immobilization of capsaicin. Capsules were prepared by ionic gelation using a calcium chloride solution as a crosslinking agent. In this way, capsules without and with the core (capsaicin) were prepared and their ability to scavenge free radicals (DPPH) and iron-reducing properties (FRAP) were determined. The stability of the capsules was examined by thermal decomposition and under conditions of the gastric and small intestine, and capsaicin content was detected using high-performance liquid chromatography. It was found that alginate oligomers could be used in the encapsulation of bioactive compounds and the efficiency was above 80%. Capsule production from alginate oligomers affected their thermal stability. The use of alginate derivatives as a carrier increased the antioxidant properties of the finished product, as well as its ability to reduce iron ions. The use of alginate oligomers as a coating material prevented the active substance from being released too early in the conditions of the small intestine, prolonged the stability of the capsules, and supported their durability in gastric conditions.

The effects of propolis on doxorubicin-induced hepatorenal damage: a comparison of ethanolic and oily extracts of propolis

ABSTRACT The protective effect of ethanolic propolis extract (PE) in the toxic effects of doxorubicin (DOX), a chemotherapy drug, has been reported previously. Since ethanol usage has some restrictions, in the present study, olive oil as an alternative to ethanol has been tested in DOX-induced hepatorenal injury and the effectiveness of the two extracts were compared. Male rats were orally given PE or oily propolis extract (PO) for 15 days (50 mg/kg/day) and DOX was injected (15 mg/kg, intraperitoneally) on the 13th day. The animals were sacrificed and then hepatorenal oxidative stress and functional parameters were evaluated. DOX-induced reduction in hepatorenal functions and oxidative stress were significantly reduced in both propolis treatment groups. Treatment with PO was more effective in reducing DOX-induced oxidative stress, yet both extracts showed similar efficacy in improving hepatorenal functions. We suggest that the PO could be preferred to avoid the side effects of ethanol.

A new prototype reactor for the fast microwave-assisted decarboxylation and extraction of cannabinoids in olive oil from Cannabis inflorescences

Several different Cannabis-based pharmaceutical preparations have been approved for medical treatments. Standardization to ensure specified phytocannabinoid contents and to maintain the integrity of the product is therefore required. Olive oil is clearly a safe and edible solvent suitable for the galenic preparation of ready-to-use Cannabis extracts. Pharmacists can use several non-standard procedures for this purpose, most of which involve the heat treatment of the plant matrix (i.e. the female inflorescences) for 2 h. In order to allow the “neutral” and more biologically active forms of the major phytocannabinoids to be extracted, prior thermal treatment of the plant matrix is desirable. This work investigates a new prototype microwave (MW) reactor (Ethos Lean) specifically designed for the decarboxylation of the acidic cannabinoids in Cannabis inflorescences and their subsequent extraction into oil. Both of the steps to produce the oily extract were carried out in the Ethos Lean's cavity with special accessories; a rotating drum for the decarboxylation process and a glass reactor with a stirrer for the extraction step. The variables considered in the process optimization were time and temperature, according to which the instrument automatically calibrated the power output. In collaboration with a hospital pharmacy, the efficiency of the device has been evaluated by comparing the results obtained with those of exhaustive decarboxylation in a conventional oven and ethanol extraction. A comparison was also made with conventional procedures in olive oil. Thanks to the rotating drum, which is sensitive to dielectric heating, the complete and homogeneous decarboxylation of phytocannabinoids was rapidly achieved (30 min, 120 °C), even with discrete quantities of inflorescences (150 g) and without releasing the characteristic intense odor into the laboratory. The exhaustiveness (100% yields of both CBD and THC) of MW-assisted extraction process in olive oil was achieved with 30 min irradiation at 90 °C. Furthermore, the possible decarboxylation occurring during MW-assisted extraction in oil and the effect of cooling time on the final yields have also been evaluated.

Bioactive Fractions Isolated from By-Products of the Guava (Psidium guajava) and Mango (Mangifera indica L.) Agri-Food Industry

Valorizing agri-food industrial waste is essential for a circular economy, yielding high-value products, waste reduction, technological solutions, employment opportunities, and enhanced food security. This work shows the valorization of seeds generated as residues from the agri-food industries of guava pera (Psidium guajava) and Tommy Atkins mango (Mangifera indica L.), through extraction with supercritical carbon dioxide (scCO2). After the optimization of the initial solid condition of the raw material (i.e., particle size and moisture content), scCO2 pressure and temperature were established through the response surface methodology (RSM) to obtain an oily extract with the highest content in bioactive compounds of commercial relevance, as well as with a high antioxidant capacity. The total amount of oily extract in guava and mango seeds was 14% and 9%, respectively, while the maximum recovery of supercritical extract was 95% from guava seeds at 38 MPa and 50 °C, and 88% from mango seeds at 37 MPa and 63 °C. Bioactive fractions rich in squalene, γ-tocopherol, α-tocopherol, campesterol, β-sitosterol, and stigmasterol were obtained. The best supercritical extraction conditions, in terms of the bioactive fractions richest in minor compounds, were at 17 MPa and 50 °C for guava seeds and at 23 MPa and 63 °C for mango seeds. At these conditions, the highest antioxidant capacities were also found for the extracts. Thus, these bioactive fractions could be used in a variety of products in the cosmetic, food, pharmaceutical, and medical activities due to the beneficial properties of the identified compounds in health as antioxidants, anti-inflammatories, and cholesterol reducers.

Evaluation Of Antibacterial Activity In Pelargonium Graveolens Extracts

The antibacterial activity of the total ethanolic extract and fractions of Pelargonium graveolens against Candida albicans, Escherichia coli, Pseudomonas spp, Staphylococcus aureus and Bacillus spp. Methods: To measure the relative antimicrobial activity, the percentage of inhibition was calculated by the plate and well method. Results: It was established that the highest antimicrobial activity was presented by the oily extract without preference for Gram positive or Gram negative, the acetone fraction with a value of 58.53% inhibition. The hexane fraction has activity greater than 100% only against S. aureus. Conclusions: The extracts of P. graveolens present antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts, especially the oily extract, which presented the highest percentage of inhibition recorded against Escherichia coli, Staphylococcus aureus and Candida albicans, respectively; while for Pseudomonas aeruginosa and Bacillus subtilis, the extract that presented the highest percentage of inhibition was ethyl acetate.

TOXICITY OF Argemone mexicana (L.) EXTRACTS AND IMIDACLOPRID ON Chrysoperla carnea (Stephens)

In sorghum (Sorghum bicolor L. Moench) cultivation, the main pest is the sugarcane aphid (Melanaphis sacchari Zehntner (Hemiptera: Aphididae)). The insecticide imidacloprid is frequently used to control this insect, and in some cases, it is complemented with the release of lacewings of the species Chrysoperla carnea Stephens (Neuroptera: Chrysopidae). The insecticide has been shown to be harmful to non-target insects, such as the natural enemies of this aphid, so it is necessary to find other less harmful products. The objective of this study was to evaluate the toxicity of aqueous and oily extracts of chicalote (Argemone mexicana L.) on C. carnea and to contrast it with that of imidacloprid, with the hypothesis that extracts of A. mexicana applied to the developmental stages of C. carnea generate less mortality on the predator than imidacloprid. The aqueous and oily extracts had a concentration of 30 g L-1. For imidacloprid, the recommended dose for sugarcane aphid control (0.35 g L-1) was used. Mortality and the treatments effect were evaluated by exposure methods: T1) topical on larvae, T2) egg immersion, T3) treated Sitotroga cerealella eggs, and T4) liquid ingestion in adults. Mortality, hatching and emergence variables were analyzed with a one-way ANOVA, followed by Tukey’s comparison of means (p ≤ 0.05). Treatments were classified according to the toxicity levels proposed by the International Organization for Biological Control. The aqueous and oily extracts were in toxicity class 1 for C. carnea, except in eggs treated with the oily extract (class 4). Imidacloprid was classified in class 4 when applied topically and by ingestion in adults and in class 2 in ingestion of treated S. cerealella eggs. In general, extracts of A. mexicana could be used as selective insecticides, while imidacloprid can be considered incompatible with releases of this predator.

Metabolomic Analysis and Antioxidant Potential of Tropical Propolis Nonpolar Extracts from Colombia

Bioactive compounds of natural matrices are of interest because of their application in the food and pharmaceutical industries. One important source of bioactives is propolis, a resinous biomass that bees make from the plants surrounding the hive. Regarding Colombian propolis, studies have mostly been directed towards identifying flavonoids and their biological activity in vitro. There are no works on the oil extract of propolis and its chemical composition and bioactivity through metabolomic analysis. In this sense, this work studied the volatile composition of propolis oil extract samples from Colombia, following a metabolomic approach; in this work, the oily Colombian propolis extracts (OECP) obtained by an ultrasound assisted extraction (UAE) with different solvents were studied using spectroscopy and physicochemical analysis. A strong correlation was observed between the antioxidant activity and their concentrations of polyphenols, terpenoids, and carotenoids. Simultaneously, it was possible to expand the number of compounds identified by applying FTIR spectroscopy to the sample analysis, relating infrared bands to specific compounds. Besides, it was also possible to discriminate the samples according to their geographical origin. Colombian propolis oil extracts have characteristics as bioactive compounds such as the sesquiterpenes bisabolol, α-zingiberene, β-bisabolene, and α-trans-bergamotene and the monoterpenes α-pinene, linalool, and p-cymene.

Investigation on the Composition and Extraction Mechanism of the Soluble Species from Oily Sludge by Solvent Extraction.

Oily sludge (OS) was extracted with petroleum ether (PE), methanol, carbon disulfide (CDS), acetone, and isometric CDS/acetone mixture (IMCDSAM), respectively, to obtain soluble species (E1-E5) and extraction residues (R1-R5). The soluble species were analyzed by gas chromatography/mass spectrometry (GC/MS), and the extraction residues were characterized by Fourier transform infrared spectrometry (FTIR) and thermogravimetric analysis (TGA). Results showed that the extract yield of the soluble species from OS using CDS and IMCDSAM as the solvent was 61.0 and 67.3%, respectively. GC/MS results exhibited that the compounds detected in E1-E5 are mainly hydrocarbons and oxygen-containing compounds. E1-E5 are rich in alkanes, alkenes, ketones, alcohols, and other oxygen-containing compounds. Double-bond equivalence (DBE) and carbon numbers (CNs) of the compounds detected in E1, E2, and E4 are distributed in 0-4 (DBE) and 10-20 (CNs), respectively, while the DBE and CNs of the detected compounds in E3 and E5 are concentrated in 0-6 and 15-35, respectively. Thermogravimetry-differential thermogravimetry (TG-DTG) profiles presented that pyrolysis of OS occurred mainly in the temperature range of 150-750 °C, while pyrolysis of R1-R5 took place in the range of 350-750 °C. In the temperature range of 150-550 °C, the weight losses of OS and each extraction residue differ significantly, with OS having a much higher weight loss than the extraction residues. Meanwhile, the possible mechanism of oily sludge extraction was considered. Results revealed that selecting a low-polar or nonpolar solvent capable of selectively destroying hydrogen bonds and/or aromatic interactions is critical for improving the extract yield of OS.

1463 Upcycled fraction of melaleuca alternifolia oily extract to regenerate the skin through the skin melatonin pathway.

1463 Upcycled fraction of melaleuca alternifolia oily extract to regenerate the skin through the skin melatonin pathway.

Coupling in-syringe kapok fiber-supported liquid-phase microextraction with flow injection-mass spectrometry for rapid and green biofluid analysis: Determination of antidepressants as an example

Quantification of substances in biofluid samples (e.g., urine, blood, and cerebrospinal fluids) are useful for clinical diagnosis. In current study, a rapid and green strategy by coupling in-syringe kapok fiber-supported liquid-phase microextraction with flow-injection mass spectrometry was proposed. The natural kapok fiber was used as an oily extraction solvent (e.g., n-octanol) support material, and an in-syringe extraction device was conveniently constructed. The whole extraction processes, including sampling, washing and desorption, were conveniently conducted by simply pulling/pushing the syringe plunger, enabling rapid analyte enrichment and sample purification. The follow-up flow injection-mass spectrometry detection enabled rapid and high throughput analysis. As an example, the proposed method was applied to analyze antidepressants in plasma/urine, showing satisfied linearities (R2 ≥0.993) in ranges of 0.2–1000 ng/mL. By employing the in-syringe extraction method prior to flow injection-mass spectrometry detection, the LOQs in plasma and urine were reduced by 25–80 folds and 5–25 folds, respectively. Besides, by employing ethanol and 80% ethanol as the desorption solvent and carrier solvent, respectively, the analytical method showed excellent greenness. In general, the integrated method provides a promising choice for rapid and green biofluid analysis.

Correction: Surface treatment and corrosion protection of mild steel by the oily extracts of olive mill waste cake

Correction: Surface treatment and corrosion protection of mild steel by the oily extracts of olive mill waste cake

Surface treatment and corrosion protection of mild steel by the oily extracts of olive mill waste cake

Surface treatment and corrosion protection of mild steel by the oily extracts of olive mill waste cake

Total carotenoid content of Padina sp. seaweed-infused oil with virgin coconut oil

Padina sp. seaweed is a species of brown seaweed that grows naturally in Indonesian waters and it is not been optimally utilized. Padina sp. contains bioactive natural carotenoids that can apply as additive ingredients in functional foods and cosmetics field. This study aimed to determine the total carotenoid content in Padina sp. seaweed-infused oil. Seaweed oily extract or Padina sp. seaweed-infused oil was obtained from the filtered maceration of Padina sp. powder using virgin coconut oil as a solvent. Furthermore, the total carotenoid content in Padina sp. seaweed-infused oil, Padina sp. powder and virgin coconut oil as solvent for maceration of Padina sp. were analyzed using the spectrophotometry method. Padina sp. powder and virgin coconut oil without Padina sp. (as control) contained total carotenoid content 2.42±0.32 µg/g and 0.71±0.66 µg/g respectively. Padina sp. seaweed-infused oil had total carotenoid content by maceration process with virgin coconut oil as solvent at a temperature of 65 °C for 1 hour, 3 hours, and 5 hours were 2.73±0.36, 2.18±1.01, and 1.74±1.24 µg/g respectively. Therefore, the total carotenoid content increased in Padina sp. seaweed-infused oil. It could be an opportunity for functional food products and cosmetic ingredients.

In vitro: anticancer effect of oily and methanolic extracts of Al-Zahdi (Phoenix dactylifera L.) from dry dates and leaves on AMN3, Hela and Ref cancer cell cultures

The antioxidant and anti-tumor effects of extracts from various herbs and medicinal plants were measured using various in vitro and in vivo methods. The current study investigates and compare the anticancer effects of the two palm part extracts against cancer cell line in cultured cell line of AMN3, Hela and Ref. The results showed a concentration dependent inhibitory cytotoxic effects during 72 hrs of exposure for methanolic and oily crude extracts of date and leaves on AMN3, Hela and Ref cell lines. The highest significant effect of both dry date and leave methanolic extracts were achieved at concentration 2500 μg/ ml that causing highest growth inhibition percentage (GIP) of (73.3 %, 66.4%) for AMN3 and (76.7 %, 55.4%) for Hela respectively. The concentration 5000 μg/ ml showed nearly less effect after 72 hrs exposure. Both methanolic extracts tested concentrations didn’t cause any inhibitory effect on the REF cell line. The effect of date and leaves oily extracts at different concentrations caused on tumor Cell Line AMN3, Hela and Ref highly significant in difference at exposure periods 72 hrs for concentrations gradient ranged from (15.62- 250 μg/ml). The concentration 125 µg/ml showed higher inhibition percentage compared to the higher concentration of 2500 µg/ ml causing on AMN3 (85.1%, 66%), Hela (79.1%, 77%), and Ref (76%, 61.7%), respectively. Plateau were noticed for all highest palm extracts concentrations GIP effect on all cell line cultures. The study concluded that the superiority of oil extract for both date and leave over alcoholic extracts one in inhibition of in vitro the same cell lines AMN3, Hela, Ref.