Oils Of Plant Origin Research Articles

Local Content Utilization and Product Quality Standards Promotion for Industrial and Machine Tools Processes Application

The research titled, local contents utilization and product quality standards promotion for industrial and machine tools processes application used oils of plant origin; Mahogany Seed Oil (MSO) and Cashew Nut Oil (CNO) to produce cutting fluids. These oils were used as base oils to formulate cutting fluids and were used in straight turning of low carbon steel through factorial design of experiment. Their performances in terms of resulting surface roughness (Ra) of machined samples) and heat dissipation tendency was evaluated. Also, the determination of some characteristics of the formulated cutting fluids was carried out. The two vegetable oils extracted were mixed with water in an appropriate ratio separately. The oil – water mixtures were finally blended with the necessary additives to obtain the various cutting fluids. Before machining, the flash points, viscosities and acidities of the cutting fluids were determined. During machining, the tool – work interface temperatures were recorded using digital thermocouple and the Ra of the machined samples was evaluated afterward using scanning electron microscope (SEM) The performances and some characteristics of the formulated cutting fluids were compared with that of a control sample (CS). The heat removal ability was best achieved by MSOCF being locally available vegetable oil-based cutting fluid attaining a temperature of 32oC at the highest cutting speed of 80mpm. Similarly, CNOCF produced the best surface roughness which range between 1.0675µm and 1.5715µm for all levels of cutting speeds. Besides the same MSOCF proved to be the safest from fire hazard with the highest flash point of 237oC. CNOCF has the highest viscosity at 40oC and 80oC being 134cp and 96cp. MSOCF was found to be the best among the cutting fluids in conducting heat away from tool-work piece interface and was followed by CNOCF. From the foregoing, it can be concluded that locally available vegetable oils can be used in the production of cutting fluids for machining operations. Hence, the vegetable oils cutting fluids can then conveniently substitute the conventional (mineral oil based) cutting fluids for all machining processes and operations under various operating conditions as coolants and lubricants. They could also be synergized to compete favorably with mineral based cutting fluids.

Методика оптико-электрохимического микробиологического тестирования в применении к сравнительному анализу свойств эфирных масел

Introduction. The national economy is currently developing a system for monitoring the quality and safety of goods. Food products, ingredients, and additives possess various pro- and antibiotic properties. Therefore, available express methods of quantitative assessment of microbiological contamination are a relevant aspect of domestic food industry.
 Study objects and methods. The study featured ten essential oils of plant origin that can be used as functional additives to various food products.
 Results and its discussion. The research introduced a new biotesting technique for repetitive recording of changes in the intensity of elastic light dispersion. The technique made it possible to measure pH and electrical conductivity of a liquid nutrient medium incubated in the presence and absence of viable test microorganisms and test samples. The paper describes the results of this technique applied to a comparative analysis of antibiotic activity of various essential oils in different concentrations. As the concentrations of the test extracts decreased, their antibiotic activity monotonically also went down, while the probiotic activity increased. The shortterm biological activity of test samples appeared to be significantly higher than their long-term activity. The medium-term biological activity of the test samples was mostly intermediate in value. Only rarely did it exceed both the long- and short-term biological activity of the same TE. The essential oils obtained from the leaves of Thuja occidentalis, Eucalyptus globulus, and Cupressus sempervirens exhibited the most active and long antibiotic properties.
 Conclusion. The biological activity of food products, including various plant extracts, depends not only on the raw material and the extraction method, but also on the concentration of the extract in the product. As a rule, the exact nature of these dependencies can only be established empirically and requires a set of various tests. The present article introduces a new highly objective and informative express methodology that simplifies this process. The technique is less labor-, time-, and material-consuming than standard visual microbiological methods. It can be used to assess the effect of test samples on the vital activity of microorganisms in various foods, ingredients, and additives.

Immuno-Potentiator Effect Of Rapeseed Or Soybean Oil With Foot And Mouth Disease Vaccine In Calves

One effective recommendation for control of Foot and Mouth disease virus is the proper vaccination by a highly potent vaccine. Such a vaccine should be specific to the circulated field serotype of foot and mouth disease virus inducing rapid, highly protective immunity with a long duration. This research is concerned with using plant extracted oil adjuvants prepared from rapeseed oil mixed with or without Ginseng extracts and from Soybean oil as well with or without Ginseng extracts. Some humeral and cell-mediated immune responses were compared using the different plant-extracted oils cocktails and the mineral-based adjuvant called Montanide oil ISA 206 after one dose vaccination of the prepared vaccines at different interval times post vaccinations. Lymphocyte blastogenesis, Interleukin -6, and Interleukin-12 showed higher expression in calves vaccinated by FMDV serotypes (O pan Asia, A Iran O5, and SAT2 / EGY/2012) with plant cocktails containing Ginseng extract comparable to that of plant extracts without Ginseng and Montanide oil ISA 206 adjuvants vaccines. It was found that FMD vaccine adjuvant with ginseng either with rapeseed or Soybean showed a higher post vaccinal cellular immune response than that without ginseng or ISA 206 alone. Estimation of the humoral immune response of vaccinated calves revealed that antibody against FMD virus serotypes O pan Asia, A Iran O5 and SAT2 / EGY/2012 by SNT and ELISA assay induced by the inactivated FMD adjuvant with rapeseed oil and ginseng vaccine and the inactivated FMD adjuvant with soybean oil and ginseng vaccine were higher than those induced by inactivated FMD adjuvant with Montanide oils 206. In contrast, the obtained antibody levels by the inactivated FMD adjuvant with rapeseed oil vaccine and inactivated FMD adjuvant with soybean oil vaccine was lower than those induced by inactivated FMD adjuvant with Montanide oils 206. So, it could be concluded that the use of plant origin oil adjuvant as rapeseed and soybean oil is considered a beneficial and alternative adjuvant to the imported mineral oil as it is much cheaper and induce better post vaccinal immune responses especially when mixed with ginseng extract.

Cinnamomum zeylanicum Essential Oil Reduces Infestation by Alphitobius diaperinus in Poultry Litter

Background: Even though insecticides are managed and the period of sanitary emptiness in poultry is respected, the elimination of Alphitobius diaperinus may not be successful. The use of essential oils of plant origin presents as a good alternative in the substitution of insecticides with synthetic molecules, since they are easy to obtain, with rapid degradation and without risk of residues for non-target organisms. The main objective of the present study was to examine whether Cinnamomum zeylanicum oil reduces Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) infestations under experimental conditions, without causing toxicity to broilers chicks exposed to treated litter.Materials, Methods & Results: The experimental design was completely randomized, with four replications per treatment. The treatments were as follows: solvent control using the diluent Dimethyl Sulfoxide 5% (oil diluent); chemical control using 5 g/m² cypermethrin; one spray of C. zeylanicum 5% oil; and two sprays of C. zeylanicum 5% oil. Each experimental unit was infested with 150 lesser mealworm adults. At 15 days of the broiler chick’s life, blood was collected for biochemi­cal analysis (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein, albumin, globulin, triglycerides and uric acid), and liver fragments were isolated for histopathological analysis. Using TupeTrap devices, we counted lesser mealworm 40 days after treatment. The treatments did not alter biochemical variables, and did not cause histopathological lesions in liver. The treatments with C. zeylanicum 5% oil with one and two sprays efficiently reduced lesser mealworm infestation compared with solvent control. Cypermethrin treatment had no effect.Discussion: Many of the commercial products present low effectiveness in the control of A. diaperinus, since the target organisms develop resistance to the product. In the present study, we used a higher cypermethrin dose than that recom­mended by the manufacturer, in order to increase efficacy in the face of possible resistance. Even so, cypermethrin did not efficiently control the organism. The effectiveness of the essential oil of C. zeylanicum tested can be attributed to the compounds found in greater quantity in the oil composition, such as cinnamaldehyde (41.27%), linalool (13.05%) and methyl eugenol (10.87%), characterized as responsible for the action of oil repellency. Monoterpenoid compounds found in essential oils extracted from plants have insecticidal action acting on the central nervous system of insects, which impairs their development, being characterized as neurotoxic compounds. The results found with the essential oil of C. zeylanicum are of great importance, since the control of A. diaperinus is not efficient because this organism has behavior that favors reinfestation in the poultry houses, such as shelter in cracks, in the draperies, below the feeders and in the soil. The biochemical analyzes of the blood can as important tools to assist in the monitoring of broilers health, in the diagnosis and treatment of diseases, and therefore the results presented are of great importance since they assist in the search for alternative methods for the control of A. diaperinus, where we can affirm that the essential oil of C. zeylanicum does not cause toxicity to broilers. Based on these results we can affirm that essential oil of Cinnamomum zeylanicum, 5%, is an effective substitute for existing commercially-available insecticides.Keywords: alternative control, cinnamon oil, insecticide, lesser mealworm.

Cinnamomum zeylanicum Essential Oil Reduces Infestation by Alphitobius diaperinus in Poultry Litter

Background: Even though insecticides are managed and the period of sanitary emptiness in poultry is respected, the elimination of Alphitobius diaperinus may not be successful. The use of essential oils of plant origin presents as a good alternative in the substitution of insecticides with synthetic molecules, since they are easy to obtain, with rapid degradationand without risk of residues for non-target organisms. The main objective of the present study was to examine whether Cinnamomum zeylanicum oil reduces Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) infestations under experimental conditions, without causing toxicity to broilers chicks exposed to treated litter.Materials, Methods & Results: The experimental design was completely randomized, with four replications per treatment. The treatments were as follows: solvent control using the diluent Dimethyl Sulfoxide 5% (oil diluent); chemical control using 5 g/m² cypermethrin; one spray of C. zeylanicum 5% oil; and two sprays of C. zeylanicum 5% oil. Each experimental unit was infested with 150 lesser mealworm adults. At 15 days of the broiler chick’s life, blood was collected for biochemical analysis (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein, albumin, globulin, triglycerides and uric acid), and liver fragments were isolated for histopathological analysis. Using TupeTrap devices, we counted lesser mealworm 40 days after treatment. The treatments did not alter biochemical variables, and did not causehistopathological lesions in liver. The treatments with C. zeylanicum 5% oil with one and two sprays efficiently reduced lesser mealworm infestation compared with solvent control. Cypermethrin treatment had no effect.Discussion: Many of the commercial products present low effectiveness in the control of A. diaperinus, since the target organisms develop resistance to the product. In the present study, we used a higher cypermethrin dose than that recommended by the manufacturer, in order to increase efficacy in the face of possible resistance. Even so, cypermethrin did not efficiently control the organism. The effectiveness of the essential oil of C. zeylanicum tested can be attributed to the compounds found in greater quantity in the oil composition, such as cinnamaldehyde (41.27%), linalool (13.05%) and methyl eugenol (10.87%), characterized as responsible for the action of oil repellency. Monoterpenoid compounds found in essential oils extracted from plants have insecticidal action acting on the central nervous system of insects, which impairs their development, being characterized as neurotoxic compounds. The results found with the essential oil of C. zeylanicum are of great importance, since the control of A. diaperinus is not efficient because this organism has behavior that favors reinfestation in the poultry houses, such as shelter in cracks, in the draperies, below the feeders and in the soil. The biochemical analyzes of the blood can as important tools to assist in the monitoring of broilers health, in the diagnosis and treatment of diseases, and therefore the results presented are of great importance since they assist in the search for alternative methods for the control of A. diaperinus, where we can affirm that the essential oil of C. zeylanicum does not cause toxicity to broilers. Based on these results we can affirm that essential oil of Cinnamomum zeylanicum, 5%, is an effective substitute for existing commercially-available insecticides.Keywords: alternative control, cinnamon oil, insecticide, lesser mealworm.

Potential health benefits of Sea buckthorn oil- A review

Hippophae rhamnoides, also known as sea buckthorn is an ancient plant with modern virtues, due to its nutritional and medicinal value. Sea buckthorn berry is rich in oil. Seeds (seed oil) and fruit pulp (pulp oil) are used for oil extraction. The oil content of Sea buckthorn pulp and seeds are in range of 18.2-43.5% and 7.03 -12.86% respectively. While linoleic (18:2n-6) and á-linolenic (18:3n-3) acids are the major fatty acids in the seed oil, the high level of palmitoleic acid (16:1n-7, up to 50%) differentiates sea buckthorn pulp oil from most other oils of plant origin. Oil from sea buckthorn contains several bioactive components such as vitamin E, vitamin K, carotenoids and phytosterols. Sea buckthorn oil has shown great healing effect in treating damaged mucous membranes of the gastrointestinal tract, wound, burn, dry eye, dermatitis and sunburn. The oil from pulp and the seeds of sea buckthorn have been shown to possess anti-diabetic, anti-atherogenic, hypocholesteromic, hypotensive, anti-hypoxic, antioxidant and anti-inflammatory properties. Sea buckthorn oils are used as a source for ingredients in several commercially available cosmetic products and nutritional supplements like jelly, plant capsules, or oral fluids.

Effect of the use of waste vegetable oil based biodiesel on the landscape in diesel engines

Petroleum-based fuels are now widely known as environmentally unfriendly because of non-renewable supplies and its contribution to environmental pollution. The challenge, therefore is to ensure appropriate energy supplies at minimum cost. There is an increasing energy demand in the world and nowadays it can be fulfilled only on the basis of fossil fuels. Therefore, it is necessary to evolve a renewable energy source with lower environmental impact. One alternative solution can be oils of plant origin, like vegetable oils and non-edible oils. With waste vegetable oil methyl ester, biofuel dependency can be decreased. Therefore, the aim of this research paper is to analyze the economic and environmental effect of waste vegetable oil methyl ester compared to fossil fuels. In some cases only the age of vehicles could raise burdens to biofuel utilization in road vehicles. Transport and energy policy ? on a large scale ? can play an important role in fuel consumption. Author is aware that waste vegetable oil methyl ester can play only a limited role in biofuel substitution.

Vegetable Oil: Nutritional and Industrial Perspective

Oils of plant origin have been predominantly used for food-based applications. Plant oils not only represent a non-polluting renewable resource but also provide a wide diversity in fatty acids (FAs) composition with diverse applications. Besides being edible, they are now increasingly being used in industrial applications such as paints, lubricants, soaps, biofuels etc. In addition, plants can be engineered to produce fatty acids which are nutritionally beneficial to human health. Thus these oils have potential to 1) substitute ever increasing demand of non –renewable petroleum sources for industrial application and 2) also spare the marine life by providing an alternative source to nutritionally and medically important long chain polyunsaturated fatty acids or ‘Fish oil’. The biochemical pathways producing storage oils in plants have been extensively characterized, but the factors regulating fatty acid synthesis and controlling total oil content in oilseed crops are still poorly understood. Thus understanding of plant lipid metabolism is fundamental to its manipulation and increased production. This review on oils discusses fatty acids of nutritional and industrial importance, and approaches for achieving future designer vegetable oil for both edible and non-edible uses. The review will discuss the success and bottlenecks in efficient production of novel FAs in non-native plants using genetic engineering as a tool.

Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections

To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L. The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candidaalbicans (12), C.dubliniensis (1), C.glabrata (3), C.krusei (3), C.parapsilosis (6), C.lusitaniae (1), C.famata (1) and C.tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs. Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC50=1.072mg/ml), but it can be exploited. V.agnus-castus seeds essential oils disosed a 35-58mm zone of inhibition (mean: 49mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13mg/ml V.agnus-castus seeds essential oils. Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.

Preparation of Highly Purified Stearidonic Acid from Echium Oil via an Enzymatic Method Combined with Preparative High Performance Liquid Chromatography.

Stearidonic acid (SDA), an n-3 polyunsaturated fatty acid (PUFA), can be obtained from plant origin oils and it can be a good source of PUFA for vegetarians. SDA can be easily converted to longer PUFA such as docosahexaenoic acid and eicosapentaenoic acid. Highly purified stearidonic acid (SDA) was prepared successfully from echium oil via an enzymatic method combined with preparative high performance liquid chromatography. In the 1(st) step, SDA enrichment was accomplished using Candida rugosa lipase and 39.5% of SDA was obtained in the fatty acid fraction. Subsequently, the 1(st) reaction mixture was used for the 2(nd) enzymatic esterification without any separation process. The 2(nd) esterification was conducted for further SDA enrichment in a packed-bed reactor using Lipozyme RM IM from Rhizomucor miehei and the SDA content increased in a very short residence time. Ethanol was selected as an appropriate alcohol to react as an acyl receptor, and the other conditions for SDA enrichment were optimized at 20°C of temperature, and 1:4 of molar ratio (i.e., fatty acid to ethanol). Under these conditions, 51.6% of SDA was obtained in the fatty acid fraction after a residence time of 15 min. Finally, highly purified SDA (purity, >99%) was obtained by prep-HPLC using the SDA-rich fraction obtained from the two-step lipase-catalyzed esterification.

Effect of monensin and vitamin E on milk production and composition of lactating dairy cows

Feeding unsaturated oils to lactating dairy cows impair ruminal biohydrogenation (BH) of unsaturated fatty acids (USFA) and increase ruminal outflow of BH intermediates such as trans-10, cis-12 CLA that are considered to be potent inhibitors of milk fat synthesis. Supplementing lactating dairy cow's rations containing plant origin oils with monensin and/or vitamin E may minimise the formation of trans-10 isomers in the rumen, thereby preventing milk fat depression. Therefore, this study was conducted to evaluate the effects of monensin and vitamin E supplementation in the diets of lactating dairy cows containing whole cottonseed, as the main source of FA on feed intake, milk production and composition, milk fatty acid profile, efficiency of nitrogen (N) utilisation, efficiency of net energy (NE) utilisation and nutrients digestibilities. Four multiparous Holstein lactating dairy cows (86±41 days in milk) were assigned to a balanced 4 × 4 Latin square design. Each experimental period lasted 21 days with a 14 days of treatment adaptation and a 7 days of data collection. The control diet was a total mixed ration (TMR) consisted of 430 g/kg forage and 570 g/kg of a concentrate mixture on dry matter (DM) basis. Cows were randomly assigned to one of the four dietary treatments including control diet (C), control diet supplemented with 150 mg of vitamin E/kg of DM (E), control diet supplemented with 24 mg of monensin/kg of DM (M) and control diet supplemented with 150 mg of vitamin E and 24 mg of monensin/kg of DM (EM). Dry matter intake (DMI) ranged from 19.1 to 19.5 kg/d and was similar among the dietary treatments. Dietary supplementation with vitamin E or monensin had no effect on milk production, milk fat, protein and lactose concentrations, efficiency of utilisation of nitrogen and net energy for lactation (NEL ). Digestibility of DM, organic matter (OM), crude protein (CP) and ether extract (EE) was not affected by the dietary treatments. Digestibility of neutral detergent fibre (NDF) was higher in cows fed with the M and EM diets in relation to those fed the C and E diets. The concentrations of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C15:0, trans-10-16:1, cis-9-16:1, 17:0, 18:0, trans-11-18:1, cis-9-18:1, cis-9, trans-11 conjugated linoleic acid (CLA), trans-10, cis-12 CLA, and 18:3n-3 FA in milk fat were not affected by the dietary supplementations. While feeding the M diet tended to decrease milk fat concentration of C16:0, the milk fat concentration of C18:2n-6 FA tended to be increased. Dietary supplementation with vitamin E or monensin had no effect on milk fat concentrations of saturated, unsaturated, monounsaturated, polyunsaturated, short chain and long chain FA, but feeding the M diet numerically decreased milk fat concentration of medium chain fatty acids (MCFA). The results showed that vitamin E and/or monensin supplementations did not improve milk fat content and did not minimise the formation of trans-10 FA isomers in the rumen when whole cottonseed was included in the diet as the main source of fatty acids.

Antifungal properties of Artemisia sieberi and Origanum vulgare essential oils against Candida glabrata isolates obtained from patients with vulvovaginal candidiasis

To study the composition and anti-Candida glabrata activity of Artemisia sieberi and Origanum vulgare essential oils. The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). Susceptibility tests were expressed as inhibition zone by the agar-well diffusion method and minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method. Transmission electron microscopy (TEM) was performed to reveal the effects of the essential oils on the morphology of C. glabrata. Major components were β-thujone (23%), camphor (19.5%) and α-thujone (15%) in A. sieberi essential oil and linalool (42%), thymol (25.1%) and α-terpineol (10%) in O. vulgare essential oil. A. sieberi caused an 11–40 mm zone of inhibition (mean: 23.4 mm) while O. vulgare showed an 18–40 mm zone of inhibition (mean: 27.1 mm) against all 16 isolates tested. In broth macrodilution method, all the tested C. glabrata isolates were sensitive to the essential oils and this activity was concentration-dependent. MIC values varied from 37.4 to 4781.3 μg/ml with A. sieberi (mean: 1496.4 μg/ml) and 0.5 to 1100 μg/ml with O. vulgare (mean: 340.2 μg/ml) essential oils. Results of this study indicated that the oils of plant origin could be used as potential anti-C. glabrata agents. Étudier la composition et l’activité antifongique des huiles essentielles d’Artemisia sieberi et d’Origanum vulgare sur Candida glabrata. Les huiles essentielles ont été extraites en utilisant l’appareil de Clevenger-Type et analysées par la chromatographie gazeuse couplée à la spectrométrie de masse (CG/SM). Les tests de sensibilité ont été évalués par la méthode de diffusion en gélose, par la concentration minimale inhibitrice (CMI) et par la concentration minimale fongicide (CMF) selon la méthode de macrodilution en milieu liquide. La microscopie électronique à transmission (TEM) a été réalisée pour révéler les effets de l’huile essentielle sur la morphologie de C. glabrata. Les composants majeurs étaient β-thujone (23 %), camphre (19,5 %) et α-thujone (15 %) dans l’huile essentielle d’A. sieberi et linalool (42 %), thymol (25,1 %) et α-terpineol (10 %) dans l’huile essentielle d’O. vulgare. A. sieberi a montré une zone d’inhibition de 11–40 mm (moyenne : 23,4 mm) alors qu’O. vulgare a montré une zone d’inhibition de 18–40 mm (moyenne : 27,1 mm) contre les 16 isolats testés. Dans la méthode de macrodilution en milieu liquide, tous les isolats de C. glabrata testés étaient sensibles aux huiles essentielles et cette activité était dépendante de la concentration. Les valeurs de CMI variaient de 37,4 à 4781,3 μg/ml pour A. sieberi (moyenne : 1496,4 μg/ml) et de 0,5 à 1100 μg/ml pour O. vulgare (moyenne : 340,2 μg/ml). Les résultats de cette étude ont montré que les huiles essentielles de plantes pourraient être utilisées comme des agents antifongiques potentiels contre C. glabrata.

LOW TEMPERATURE PLANAR CHROMATOGRAPHY–DENSITOMETRY AND GAS CHROMATOGRAPHY OF ESSENTIAL OILS FROM DIFFERENT SAGE (SALVIA) SPECIES

Essential oils of plant origin are the multicomponent mixtures of mono-, di-, tri-, and sesquiterpenes. Due to their recognized curative, cosmetic, and nutritional properties on the one hand and an outstanding modern analytical potential on the other, qualitative and quantitative composition of essential oils currently is in the focus of interest for phytochemistry and pharmacognosy. Due to the recognized volatility of the essential oil components, in their case the analytical method of choice is gas chromatography with mass spectrometric detection (GC-MS). However, great versatility of planar chromatography has resulted in a number of successful applications of this relatively simple and inexpensive separation technique to the investigations on composition of the volatile plant constituents as well. Generally, the low temperature preparative layer chromatography (PLC) is used for preliminary fractionation of the essential oils, and the separated fractions are further analyzed by means of GC-MS. In this study, we scrutinized a possibility of using the low temperature analytical thin-layer chromatography (TLC) to fingerprinting of the essential oils originating from the five different sage (Salvia) species, i.e., S. lavandulifolia, S. staminea, S. hians, S. triloba, and S. nemorosa. We also used the low temperature PLC for the preliminary fractionation of these essential oils prior to the GC-MS analysis. It was shown that the low temperature TLC can successfully be applied to fingerprinting the different sage (Salvia) species. Fractionation of the essential oils from the sage species by means of the low temperature PLC prior to the GC-MS analysis is also possible, although individual stages of the approach still need an additional optimization.

A Novel method for spectrophotometric determination of triglycerides

AbstractA new method for the spectrophotometric determination of triglycerides at microgram levels has been developed. The method relies on the quantitative degradation ofO‐acyl lipids with hydrazine and the subsequent conversion of the fatty acid hydrazides to the correspondingN′‐isopropylidenealkanohydrazides by reaction with acetone. Results are presented for triolein and some oils of plant origin.