Imbibition oil recovery is an effective technology for tight reservoir development, the working mode of the well needs to be switched between production and injection, when using imbibition oil recovery. It will leads to some disadvantage of imbibition oil recovery, such as long operation time and high development cost. Therefore, a novel integrated technology of separate layer production and injection has been proposed, which is based on the hydraulic feedback pump and bridge concentric zonal water injection technology. When switching the working mode of the well, it is only necessary to trip in or out the plunger into the cylinder of pump. Until recently, this technology had been applied in 3 wells for field test, 2 injection wells and 1 production well. The test results show that this technology can realize switching the working mode of the well without replacing the pipe string. Besides, it also can meet the requirements of tripping in/out the plunger ϕ50.8mm) or the measuring instrument ϕ42mm). The successful field test of the separate layer production and injection integrated pipe string is very important for changing development method of low-permeability reservoirs from conventional waterflooding to imbibition oil recovery. By using this integrated technology, low permeability reservoirs can be developed economically and effectively.