Officinale Rhizome Research Articles

Species delimitation in Pythium species complexes: the case of Pythium myriotylum Drechsler and Pythium zingiberis Takahashi

Pythium myriotylum and P. zingiberis have both been implicated in soft rot of ginger (Zingiber spp.). The status of these two taxa as distinct species follows original descriptions of physiology, morphology and pathogenicity. However, their status has been questioned by phylogenetic analyses. In this study, putative P. zingiberis isolates recovered from edible ginger (Z. officinale) rhizomes with Pythium soft rot (PSR) disease sampled from Queensland, Australia, were compared with reference isolates of P. myriotylum from peanut in Israel (CBS254.70) and P. zingiberis from ginger in Japan (NBRC30817). All isolates differed slightly in temperature optima for growth and produced similar sizes of oogonia and oospores. The sequence homology of 20 gene fragments retrieved from nuclear and mitochondrial genomes ranges from over 99 to 100% to each other. In vitro pathogenicity assays were conducted on excised carrot, ginger, potato, radish and sweet potato tuber/root sections and on seeds and seedlings of cucumber, cauliflower, millet, rye, sweet corn, tomato and wheat with each of the isolates. The reference isolate P. zingiberis NBRC30817, which was previously believed to have a narrow host range, was pathogenic on a number of tested plant species. Analysis of this comprehensive set of data allowed us to assign all tested isolates, including the isolate P. zingiberis NBRC30817, to the taxon P. myriotylum, thus confirming that the causal pathogen of PSR disease of ginger in Australia is P. myriotylum.

Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3

ABSTRACTFungal endophytes are less studied group of microorganisms with vast therapeutic potential. A polyphenol producing endophyticfungus was isolated from Zingiber officinale rhizome. The ethyl acetate extract of Aspergillus austroafricanus (EAE) was tested against five human pathogenic bacteria by disc diffusion method. Antioxidant activity of EAE was determined by DPPH, H2O2 and nitric oxide radical-scavenging methods. DNA protection from the OH radicals was tested by agarose gel electrophoresis. High-performance liquid chromatography was used to determine the total yield of polyphenols. The identity of the endophytic fungus was established as A. austroafricanus CGJ-B3 (GenBank accession No. KT780617) based on rDNA and phylogenetic analysis. EAE showed significant antioxidant, antimicrobial activity and DNA damage protection capacity. The HPLC analysis showed the presence of polyphenols such as p-coumaric acid, ferulic acid and cinnamic acid and the content was about 0.392 ± 0.08 µg/mg, 4.35 ± 0.16 µg/mg and 1.976 ± 0.11 µg/mg, respectively. A. austroafricanus CGJ-B3 isolated from Z. officinale is a promising potential pharmaceutical agent and can be used as an alternative source of polyphenols like p-coumaric acid, ferulic acid and cinnamic acid.

Antimicrobial Activity of Essential Oil of <i>Zingiber officinale</i> Roscoe (Zingiberaceae)

Zingiber officinale, commonly known as ginger, is a species native of tropical Asia. It is grown and used in tropical countries of America, including Mexico, where it is used for the treatment of throat infections. The aim of this study was to determine the chemical composition of essential oil of Z. officinale rhizome and evaluate their antimicrobial activity. The essential oil was obtained by hydrodistillation, and the chemical composition of the oil was determined by GC-MS. To determine the antimicrobial activity diffusion, agar dilution and radial growth inhibition methods were employed. Microbial growth curves were performed on the strains most susceptible. It was identified 28 compounds in the essential oil, the most abundant were eudesmol (8.19%), γ-terpinene (7.88 %), a-curcumene (7.28%), alloaromadendrene (6.56%), zingiberene (6.06 %), α-pinene (5.76 %), δ-cadinene (3.84%), elemol (3.39%), farnesal (3.45%), E-β-farnesene (3.57%), neril acetate (2.8%) and β-myrcene (2.94%). In antimicrobial activity, the essential oil inhibited the growth of 15 bacterial strains, 3 of yeast fungi and 4 of mycelia fungi. The most susceptible strains were S. aureus FES-I (MIC = 0.25 mg/mL) S. epidemidis FES-C (MIC = 0.5 mg/mL), E. faecalis ATCC 14506 (MIC = 1.0 mg/mL), C. tropicalis (CMI = 0.125 mg/mL) and T. mentagrophytes (CF50 = 0.08 mg/mL). In the microbial growth curves the essential oil showed bactericidal effect on S. aureus FES-I and S. marcescens ATCC 14756 from the first hour of exposure of the strains to oil, eliminating 99.9% of CFU in concentrations of 0.5 and 0.75 mg/mL respectively. The results validate the medicinal use of Z. officinale in the treatment of diseases of possible infectious origin.

Laurus nobilis, Zingiber officinale and Anethum graveolens Essential Oils: Composition, Antioxidant and Antibacterial Activities against Bacteria Isolated from Fish and Shellfish.

Several bacterial strains were isolated from wild and reared fish and shellfish. The identification of these strains showed the dominance of the Aeromonas hydrophila species in all seafood samples, followed by Staphylococcus spp., Vibrio alginolyticus, Enterobacter cloacae, Klebsiella ornithinolytica, Klebsiella oxytoca and Serratia odorifera. The isolates were studied for their ability to produce exoenzymes and biofilms. The chemical composition of the essential oils from Laurus nobilis leaves, Zingiber officinale rhizomes and Anethum graveolens aerial parts was studied by GC and GC/MS. The essential oils’ antioxidant and antibacterial activities against the isolated microorganisms were studied. Low concentrations of the three essential oils were needed to inhibit the growth of the selected bacteria and the lowest MBCs values were obtained for the laurel essential oil. The selected essential oils can be used as a good natural preservative in fish food due to their antioxidant and antibacterial activities.

COMPARATIVE PHARMACOGNOSTICAL EVALUATION OF COSTUS SPECIOSUS (WILD GINGER) AND ZINGIBER OFFICINALE (GINGER) RHIZOME

Objective: Costus speciosus Koen. (Ginger) is an Indian ornamental plant, belonging to family Costaceae. The present research work deals with the establishment of standardization parameter regarding comparative pharmacognostic study between Costus speciosus and Zingiber officinale rhizome.Methods: Macroscopic and microscopic evaluation, physicochemical parameters like ash values, moisture content, extractive value, fluorescence analysis and the preliminary phytochemical investigation was done.Results: The Macroscopic difference was found as an absence of odor and taste in Costus speciosus and presence of pungent and aromatic odor in Zingiber officinale. Oil globule, tracheids, starch granules in Zingiber officinale was found whereas the absence of the same in Costus speciosus rhizome was found in the microscopic evaluation. Fluorescence study also exhibits differences. The presence of saponin, carbohydrate, glycosides in Costus speciosus whereas Zingiber officinale revealed the presence of alkaloids, glycosides, flavonoids and protein.Conclusion: These finding can serve as the basis for Identification and investigation of Costus speciosus and Zingiber officinale rhizome and can also establish the characteristic differentiating features of both.

EVALUATION OF ANTIFUNGAL ACTIVITY OF A 3-EXTRACT FORMULATION IN TOPICAL DOSAGE FORM AND ITS SAFETY ASPECT

<p>ABSTRACT<br />Objective: To determine the combination of extract of Rosmarinus officinalis leaves, Zingiber officinale rhizome, and Curcuma xanthorrhiza rhizome in<br />2 form topical preparations, cream, and ointment, through in situ study, as well as to evaluate safety aspect of both cream and ointment.<br />Methods: Irritation skin and eye test were performed according to OECD guideline. Rabbits were also used as an object of the in situ study. A suspension<br />of Trichophyton mentagrophytes was injected through the skin on both sides of rabbit’s back to induce dermatophytosis. Throughout the treatment<br />period, topical preparation was applied to the infected site until the wound was fully recovered.<br />Results: There was no significant irritation effect on both topical dosage forms. However, an incidence of redness was observed in the skin applied<br />by cream. Healing rate of extract in ointment.<br />Conclusion: Combination of the corresponding extract showed antifungal activity and was comparable to that of ketoconazole as a reference drug.<br />Keywords: Rosmarinus officinalis leaves, Zingiber officinale rhizome, Curcuma xanthorrhiza rhizome, In situ, Irritation.</p>

Ovicidal effects of indigenous plant extracts alone and with triazophos 40 EC on Spodoptera litura (F.)

Against Spodoptera litura (F.) the plant extracts when evaluated neem seed extract 5% recorded minimum egg hatching followed by subza (Ocimum basilicum) leaf extract and these were significantly superior over marigold (Tagetes erecta) leaf extract, orange (Citrus reticulata) peel extract and ginger (Zingiber officinale) rhizome extract @ 5% each. However, none of these alone could surpass the effects of trizophos 40 EC. There was an increase in the ovicidal activity when these extracts were mixed with trizophos 40 EC compared with their individual effects. All the plant extracts and their combinations with trizophos 40 EC were effective in reduced egg hatching but not effective compared to trizophos. The post hatching observations indicated that the moribund larvae were more with leaf extracts.

Evaluation of the Antifungal Activity of Aqueous and Alcoholic Extracts of Six Spices

Background: Nigeria is plagued with a variety of socioeconomic problems mainly poverty, poor access to quality healthcare and poor hygienic conditions resulting in the myriad of fungal infections that are frequently encountered in clinical practice. Method: The antifungal activity of aqueous, methanolic, propanolic and benzyl alcohol extracts of Capsicum annuum fruits and seeds, Capsicum chinense fruits and seeds, Aframomum melegueta pods and seeds, Allium sativum bulbs, Allium cepa bulbs and Zingiber officinale rhizomes on Candida albicans (yeast), Aspergillus niger (mould) and Trichophyton rubrum (dermatophyte) were evaluated by the agar well diffusion method. The aqueous and alcoholic filtered plant extracts were obtained by maceration, and also methanolic extracts were also obtained by Soxhlet extraction. The results were obtained by measuring the inhibition zone diameter in millimeters and were presented by subtracting the activity of the control. Results: Aqueous and methanolic extracts of Allium sativum gave the highest inhibition of the growth of Candida albicans (22 mm), followed closely by its propanolic extract with inhibition zone diameter of 15 mm, and also, propanolic extracts of Aframomum melegueta and Allium cepa gave inhibition zone diameters of 12 mm each. Soxhlet methanolic extract of Allium sativum had the highest inhibition of the growth of Aspergillus niger with an inhibition zone diameter of 25 mm, followed closely by Zingiber officinale Soxhlet methanolic extract with an inhibition zone diameter of 22 mm, also, the propanolic extract of Allium sativum gave an inhibition zone diameter of 21 mm, whereas Soxhlet methanolic extracts of Aframomum melegueta and Allium cepa gave an inhibition zone diameter of 19 mm each. The highest activity against Trichophyton rubrum was obtained with the Soxhlet methanolic extract of Allium sativum (39 mm), followed closely by its propanolic extract with an inhibition zone diameter of 27 mm. An inhibition zone diameter of 22 mm was recorded with the benzyl alcohol extract of Allium cepa, 22 mm with the Soxhlet methanolic extract of Aframomum melegueta and 19mm with the aqueous extract of Capsicum chinense seeds. Conclusion: The in-vitro inhibitory effects of these spice extracts indicated that the test spices could serve as potential candidates for developing new systemic and topical antifungal drugs against the wide range of pathogenic fungal strains, and they could also serve as natural prophylaxis against the fungal infections.

Structural Elements and Cough Suppressing Activity of Polysaccharides from Zingiber officinale Rhizome.

Zingiber officinale is used for the management of fever, bronchial asthma and cough for thousands of years. While the link to a particular indication has been established in human, the active principle of the formulation remains unknown. Herein, we have investigated a water extracted polysaccharides (WEP) containing fraction from its rhizome. Utilizing a traditional aqueous extraction protocol and using chemical, chromatographic and spectroscopic methods a fraction containing a branched glucan and polygalaturonan in a ratio of 59:1 was characterized. This glucan, which has a molecular mass of 36 kDa, is made up of terminal-, (1,4)- and (1,4,6)-linked α-Glcp residues. Oral administration of WEP in doses of 25 and 50 mg/kg body weight significantly inhibited the number of citric acid-induced cough efforts in guinea pigs. It does not alter the specific airway smooth muscle reactivity significantly. Thus, traditional aqueous extraction method provides molecular entities, which induces antitussive activity without addiction.

English

The present study was conducted to investigate antioxidant and antimicrobial activities of Zingiber officinale, and detected possible chemicals existence in the plant. Phytochemical screening was carried out using the standard test methods of different chemical groups. Investigating the antioxidant activity, one complementary test method namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was carried out. Disc diffusion method, with minor modifications was used for the evaluation of in vitro antimicrobial activity. The extracts were a rich source of phytochemicals. In DPPH free radical scavenging test, the ethanol, acetone and cyclohexane extracts show the highest free radical scavenging activities, although the extracts revealed good antimicrobial activities. Finally, the bioactive compounds of Zingiber officinale rhizomes have been evaluated by using GC-spectroscopy. Key words: Zingiber officinale, GC spectroscopy antimicrobial, antioxidant, agar-well diffusion, phytochemical screening.

Biogenic Synthesis of Silver Nanoparticles Using Cnidium officinale Extract and Their Catalytic Reduction of 4-Nitroaniline

Silver nanoparticles (AgNPs) with two different morphologies were synthesized using aqueous extract of Cnidium officinale rhizomes under different reaction conditions and applied to the facile aqueous phase reduction of 4-nitroaniline (4-NA) as catalysts. The synthesized AgNPs are characterized by UV–Visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) method and high resolution transmission electron microscopy (HR-TEM) with energy dispersive X-ray spectroscopy (EDS) analysis. HR-TEM showed that the synthesized AgNPs had two different morphological forms; truncated triangular prism shapes with a mean size of ~35 nm and spherical shapes with a mean size of ~9 nm. The HR-TEM and XRD results are consistent with the standard values. The catalytic activities of both AgNPs were compared on the reduction of 4-NA in water by NaBH4 as a reducing agent. The reduction of 4-NA was monitored using UV–Visible spectroscopy. The ~9 nm (spherical) sized AgNPs showed superior catalytic activity than the larger AgNPs. The calculated rate constant (k) for the catalytic reduction of 4-nitroanilie by spherical AgNPs was 11.16 × 10−3 s−1.

Zingiber officinale Roscoe aqueous extract modulates Matrixmetalloproteinases and tissue inhibitors of Metalloproteinases expressions in Dengue virus-infected cells: implications for prevention of vascular permeability

Purpose: To investigate the effect of the aqueous extract of Zingiber officinale Roscoe. (ZOA) rhizome on the activity and expression of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 using an in vitro model of Dengue virus (DV) infection. Methods: Z. officinale rhizomes were extracted with water by continuous shaking for 5 days. The total phenolic content in extract was measured by Folin-Ciocalteu method. High performance liquid chromatography (HPLC) was employed to define qualitative and quantitative content of [6]-gingerol in ZOA. The median inhibitory concentration (IC50) value of ZOA for Vero cells was determined by 3-(4,5 dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide (MTT) assay. To induce MMPs production, Vero cells were infected with DV3. The modulatory effect of ZOA on the activity and expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 were assessed using gelatin zymography and quantitative Real-time polymerase chain reaction (RTPCR), respectively. Results: The yield of the ZOA was 7.98%. Total phenolics in ZOA was 68.17 ± 0.28 mg GAE/g of extract and it contained 29.32 ± 1.97 mg 6-gingerol/g of extract.The half-maximal inhibition concentration (IC50) of ZOA was 348.8 μg/mL for Vero cells. DV infection of Vero cells significantly elevated the production of soluble gelatinolytic MMP-2 and to a lesser extent, MMP-9, and their activities were significantly inhibited by ZOA in a dose-dependent manner. A significant down-regulation of MMP-2, MMP-9 mRNA expression and up-regulation of TIMP-1, TIMP-2 mRNA expression were observed in DV-infected Vero cells following treatment with ZOA, and it occurred in a dose-dependent manner. Conclusion: The findings of this study suggest that ZOA may ameliorate plasma leakage in dengue virus infection and decrease the chances of severe dengue complications, dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS) by inhibiting the activities and expression of MMP-2 and MMP-9 while upregulating the expression of TIMP-1 and TIMP-2.

Amelioration of pancreatic and renal derangements in streptozotocin-induced diabetic rats by polyphenol extracts of Ginger (Zingiber officinale) rhizome

Free and bound polyphenol extracts of Zingiber officinale rhizome were investigated for their antidiabetic potential in the pancreatic and renal tissues of diabetic rats at a dose of 500mg/kg body weight. Forty Wistar rats were completely randomized into five groups: A–E consisting of eight animals each. Group A (control) comprises normal healthy animals and were orally administered 1.0mL distilled water on a daily basis for 42 days while group B–E were made up of 50mg/kg streptozotocin (STZ)-induced diabetic rats. Group C and D received 1.0mL 500mg/kg body weight free and bound polyphenol extracts respectively while group E received 1.0mL 0.6mg/kg of glibenclamide. Administration of the extracts to the diabetic rats significantly reduced (p<0.05) serum glucose and urea concentrations, increased (p<0.05) serum insulin and Homeostatic Model Assessment for β-cell dysfunction (HOMA-β) while the level of creatinine and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) were not affected. Histological examination of the pancreas and kidney revealed restoration of the structural derangements caused by streptozotocin in the polyphenol extracts treated diabetic rats compared to the control groups. Therefore, polyphenols from Zingiber officinale could ameliorate diabetes-induced pancreatic and renal derangements in rats.

English

Rotten white yam (Dioscorea rotundata) tuber samples were collected from farms in three communities. Nine fungal organisms were isolated by direct tissue plating on potato dextrose agar medium. These were: Fusarium oxysporum, Aspergillus flavus, Aspergillus niger, Penicillium spp., Lasiodiplodia theobromae, Trichoderma viride, Rhizopus spp., Pestalotia guepini and Alternaria solani. Each of these isolates caused rot lesions when inoculated into healthy yam tubers. These organisms were re-isolated, identified and compared with the initial isolates to confirm their pathogenicity. L. theobromae, A. niger, Rhizopus sp. and A.&nbsp; solani were the most frequently isolated fungi species from the rotten yam tuber samples, with frequency of isolation of 30.07, 16.08, 16.08 and 12.59%, respectively. Zingiber officinale rhizome and Piper nigrum seeds were tested against L. theobromae and F. oxysporum in vitro. The botanical extracts were prepared by cold water extraction method at a concentration of 60% w/v. The two extracts showed significance in inhibiting the growth of the two fungi when data was statistically analysed (p = 0.05) using Genstat 9.2 package. This implies that they have some anti-fungal properties which need to be further investigated in vivo to establish their suitability in protecting yam tubers from storage rot. However, P. nigrum showed to be much stronger than ginger in terms of bioactivity. Key words: Zingiber officinale, Piper nigrum, bioactivity, yam tuber rot, rot fungi.

Modulatory activities of Zingiber officinale Roscoe methanol extract on the expression and activity of MMPs and TIMPs on dengue virus infected cells

ObjectiveTo evaluate the effect of methanolic extract of Zingiber officinale (ZOM) rhizome on the activity and expression profile of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2 at the mRNA level in dengue virus infected Vero cells. MethodsTotal phenolic content and [6]-gingerol content in ZOM were determined by utilizing Folin-Ciocalteu reagent and high performance liquid chromatography. IC50 value of ZOM for Vero cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Vero cells were infected with dengue virus to induce MMPs production. Modulatory effect of ZOM on the activity and expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 were demonstrated by using gelatin zymography and real time RT-PCR respectively. ResultsAmount of total phenolics in ZOM in terms of mg gallic acid equivalents/g was (252.89 ± 0.56) and it possessed (137.32 ± 2.47) mg [6]-gingerol content per gram of extract. The IC50 value of ZOM was 221.5 μg/mL for Vero cells. The activities of MMP-2 and to a lesser extent MMP-9 were significantly enhanced in the conditioned media collected from the dengue virus infected Vero cells compared to conditioned media from non-infected cells and their activities were significantly inhibited by ZOM in dose-dependent manner. ZOM significantly downregulated the mRNA expression of MMP-2 and MMP-9 and upregulated the mRNA expression of TIMP-1 and TIMP-2 in dengue virus infected Vero cells in concentration-dependent manner. ConclusionsThe results of this study suggest that ZOM may be effective in the control of dengue-virus-induced permeability through the reduction of activities and expression of proteases which degrade the adhesion molecules between cells. This may provide the basis for developing new and effective methods in controlling severe dengue complications.

Effect of Extraction Method on Total Flavonoid, Total Phenolic Content, Antioxidant and Anti-bacterial Activity of Zingiberis Officinale Rhizome

This research aims to compare between decocta and maceration method using water and ethanol as a solvent on total flavonoid, total phenolic content, antioxidant and antibacterial activity of Zingiber officinale rhyzome extract. The Rhyzome was dryed by oven method on 50°C for 24 hours and 48 hours. Total flavonoid, total phenolic content and antioxidant activity were assay by spectrophotometric method. The antibacterial activity was determined by difussion agar method against Bacillus subtilis (Bs) and Escherichia colli InaCC B5 (Ec). Ethanol extract from dried rhyzome processed for 24 hours (R-24) indicated the best result for total flavonoid, total phenolic content and antioxidant activity. The yield of total flavonoid for R-24 was 286 μg/mL. The yield of total phenolic content for R-24 was 504 μg/mL and the yield of IC50 antioxidant activity for R-24 was 5.753 μg/mL. The extract should antibacterial activity againts Bs with inhibition diameter of 10% and 20% of samples concentration are 10 and 11.5 mm respectively. Meanwhile, The inhibition diameter result against Ec for 10% and 20% of samples concentration are 7.5 and 8.5 mm respectively.

Three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes

The present work describes for the first time an elegant non-chromatographic method, the three phase partitioning for the purification and recovery of zingibain, a milk-clotting enzyme, from Zingiber officinale rhizomes. Factors affecting partitioning efficiency such as (NH4)2SO4 saturation, crude extract to t-butanol ratio and pH on zingibain partitioning were investigated. Optimal purification parameters were 50% (NH4)2SO4 saturation with 1.0:1.0 ratio of crude extract:t-butanol at pH 7.0, which gave 14.91 purification fold with 215% recovery of zingibain. The enzyme was found to be exclusively partitioned in the aqueous phase. The enzyme showed a prominent single band on SDS-PAGE. It is a monomeric protein of 33.8kDa and its isoelectric point is 4.38. The enzyme exhibited maximal proteolytic activity at a temperature of 60°C and pH 7.0. It was found to be stable at 40–65°C during 2h. The enzyme was found to be highly stable against numerous metal ions and its activity was enhanced by Ca2+, K+ and Na+. It was completely inhibited by heavy metal ions such as Cu2+ and Hg2+ and partially by Cd+. Zingibain milk-clotting activity (MCA) was found to be highly stable when stored under freezing (−20°C) for 30 days compared at 4°C.

BIOEFFICACY OF ZINGIBER OFFICINALE AGAINST PROSTEPHANUS TRUNCATUS HORN (COLEOPTERA: BOSTRICHIDAE) INFESTING MAIZE.

ABSTRACT Maize is an important cereal crop grown in sub-Saharan Africa however, greater part of the grains produced is destroyed in storage by Prostephanus truncatus Horn (Coleoptera: Bostrichidae). In this study, the bio-activities of Zingiber officinale rhizome (Ginger), was investigated in the laboratory against P. truncatus at different proportions (25%, 20%, 15%, 10% and 5% wt/wt) with Actellic dust insecticide as a reference insecticide. The highest proportions (25% wt/wt) of Z. officinale powder significantly reduced the survival of P. truncatus to 0% after 12 days of treatment and the LD50 of the dust was obtained as 6.72%. The oil extract of Z. officinale at different concentrations (700 µL/mL, 350 µL/mL, 175 µL/mL, 87.5 µL/mL and 44 µL/mL) and Agricombi insecticide as the reference was equally evaluated for the following: contact toxicity on adult insect by dipping, grain residual treatment, repellency effect, effect on progeny and adult emergence in treated grains and damage assessment to grains. There was no survival (0%) recorded at 700 µL/mL both in contact and residual treatment of P. truncatus. The LD50 of the oil extract was obtained as 84.78 µL/mL. Grains treated with the different concentrations of Z. officinale oil significantly repelled insects with the highest concentration of 700 µL/mL yielding 100% repellency. The least mean adult emergence of 0.0 was recorded for both concentrations of 700 µL/mL and 350 µL/mL in treated grains. Grain damage by P. truncatus was significantly reduced when treated with Powder and oil extract of Z. officinale compared to the untreated grains.

The effect of hydroalcoholic extract of Zingiber officinale rhizomes on mechanical activity of isolated trachea of male rats

The effect of hydroalcoholic extract of Zingiber officinale rhizomes on mechanical activity of isolated trachea of male rats

Atividade antimicrobiana do óleo essencial de rizomas de açafrão (Curcuma longa L.) e gengibre (Zingiber officinale Roscoe) frente a salmonelas entéricas isoladas de frango resfriado

Objetivou-se com este trabalho identificar, quantificar os constituintes, e avaliar a atividade antibacteriana dos óleos essenciais extraídos de rizomas de açafrão (Curcuma longa L.) e gengibre (Zingiber officinale Roscoe) cultivados nas condições de Manaus/AM frente a 14 salmonelas entéricas isoladas de frango resfriado. A extração dos óleos essenciais foi realizada utilizando-se aparelho tipo Clevenger e a composição determinada por Cromatografia Gasosa acoplada a Espectrometria de Massas (CG-MS). A atividade antibacteriana foi realizada com o emprego de técnica de microdiluição em caldo. O óleo essencial de gengibre se mostrou expressivamente mais eficiente do que o óleo de açafrão, tanto em termos de ação bacteriostática (concentração inibitória mínima de 2500 a 5000 µg.mL-1) quanto bactericida (concentração bactericida mínima de 5000 a 10000 µg.mL-1) observando-se variação apenas em duas as amostras em termos de resistência a ação bactericida deste óleo. Assim, o óleo essencial de gengibre, representa uma alternativa para o controle de Salmonella enterica, entretanto, demais estudos abordando o sinergismo com alimentos são indicados.