Abstract
ABSTRACTFungal endophytes are less studied group of microorganisms with vast therapeutic potential. A polyphenol producing endophyticfungus was isolated from Zingiber officinale rhizome. The ethyl acetate extract of Aspergillus austroafricanus (EAE) was tested against five human pathogenic bacteria by disc diffusion method. Antioxidant activity of EAE was determined by DPPH, H2O2 and nitric oxide radical-scavenging methods. DNA protection from the OH radicals was tested by agarose gel electrophoresis. High-performance liquid chromatography was used to determine the total yield of polyphenols. The identity of the endophytic fungus was established as A. austroafricanus CGJ-B3 (GenBank accession No. KT780617) based on rDNA and phylogenetic analysis. EAE showed significant antioxidant, antimicrobial activity and DNA damage protection capacity. The HPLC analysis showed the presence of polyphenols such as p-coumaric acid, ferulic acid and cinnamic acid and the content was about 0.392 ± 0.08 µg/mg, 4.35 ± 0.16 µg/mg and 1.976 ± 0.11 µg/mg, respectively. A. austroafricanus CGJ-B3 isolated from Z. officinale is a promising potential pharmaceutical agent and can be used as an alternative source of polyphenols like p-coumaric acid, ferulic acid and cinnamic acid.
Highlights
Plants are the reservoir of therapeutic compounds like phenolics, flavonoids, terpenoids and alkaloids
A polyphenol and flavonoid producing fungus was isolated from the rhizome of Z. officinale
We have successfully isolated an endophytic fungus A. austroafricanus CGJ-B3 from the inner tissues of Z. officinale rhizome
Summary
Plants are the reservoir of therapeutic compounds like phenolics, flavonoids, terpenoids and alkaloids. Zingiber officinale is one of the important tropical medicinal plants which belongs to the family Zingiberaceae This plant was used as an ingredient in Ayurveda and Siddha medicine since ages for treating various ailments (Kumar et al 2011). Different parts of Z. officinale have been reported to show an array of medicinal properties such as analgesic, anti-inflammatory, antiulcer, antioxidant (Ma et al 2004; Chrubasik et al 2005; Ghasemzadeh et al 2010; Nanjundaiah et al 2011a), anticancer (Miyoshi et al 2003; Shukla & Singh 2007), antimicrobial (Aghazadeh et al 2016), anticoagulant (Srivas 1984), fibrinolytic, prebiotic (Helal et al 2014), immunomodulatory (Gupta & Chaphalkar 2015), hepatoprotective, larvicidal (Kumar et al 2011) and antiLimnatisnilotic activities (Bahmani et al 2013). It is a potent inhibitor of proton–potassium ATPase activity as well as the ulcer-causing organism, Helicobacter pylori (Siddaraju & Dharmesh 2007)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
