OD Groups Research Articles

Risk, reversibility and resilience of brain circuitries linked to opioid dependence: A diffusion tensor imaging study of actively opioid-using subjects and three comparison groups.

Drug addiction is linked with micro-structural changes in the fronto-striatal white matter (WM) tracts. However, little is known regarding causality (risk factor for subsequent drug use vs. effect of chronic drug use) and reversibility of such changes. In this study, 30-direction diffusion tensor imaging (DTI) was used to examine WM integrity of selected fronto-striatal circuitries: the orbito-frontal circuit (OFC), anterior cingulate (AC), inferior frontal circuit (IFC), and genu of corpus callosum (CC-G). Fractional anisotropy (FA) as a measure of WM integrity was compared between actively opioid dependent subjects [OD, N = 30], their non-substance-dependent siblings [SG, N = 30], opioid dependent subjects currently abstinent [AG, N = 15] and non-substance-dependent controls [CG, N = 15] who were gender and handedness-matched. Trait impulsivity and executive functions were also compared. Compared to CG, OD group had significantly lower FA in the bilateral IFC and right OFC. Reduced FA in left IFC was also present in SG, when compared to CG, whereas SG had higher FA in the left anterior cingulum than OD. AG had significantly low FA in left IFC and CCG than CG. Trait impulsivity was highest in OD, followed by SG and CG. Non-planning impulsiveness score was significantly correlated with FA of left IFC. OD had impairment in executive functions compared to CG. WM changes in opioid dependence involve fronto-striatal circuits implicated in poor decisional balance and impulsivity. Changes in Left IFC appear to predate the onset of addiction, conferring vulnerability, and persist during abstinence. Behavioral and neuro-psychological assessments concur with the imaging results.

Dissociation of water on atomically-defined cobalt oxide nanoislands on Pt(111) and its effect on the adsorption of CO

Abstract

Diagnostic accuracy of thromboelastometry and its correlation with the HPLC-MS/MS quantification test.

The aim of the study was to evaluate the diagnostic accuracy of thromboelastometry for assessing rivaroxaban concentrations. The accuracy of thromboelastometry was compared with the high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method, which is the gold standard for drug plasma monitoring (the reference standard). Forty-six clinically stable patients were treated with 10, 15, or 20 mg of rivaroxaban once daily (OD group) or 15 mg twice a day (BID group) (no particular indication for treatment). Patient samples were collected 2 h after the use of the medication (peak) and 2 h before the next dose (trough). The rivaroxaban plasma concentrations were determined via HPLC-MS/MS, and thromboelastometry was performed using a ROTEM® delta analyzer. There were significant prolongations in clotting time (CT) for the 10, 15, and 20 mg of rivaroxaban treatments in the OD groups. In the 15 mg BID group, the responses at the peak and trough times were similar. At the peak times, there was a positive correlation between the plasma concentration of rivaroxaban and CT (Spearman correlation rho=0.788, P<0.001) and clot formation time (rho=0.784, P<0.001), and a negative correlation for alpha angle (rho=−0.771, P<0.001), amplitude after 5 min (rho=−0.763, P<0.001), and amplitude after 10 min (rho=−0.680, P<0.001). The CT presented higher specificity and sensitivity using the cut-off determined by the receiver characteristics curve. ROTEM has potential as screening tool to measure possible bleeding risk associated with rivaroxaban plasma levels.

Absence of Healing Impairment in Osteotomies Prepared via Osseodensification Drilling.

This study sought to qualitatively and quantitatively evaluate the effect of osteotomy preparation by conventional (control group) or OD (OD group) instrumentation on osteotomy healing. An incision of 10 cm was made in the anteroposterior direction over the hip in five sheep, and 15 osteotomies were prepared in the left ilium of the sheep (n = 3/sheep). Three different instrumentation techniques were utilized: (1) conventional/regular drilling (R [recommended by manufacturer]) in a 3-step series of a 2-mm pilot, 3.2-mm, and 3.8-mm twist drills; (2) OD clockwise (OD-CW) drilling with Densah Bur (Versah) 2.0-mm pilot, 2.8-mm, and 3.8-mm multi-fluted tapered burs; and (3) OD counterclockwise (OD-CCW) drilling with Densah Bur 2.0-mm pilot, 2.8-mm, and 3.8-mm multi-fluted tapered burs. Drilling was performed at 1,100 rpm with saline irrigation. Qualitative histomorphometric analysis of the osteotomies after 6 weeks did not show any healing impairment due to the instrumentation. Histologic analysis shows bone remodeling and growth in all samples, irrespective of osteotomy preparation technique, with the presence of bone chips observed along the length of the osteotomy wall in sites subjected to osseodensification drilling.

A two-step immunoassay for the simultaneous assessment of A\u03b238, A\u03b240 and A\u03b242 in human blood plasma supports the A\u03b242/A\u03b240 ratio as a promising biomarker candidate of Alzheimer\u2019s disease

BackgroundThe quantification of amyloid-beta (Aβ) peptides in blood plasma as potential biomarkers of Alzheimer’s disease (AD) is hampered by very low Aβ concentrations and the presence of matrix components that may interfere with the measurements.MethodsWe developed a two-step immunoassay for the simultaneous measurement of the relative levels of Aβ38, Aβ40 and Aβ42 in human EDTA plasma. The assay was employed for the study of 23 patients with dementia of the Alzheimer’s type (AD-D) and 17 patients with dementia due to other reasons (OD). We examined relationships with the clinical diagnosis, cerebral Aβ load as quantified by amyloid-positron emission tomography, apolipoprotein E genotype, Aβ levels and Tau protein in cerebrospinal fluid.ResultsPreconcentration of plasma Aβ peptides by immunoprecipitation substantially facilitated their immunological measurements. The Aβ42/Aβ40 and Aβ42/Aβ38 ratios were statistically significantly lower in the AD-D patients than in the OD group. The areas under the receiver operating characteristic curves reached 0.87 for the Aβ42/Aβ40 ratio and 0.80 for the Aβ42/Aβ38 ratio.ConclusionsThe measurement of plasma Aβ peptides with an immunological assay can be improved by preconcentration via immunoprecipitation with an antibody against the Aβ amino-terminus and elution of the captured peptides by heating in a mild detergent-containing buffer. Our findings support the Aβ42/Aβ40 ratio in blood plasma as a promising AD biomarker candidate which correlates significantly with the validated core biomarkers of AD. Further studies will be needed for technical advancement of the assay and validation of the biomarker findings.

Key pathway and gene alterations in the gastric mucosa associated with obesity and obesity‐related diabetes

The stomach plays an important role in obesity and obesity-related diabetes; yet, little is known about key pathways in the gastric mucosa associated with obesity and diabetes. We performed gene microarray and real time-polymerase chain reaction (RT-PCR) on gut mucosa samples from control subjects (CON), patients with simple obesity (OB), and patients with obesity and comorbid diabetes (OD) (n = 3 per group). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to predict the functional significance of differentially expressed genes. In total, 262 genes were upregulated and 265 genes were downregulated in the OB group whereas 1756 genes were upregulated and 1053 genes were downregulated in the OD group compared with the CON group. Of these, 23 were co-regulated in both comparisons. Seven differentially expressed genes were validated by RT-PCR (NRIP3, L1CAM, TPO, P2RY1, OR8A1, ADAMTS19, and ASIC3). A functional analysis revealed that genes differentially expressed between the OB or OD and CON groups played crucial roles in metabolic, T cell, and G-protein coupled receptor biological processes, and primarily participated in the PI3K-Akt and AGE-RAGE signaling pathways. Obesity and obesity-related diabetes are associated with important gene expression and pathway alterations in the stomach.

Clinical follow-up observation of 84 cases of implant-retained/supported prostheses in edentulous patients

Objectives: To evaluate the long-term clinical results and patient satisfaction of implant-retained/supported prostheses in edentulous patient based on retrospectively summary of the clinical data of implant-retained/supported prostheses patients. Methods: Edentulous patients who received implant denture treatment between 01/2005 and 12/2016 was included in this study. Soft tissue health, clinical examination, X-ray observation, patient satisfaction with implant-retained/supported prostheses were evaluated. Results: A total of 400 implants in 84 cases (96 jaws), 35 fixed bridges and 61 overdentures. The follow-up time was (34.3±25.5) months. The 3- and 5-year implant cumulative survival rate of implant-supported overdenture was 95.0% and 92.1%, and the 3- and 5- year survival rate of implant-supported fixed denture was both 99.9%. The prevalence of peri-implantitis was 2.8% (11/400). A total of 44% (27/61) of the overdenture and 23%(8/35) of the fixed denture were repaired due to the complications of prosthesis. The mean repair frequency was (1.4±1.2) in overdenture and (1.3±0.9) in fixed denture (t=0.490, P=0.600). The average probing depth around implant recorded in the overdenture group [(2.2±1.1) mm] was significantly greater than that in the fixed denture group [(1.4±1.2) mm] (t=3.300, P=0.002). Patient satisfaction with an average of (6.5±4.9) in implant-support overdenture group was significantly higher than that in implant-support fixed denture group (3.2±2.8) (t=3.218, P=0.006). Conclusions: The complication rate of the prosthesis was high in edentulous patients with implant-retained/supported prostheses. Besides, the patients with implant supported denture expressed more satisfaction with their prostheses. And there was a relatively high implant cumulative survival rate of implant-retained/supported prostheses in edentulous patient.

Analysis on Passenger Flow Changes during Holidays—A Case Study of Beijing-Shanghai High-Speed Railway

In China, the number of passengers taking the high-speed train will increase dramatically during the short-term holidays. However, only a few researchers have analysed the characteristics of high-speed railway passenger flow change during the holidays and all of them just focused on the total number change, lacking analysis on different OD types. Therefore, in order to provide specific advice for making and optimizing the holiday high-speed train line plan, this paper studied passenger flow change during holidays based on OD classification, taking Beijing-Shanghai High-Speed Railway (BSHR) as an example. Firstly, we classified OD pairs into different groups according to OD level and OD distance, while the OD level is defined by the station level which was obtained by Hierarchical Clustering Method. Secondly, by counting and calculating the increased number of passengers during each holiday for each OD group, we drew the conclusion that the increased passenger flow of BSHR in holidays is mainly from the 2nd level OD, 1st level OD and [0, 500)km OD. Due to this result, we suggest to increase the service frequency of 1st and 2nd level OD pairs in holidays. Also, we prefer to add short-distance trains and try to avoid adding too many long-distance trains.

Evaluation of antihypertensive efficacy of Losartan + Hydrochlorthiazide versus Telmisartan + Hydrochlorthiazide in patients with stage 1 or stage 2 hypertension: a randomized controlled trial

Background: Cardiovascular disease (CVD) is the most common contributor of morbidity and mortality in underdeveloped and developing countries including the South Asian countries (including India and Pakistan). Amongst the cluster group of CVDs, Hypertension (HTN) represents the most common cardiovascular risk factor. The aim of this trial was to evaluate of antihypertensive efficacy and effect on biochemical parameters of Losartan + Hydrochlorthiazide versus Telmisartan + Hydrochlorthiazide in patients with stage 1 or stage 2 hypertension with a randomized controlled trial.Methods: This was a prospective, randomized controlled trial of Losartan + Hydrochlorthiazide versus Telmisartan + Hydrochlorthiazide in patients with stage 1 or stage 2 hypertension. The primary endpoint on treatment was analysis of antihypertensive efficacy of these drug combinations. The variables were compared at different time points- baseline, 3 and 6 months.Results: In the present study, 76 patients were enrolled with 38 patients each allocated to each treatment groups. The effect of Losartan 50mg + Hydrochlorthiazide 12.5mg OD was found to be significant on SBP and DBP in both supine as well as sitting position than Telmisartan 80mg + Hydrochlorthiazide 12.5mg OD group at 3 and 6 months.Conclusions: The results on anti- hypertensive efficacy was far better in Losartan 50mg + Hydrochlorthiazide 12.5mg OD group than Telmisartan 80mg + Hydrochlorthiazide 12.5mg OD group.

Interventions to Maintain Locator-Retained Mandibular Overdentures on Both External Hex and Internal Connection Implants: A Retrospective Study.

To quantify the rate of maintenance interventions of mandibular prostheses: complete dentures and overdentures retained by two implants, with either internal hex or external hex connections. This study analyzed patients with edentulous mandibles treated with distinct treatment protocols: a control group received complete dentures; a second group received Locator-retained overdentures supported by two external hex connection implants; and a third group received overdentures supported by internal hex connection implants. All of the maintenance interventions associated with the prosthetic treatment after delivery were recorded (starting from 2000 until the final assessment in 2012). Moreover, patient satisfaction was assessed using a 0 to 10 visual analog scale. The final sample was composed of 136 patients: 56 in the control group, 31 in the hex implant overdenture group, and 49 in the internal connection overdenture group followed over a period of 60 months (ranging from 3 to 144 months). Prosthetic adjustment was the most frequent intervention carried out, particularly within the control group (a mean rate of 1.1 ± 1.9 times/subject/year) in comparison with the internal connection (0.6 ± 0.8 times/subject/year) and hex implant overdenture groups (0.3 ± 0.2 times/subject/year). Relining was also frequently required, occurring on average every 4 years in the control group and every 7 years in the overdenture groups. Abutment loosening was more frequent in the hex implant overdenture group than in the internal connection overdenture group (8/31 versus 8/49), as well as abutment wear (5/31 versus 5/49). Gingival inflammation was significantly more common in the hex implant overdenture group (19/31 patients), as compared with the controls (16/56 subjects), and satisfaction was higher in patients in the two overdenture groups (mean Oral Satisfaction Scale [OSS] score of 8.1 ± 1.9 for external and 8.4 ± 1.6 for internal connections) than in the control group (5.3 ± 3.3). Locator-retained overdentures needed less than half the number of maintenance interventions when compared with the control group, and maintenance was required every 2 to 4 years.

Co-occurrence of opisthorchiasis and diabetes exacerbates morbidity of the hepatobiliary tract disease.

Complications arising from infection with the carcinogenic liver fluke Opisthorchis viverrini cause substantial morbidity and mortality in Thailand and adjacent lower Mekong countries. In parallel, the incidence rate of diabetes mellitus (DM) is increasing in this same region, and indeed worldwide. Many residents in opisthorchiasis-endemic regions also exhibit DM, but the hepatobiliary disease arising during the co-occurrence of these two conditions remains to be characterized. Here, the histopathological profile during co-occurrence of opisthorchiasis and DM was investigated in a rodent model of human opisthorchiasis in which diabetes was induced with streptozotocin. The effects of excretory/secretory products from the liver fluke, O. viverrini (OVES) on hepatocyte and cholangiocyte responses during hyperglycemic conditions also were monitored. Both the liver fluke-infected hamsters (OV group) and hamsters with DM lost weight compared to control hamsters. Weight loss was even more marked in the hamsters with both opisthorchiasis and DM (OD group). Hypertrophy of hepatocytes, altered biliary canaliculi, and biliary hyperplasia were more prominent in the OD group, compared with OV and DM groups. Profound oxidative DNA damage, evidenced by 8-oxo-2'-deoxyguanosine, proliferating cell nuclear antigen, and periductal fibrosis characterized the OD compared to OV and DM hamsters. Upregulation of expression of cytokines in response to infection and impairment of the pathway for insulin receptor substrate (IRS)/phosphatidylinositol-3-kinases (PI3K)/protein kinase B (AKT) signaling attended these changes. In vitro, OVES and glucose provoked time- and dose-dependent effects on the proliferation of both hepatocytes and cholangiocytes. In overview, the co-occurrence of opisthorchiasis and diabetes exacerbated pathophysiological damage to the hepatobiliary tract. We speculate that opisthorchiasis and diabetes together aggravate hepatobiliary pathogenesis through an IRS/PI3K/AKT-independent pathway.

Assisted Reproductive Technology, Comorbidities, and Cardiovascular Risk: The Experience of an Italian Center.

A growing number of infertile women undergo assisted reproductive technology (ART). Older maternal age and the presence of comorbidities may affect cardiovascular (CV) women health. Safety aspects are central in risk assessment; we report the experience of internal medicine clinical setting at the ART center. we investigated 243 infertile women (110 autologous and 133 oocyte donation [OD]); cardiometabolic parameters and CV risk factors were investigated during the clinical evaluation. We observed high prevalence of dyslipidemia (62.9%), smoking habit (27.5%), and body mass index (26.7%). Waist ≥80 cm was present in 65%, and waist to hip ratio (WHR) value >0.80 in 60.5% of women. The prevalence of migraine with aura, hypertension, and diabetes was 5.3%, 4.9%, and 4.5%, respectively. By evaluating variables according to autologous or OD ART, we observed that OD women were older than autologous, p < 0.0001. Overweight and obesity prevalence was higher in OD group; waist and WHR were significantly higher in autologous than in OD group (75% vs. 57%, p = 0.005, and 91.8% vs. 34.6%, p = 0.01, respectively). We observed a statistically significant unfavorable risk profile in OD in comparison to autologous women (p = 0.003); in particular, 60% of OD women carried 3-5 CV risk factors, and 8.3% carried at least six CV risk factors. The experience of an internal medicine clinic at the ART center strongly underlies the relevance of a careful medical evaluation before attempting ART, and suggests the opportuneness of a structured internal medicine clinic in every gynecology setting to better frame risk profile.

Water on Atomically-Defined Cobalt Oxide Surfaces Studied by Temperature-Programmed IR Reflection Absorption Spectroscopy and Steady State Isotopic Exchange

In this work, we investigate the interaction of water with three different atomically defined cobalt oxide surfaces under ultrahigh vacuum (UHV) conditions using time-resolved and temperature-programmed infrared reflection–absorption spectroscopy (TR-IRAS, TP-IRAS) in combination with isotopic exchange experiments. The three surfaces, CoO(100), CoO(111), and Co3O4(111), are prepared in form of well-ordered films on Ir(100). Very different behavior is observed on the three surfaces, both with respect to D2O adsorption and desorption. On CoO(100), water adsorbs molecularly. It forms extended ice clusters even at low adsorption temperature (200 K) and which desorb molecularly at 200 K (Ea,des ∼ 60 kJ·mol–1). A very small amount of defect sites is observed at which D2O dissociates and forms strongly bound OD groups. On CoO(111), the interaction with ordered facets is very weak and no adsorption occurs at these sites at 200 K. The CoO(111) films expose, however, a higher density of defects as compared to the C...

Hybrid model predictive control based dynamic pricing of managed lanes with multiple accesses

We propose a hybrid model predictive control (MPC) based dynamic tolling strategy for high-occupancy toll (HOT) lanes with multiple accesses. This approach preplans and coordinates the prices for different OD pairs and enables adaptive utilization of HOT lanes by considering available demand information and boundary conditions. It also addresses such practical issues as prevention of recurrent congestion in HOT lanes, ensuring no higher toll for a closer toll exit and fairness among different OD groups at each toll entry, as well as the fact that high occupancy vehicles (HOVs) have free access to the HOT lanes. Taking the inflows at each toll entry as the control, traffic densities and vehicle queue length as observed system states, and boundary traffic as predicted exogenous input, we formulate a discrete-time piecewise affine traffic model. Optimal tolls are then derived from a one-to-one mapping based on the optimal toll entry flows. By properly formulating the constraints, we show that the MPC problem at each stage is a mixed-integer linear program and admits an explicit control law derived by multi-parametric programing techniques. A numerical experiment is presented for a representative freeway segment to validate the effectiveness of the proposed approach. The results show that our control model can react to demand and boundary condition changes by adjusting and coordinating tolls smoothly at adjacent toll entries and drive the system to a new equilibrium that minimizes the total person delay. Under the optimal prediction horizon, the on-line computational cost of the proposed control model is only about 4% and 8% of the modeling cycle of 30 s, respectively, for two typical traffic scenarios, which implies a potential of real-time implementation.

Blood calcium, glucose and haematology profiles of parturient bitches diagnosed with uterine inertia or obstructive dystocia

Bitches with dystocia most often present with clinical signs of uterine inertia (UI). The aetiology of myometrial dysfunction in most of these cases is still not elucidated. We compared blood ionized calcium (iCa) and glucose concentrations in bitches diagnosed with primary UI (PUI, n=14), secondary UI (SUI, n=6) or obstructive dystocia (OD, n=6), and we described their haematology profiles. Bitches diagnosed with UI had a patent birth canal and delivered no puppies yet (PUI) or only part of the whole litter (SUI). The OD group had no UI and showed strong abdominal contractions. Blood iCa did not differ between the PUI, SUI and OD groups and was not influenced by litter size. There was a significant positive relationship (R2 =.241, p=.013) between iCa concentrations and the dam's body weight. Glucose concentrations were also not significantly different between dystocia groups or influenced by body weight and litter size. Hypocalcaemia was detected in 11 bitches, and hypoglycaemia in two bitches. Pregnancy-associated anaemia was seen in about one-third of the bitches. Eight of 12 dogs had increased platelet counts, and ten had leukocytosis with mature neutrophilia. Although iCa did not differ between dystocia groups, low concentrations may have contributed to the development of UI in some of the small size bitches. Hypoglycaemia was uncommon, and therefore, we consider low glucose concentrations not to have played an important role in the pathogenesis of UI in our study population. Pregnancy-associated anaemia, thrombocytosis, leukocytosis and mature neutrophilia were common findings in otherwise healthy bitches diagnosed with different forms of dystocia.

Decreased Serum Lipid Levels and Ratios Correlate with Low Prevalence of Coronary Heart Disease in Patients with Parkinson's Disease.

Patients with Parkinson's disease (PD) are at a lower risk of suffering cardiovascular events, but the underlying factors for this decreased risk remain unclear. Serum triglycerides (TG) and total cholesterol (TC), and their expression relative to high-density lipoprotein cholesterol (TG/HDL-C and TC/HDL-C), are independent predictors of cardiovascular events. This study aimed to determine if PD patients have decreased lipid levels and lipid ratios, which may underlie the decreased risk of coronary heart disease (CHD). This retrospective study included 92 PD patients (PD group), 450 control subjects with no CHD (OD group), and 450 CHD patients (CHD group). We analyzed serum lipid levels and lipid ratios in each group. There were significant differences in TC (F = 10.459, p < 0.0001), TG (F = 46.856, p < 0.0001), low density lipoprotein cholesterol (LDL-C) (F = 6.910, p = 0.001), high density lipoprotein cholesterol HDL-C (F = 30.694, p < 0.0001), TC/HDL-C (F = 32.675, p < 0.0001), and TG/HDL-C (F = 45.554, p < 0.0001) between all three groups; TC/LDL-C (F = 2.518, p = 0.081) was not significantly different between groups. Compared to the CHD group, PD patients had lower TC (p < 0.0001), TG (p < 0.0001), LDL-C (p = 0.001), TG/HDL-C (p < 0.0001), and TC/HDL-C (p < 0.0001); TC/LDL-C (p = 0.563) and HDL-C (p = 0.196) were not significantly different. TC and LDL-C levels were positively correlated within individual groups (all p < 0.0001). In addition, TG and HDL-C were negatively correlated in the OD and CHD groups (p < 0.0001); no significant negative association was observed in the PD group (p = 0.077). TG/HDL and LDL-C levels were inversely correlated in the CHD group (p < 0.0001) and weakly positively correlated in the PD (p = 0.159) and OD (p = 0.199) groups. TC/HDL and TG/HDL ratios were significantly lower in PD patients compared to CHD patients, suggesting there is a strong correlation between lipid ratios and incidence of CHD in PD patients.

Factors associated with bone metabolism in patients with cervical ossification of the posterior longitudinal ligament accompanied with diffuse idiopathic skeletal hyperostosis.

Introduction: Diffuse idiopathic skeletal hyperostosis (DISH) and ossification of the posterior longitudinal ligament (OPLL) are both characterized as ossification in paravertebral ligaments and sometimes present simultaneously, however, the bone metabolism in patients with cervical OPLL accompanying/not accompanying DISH has not well been studied. Thus, a retrospective analysis was performed to understand any differences in bone metabolism in these patients.Methods: Male patients who underwent surgery for OPLL were divided into two groups based on the presence or absence of DISH (OD and O group, respectively). Patients with cervical spondylosis comprised the control group (CS group). Bone mineral density (BMD) and bone metabolism factors were compared among the groups.Results: The OD and O groups had significantly higher body mass indexes (BMIs) than did the CS group. Morphologically, the number of continuous type of OPLL was high in the OD group whereas that of segmental type was higher in the O group. The OD and O group had greater BMD than the CS group. Both TRACP-5b and P1NP were tended to be lower in the OD group whereas Ca and P concentrations were similar level among the groups. Intact parathyroid hormone in OD group was significantly higher than CS group.Discussion: Patients with OPLL accompanying DISH had significantly higher BMD whereas they tend to be lower in bone turnover. Significantly higher i-PTH levels was found in the OD group and would be the characteristic blood marker, but further research on the relationship between DISH and PTH was necessary.

Characteristics of empathy and psychopathy among patological Internet users and opiate-dependent persons

Background/Aim. The debate about diagnostic criteria for differences and similiraties as psychosocial characteristics of pathological Internet users and Internet and substance addicts still continued. The aim of the study was to investigate the differences between empathy and psychopathy among subjects with pathological Internet use (PIU) and opiate dependence. Methods. The cross sectional study was performed at the Methadone Center of the Clinical Centre of Vojvodina, Novi Sad, Serbia, with 40 consecutively recruited patients with opiate dependence (OD group) and compared to age matched (25 to 35 years) random online recruited subjects (n = 80). They were divided into two groups: 40 subjects with PIU and 40 controls according to cut-off score of 39 or less obtained at Questionnaire regarding the Internet use assessment. The questionnaires for sociodemographic data, Emotion Specific Empathy (EMI) questionnaire and Psychopathy Assessment Questionnaire (PAQ) were applied. Descriptive statistics, _2-tests, ANOVA and MANOVA analysis were used for data assessment which was performed by the SPSS software (version 20.0) and p &lt; 0.05 was applied. Results. There were more differences among psychopathy dimensions than empathy dimensions among PIU subjects, controls and heroin dependent subjects. The PIU group demonstrated the lowest psychopathy dimensions except for the lifestyle dimension which was higher than in the control group. On the contrary, the PIU group showed the highest empathy, with two dimensions as the most prominent ones: empathy with negative emotions and emotional reactions provoked by empathy. Conclusion. The PIU subjects are characterized by high empathy and low psychopathy. These findings suggest that it would be useful to consider seriously the dimensions of empathy and psychopathy when developing strategies to prevent PIU and planning educational programs for persons with problems related to the Internet overuse disorder.

Effect of orlistat or metformin in overweight and obese polycystic ovary syndrome patients with insulin resistance

The aim of this study was to evaluate the effect of orlistat or metformin combined with Diane-35 on anthropometric, hormonal and metabolic parameters in overweight and obese polycystic ovary syndrome (PCOS) patients with insulin resistance (fasting insulin > 10 mIU/L). A total of 240 PCOS women were randomly allocated to orlistat plus Diane-35(OD group), metformin plus Diane-35(MD group), orlistat plus metformin plus Diane-35(OMD group) or Diane-35 (D group). Body weight, BMI, waist and hip circumference, blood pressure, endocrine profile, lipid profile and insulin resistance were assessed at baseline and after 3 months. Significant reductions in waist and hip circumference, serum LH, total testosterone and uric acid were observed in all groups compared with baseline. TG and TC significantly decreased in the OD group. Homeostasis model assessment insulin resistance (HOMA-IR) index was reduced in the OD (p = .015), MD (p = .001) and OMD (p = .004) groups. Body weight, BMI, systolic BP and HDL-C significantly changed in the OD and OMD group compared with the D group (p < .05). Side effects were less with orlistat than metformin. This study demonstrated that orlistat is more effective in reducing weight and lipid profile than metformin. Besides, orlistat has mild side-effects and is better tolerated compared with metformin.

Spectroscopic and Quantum Mechanical Calculation Study of the Effect of Isotopic Substitution on NIR Spectra of Methanol.

In this work, we studied methanol and its deuterated derivatives (CH3OH, CH3OD, CD3OH, CD3OD) by NIR spectroscopy and anharmonic quantum chemical calculations. Vibrational bands corresponding to up to three quanta transitions (first and second overtones, binary and ternary combination modes) were predicted by the use of the VPT2 route. The accuracy of prediction of NIR modes was evaluated through density functional theory (DFT) with selected density functionals and basis sets. On the basis of the theoretical NIR spectra, detailed band assignments for all studied molecules were proposed. It was found that the pattern of bands in NIR spectra of deuterated methanols can be used for identification of isotopically equalized forms. Calculations of NIR spectra of all possible forms of CXXXOX (X = H, D) molecules demonstrated that the isotopic contamination can be identified due to a coexistence of bands specific to OH and OD groups. Also, bands from partially deuterated methyl groups can be distinguished in NIR spectra. Since the VPT2 framework is known to be sensitive to inaccuracy in the case of highly anharmonic modes, we obtained an independent insight by numerical solving of the time-independent Schrödinger equation corresponding to the O-X stretching mode scanned within -0.4 to 2.0 Å over a dense grid of 0.005 Å. This way the energies of vibrational levels of the CX1X2X3OX4 (X = H, D) isotopomers and the corresponding transition frequencies were obtained with high accuracy (<0.1 cm-1). The change in normal coordinate influences the reduced mass of the oscillator and thus its frequency. Our results lead to a conclusion that the effect of deuterization of the methyl group introduces a very specific and consistent frequency shift of the first overtone of the O-X stretching mode depending on the substitution of X1, X2, or X3 positions (<2 cm-1). However, the pattern of this shift is not reproduced accurately and is also largely overestimated by VPT2 calculations.