Climate, growth promoting hormones and soil nutrition are all necessary conditions for the production of any crop. The primary job is also to determine the ideal planting period for optimum crop yield. In order to improve strawberry growth, yield, and quality parameters a field experiment was carried out to evaluate the comparative study of planting time, growth regulator and biofertilizer on growth, yield and quality of strawberry cv Chandler at Horticultural Research Center, Chauras Campus, H.N.B. Garhwal University, Srinagar Garhwal, Uttarakhand, India, during two growing seasons 2018-19 and 2019-20. The present experiment consists of three factors with a total of 08 treatments which was replicated thrice on strawberry cultivar Chandler at different planting time viz., 15th August, 5th September, 25th September and 15th October with the application of various GA3 concentration viz., no GA3 (without application), 50 ppm GA3, 75 ppm GA3, 100 ppm GA3 and with and without treatment with Azotobacter. The treatment under15th October planting time, 75 ppm GA3 concentration with Azotobacter was found most effective for most of the growth, yield and quality traits viz.,plant height (34.57 cm), leaf area (104.66 cm2), number of runners/plant (8.60), days taken to full blooming (202.00), fruit setting % (89.17), days taken to final harvest (214.44), fresh weight of fruit (15.93 g), fruit yield/plant (327.09 g), TSS (9.07 0Brix), vitamin C (67.70 mg/100g), sugar content (7.08%) and juice content (91.06%) whereas the minimum values for most of the characters was observed with 15th August planting time and without application of GA3 and Azotobacter treatment.