A dynamic chiral stationary phase (CSP) prepared by hydrophobically bonding N-dodecyl diamide of (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid to octadecyl silica gel was successfully employed in resolving various racemic compounds containing a primary amino functional group. The racemic compounds resolved on the CSP include α-amino acids, α-amino esters, amino alcohols, amines, α-aminocarbonyl compounds, and fluoroquinolone antibacterial agents. In order to see the effect of organic and acidic modifiers in the mobile phase and the temperature of the column on the enantioselectivity exerted by the CSP, five racemic compounds that resolved well on the CSP were selected and their resolutions were investigated with the variation of organic and acidic modifiers in the mobile phase and the temperature of the column. The results demonstrate that the chromatographic factors such as capacity factors (k'), separation factors (α), and resolution factors (RS) might be controlled, to some extent, by varying organic and acidic modifiers in the mobile phase and the temperature of the column.