Ochrobactrum Anthropi Research Articles

Acute endophthalmitis caused by Ochrobactrum anthropi following cataract surgery in an immunocompetent patient – A case report

A 54-year-old man developed left eye inflammation three days after cataract surgery and was diagnosed with endophthalmitis. Aqueous culture revealed Ochrobactrum anthropi resistant to vancomycin and cephalosporin. Systemic and intravitreal ciprofloxacin injections caused only transient improvement and eventually, pars plana vitrectomy was done. There was complete resolution, good visual recovery, and no recurrence. O.anthropi is an uncommon organism with a characteristic resistance to empirical antibiotics and usually causes chronic infection in immunocompromised. We report a rare case of acute endophthalmitis in an immunocompetent patient. To the best of our knowledge, this is the first report of O. anthropi endophthalmitis from India.

Ochrobactrum anthropi: A Rare Cause of Culture-Proven Acute Post-Operative Cluster Endophthalmitis

ABSTRACT Aim: To report the clinical presentation and management outcomes of an outbreak of culture-proven post-operative endophthalmitis (POE) secondary to Ochrobactrum anthropi. Methods: This study was conducted at a tertiary-care eye hospital in South India. Results: Fifty-five patients underwent cataract surgery by six surgeons on three consecutive days in the same surgical facility in September 2020. Four patients developed POE after a mean interval of 12.0 ± 4.8 days and underwent immediate vitreous tap and intravitreal antibiotic injections (Vancomycin and Ceftazidime). All patients required a core vitrectomy (culture positive, n = 4/4). Two patients underwent an additional intraocular lens explantation, 2 and 4 months after presentation. All the four patients showed good anatomical and functional recoveries (mean follow-up, 5.75 ± 1.5 months). Two other patients had an exaggerated post-operative inflammation, which was successfully treated with topical medications. Conclusions: O. anthropi is a rare cause of acute POE. Although the isolated organism was multi-drug resistant, the outcome was good in all the patients.

Hydrogen sulfide affects the performance of a methanogenic bioelectrochemical system used for biogas upgrading

Methanogenic bioelectrochemical systems (BESs) can convert carbon dioxide (CO2) to methane (CH4) and may be used for anaerobic digester biogas upgrading. However, the effect of hydrogen sulfide (H2S), a common biogas component, on BES performance is unknown. Thus, the objective of this study was to assess the effect of H2S addition to the cathode headspace on BES performance at a range of initial gas-phase H2S concentrations (0–6% v/v), as well as its effect on the anode and cathode microbial communities. As the initial cathode headspace H2S increased from 0 to 2% (v/v), biocathodic CH4 production increased by two-fold to 3.56 ± 0.36 mmol/L-d, due to dissolved H2S transport from the cathode to the anode where H2S was oxidized. Elemental sulfur and sulfate were H2S oxidation products detected in the anode. Above 3% initial cathode headspace H2S, biocathodic CH4 production declined due to inhibition. A phylotype most closely related to Methanobrevibacter arboriphilus dominated the cathode archaeal communities. In the sulfide-amended BES, a phylotype similar to the exoelectrogen Ochrobactrum anthropi was enriched in both the anode and cathode, whereas phylotypes related to sulfate-reducing and sulfur oxidizing Bacteria were detected in the bioanode. Thus, sulfide transport and oxidation in the anode play an important role in methanogenic BESs treating sulfide-bearing biogas.

Preparation of collective lead-zinc concentrates for selection cycle

The object of the study is a collective concentrate, which was obtained by flotation of sulfide lead-zinc ore from an East Siberian deposit using a combination of diesel fuel and butyl xanthate. In the collective concentrate the main ore minerals are galena and sphalerite. Non-metallic minerals are quartz, dolomite, calcite and chlorite. In the work were studied various methods of preparing a collective concentrate for a flotation selective cycle: without pulp preparation, washing with sodium sulfide, temperature, ultrasound, and bacterial treatment of a collective concentrate. The results of studies of the flotation selective cycle showed that it is impossible without preparation. Satisfactory technological indicators were not obtained when applying twice washing with sodium sulfide and using ultrasound. Introduction to the flow sheet of the operation of steaming in a medium of sodium sulfide and dosing of activated carbon into the process made it possible to obtain a foam product (lead concentrate) with a lead content of 45%, but the recovery was ~ 43%. In addition, the process is environmentally unfavorable, characterized by high material and energy costs. The prospects of using the bacterial method for preparing collective concentrates using diesel fuel for the flotation selective cycle are shown. The bacterial method consists in treating the collective concentrate with the bacteria Ochrobactrum anthropi and Pseudomonas aeruginosa JCM 5962.

Identification of Cellulolytic Bacteria from Guts of Microcerotermes diversus Silvestri (Isoptera: Termitidae) in Southern Iran.

Termites are a large and important group of insects in terrestrial ecosystems that decompose lignocelluloses. Among these, Microcerotermes diversus Silvestri (Termitidae) is a destructive invasive pest in many tropical and subtropical regions. In the present study, M. diversus specimens were collected from traps in Ahvaz, Bandar Abbas, Kish, and Khark Islands. Sample suspensions were prepared in 5-ml DH2O and cultured on nutrient agar (NA) medium. All 47 representative bacterial isolates were evaluated for cellulolytic activity by growing them on cellulose Congo-red agar medium. Based on some key phenotypic characteristics, the isolates were tentatively identified at the genus level. These were confirmed by 16S rRNA analysis using a universal primer pair (P1/P6). Sequence alignments revealed that most of the isolates are novel species so far found in the termite guts. Results showed that some of the isolates are common for all surveyed areas. However, there were significant differences in their numbers and degree of cellulolytic activity. The species reported here for the first time for termites of Iran are Bacillus wiedmanii, B. paramycoides, Elizabethkingia anophelis, Lysinibacillus pakestanensis, Pseudomonas hibiscicola, Actinetobacter pitti, A. venetianus, and Ochrobactrum anthropi.

Antagonistic Effects of Delia antiqua (Diptera: Anthomyiidae)-Associated Bacteria Against Four Phytopathogens

Recent studies have revealed multiple roles of insect-associated microbes such as lignin degradation, entomopathogen inhibition, and antibiotic production. These functions improve insect host fitness, and provide a novel source of discovering beneficial microbes for industrial and agricultural production. Previously published research found that in the symbiosis formed by the dipteran pest Delia antiqua (Meigen) (Diptera: Anthomyiidae) and its associated bacteria, the bacteria showed effective inhibition of one fungal entomopathogen, Beauveria bassiana. The antifungal activity of those associated bacteria indicates their potential to be used as biocontrol agents for fungal phytopathogens. In this study, we first isolated and identified bacteria associated with D. antiqua using a culture-dependent method. Second, we tested the antifungal activity of these bacteria against four phytopathogens including Fusarium moniliforme, Botryosphaeria dothidea, and two Fusarium oxysporum strains using the dual-culture method. In total, 74 species belonging to 30 genera, 23 families, eight classes, and four phyla were isolated and identified. Among those bacteria, Ochrobactrum anthropi, Morganella morganii, Arthrobacter sp. 3, and Acinetobacter guillouiae showed significant volatile inhibition activity against F. moniliforme, B. dothidea, and both F. oxysporum, respectively. Moreover, bacteria including Rhodococcus equi, Leucobacter aridicollis, Paenibacillus sp. 3, and Lampropedia sp. showed significant contact inhibition activity against F. moniliforme, B. dothidea, and both F. oxysporum. Our work provides a new source for discovering biocontrol agents against phytopathogens.

Efficient reduction of reactive black 5 and Cr(Ⅵ) by a newly isolated bacterium of Ochrobactrum anthropi

It is important to obtain bacteria with the ability for reduction of dyes and Cr(Ⅵ) since dyes and Cr(Ⅵ) are often co-exist in textile wastewater. In this study, a new strain belonging to Ochrobactrum anthropi was isolated from textile wastewater, and could efficiently reduce Reactive Black 5 (RB 5) and Cr(Ⅵ). The results showed the degradation efficiency of RB 5 could achieve 100% and reduction efficiency of Cr(Ⅵ) was up to 80% within 3 days at initial RB 5 and Cr(Ⅵ) concentration of 400 mg/L and 20 mg/L. Mn2+ and Cu2+ could enhance the removal of RB 5 and Cr(Ⅵ), respectively. Glycerin, as electron donor, improved reduction efficiencies of RB 5 and Cr(Ⅵ). In addition, reduction mechanisms were further investigated. The results demonstrated that decreasing of RB 5 and Cr(Ⅵ) concentration were mainly through extracellular bioreduction rather than by adsorption. The FTIR and XPS analyses revealed that the O‒H, C‒C and C‒H groups on the cell surface might be involved in the reduction of RB 5 and Cr(Ⅵ). The information gives useful insights into understanding of how the bacterium reduce RB 5 and Cr(Ⅵ). The results indicated that the strain had excellent application prospect for treating industrial wastewater.

Bioremoval of graffiti using novel commercial strains of bacteria

Previous studies have provided evidence that bioremediation deals a novel approach to graffiti removal, thereby overcoming well-known limitations of current cleaning methods. In the present study eight bacteria aerobic, mesophilic and culturable from the American ATCC and the German DSMZ collections of microorganisms, some isolated from car paint waste, colored deposits in a pulp dryer and wastewater from dye works, were tested in the removal of silver and black graffiti spray paints using immersion strategies with glass slides. Absorbance at 600 nm and live/dead assays were performed to estimate bacterial density and activity in all samples. Also, pH and dissolved organic carbon (DOC) and inorganic carbon (DIC) measurements in the liquid media were made, as well as, thickness, colorimetric and infrared (FTIR) spectroscopy measurements in graffiti paint layers were used to evaluate the presence of the selected bacteria in the samples and the graffiti bioremoval capacity of bacteria. Data demonstrated that of the eight bacteria studied, Enterobacter aerogenes, Comamonas sp. and a mixture of Bacillus sp., Delftia lacustris, Sphingobacterium caeni, and Ochrobactrum anthropi were the most promising for bioremoval of graffiti. According to significant changes in FTIR spectra, indicating an alteration of the paint polymeric structure, coupled with the presence of a consistent quantity of live bacteria in the medium as well as a significant increase of DIC (a measure of metabolic activity) and a change in paint color.

Field evaluation of consortium of bacterial inoculants producing ACC deaminase on growth, nutrients and yield components of rice and wheat

Bacterial isolates Achromobacter, Ochrobactrum Pseudomonas, and Variovorax have often been isolated from plant roots, and most of them have been described as growth enhancing rhizobacteria. These rhizobacteria have been used to boost crops productivity in varied region of agro-climatic regions. To lessen the usage of chemical fertilizers inputs and improve the sustainability of rice–wheat crops, this study was planned to investigate the impacts of bacterial consortium inoculants producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase on plant growth, nutrient content and grain yield in a field trial. Rice and wheat plants subjected to plant growth-promoting rhizobacteria (PGPR) consortia of strain DPC9 (Ochrobactrum anthropi) + DPB13 (Pseudomonas palleroniana) + DPB15 (Pseudomonas fluorescens) + DPB16 (Pseudomonas palleroniana) gave the best results with reference to macronutrient—nitrogen, phosphorus, potassium, calcium and sodium content and yield-related parameters including 1000 grain weight (10.2%, 40.7%), number of grains per panicle/spike (45.5%, 60.6%), and tillers (32.2%, 106.6%). This inoculants significantly increased grain yield (65.6%, 74.4%), straw yield (26.8%, 36.9%) and harvest index (14.9%, 13.8%) of rice–wheat crops, respectively, when compared to their counterpart control plants. Significant positive correlation in parameters which contribute in producing high grain yield in rice–wheat include plant height (r = 0.938, r = 0.852), tillers (r = 0.968, r = 0.881), panicle/spike length (r = 0.844, r = 0.912), number of grains per panicle/spike (r = 0.969, r = 0.815), 1000 grain weight (r = 0.833, r = 0.931), straw yield (r = 0.920, r = 0.918) and harvest index (r = 0.909, r = 0.847) and also surge nutrient enhancement in rice and wheat were observed in consortium treated plants. It can be concluded that consortium of DPC9 + DPB13 + DPB15 + DPB16-producing ACC deaminase can serve as a useful bio-inoculant for sustainable rice–wheat production in diverse agro-ecosystem.

Effective Identification of Ochrobactrum anthropi Isolated from Clinical Specimens

Ochrobactrum anthropi는 oxidase를 생산하는 비발효산소성 그람음성 막대균으로 외관이 비슷하고 oxidase가 양성인 비발효 세균과 혼합배양 시 구분이 힘들고 생화학적 동정 장비로는 정확한 동정에 한계가 있다. 따라서 본 연구에서는 생화학적 검사 방법으로 동정이 힘든 세균동정의 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform (MALDI-TOF) 법의 유용성을 제시하고자 하였다. MicroScan을 이용해 검사했던 O. anthropi 5례를 분석한 결과, 최종보고까지 6.2일이 소요되었으며 E. coli의 3.0일에 비해 3.5일이 더 소요되었다. 5번 환자 고름 검체는 Achromobater xylosoxidans와 혼합감염으로 여러 번의 계대 배양과 재검사로 인해 11.3일이 소요되었는데, MALDITOF법으로 검사한 경우 한 번에 동정되었다. 4명의 환자는 기저질환이 있는 60세 이상이었고 기회감염과 원내감염의 가능성을 배제할 수 없었으며, 그 중 92일 만에 채취된 검체는 imipenem과 meropenem에 내성이었다. 따라서 O. anthropi 처럼 동정이 까다로운 세균은 신속하고 적절한 환자 치료를 위해 MALDI-TOF법을 이용한 검사가 매우 유용할 것으로 사료된다.

In vivo production of entomopathogenic nematodes suppressed by asymptomatic bacterial contaminants

Summary Entomopathogenic nematodes (EPN) are excellent biological control agents possessing recycling ability as one of their major attributes. We report the presence of asymptomatic bacteria that can lead to disrupted or low progeny production in Heterorhabditis indica. In a one-to-one in vitro competitive bioassay with contaminants associated with H. indica cuticle, there was a significant suppression in the growth of Sphingomonas koreensis when stressed with the nematode symbiont Photorhabdus luminescens; however, P. luminescens was suppressed when sandwiched between Ochrobactrum anthropi. Bacillus bombysepticus associated with laboratory-reared Galleria when stressed by P. luminescens was significantly suppressed, but not so in the reverse assay. Both O. anthropi and B. bombysepticus were found to be insecticidal to Galleria larvae when fed orally. Tripartite interactive studies on the growth and multiplication of H. indica-P. luminescens symbionts in Galleria larvae, predisposed to S. koreensis, revealed no significant difference initially in the hermaphrodite formation, but subsequently there was a significant decline in the formation of amphimictic females and the final production of infective juveniles. In in vitro studies, none of the contaminants supported the growth and development of axenic H. indica. Adequate precautions should be taken to maintain proper hygiene to eliminate such contaminants while culturing the Galleria and EPN for use in the biological control of insects.

Cellular strategies against metal exposure and metal localization patterns linked to phosphorus pathways in Ochrobactrum anthropi DE2010

Cytotoxic, chemical, biochemical, compositional, and morphometric responses were analyzed against heavy metal exposure in Ochrobactrum anthropi DE2010, an heterotrophic bacterium isolated from Ebro Delta microbial mats (Tarragona, NE Spain). Several parameters of effect and exposure were evaluated to determine tolerance to a range of cadmium (Cd), lead (Pb(II)), copper (Cu(II)), chromium (Cr(III)), and zinc (Zn) concentrations. Additionally, removal efficiency, polyphosphate production and metal localization patterns were also analyzed. O. anthropi DE2010 showed high resistance to the tested metals, supporting concentrations of up to 20 mM for Zn and 10 mM for the rest of the elements. The bacterium also demonstrated a high removal capacity of metals—up to 90 % and 40 % for Pb(II) and Cr(III), respectively. Moreover, polyphosphate production was strongly correlated with heavy metal concentration, and three clear cell localization patterns of metals were evidenced using compositional and imaging techniques: (i) extracellular in polyphosphate granules for Cu(II); (ii) in periplasmic space forming crystals with phosphorus for Pb(II); and (iii) intracytoplasmic in polyphosphate inclusions for Pb(II), Cr(III), and Zn. The high resistance and metal sequestration capacity of O. anthropi DE2010 both highlight its great potential for bioremediation strategies, especially in Pb and Cr polluted areas.

Occurrence of the genes encoding carbapenemases, ESBLs and class 1 integron-integrase among fermenting and non-fermenting bacteria from retail goat meat.

The present study was planned to detect the genes encoding carbapenemases, ESBLs and class 1 integron-integrase among bacteria obtained from retail goat meat. Fermenting and non-fermenting bacterial isolates (n=57), recovered from 61 goat meat samples, were identified by 16S rRNA gene sequencing. Antimicrobial susceptibility of isolates was tested by the broth dilution method using ceftazidime, cefotaxime, meropenem and imipenem. Plasmids were isolated and tested for their physical characters. Plasmids were subjected to screening of carbapenemase, ESBL and intI1 gene. Conjugation assay was performed using blaNDM -positive isolates as the donor, and Escherichia coli HB101 as the recipient. Isolates showed the high rates of resistance to ceftazidime (77·2%), cefotaxime (70·2%), meropenem (22·8%) and imipenem (17·5%). They showed variability in number and size (~1 to >20kb) of plasmids. Among all, 1, 4, 13 and 31 isolates showed the blaKPC , blaNDM , blaSHV and blaTEM genes, respectively. The blaKPC-2 gene was observed in one E. coli isolate. The blaNDM-1 gene was detected in Stenotrophomonas maltophilia (n=2), Acinetobacter baumannii (n=1) and Ochrobactrum anthropi (n=1) isolates. These isolates co-harboured the blaTEM and blaSHV genes. The intI1 gene was detected in 22 (38·6%) isolates, and 16 of these isolates showed the carbapenemase and/or ESBL genes. The conjugative movement of blaNDM gene could not be proved after three repetitive mating experiments. The presence of genes encoding carbapenemases and ESBLs in bacteria from goat meat poses public health risks.

Evaluating the Effect of Azole Antifungal Agents on the Stress Response and Nanomechanical Surface Properties of Ochrobactrum anthropi Aspcl2.2.

Azole antifungal molecules are broadly used as active ingredients in various products, such as pharmaceuticals and pesticides. This promotes their release into the natural environment. The detailed mechanism of their influence on the biotic components of natural ecosystems remains unexplored. Our research aimed to examine the response of Ochrobactrum anthropi AspCl2.2 to the presence of four azole antifungal agents (clotrimazole, fluconazole, climbazole, epoxiconazole). The experiments performed include analysis of the cell metabolic activity, cell membrane permeability, total glutathione level and activity of glutathione S-transferases. These studies allowed for the evaluation of the cells’ oxidative stress response to the presence of azole antifungals. Moreover, changes in the nanomechanical surface properties, including adhesive and elastic features of the cells, were investigated using atomic force microscopy (AFM) and spectrophotometric methods. The results indicate that the azoles promote bacterial oxidative stress. The strongest differences were noted for the cells cultivated with fluconazole. The least toxic effect has been attributed to climbazole. AFM observations unraveled molecular details of bacterial cell texture, structure and surface nanomechanical properties. Antifungals promote the nanoscale modification of the bacterial cell wall. The results presented provided a significant insight into the strategies used by environmental bacterial cells to survive exposures to toxic azole antifungal agents.

Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010

The resistance of microorganisms to heavy metals in polluted environments is mediated by genetically determined mechanisms. One such mechanism includes the intracellular sequestration of heavy metals in polyphosphate (polyP) inclusions. In Cr(III) contaminated mediums, Ochrobactrum anthropi DE2010 is able to bind and sequester Cr(III) in polyP inclusions. In order to further study the relationship between Cr(III) tolerance and polyP production in O. anthropi DE2010, we carried out whole genomic sequencing, analysis of single nucleotide polymorphisms (SNPs), polyP chemical quantification, and determination of the relative abundance and morphometry of polyP inclusions. In the O. anthropi DE2010 genome, six polyP and pyrophosphate (PPi) metabolic genes were found. Furthermore, genomic analysis via SNPs calling revealed that O. anthropi ATCC49188 and DE2010 strains had average variations of 1.51% in their whole genome sequences and 1.35% variation associated with the principal polyP metabolic gene cluster. In addition, the accumulation of polyP in the DE2010 strain and number of polyP inclusions found were directly correlated with the concentration of Cr(III) in contaminated cultures. The results presented in this study may enhance the understanding of polyP production in response to Cr(III) toxicity in the O. anthropi DE2010 strain. This knowledge may facilitate the successful removal of Cr(III) from the natural environment.

Kinetics and New Mechanism of Azoxystrobin Biodegradation by an Ochrobactrum anthropi Strain SH14.

Azoxystrobin is one of the most popular strobilurin fungicides, widely used in agricultural fields for decades.Extensive use of azoxystrobin poses a major threat to ecosystems. However, little is known about the kinetics and mechanism of azoxystrobin biodegradation. The present study reports a newly isolated bacterial strain, Ochrobactrum anthropi SH14, utilizing azoxystrobin as a sole carbon source, was isolated from contaminated soils. Strain SH14 degraded 86.3% of azoxystrobin (50 μg·mL−1) in a mineral salt medium within five days. Maximum specific degradation rate (qmax), half-saturation constant (Ks), and inhibition constant (Ki) were noted as 0.6122 d−1, 6.8291 μg·mL−1, and 188.4680 μg·mL−1, respectively.Conditions for strain SH14 based azoxystrobin degradation were optimized by response surface methodology. Optimum degradation was determined to be 30.2 °C, pH 7.9, and 1.1 × 107 CFU·mL−1 of inoculum. Strain SH14 degraded azoxystrobin via a novel metabolic pathway with the formation of N-(4,6-dimethoxypyrimidin-2-yl)-acetamide,2-amino-4-(4-chlorophenyl)-3-cyano-5,6-dimethyl-pyridine, and 3-quinolinecarboxylic acid,6,8-difluoro-4-hydroxy-ethyl ester as the main intermediate products, which were further transformed without any persistent accumulative product. This is the first report of azoxystrobin degradation pathway in a microorganism. Strain SH14 also degraded other strobilurin fungicides, including kresoxim-methyl (89.4%), pyraclostrobin (88.5%), trifloxystrobin (78.7%), picoxystrobin (76.6%), and fluoxastrobin (57.2%) by following first-order kinetic model. Bioaugmentation of azoxystrobin-contaminated soils with strain SH14 remarkably enhanced the degradation of azoxystrobin, and its half-life was substantially reduced by 95.7 and 65.6 days in sterile and non-sterile soils, respectively, in comparison with the controls without strain SH14. The study presents O. anthropi SH14 for enhanced biodegradation of azoxystrobin and elaborates on the metabolic pathways to eliminate its residual toxicity from the environment.

Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.

Quantum Healing- A New Holistic Insight

In that study, a total of 22 sea and mangrove samples from the State of Veracruz were isolated and tested for antimicrobial sensitivity with 9 different antibiotics. The objective was to determine the minimum inhibitory concentration using the modified E-test technique. Phenotypic susceptibility tests revealed that the antibiotics with the highest range of action were Levofloxacin with a total of 77% inhibition of total strains, ciprofloxacin with 81%, sulfametazone with 81% and tetracycline was the most antibiotic antimicrobial activity presented with 90%. This provides information on resistance in non-clinical sample bacteria. 3 bacterial species were identified, being: Staphylococcus aureus, Bacillus thuringiensis, Ochrobactrum anthropi.

Phylogenetic analysis of hydrocarbon degrading bacteria associated with crude oil polluted soil from Mesogar community, Delta State, Nigeria

This study was carried out to isolate hydrocarbon-degrading bacteria associated with oil polluted soil samples collected from Mesogar community of Delta State, Nigeria. The samples were aseptically collected and the bacteria isolated according to standard microbiological techniques. The isolates with hydrocarbon biodegradative ability were screened on MSM supplemented with 2% crude oil using spectrophotometric method. The amount of crude oil degraded by the highest hydrocarbon degrader was determined using gas chromatographic (GC) assay. A total of seven bacterial isolates were molecularly identified using the 16S rRNA gene sequencing method. The sequences were compared to those deposited in NCBI using the basic local alignment tool (BLAST) algorithm. Phylogenetic analysis of 16S rRNA gene sequences was carried out to determine the evolutionary relationships of the isolated bacterial species. The isolates were identified to have remote similarities with Alcaligenes faecalis SH179a, Alcaligenes faecalis subsp. Phenolicus, Bacillus thuringiensis serovar konkukian, Ochrobactrum anthropi, Alcaligenes faecalis SH179b, Uncultured soil bacterium clone and Alcaligenes faecalis IVN45. Strain OFBR 4 had the highest degrading ability. The use of molecular methods for rapid and accurate detection of diverse strains of hydrocarbon-degraders is of utmost necessity in bioremediation.
 Keywords: Phylogenetic analysis, oil polluted soil, biodegradative, hydrocarbon degrader, 16S rRNA gene and crude oil.

Determination of The Antimicrobial Sensitivity of Isolated Sea and Mangrove Bacteria in The State of Veracruz, Mexico, Using the Modified E-Test Technique

In that study, a total of 22 sea and mangrove samples from the State of Veracruz were isolated and tested for antimicrobial sensitivity with 9 different antibiotics. The objective was to determine the minimum inhibitory concentration using the modified E-test technique. Phenotypic susceptibility tests revealed that the antibiotics with the highest range of action were Levofloxacin with a total of 77% inhibition of total strains, ciprofloxacin with 81%, sulfametazone with 81% and tetracycline was the most antibiotic antimicrobial activity presented with 90%. This provides information on resistance in non-clinical sample bacteria. 3 bacterial species were identified, being: Staphylococcus aureus, Bacillus thuringiensis, Ochrobactrum anthropi.