Abstract
The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Highlights
Crude oil contains light and heavy oil fractions of total petroleum hydrocarbon (TPH)
Soil Sampling Soils contaminated by heavy oil waste (HOW) were taken from crude oil contaminated soils, and the HOW was provided by an oil and gas company at North Sumatera, Indonesia
Each isolated polyaromatic hydrocarbons (PAHs) degrader were tested to growth on media containing of phenanthrene (Phe), dibenzothiophene (Dbt), and fluorene (Flr) compounds as carbon sources
Summary
Crude oil contains light and heavy oil fractions of total petroleum hydrocarbon (TPH). Heavy oil waste (HOW) contains polyaromatic hydrocarbons (PAHs). PAHs are relatively stable and recalcitrant in soil. Petrogenic PAHs are often marked as in abundance of alkyl substituted PAHs, such as alkyl naphthalenes, alkyl phenanthrenes, and alkyl dibenzothiophenes (Jong-Su et al 2009). The content of hydrocarbons in the oil-polluted environment is relatively higher than the normal environment. In these circumstances, the survival microbes are that they capable to change the biochemical and molecular processes as an adaptive response to surrounding environmental changes. Little research in Indonesia was done to investigate potential bacteria for PAHs bioremediation. The bioremediation process of heavy oil waste could be done using a bacterial
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