Objective: We aimed to explore the effects of transforming growth factor-β1 (TGF-β1) and concentrated growth factor (CGF) on growth, proliferation and osteogenic differentiation of bone marrow stem cells (BMSCs). Methods: CGF was extracted from rat venous blood. BMSCs was isolated from rats. TGF-β1 or CGF was used to induced BMSCs to observe their effects on BMSCs. The expression levels of BMSCs surface antigens were detected by flow cytometry. ELISA was performed to test the expression of VEGF, TGF-β1, PDGF and SDF-1 in BMSCs. qRT-PCR was carried out to test the expression of COL-I, OPN, OC and ALP in BMSCs. Transwell assay was used to detect the migration rates of BMSCs. The cell morphology of BMSCs was detected by Alizarin red staining. Results: BMSCs were successfully isolated from rats. CGF could significantly increase the expression of TGF-β1, PDGF and VEGF in BMSCs. In addition, TGF-β1 and CGF notably increased the proliferation of BMSCs. And they not only improved the activity of ALD, but also increased the levels of ALP, OPN, COL-I and OCN in BMSCs to prompt the growth of BMSCs. Conclusion: CGF combined with TGF-β1 could prompt the growth, proliferation and osteogenic differentiation of BMSCs.