Mechanism of Bone Marrow Mesenchymal Stem Cells Promoting Migration Ability In Vitro During Traumatic Fracture Healing in Rats

Abstract

Traumatic fractures are clinically common orthopaedic diseases that can cause activity disorders and complications. Bone marrow mesenchymal stem cells (BMSCs) have osteogenic differentiation and are important seed cells in bone tissue engineering. However, the role of BMSCs in fracture healing remains unclear. SD rats traumatic femoral fracture models were established and were randomly divided into fracture group and BMSCs group, in which BMSCs injected through tail vein followed by analysis of bone mineral density, ALP activity, expression of Runx2, OC and formation of type I collagen by real time PCR, BMSCs migration by Transwell and CXCR4 expression by Western blot. BMSCs can significantly promote the increase of bone mineral density in osteophytes of rats with fracture, increase the expression of Runx2 and OC, promote the increase of ALP activity, increase the expression of type I collagen, the migration ability of BMSCs, as well as significantly increase the expression of CXCR4 compared to fracture group (P < 0.05). BMSCs can increase CXCR4 expression in rats with traumatic fracture. Endogenous BMSCs infusion can promote the migration of BMSCs in vitro, increase the osteogenic transformation of bone defects and promote fracture healing.