Object-based Image Analysis Framework Research Articles

CognitionMaster: an object-based image analysis framework

BackgroundAutomated image analysis methods are becoming more and more important to extract and quantify image features in microscopy-based biomedical studies and several commercial or open-source tools are available. However, most of the approaches rely on pixel-wise operations, a concept that has limitations when high-level object features and relationships between objects are studied and if user-interactivity on the object-level is desired.ResultsIn this paper we present an open-source software that facilitates the analysis of content features and object relationships by using objects as basic processing unit instead of individual pixels. Our approach enables also users without programming knowledge to compose “analysis pipelines“ that exploit the object-level approach. We demonstrate the design and use of example pipelines for the immunohistochemistry-based cell proliferation quantification in breast cancer and two-photon fluorescence microscopy data about bone-osteoclast interaction, which underline the advantages of the object-based concept.ConclusionsWe introduce an open source software system that offers object-based image analysis. The object-based concept allows for a straight-forward development of object-related interactive or fully automated image analysis solutions. The presented software may therefore serve as a basis for various applications in the field of digital image analysis.

Remote Sensing of Ecological Hotspots: Producing Value-added Information from Multiple Data Sources

Fusing high-spatial resolution panchromatic and high-spectral resolution multispectral images with complementary characteristics provides basis for complex land-use and land-cover type classifications. In this research, we investigated how well different pan sharpening algorithms perform when applied to single-sensor single-date and multi-senor multi–date images that encompass the Horton Plains national park (HPNP), a highly fragile eco-region that has been experiencing severe canopy depletion since 1970s, in Sri Lanka. Our aim was to deliver resolution-enhanced multitemporal images from multiple earth observation (EO) data sources in support of long-term dieback monitoring in the HPNP. We selected six candidate fusion algorithms: Brovey transform, Ehlers fusion algorithm, high-pass filter (HPF) fusion algorithm, modified intensity-hue-saturation (MIHS) fusion algorithm, principal component analysis (PCA) fusion algorithm, and the wavelet-PCA fusion algorithm. These algorithms were applied to eight different aerial and satellite images taken over the HPNP during last five decades. Fused images were assessed for spectral and spatial fidelity using fifteen quantitative quality indicators and visual inspection methods. Spectral quality metrics include correlation coefficient, root-mean-square-error (RMSE), relative difference to mean, relative difference to standard deviation, spectral discrepancy, deviation index, peak signal-to-noise ratio index, entropy, mean structural similarity index, spectral angle mapper, and relative dimensionless global error in synthesis. The spatial integrity of fused images was assessed using Canny edge correspondence, high-pass correlation coefficient, RMSE of Sobel-filtered edge images, and Fast Fourier Transform correlation. The Wavelet-PCA algorithm exhibited the worst spatial improvement while the Ehlers.MIHS and PCA fusion algorithms showed mediocre results. With respect to our multidimensional quality assessment,the HPF emerged as the best performing algorithm for single-sensor single-date and multi-sensor multi-date data fusion.We further examined the effect of fusion in the object-based image analysis framework. Our subjective analysis showed the improvement of image object candidates when panchromatic images’ high-frequency information is injected to low resolution multispectral images.

Object-Based Image Analysis of Downed Logs in Disturbed Forested Landscapes Using Lidar

Downed logs on the forest floor provide habitat for species, fuel for forest fires, and function as a key component of forest nutrient cycling and carbon storage. Ground-based field surveying is a conventional method for mapping and characterizing downed logs but is limited. In addition, optical remote sensing methods have not been able to map these ground targets due to the lack of optical sensor penetrability into the forest canopy and limited sensor spectral and spatial resolutions. Lidar (light detection and ranging) sensors have become a more viable and common data source in forest science for detailed mapping of forest structure. This study evaluates the utility of discrete, multiple return airborne lidar-derived data for image object segmentation and classification of downed logs in a disturbed forested landscape and the efficiency of rule-based object-based image analysis (OBIA) and classification algorithms. Downed log objects were successfully delineated and classified from lidar derived metrics using an OBIA framework. 73% of digitized downed logs were completely or partially classified correctly. Over classification occurred in areas with large numbers of logs clustered in close proximity to one another and in areas with vegetation and tree canopy. The OBIA methods were found to be effective but inefficient in terms of automation and analyst’s time in the delineation and classification of downed logs in the lidar data.