Obesity is a condition characterized by the excessive accumulation of body fat beyond normal limits. Irisin and IL-6 are myokines that have a function to convert white adipose tissue into brown adipose tissue, resulting in thermogenesis that induces energy expenditure and has implications for reducing excess fat accumulation. This study aims to demonstrate the response of moderate-intensity treadmill and ergo cycle exercise on fat loss and increasing myokines secretion in obese adolescent females. A total of 30 obese women met the criteria with body mass index (BMI) of 30.02±2.97 kg/m2, age 21.27±1.31 years were recruited into the study and given single session of aerobic ergo cycle exercise (AEEG) and aerobic treadmill exercise (ATEG) during 45 min. Measurement of myokines, i.e., irisin and IL-6, using Enzyme-Linked Immunosorbent Assay (ELISA) on all samples. Statistical analysis was performed using a one-way ANOVA test and Tukey's HSD post hoc test with significance at the 5% level. The results showed that the average levels of irisin post-exercise were 72.82±42.96 ng/mL at CTLG, 282.50±75.96 ng/mL at AEEG, 488.14±61.30 ng/mL at ATEG, and p = 0.000. Average levels of IL-6 post-exercise were 51.09±15.68 pg/mL at CTLG, 58.94±3.62 pg/mL at AEEG, 129.29±52.65 pg/mL at ATEG, and p = 0.000. Average ∆ FAT were -0.02±0.34 % at CTLG, -0.35±0.19 % at AEEG, -0.46±0.18 pg/mL at ATEG, and p = 0.002. Both intervention of exercise consistently increased irisin levels, while IL-6 levels were found to increase only with aerobic treadmill exercise. Likewise, body fat only decreased after one session of treadmill aerobic exercise compared to ergo cycle aerobic exercise. Keywords: Aerobic exercise, interleukin 6, irisin, obesity, metabolism
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