Oak Forests Research Articles

Physicochemical, nutritional properties, and antioxidant potential of ‘limilla’ fruit (Rhus aromatica var. schmidelioides (Schltdl.) Engl.)

Rhus aromatica inhabits humid oak and oakpine forests in the State of Michoacán (Mexico). The fruit of R. aromatica is edible and is traditionally used in the preparation of soft drinks, ice pops, ice creams and ‘atole’. The objective of the present investigation was to carry out a physical and chemical characterization and analysis of the antioxidant capacity of fruit. For the physical characterization, the equatorial and longitudinal diameter, weight and percentage of pulp were determined. In the chemical characterization, a proximal analysis was carried out, quantification of polyphenols and flavonoids was performed, and the antioxidant capacity was determined. The results showed that the fruit had a longitudinal diameter of 6.58 ± 1.02 mm, an equatorial diameter of 7.17 ± 0.66, a weight of 55.22 ± 5.47 mg, and a 40 % pulp proportion. The chemical characterization analysis indicated 8.7 % moisture, 30.6 % lipids, 8.7 % proteins, 29.4 % total sugars, 3.8 % ashes and 18.7 % crude fibre, 3.1 °Brix, pH 3.1, 1.92 % acidity total and a caloric intake of 4.27 kcal/g. The polyphenol content was higher in 60 % ethanol extracts with 88.6 ± 50.89 mg EAG/g; for flavonoids from extracts with 100 % acetone, it was 26.52 ± 0.65 mg EQ/g, and the total carotenoid content was 46.37 mg/100 g. The total antioxidant activity was higher in extracts with 80 % acetone, with 87.17 % inhibition of the DPPH radical and 90 % inhibition of ABTS without showing a significant difference with the different solvents used. The lowest IC50 values were presented in 100 % ethanol and 60 % methanol extracts for the DPPH radical and for the ABTS radical were the 80 % ethanol and 60 % methanol extracts. The lipid, protein, carotenoid, and polyphenol contents and antioxidant capacity of the fruit of R. aromatica were as high as those of other fruits consumed in the human diet.

A Vértesalja homoki cseres-tölgyesei (Fraxino orno-Quercetum cerridis Kevey 2008)

Here we evaluate the phytosociological characteristics of the turkey oak forests on sand (Fraxino orno-Quercetum cerridis) found at the northern foothills of the Vértes Hills, Hungary. Using the traditional Braun-Blanquet method, 20 vegetation samples were collected and compared with turkey oak forests growing in similar ecological situations in other parts of the country. The traditional and multivariate analyses showed that our samples were quite different from the compared forests, and only the forests in the Bakonyalja and the Pilis Mts showed some similarity with the compared forests. Our samples had the lowest proportion of Quercetea pubescentis-petraeae species among the compared forests, and the highest proportion of specialists (Corydalis pumila, Daphne laureola, Doronicum hungaricum, Lychnis coronaria, Physocaulis nodosus, Primula veris, Scutellaria columnae, Sorbus domestica and Veratrum nigrum). The samples were well separated from the compared forests and formed a distinct group.

The nature of vegetation cover and small mammal community structure (on the example of Nizhny Novgorod)

The spatial distribution of Apodemus species (yellow-necked, field, and small forest mice) was studied on the example of the protected area «Oak forest of National Research Lobachevsky State University of Nizhny Novgorod Botanical Garden». Twelve plant associations with non-moral character and representing derivatives of the indigenous type of vegetation were studied. Environmental conditions assessed by the Ellenberg phytoindication method affect the distribution of small mammals in space indirectly. The nature of vegetation cover is more significant. When projective cover increases, the abundance of both small woodland and field mice increases. The strategy of Apodemus agrarius appears to be similar to that of Apodemus uralensis, a species that shows stenobiontic traits in the studied PA. At the same time, insignificant anthropogenic load, a marker of which is the presence of Urtica dioica ruderal species, leads to an increase in the mosaic of vegetation cover, which has a positive effect on the abundance of these small mammal species. A moderate increase in the Berger-Parker dominance index for vegetation cover is positively associated with the confinement of micromammals. In addition, in the conditions of the recreational zone of the upland part of N. Novgorod, Apodemus agrarius prefers areas of old-growth broadleaved forest, not showing the features of anthropophily.

Unraveling habitat-driven shifts in alpha, beta, and gamma diversity of hummingbirds and their floral resource.

Biodiversity, crucial for understanding ecosystems, encompasses species richness, composition, and distribution. Ecological and environmental factors, such as habitat type, resource availability, and climate conditions, play pivotal roles in shaping species diversity within and among communities, categorized into alpha (within habitat), beta (between habitats), and gamma (total regional) diversity. Hummingbird communities are influenced by habitat, elevation, and seasonality, making them an ideal system for studying these diversities, shedding light on mutualistic community dynamics and conservation strategies. Over a year-long period, monthly surveys were conducted to record hummingbird species and their visited flowering plants across four habitat types (oak forest, juniper forest, pine forest, and xerophytic shrubland) in Tlaxcala, Mexico. Three locations per habitat type were selected based on conservation status and distance from urban areas. True diversity measures were used to assess alpha, beta, and gamma diversity of hummingbirds and their floral resources. Environmental factors such as altitude and bioclimatic variables were explored for their influence on beta diversity. For flowering plants, gamma diversity encompassed 34 species, with oak forests exhibiting the highest richness, while xerophytic shrublands had the highest alpha diversity. In contrast, for hummingbirds, 11 species comprised the gamma diversity, with xerophytic shrublands having the highest richness and alpha diversity. Our data reveal high heterogeneity in species abundance among habitats. Notably, certain floral resources like Loeselia mexicana and Bouvardia ternifolia emerge as key species in multiple habitats, while hummingbirds such as Basilinna leucotis, Selasphorus platycercus, and Calothorax lucifer exhibit varying levels of abundance and habitat preferences. Beta diversity analyses unveil habitat-specific patterns, with species turnover predominantly driving dissimilarity in composition. Moreover, our study explores the relationships between these diversity components and environmental factors such as altitude and climate variables. Climate variables, in particular, emerge as significant contributors to dissimilarity in floral resource and hummingbird communities, highlighting the influence of environmental conditions on species distribution. Our results shed light on the complex dynamics of hummingbird-flower mutualistic communities within diverse habitats and underscore the importance of understanding how habitat-driven shifts impact alpha, beta, and gamma diversity. Such insights are crucial for conservation strategies aimed at preserving the delicate ecological relationships that underpin biodiversity in these communities.

After-sealing life in urban soils: Experimental evidence of resilience and efficiency of ectomycorrhizal inoculation

The restoration of urban sealed soils is a major stake for urban planners. In particular, characterizing their resilience from a biotic perspective is of primary importance for remobilizing these artificialized substrates in urban green spaces. Using Marseille (France) as a case study, we implemented a metabarcoding next generation sequencing approach to characterize the diversity and the composition of ectomycorrhizal (EM) fungal communities present in soils sealed for 60 years. Based on an inoculation experiment, we tested the ability of the remaining spore bank to establish EM interactions with plants. Altogether, we aimed at assessing the potential of peri-urban natural habitats to provide an efficient soil EM inoculum for the restoration of urban soils. We found that: (i) long-term sealing drastically reduced the diversity of EM fungi in urban soils, (ii) long-leaved spores of hypogeous gastroid fungi were efficient to establish EM symbioses with plants, and (iii) the efficiency of EM fungal inoculation depended on the natural habitat used as an inoculum source. Oak forests provided species-rich but poorly efficient inoculum. Contrastingly, soils collected in disturbed shrublands (garrigues) hosted moderately-rich EM communities containing droughtadapted species with high ability to colonize urban soils. Our findings highlight that peri-urban landscapes are promising candidates to feed local systems of urban soil restoration. In this perspective, poorly-considered habitats such as shrublands can be considered as valuable sources of soil biota to prevent the over-exploitation of agricultural areas.

Fire protection priorities in the oak forests of Iran with an emphasis on vertebrate habitat preservation

This study examines the impact of fire incidents on wildlife and habitats in the western oak forests of Iran (Zagros region). These forests are globally recognized for their exceptional biodiversity but are frequently threatened by wildfires. To achieve this, the study uses the space–time scan statistics permutation (STSSP) model to identify areas with a higher frequency of fires. The study also analyzes the effects of fires on the Zagros forests from 2000 to 2021 using remote-sensing MODIS data. Also, to understand the elements at risk of fire, burned areas were assessed based on the richness of vertebrate species, determined by the distribution of 88 vertebrate species. The results show that the annual fire rate in the Zagros forests is 76.2 (fire occurrences per year), calculated using the Poisson distribution. Findings show the highest fire rates are found in the northwest and a part of the south of the Zagros. The northwest of the Zagros also has the largest number of single fires and clusters, indicating a wide spatial distribution of fire in these regions. On the other side, it was unexpectedly found that these regions have the richest number of species and higher habitat value. The results demonstrate a significant correlation between the value of the habitat and the extent of burned areas (p < 0.05). The study also reveals that the greatest impact of fires is on small vertebrates. The overlap of frequent fire spots with the richest regions of Zagros oak forests in terms of vertebrate diversity emphasizes the need for strategic forest risk reduction planning, especially in these priority zones.

The Effects of Wild Boar Rooting on Epigeic Arthropods in Oak Forests

The Effects of Wild Boar Rooting on Epigeic Arthropods in Oak Forests

CONDITIONS OF THE OAK FORESTS GROWTH AND CULTIVATION ON THE TOP SURFACES AND SLOPES OF THE NEAR-VOLGA AND ERGENI UPLANDS AND THE PLAINS OF THE SAL-MANYCH INTERFLUVE

Planted oak forests in the south of European Russia are of great conservation and economic importance. The widespread practice of their creation in dry steppes and semideserts dates back to the 1950-1970s and allows to form oak (Quercus robur L.) plantations with longevity up to 40-60 years. The aim of the study is to investigate the growth features of oak trees under different soil-climatic and biocenotic conditions and to suggest methods to increase their longevity.The research was carried out in 2011-2016 in the south of the Near-Volga Upland, the northern part of the Ergeni Upland and lowland plains of the Sal-Manych interfluve at 28 temporary sample plots using common methods of forest inventory and regression data analysis.It has been established that the viability of oak is limited by unfavorable topographic position of plantations, increased climate dryness (HC 0,4-0,6), high content of soil carbonates and shallow-lying horizon of their accumulation, as well as the presence of shrub layer and other competitors for moisture. Under these conditions, afforestation of depressions on the plains and shady slopes with pure plantations of oak and other species without shrubs should gain advantage. The methods of their creation should be modified depending on the topographic position of particular forest area and the climate dryness.So, the main soil cultivation in depressions could last 1-2 years, applying the system of black fallow with plantation plowing. It is advisable to keep 3,5-4,0-meter row spacing, frequent tending, and intensive clearing of young tree stands. Maturer plantations need careful sanitary-selective cuttings, mainly the low-type ones, and preservation of high canopy density. Poor water availability and deterioration of plantations should be prevented by construction of dams.Reclamation plantations of low marketability are created on flat areas after 2-3 years of deeper basic tillage. The carbonate horizon should be loosened and a large moisture reserve is needed. Row spacing of 2,5-3,0 meters is reasonable, accelerating the canopy closure. The area of tree nutrition is expanded by low-intensive uniformly selective cuttings, forming more open stands. The longevity of oak in already existing plantations could be increased by eliminating its competitors, such as living ground cover, shrubs and associated species.

Recover of Soil Microbial Community Functions in Beech and Turkey Oak Forests After Coppicing Interventions

Forest management influences the occurrence of tree species, the organic matter input to the soil decomposer system, and hence, it can alter soil microbial community and key ecosystem functions it performs. In this study, we compared the potential effect of different forest management, coppice and high forest, on soil microbial functional diversity, enzyme activities and chemical-physical soil properties in two forests, turkey oak and beech, during summer and autumn. We hypothesized that coppicing influences soil microbial functional diversity with an overall decrease. Contrary to our hypothesis, in summer, the functional diversity of soil microbial community was higher in both coppice forests, suggesting a resilience response of the microbial communities in the soil after tree cutting, which occurred 15–20 years ago. In beech forest under coppice management, a higher content of soil organic matter (but also of soil recalcitrant and stable organic carbon) compared to high forest can explain the higher soil microbial functional diversity and metabolic activity. In turkey oak forest, although differences in functional diversity of soil microbial community between management were observed, for the other investigated parameters, the differences were mainly linked to seasonality. The findings highlight that the soil organic matter preservation depends on the type of forest, but the soil microbial community was able to recover after about 15 years from coppice intervention in both forest ecosystems. Thus, the type of management implemented in these forest ecosystems, not negatively affecting soil organic matter pool, preserving microbial community and potentially soil ecological functions, is sustainable in a scenario of climate change.

Climate Change-Induced Growth Decline in Planted Forests of Quercus variabilis Blume near Beijing, China

A progressive decline in tree growth may be induced by global warming, affecting tree health and eventually resulting in death, particularly for trees growing in rocky mountainous regions where seasonal droughts have become more pronounced. However, tree growth dynamics in areas experiencing pronounced climate change have received little attention. In this study, a total of 100 (10 m × 10 m) grid plots were investigated in planted forests of Chinese cork oak (Quercus variabilis Blume), which were established in the 1960s and 1970s in a rocky mountainous area near Beijing, northern China. Furthermore, the radial growth of Q. variabilis (a total of 843 trees sampled from the dominant [D], co-dominant [CD], and suppressed [S] crown classes) was analyzed using retrospective dendrochronology and generalized additive models. The effects of meteorological changes between 1962 and 2020 on radial growth across the three crown classes were examined using correlation analysis. The results indicated that the growth of Q. variabilis initially increased and then decreased after 2010 at the regional level, and these trends varied according to crown class. The radial growth of the D trees responded more positively to an increase in temperature and drought severity index (&lt;0 for dry and &gt;0 for wet conditions) compared with that of the CD and S trees. The growth of the D and CD trees continuously increased under higher temperatures during the rainy seasons (June to September); however, the increases were higher for D than for CD trees. In contrast, the radial growth of S trees declined. We confirmed the historical effects of rising temperatures on tree growth and health, which are linked to water availability. Our data suggested that Q. variabilis trees will be considerably affected by intensified droughts. This study furthers our knowledge regarding the impact of climate change on tree and forest growth and provides management strategies for afforestation projects in rocky mountainous areas that are facing climate change.

Contributions of soil organic carbon-induced root- and soil properties complexity to water flow in eastern China

Water flow within the soils affects the efficiency of materials transfer mediated by water. Soil organic carbon (SOC) as an important role in active water flow events can drive the complexity of root-soil synthesis by improving root and soil properties. However, contributions of SOC-induced root- and soil properties complexity to water flow are not well understood. In this study, dye tracing experiments at the three forest stands (oak, pine, and bamboo forests) were conducted to explore water flow patterns, i.e., preferential flow paths (PFP), stream buffer zones (SBZ), and water flow zones (WFZ). X-ray microtomography (CT) scanning was performed to reconstruct the root architecture. The partial least squares path model was applied to quantitatively explore the effects of root- and soil properties on water flow. The results showed that the index of water flow connectivity (IWFC) in the PFP and WFZ patterns decreased with increasing soil depth, while IWFC in the SBZ pattern increased at first and then decreased. In the PFP pattern, soil physical properties had the larger total effects (TE = 0.624) on IWFC change compared with root properties (TE = 0.257). In the SBZ pattern, the total effects of root properties controlling IWFC change (TE = 0.510) were greater than soil physical properties (TE = −0.386). Both of them can equally affect the IWFC in the WFZ pattern. In conclusion, the influences of SOC by driving the changes of soil properties on gravity-driven convective flow process were dramatically stronger than root properties, while SOC could primarily drive the changes of root properties and thereby affect capillary-driven convective flow process. The present results can provide a scientific basis for sustainable forestry management and also a better understanding of the forestry hydrology.

Immediate Impacts of Wildfires on Ground-dwelling macroinvertebrate Communities under Stones in Mediterranean Oak Forests

Wildfires are considered a major disturbance to forest ecosystems in the Mediterranean countries of Southern Europe. Although ground-dwelling macroinvertebrates are crucial to many soil functions, there is a fundamental lack of understanding of how wildfires impact this community in the immediate term and of the role of stones in their survival. Hence, in the present study we assessed the immediate effects of wildfires in the ground-dwelling macroinvertebrate community found under stones by comparing communities in burnt and non-burnt Mediterranean oak forests. Our results revealed that stones allowed the survival of many taxa in the burnt area. However, abundance, richness, diversity, and equitability per stone were significantly lower at the burnt than unburnt sites. Furthermore, the results also showed that richness and abundance increased significantly with increasing stone depth and area, both at the burnt and unburnt sites. Significant changes at the trophic level were observed in the burnt area comparing to the unburnt, particularly a decline in predators. No significant differences were identified concerning habitat associations among taxa. Overall, this study stressed the role of stones as microhabitats and refuge for the ground-dwelling macroinvertebrate community during wildfires.

Volatile Organic Compounds (VOCs) in Mediterranean Oak Forests of Hungarian Oak (Quercus frainetto Ten) Affected by Dieback Phenomena

In recent years, long periods of drought and heat waves have become increasingly frequent, causing forest dieback phenomena that make stands more sensitive to biotic stressors. How trees may respond to extreme climatic events and which metabolites are involved under stress conditions is still not clear. In this study, using Solid Phase Micro-Extraction (SPME)-GC/MS, we analysed how dieback (D) and non-dieback (ND) Hungarian oak trees from the San Paolo Albanese site respond to these climatic dynamics, focusing on volatile organic compounds (VOCs). For each group of trees, three wood samples were taken, and each was divided into four sub-samples with five growth rings and subjected to SPME and increase in basal area (BAI) analysis of the last 20 years. Dieback trees had a lower number of leaves, and this condition may translate into less photosynthesis, less organic matter production, and lower reserves of carbohydrates being available for growth. Indeed, D trees showed lower radial increases and a lower content of aldehydes, terpenes, and fatty acids than ND trees, indicating a better health of ND trees compared to D trees. Meanwhile, D trees showed a reduction in terpenes, such as α-pinene, γ-eudesmol, and cyperene (with significant insecticidal activity), a reduction in aromatic aldehydes, such as furfural and 5-methylfurfural, and an increase in silanols (with antimicrobial function). Considering the different compounds’ contents between D and ND trees, our study could be useful for detecting bio-indicators to identify an early warning signal of dieback phenomena.

Seasonal variations of leaf ecophysiological traits and strategies of co-occurring evergreen and deciduous trees in white oak forest in the central Himalaya.

The present study investigates the seasonal variations in leaf ecophysiological traits and strategies employed by co-occurring evergreen and deciduous tree species within a white oak forest (Quercus leucotrichophora A. Camus) ecosystem in the central Himalaya. Seasonal variations in physiological, morphological, and chemical traits were observed from leaf initiation until senescence in co-occurring deciduous and evergreen tree species. We compared various parameters, including net photosynthetic capacity (Aarea and Amass), leaf stomatal conductance (gswarea and gswmass), transpiration rate (Earea and Emass), specific leaf area (SLA), mid-day water potential (Ψmd), leaf nitrogen (N) and phosphorus (P) concentration, leaf total chlorophyll concentration, photosynthetic nitrogen- and phosphorus-use efficiency (PNUE and PPUE), and water use efficiency (WUE) across four evergreen and four deciduous tree species. Our findings reveal that evergreen and deciduous trees exhibit divergent strategies in coping with seasonal changes, which are crucial for their survival and growth. Deciduous trees consistently exhibited significantly higher photosynthetic rates, transpiration rates, mass-based N and P concentrations (Nmass and Pmass), mass-based chlorophyll concentration (Chlmass), SLA, and leaf Ψmd, while maintaining lower leaf structural investments throughout the year compared to evergreen trees. These findings indicate that deciduous trees achieve greater assimilation rates per unit mass and higher nutrient-use efficiency. Physiological, morphological, and leaf N and P concentrations were higher in the summer (fully expanded leaf) than in the fall (senesced leaf). These insights provide valuable contributions to our understanding of tree species coexistence and their ecological roles in temperate forest ecosystems, with implications for forest management and conservation in the Himalayan region.

Natural regeneration of cork oak forests under climate change: a case study in Portugal

The sustainability of forest species is directly related to the success of stand regeneration. Assuring success is particularly critical in stands where perpetuity relies on natural regeneration, as is often the case with cork oak forests. However, 59% of the stand in Portugal have no natural regeneration, and climate change could further worsen the sustainability of the system. The study summarizes the factors that affect the natural regeneration of cork oak (Quercus suber L.) based on current knowledge and presents a case study on a forest in Northeast Portugal, where the natural regeneration of Quercus suber under the effect of climate change have been monitored and analyzed. The present work focuses on the effect of stand density, i.e., tree cover, on the production of acorns, the establishment and survival of seedlings, and the impact of the summer season on seedling mortality. The monitoring was carried out in February, June, September 2022, and January 2023 in two stands with distinct stand canopy cover, when the region was under extreme drought. Data analysis was performed using the analysis of variance for repeated measures and the Mann–Whitney-Wilcoxon test. The study showed that cork oak regeneration is influenced by stand density, which promoted the establishment success and survival of natural regeneration in a period of reduced precipitation, despite possible competition for water resources. The mean number of seedlings differed significantly between the two stands. However, there were no significant differences in the mean number of seedlings throughout the field measurements. Additionally, the percentage of dead seedlings was low even after the summer season (9.5% of the total seedlings) in the denser stand. These results indicate that high canopy cover can have a protective effect for extreme climatic events and should be considered in forestry management to promote regeneration of the cork oak forests.

Koala density, habitat, conservation, and response to logging in eucalyptus forest; a review and critical evaluation of call monitoring

ABSTRACT This study is the second in a series that examines the habitat requirements and response to logging of koalas (Phascolarctos cinereus) inhabiting tall eucalypt forests of north-east NSW. It presents the findings of koala population and habitat monitoring surveys in Pine Creek State Forest and Bongil Bongil National Park using a combination of call-counting and direct observation (spotlighting). The 6400 ha study area was mapped into 6 zones of increasing koala habitat quality by ground survey of forest structure and floristics on a 200 m grid. The accuracy of habitat definition and mapping was tested by stratified transect counts of koala calls and sightings over two consecutive years (1997–98). Average koala density increased steeply and significantly, from 0.02 – 0.20 koalas/hectare, with increasing mapped habitat quality based on increasing forest age, structural complexity, local food tree species diversity, history of prior koala occurrence and decreased past logging intensity. This relationship was driven primarily by breeding females, with the number of male koala calls weakly or uncorrelated with koala sightings and mapped habitat quality. Male koalas were more widely and uniformly distributed than females, including areas of low quality, plantation, and intensively logged forest. This finding explains the discrepancy between our results and those of other recent studies which concluded that koalas are tolerant of intensive logging based on modelling of calling male koalas and reliance on an untested assumption that male calling is indicative of female breeding success. Koala density in a subset of the highest quality habitat was relatively stable at 0.28 koalas/ha (3 hectares/koala) over the long term (1997–98 and 2012–2023). Key characteristics of the forest koala population, including low stable density, large home ranges, preference for high food tree diversity and locally unique food trees (including Allocasuarina torulosa and Syncarpia glomulifera), are not adequately explained by existing koala habitat models. We present a new paradigm to explain regional variation in koala distribution, habitat and foraging preferences based on variations in foliage chemistry (toxicity and nutritional value) determined by the duration and stability of local plant-koala interactions in response to past fire, hunting, predation and logging disturbance history. We hypothesize that koala density in stable forest populations is regulated at low levels by a combination of selected and induced increases in leaf toxicity and decreases in leaf nutrition that limit koala browsing to benign levels of about 1-2% of annual leaf production. Large home ranges, complex mature forest structure, high food tree diversity and a specialized or diverse gut microbiome may be essential to allow females to rotate and change food trees frequently to minimize induced toxicity and select individual leaves with sufficient nutrients to support breeding and lactation with minimal risk of predation. High density koala populations (&amp;gt; 0.6/ha) occur primarily in areas where koalas have been introduced or re-introduced to planted habitats and natural areas where aboriginal hunters and dingoes were historically present but are now absent, and where food trees have not been selected for resistance to koala browsing pressure.

Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests.

Soil bacterial communities play a remarkable role in nutrient cycling, significantly affecting soil organic material content, soil fertility, and, in an indirect way, plant succession processes. Conversely, vegetation type influences microbial soil life. The present study compared the bacterial microbiome composition, diversity and catabolic activity profile of topsoil samples collected under three different forest types (a twice-coppiced black locust stand, a young, naturally reforested, and a middle-aged mixed pedunculate oak stand) planted on former arable land in the early 20th century. Diversity indices determined during 16S ribosomal RNA sequencing-based metagenome analysis indicated that the black locust stand had the highest soil bacterial community diversity. At the phylum level, Acidobacteriota, Actinobacteriota, Proteobacteria, Verrucomicrobiota, Bacteroidota, and Gemmatimonadota were the most abundant taxa in the forest soils. Concerning soil parameters, redundancy analysis revealed that pH had the highest impact on bacterial community structure and pH, and soil organic carbon content on the samples' respiration patterns. As for catabolic activity, the recently clearcut oak forest showed the lowest substrate-induced respiration, and citrate was the main driver for the inter-stand variability of microbial activity. Our results confirm that soil parameters and forest type influence the composition and functioning of the soil bacterial microbiome.

Insights into haplotype distribution and population dynamics of Biscogniauxia mediterranea in cork oak forests in Tunisia

Biscogniauxia mediterranea is a pathogenic fungus associated with Quercus species causing charcoal canker disease in forests. Its phenotypic and genetic variability has been well studied in Tunisia but little is known about its demographic history. Population dynamic of B. mediterranea was evaluated among 30 isolates from different cork oak forests in Northwestern Tunisia. The genetic structure analyzed with two DNA markers (ITS and tef-1α) showed low genetic differentiation associated with high genetic flow among populations. The polymorphism analysis showed low nucleotide diversity but high haplotypic diversity among and within B. mediterranea populations which could be due to rapid growth from a small ancestral population. This hints at recent population growth after a period of decline likely due to a catastrophic event involving fires and human-induced tree cutting. The resulting genetic diversity within populations primarily arises from the emergence of new haplotypes. Overall, this study provides insights into the polymorphism of B. mediterranea and its potential adaptive strategy.

Square-grid sampling to address the vegetation patterns of declined Mediterranean forest ecosystems

Palo Laziale Wood is a relatively small biotope (129 ha) situated along the coast of the Metropolitan Area of Rome, Lazio region, Italy. Despite being one of the region's remaining patches of an ancient oak floodplain broad-leaved forest, it conserves numerous priority habitats and species of high conservation interest. The vegetation consists mainly of Turkey oak stands with small temporary ponds and flooded meadows. The forest underwent a dieback in 2003, triggered by a significantly hot and dry summer. In 2018, an ecological restoration project (LIFE PRIMED LIFE17 NAT/GR/000511) was initiated to restore Palo Laziale’s ecosystems. This paper presents the methodological approach employed to assess the vegetation ecology of a degraded forest ecosystem. Such an investigation was a key component of the Adaptative Restoration Plan of the Project. It provided the baseline necessary for designing and calibrating the planned direct conservation actions on the target habitat types (91M0: Pannonian-Balkanic turkey oak-sessile oak forests, 3170*: Mediterranean temporary ponds, 5230*: Arborescent matorral with Laurus nobilis, etc.) and establishing reference values to enable long-term monitoring. Plant taxa comparison from 1 ha square-grid sampling and multivariate analyses were carried out to group species and identify environmental and Ellenberg-based drivers. Six ecologically distinct units were found, eventually confirming the distinctive ecological heterogeneity of Mediterranean ecosystems. Amongst these, the hygrophilous vegetation has resulted to be the one mainly affected by the dieback outbreak. Due to the high heterogeneity, introduced by the massive tree mortality, the method of regular 1 ha squares turned out to be a reliable alternative to random vegetation sampling plots (e.g., phytosociological relevés) to disentangle ecological patterns of fragmented and disturbed habitats.

Catalogue of Palamocladium euchloron (Müll. Hal.) Wijk et Margad. (Hypnales, Brachytheciaceae) specimens deposited in the State Museum of Natural History NASU, Lviv, Ukraine

The species Palamocladium euchloron (Müll. Hal.) Wijk et Margad. is distributed in Southern Europe (Greece), in the Caucasus, in Asia (Turkey, Iran, Turkmenistan, Pakistan, China). Findings in the Mountainous Crimea: Kirovskyi district, Agarmysh Mt. in the vicinity of the Old Crimea town, Old Crimea; Khapkhal Gorge near the Dzhur-Dzhur waterfall on the East Ulu-Uzen River, Crimean Nature Reserve, upper reaches of the Alma River; Bakhchysaraiskyi district, near the Bakhchysarai town, Sokolyne and Mashyne villages, Chufut-Kale rock . The species is scientifically significant as a relict (tertiary) one. This moss grows on the soil, on shaded limestone rocks and stones in oak and beech forests. The plant is dioecious. In the Crimea it does not form sporogonies and reproduces vegetatively. Palamocladium euchloron (Müll. Hal.) Wijk et Margad. is protected in the Crimean Nature Reserve, Khapkhalskyi hydrological reserve and the Agarmyskyi Forest landmark of nature . The species is recorded for the Staryi Krym vicinity in the work of M.P. Slobodian . The species is given in the study of fossil Pliocene bryoflora for the territory of Poland . Specimens of the Palamocladium euchloron (Müll. Hal.) Wijk et Margad., represented in the SMNH collection, were collected from the territory of the Crimean Peninsular (Bakhchysarai (Slobodian, 1969), Kirovsky district (Slobodian, 1966), Sudak town (Slobodian, 1968), Sokolyne village (Slobodian, 1969), Mashyne village (Slobodian, 1969), Golovkinskyi waterfall (Melnychuk, 1954); from the territory of Krasnodar Krai (Russia) territory were collected in Krasnaia Poliana (Lazarenko, 1937), from the territory of Azerbaijan near Novo-Alekseevka village (Окснер, 1936). The museum collection contains 14 specimens of Palamocladium euchloron (Müll. Hal.) Wijk et Margad. Two specimens are exsiccates (Institutum Botanicum nomine V.L. Komarivii Academiae Scientiarum URSS, 1961). Samples were collected by M.P. Slobodian, V.M. Melnychuk, A.S. Lazarenko, A.M. Oksner. Exsiccates were collected by I.I. Abramov and A.L. Abramova. Most specimens were collected in the second half of the 20th century. The specimens of A.S. Lazarenko and A.M. Oksner belong to the first half of the 20th century. The species has been included into the Red Data Book of Ukraine with conservation status: Rare. This article was written with the support of the National Research Foundation of Ukraine, call 2022.01 «Science for the Recovery of Ukraine in the War and Post-War Periods» the project 2022.01/0013 «Digitization of natural history collections damaged as a result of hostilities and related factors: development of protocols and implementation on the basis of the State Museum of Natural History of the National Academy of Sciences of Ukraine». The contents of this article do not necessarily reflect the views of the National Research Foundation of Ukraine and are the sole responsibility of the State Museum of Natural History, NAS of Ukraine.