Nutritional Resources Research Articles

Linking summer nutrition to behavior and performance of black‐tailed deer

AbstractMany large‐herbivore populations are regulated at least in part by bottom‐up forces, and thus relationships between herbivores and their habitat are of fundamental importance to wildlife managers. Variation in nutritional resources—and how herbivores respond to that variation—influences rates of nutrient intake, which directly affect nutritional condition, pregnancy rates, timing of parturition, offspring birth mass, and survival. Accordingly, nutrition‐focused research holds great potential for uncovering the mechanisms that govern population performance of large herbivores and for assessing the nature and magnitude of bottom‐up limitations. We quantified relationships between the foodscape (i.e., spatiotemporal variation in forage quality and abundance) in southwestern Oregon, USA, and black‐tailed deer (Odocoileus hemionus columbianus) behavior and performance, with a focus on the influence of maternal nutrition on fawn survival. We hypothesized that black‐tailed deer performance (i.e., fawn birth mass and survival) is influenced by the availability of high‐quality forage during spring and summer and patterns of foodscape use exhibited by individual deer. From 2016–2023 we monitored movement and survival of adult female black‐tailed deer and their offspring. We also conducted intensive vegetation sampling and used generalized additive modeling to map the foodscape available to deer in spring and summer. Suitable forage biomass (i.e., maximum biomass of forage that together exceeded quality thresholds for supporting one fawn) was highly variable across space and time, and our top foodscape model explained 70% of the variation in suitable biomass (adjusted R2 = 0.70). We observed a strong, positive relationship between use of the foodscape by maternal females prior to parturition and fawn birth mass. Although maternal foodscape use after parturition did not influence the probability of fawn survival, survival increased with increasing birth mass. These results suggest that the effects of nutrition on fawn survival in our study system are indirectly mediated by maternal behavior (i.e., use of the foodscape) and the corresponding effects on birth mass of fawns. Our study adds to a growing body of literature supporting a fundamental link between foodscape use and population performance of large herbivores. Wildlife managers can use the dynamic models we developed to assess habitat quality and to make quantitative predictions about how different management actions (e.g., forest thinning) are likely to influence habitat quality and performance of black‐tailed deer.

Socially plastic responses in females are robust to evolutionary manipulations of adult sex ratio and adult nutrition.

Socially plastic behaviours are widespread among animals and can have a significant impact on fitness. Here, we investigated whether the socially plastic responses of female Drosophila melanogaster can evolve in predictable ways following long-term manipulation of adult sex ratio and adult nutrient availability. Previous reports show that female D. melanogaster respond plastically to their same-sex social environment and lay significantly fewer eggs after mating when previously exposed to other females. In this study, we tested 2 hypotheses, using females drawn from lines with an evolutionary history of exposure to variation in adult sex ratio (male-biased, female-biased or equal sex ratio) and adult nutritional environment (high or low quality). The first was that a history of elevated competition in female-biased regimes would select for increased plastic fecundity responses in comparison to females from other lines. The second was that these responses would also be magnified under poor nutritional resource regimes. Neither hypothesis was supported. Instead, we found that plastic fecundity responses were retained in females from all lines and did not differ significantly across any of them. The lack of differences does not appear to be due to insufficient selection, as we did observe significant evolutionary responses in virgin egg-laying patterns according to sex ratio and nutritional regime. The lack of variation in the magnitude of predicted plasticity is consistent with the idea that the costs of maintaining plasticity are low, benefits high, and that plasticity itself can be relatively hard wired.

Microbiota of Attine Ants' Gardens: Visualizing a Microbial Landscape by Scanning Electron Microscopy.

In macroscale ecosystems, such as rainforests or coral reefs, the spatial localization of organisms is the basis of our understanding of community ecology. In the microbial world, likewise, microscale ecosystems are far from a random and homogeneous mixture of organisms and habitats. Accessing the spatial distribution of microbes is fundamental for understanding the functioning and ecology of the microbiota, as cohabiting species are more likely to interact and influence each other's physiology. An interkingdom microbial ecosystem is at the core of fungus-growing ant colonies, which cultivate basidiomycete fungi as a nutritional resource. Attine ants forage for diverse substrates (mostly plant-based), metabolized by the cultivated fungus while forming a spongy structure, a "microbial garden" that acts as an external gut. The garden is an intertwined mesh of fungal hyphae growing by metabolizing the substrate, opening niches for a characteristic and adapted microbiota to establish. The microbiota is thought to be a contributor to substrate degradation and fungal growth, though its spatial organization is yet to be determined. Here, we describe how we employ Scanning Electron Microscopy (SEM) to investigate, with unprecedented detail, the microbiota and biofilm spatial organization across different fungiculture systems of fungus-growing ants. SEM imaging has provided a description of the microbiota spatial structure and organization. SEM revealed that microbiota commonly assemble in biofilms, a widespread structure of the microbial landscapes in fungiculture. We present the protocols employed to fix, dehydrate, dry, sputter coating, and image such a complex community. These protocols were optimized to deal with delicate and heterogeneous samples, comprising plant and fungal biomass, as well as the microbiota and the biofilm.

Strengthening nutrition policy and service delivery: Lessons learned from a six-country assessment of Alive and Thrive's technical assistance.

Alive & Thrive (A&T) provides strategic technical assistance (TA) to develop effective policies; improve maternal, infant, and young child nutrition (MIYCN) programme design and implementationand enhance system capacity to sustain quality MIYCN service delivery at scale. A qualitative assessment was conducted using document review and stakeholder interviews (n = 79) to describe a selection of A&T's TA in six countries and systematically assess the contextual and TA process-related factors that influenced the results achieved and document the lessons learned about MIYCN TA design and implementation. To facilitate the selection of different types of TA, we classified TA into two levels of stakeholder engagement and intensity. Under the Technical Advisor TA category, we assessed A&T's support to strengthen national policy formulation, monitoring, and implementation of the International Code of Marketing of Breast-milk Substitutes. For Capacity Development TA, we assessed A&T support to scale-up maternal nutrition services and to increase strategic use of data. Factors important for TA provision included identifying and engaging with the right people, using evidence to support advocacy and decision-making, using multiple ways to strengthen capacity, developing packages of tools to support programme scale-up, and reinforcing feedback mechanisms to improve service provision and data quality. Challenges included shifts in the political context, poorly functioning health systems, and limited resources to replicate or sustain the progress made. Continued investment in evidence-based and practical TA that strengthens the institutionalization of nutrition across all stakeholders-including government, medical associations, civil societyand development partners-is essential. Future TA must support governments to strengthen system capacity for nutrition, including financial and human resource gaps that hamper full scale-up.

Modified culture media development using locally available fertilizers and vitamin supplements for continuous mass culture of Chaetoceros gracilis

Microalgae culture is a vital nutritional resource for hatchery development in aquaculture and has gained significant interest as a sustainable food ingredient, versatile feedstock for biofuels, with broad industrial applications. The high cost of microalgae culture media hinders mass production, necessitating the development of affordable, simpler alternatives for commercial viability. The aim of this study was to develop a modified culture media for Chaetoceros gracilis utilizing locally available fertilizers and vitamins. In the first experiment, the cell density of C. gracilis across four fertilizers combinations (T1, T2, T3 and T4) with three replicates was evaluated to identify the best performing fertilizers recipe. The significantly higher cell density was observed in T1, where a combination of urea, TSP, mineral mix, EDTA, silicate gel, NPK, compost were used, than the other treatments where one or more ingredients were missing. The growth curve showed that with 1 ml/L fertilizers combination of T1 cell density peaked (21.8×10^5 cell/ml) at day three with the death phase starting at day 04. In the second experiment, the effect of vitamin addition was evaluated with T1 considering as control (V0-without vitamin supplement) and, the Multivitamin-Zinc B and Neobion addition with T1 as treatment V1 and V2, respectively. While Neobion addition significantly increased cell density, multivitamin-Zinc B addition reduced the cell density. Finally, the potentiality of continuous culture of C. gracilis with the modified fertilizer media (V2) was evaluated. The cell density in 300 L tank culture starting from 6.6×10^5 cell/ml peaked 23.8 ×10^5 cell/ml at day two and remained relatively static until investigation finished with the supplement of V2 media two days interval. The present study suggest that development of modified fertilizer media using affordable, locally sourced ingredients for algae cultivation in the hatcheries and aquaculture industry could create an alternative to relying on pure laboratory-grade media.

Lower adaptive immunity in invasive Egyptian geese compared to sympatric native waterfowls

Successful invasive species increase their spreading success by trading-off nutritional and metabolic resources allocated to reproduction and range expansion with other costly body functions. One proposed mechanism for the reallocation of resources is a trade-off with the immune function and the regulation of oxidative status. Relying on a panel of blood-based markers of immune function and oxidative status quantified in an invasive species (Egyptian goose) and two native competing species (mallard and mute swan) in Germany, we tested the hypothesis that the invasive species would have (i) lower investment in immune function, (ii) lower levels of oxidative damage, and (iii) no higher antioxidant defences compared to the native species. We found lower levels of adaptive immune markers (lymphocytes and immunoglobulin Y), in the invasive species compared to the two native species. Innate immune profile was generally similar between Egyptian geese and mallards. By contrast, mute swans showed higher levels of heterophils and lysozymes, and lower levels of bacteria killing ability compared to both Egyptian geese and mallards. Mute swans also showed higher levels of haemolysis and haemagglutination, but lower levels of monocytes and haematocrit compared to Egyptian geese. Reactive oxygen metabolites, a marker of oxidative damage, were higher in mallards and lower in Egyptian geese compared to the other waterfowl species, while levels of antioxidants were generally similar among the three species. Our results point to a reduced investment in adaptive immune function in the invasive species as a possible resources-saving immunological strategy due to the loss of co-evolved parasites in the new colonised habitats, as observed in a previous study. A lower investment in immune function may benefit other energy-demanding activities, such as reproduction, dispersal, and territoriality, while maintaining relatively higher innate immunity is beneficial since invasive species mainly encounter novel pathogens. Results pointed out also other important species-specific differences in baseline immune status, supporting previous findings on the relationship between species' body mass and immune profile.

Healthy from the Start: Co-Designing Sleep, Nutrition and Physical Activity Resources for Young Shiftworkers-Novel Implementation and Evaluation.

The increasing prevalence of shiftwork among young adults poses significant health risks, primarily due to its disruptive effects on sleep, nutrition and physical activity. Addressing these risks necessitates the development of tailored, evidence-based resources to support these key health behaviours. Participatory research approaches, engaging those with relevant lived experience (i.e., co-design) are a novel and effective approach in developing these resources. As such, the aim of the present study was to explore whether sleep, nutrition and physical activity resources for young shiftworkers could be developed using participatory, co-design approaches and how co-designers would rate both the approaches used and the resulting resources. A participatory approach engaged co-designers (young, experienced or previous shiftworkers; workplace health and safety specialists; science communicators and academic experts) to complete 2-3 online questionnaires and participate in 1-2 online workshops, to co-design sleep, nutrition and physical activity resources for young shiftworkers. Following resource development, co-designers assessed both the participatory approach and the resulting resources, through an online questionnaire, which included the Public and Patient Engagement Evaluation Tool (PPEET). Co-designers (n = 48) participated in the development of sleep, nutrition and physical activity resources for young shiftworkers. Co-designers evaluated the participatory approach positively, with a mean rating across all PPEET items of 4.7 (±0.2) on a 5-point Likert scale. Co-designers also provided positive ratings for the resources, with the majority (91.7%) either agreeing or strongly agreeing that they were user-friendly, valuable and informative for young shiftworkers and would serve as a credible source of health information. By adopting a novel participatory approach, we successfully co-designed sleep, nutrition and physical activity resources for young shiftworkers. Participatory approaches, including co-design, should be considered when developing health interventions for shiftworkers, given the value of embedding lived experience to address their unique lifestyle challenges. Co-designers and/or people with relevant lived experience were involved in all project activities: conceptualisation, design, recruitment, data collection, data analysis, knowledge translation and output generation.

Preparing Ganoderma lucidum slices by improved steam explosion enhances their apparent, functional and structural properties

Ganoderma lucidum slices exhibit antioxidant and enzyme inhibitory activity, enhancing vitality and longevity. However, untreated Ganoderma lucidum slices fail to meet specific physical and chemical requirements for consumption and processing because their bioactive compounds must be released before intake through effective pretreatment. We proposed developing a novel preparation method for Ganoderma lucidum slices through improved steam explosion (ISE). The effect of ISE (at 0.4–1.2 MPa for 5 min) on their chemical compounds, apparent properties, biological activity, sensory quality and structural characteristics was assessed. The results showed that ISE (0.8–1.2 MPa) increased the content of flavonoids (by 32.29 %–126.57 %), phenolics (by 31.79 %–96.28 %), triterpenes (by 1.74–5.24 times) and polysaccharides (by 6.27–18.03 times) in Ganoderma lucidum slices. Additionally, ISE improved the color saturation and hue of their infusions while reducing the color values of these slices. Under all conditions, ISE significantly increased the soluble solids content (by 1.31–11.44 times) and the inhibitory activity of tyrosinase and xanthine oxidase when compared to infusions of untreated slices. At 0.8–1.2 MPa, the radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was also significantly higher in steam-exploded slices than in untreated slices. In the sensory evaluation, steam-exploded slices outperformed untreated slices in terms of color, taste, form attributes, and overall score. ISE disrupted the microstructure of Ganoderma lucidum slices, whose microscopic structure was broken into fragments, thereby promoting the dissolution of bioactive compounds and enhancing their biological functions, as shown in this study. Therefore, ISE improved the nutritional and medicinal effects of the fruiting body of Ganoderma lucidum by facilitating nutritional retention and resource utilization efficiency.

Physiological and biochemical attributes of the association host- parasite Tamarix aphylla-Plicosepalus acacia

Parasitic plants have been viewed as pests since they are able to damage agricultural crops and forest trees. They establish vasculature connections with the hosts and withdraw the required nutritive resources. This study aimed to explore the physiological and biochemical effects of the parasitic plant Plicosepalus acacia on the host Tamarix aphylla. It was conducted on young fully expanded leaves from the uninfected and infected trees and the parasitic plant ‘in situ’. The parasite had higher net photosynthetic assimilation rate (A), transpiration rate (E) and stomatal conductance (gs) compared to the host. Equally, it had two-fold greater water potential (Ψ) and osmotic potential (Ψs). It accumulated high amount of K, while it avoided accumulation of the most trace and ultratrace elements. Otherwise, parasitism seemed to increase A, WUE, water uptake and accumulation of the most major, trace and ultra-trace elements, however it reduced the accumulation of osmolytes at the infected plants. Based on UPLC-MS approach, P. acacia seemed to use a group of composites to interact with the host, including oleamide as a protector metabolite against host’s defense system, carvone to establish vasculature connections with the host, cuminaldehyde to weaken growth and proliferation of the host, and caprolactam to weaken the distal part of the host. In contrast, the host T. aphylla could be used pipecolinic acid and nicotinamide to regulate systemic resistance and to defense against the parasite infection. Finally, despite the defense molecular interactions between the two partners, the parasitic plant exhibited several beneficial effects on the host.

Associations between Parenting and Cognitive and Language Abilities at 2 Years of Age Depend on Prenatal Exposure to Disadvantage

To investigate whether parenting or neonatal brain volumes mediate associations between prenatal social disadvantage (SD) and cognitive/language abilities and whether these mechanisms vary by level of disadvantage. Pregnant women were prospectively recruited from obstetric clinics in St Louis, Missouri. Prenatal SD encompassed access to social (eg, education) and material (eg, income-to-needs, health insurance, area deprivation, and nutrition) resources during pregnancy. Neonates underwent brain magnetic resonance imaging. Mother-child dyads (N=202) returned at age 1-year for parenting observations and at age 2-years for cognition/language assessments (Bayley-III). Generalized additive and mediation models tested hypotheses. Greater prenatal SD associated nonlinearly with poorer cognitive/language scores. Associations between parenting and cognition/language were moderated by disadvantage, such that supportive and non-supportive parenting behaviors related only to cognition/language in children with lesser prenatal SD. Parenting mediation effects differed by level of disadvantage: both supportive and non-supportive parenting mediated prenatal SD-cognition/language associations in children with lesser disadvantage, but not in children with greater disadvantage. Prenatal SD-associated reductions in neonatal subcortical grey matter (β=.19, q=.03), white matter (β=.23, q=.02), and total brain volume (β=.18, q=.03) were associated with lower cognition, but did not mediate associations between prenatal SD and cognition. Parenting moderates and mediates associations between prenatal SD and early cognition and language, but only in families with less social disadvantage. These findings, although correlational, suggest that there may be a critical threshold of disadvantage, below which mediating or moderating factors become less effective, highlighting the importance of reducing disadvantage as primary prevention.

Understanding the Potential of Microbial Protein as a more Sustainable Food Source

Abstract The increasing global demand for protein, driven by population growth and dietary shifts, poses a challenge to conventional, inefficient food production systems. In response, alternative protein sources like microbial protein have gained traction for their potential to supply nutritional food more sustainably. Microbial protein, the protein-rich biomass of microorganisms, offers a promising solution due to its high nutritional value, resource efficiency, and climate resilience. However, challenges persist in transitioning to the use of sustainable feedstocks and navigating complex legislative frameworks, hindering widespread adoption. Effective legislative reforms, streamlined regulatory processes, and technological innovations are essential to unlock the full potential of microbial protein as a more sustainable food source. Moreover, consumer acceptance and behavioral changes are crucial for driving adoption and harnessing the environmental benefits of microbial protein. Ultimately, collaborative efforts across stakeholders, informed consumer choices, and policy interventions are imperative for realizing a sustainable food future, with microbial protein playing a pivotal role in this transformative journey. Information © The Authors 2024

Moringa-based homestead to achieve Sustainable Development Goals: A case study from Jaintiapur of Sylhet, Bangladesh

A study was conducted in Sylhet at Jaintiapur Upazila to determine the prospects of Moringa-based homestead concerning Sustainable Development Goals. A household survey was conducted following a simple random sampling of 135 farmers and following a semi-structured questionnaire and interview schedule with 100 farmers (40 identified Moringa-based adopters and 60 non-adopters). The final questionnaire was prepared after pilot testing, which contained data on common species diversity, and the perception of farmers regarding SDGs indicators of “no poverty, zero hunger, good health, and well-being, gender equality, affordable and clean energy, decent work and economic growth”. The extent of agreement was recorded following the points Likert scale high (3) to no change (0), and the SDG index (SDGI) value was calculated. The 10 key informant interviews were conducted with non-adopters to get insights into their perception regarding Moringa-based homesteads. The findings revealed that the status of plant species diversity such as betel nut (100 %), mango (100 %), bean (99 %), and arjun (90 %) was higher in comparison to non-adopters where the status of the respective species was 92 %, 99 %, 89 %, and 73 % respectively. The perception assessment revealed that 100 % of adopters and 90 % of non-adopters believed that Moringa-based homesteads had the potential to increase access to food, nutrition, and medicinal resources. While the majority of 70 % of adopters, and 90.5 % non-adopters disagreed that it had the potential to ensure government access and non-government credit resources. Among the Moringa-based homestead adopters, the gross income derived from Moringa sales was 2828.57 ± 1481.45, where 55 % of homesteads were identified to have Moringa plants between 3 and 5, and homesteads solely supplied fuel materials for 40 % of households. Gender participation was quite evident for homestead farming activities, where female participation was higher in planting, weeding, irrigation, and fencing. Homesteads provided both on-farming and off-farming income opportunities where major responses were found for poultry farming (96 %), vegetable farming (95 %), and day labor (97 %). Farmers were found to practice climate-smart practices of homestead agroforestry (100 %), rainwater harvesting (99 %), weeding (98 %), and management of debris (95 %) in their respective homesteads. The results disclosed that Moringa-based homestead in Sylhet can be a potential option for attaining SDGs indicators of escalation of household income (SDGI = 90), access to food, nutrition, and medicinal resources (SDGI = 103.6), facilitation of natural treatment of diseases (SDGI = 104.6), de-escalation of gender discrepancy in terms of production activities (SDGI = 103.64), own source of fuel (SDGI = 58.44), both off-farm and on-farm income opportunity (SDGI = 100.52), ecosystem health maintenance by resilient practices (SDGI = 104.6). Farmers ranked food security capacity as a major motivational factor, while the low economic return was a major demotivational factor. The escalation of Moringa-based homesteads needs to be prioritized while facilitating credit, and institutional support to extend encouragement to non-adopters for wider integration of Moringa-based homesteads, and appropriate utilization of the existing resources for greater profitability.

Diet and multiple sclerosis – development and mixed methods feasibility testing of a comprehensive nutritional information resource (NUTRIMS)

Purpose Persons with multiple sclerosis (pwMS) are often confused by contradictory dietary advice. The purpose of this research was to explore the information needs and design a comprehensive, evidence-based nutritional information resource on diet and MS (NUTRIMS). Methods A mixed-methods design with three sequential phases was adopted: (1) Needs assessment and development: Two focus groups with pwMS to explore experiences, information needs and preferred support around diet. Development of a draft NUTRIMS, (2) Feasibility Step 1: Feedback on the draft NUTRIMS from stakeholders and (3) Feasibility Step 2: Online survey among pwMS to explore content quality and acceptability. Results Two focus groups with a total of 12 pwMS showed that MS-specific evidence on food groups/ingredients, nutrients and special diets were of most interest. The draft NUTRIMS was refined through feedback from 13 stakeholders. The pre-final NUTRIMS consisted of 81 pages including scientific references on each topic, 16 illustrations and a glossary of terms. 85 pwMS participated in the online survey and reported an intensive use and high satisfaction with the information resource, which was perceived as comprehensible, highly credible and useful for making decisions on dietary change. Conclusions A collaborative research approach led to a well-accepted nutrition information resource.

THE IMPACT OF SOCIOECONOMIC FACTORS ON NUTRITIONAL KNOWLEDGE AND DIETARY PRACTICES AMONG SCHOOL-GOING ADOLESCENTS

Objectives: This study investigates the impact of socioeconomic factors on nutritional knowledge and dietary practices among school-going adolescents in rural areas of Jodhpur, Rajasthan. The primary objectives were to assess the relationship between socioeconomic status and adolescents' nutritional knowledge and dietary practices, and to identify barriers to adopting healthy dietary practices in the context of socioeconomic disparities. Methods: A cross-sectional survey was conducted, encompassing data from 385 adolescents, with variables such as family income, parental education, and access to nutritional resources being analyzed. Results: The results revealed significant correlations between higher socioeconomic status and better nutritional knowledge and dietary practices. Adolescents from wealthier families and those with more educated parents demonstrated superior understanding and adherence to healthy dietary guidelines, while those from lower socioeconomic backgrounds exhibited gaps in knowledge and poorer dietary habits. Additionally, the study highlighted the presence of nutritional health issues, including both underweight and overweight adolescents, indicating a dual burden of malnutrition in the region. Conclusion: The conclusion emphasizes the need for targeted public health interventions that address the socioeconomic disparities influencing adolescent nutrition. Such interventions should include enhanced nutritional education, improved access to affordable nutritious foods, and culturally sensitive programs that respect local dietary practices while promoting balanced nutrition. The findings underscore the critical role of socioeconomic factors in shaping adolescents' nutritional behaviors and call for multi-faceted approaches to improve nutritional outcomes in rural communities. KEYWORDS: Nutrition knowledge, Dietary practices, Adolescent Nutrition, Public Health, Nutritional Education

Transcriptome and metabolome analyses reveal the effects of formula and breast milk on the growth and development of human small intestinal organoids

Breast milk is widely acknowledged as the ideal nutritional resource for infants and can well meet the nutritional requirements for baby’s growth and development. Infant formula is a substitute for breast milk, designed to closely mimic its composition and function for breast milk. Most of the previous studies used tumor colorectal cancer cell lines to study the nutritional potency of formula and its components, so realistic data closer to the baby could not be obtained. Small intestinal organoids, derived from differentiated human embryonic stem cells, can be used to simulate nutrient absorption and metabolism in vitro. In this experiment, we used small intestinal organoids to compare the nutrient absorption and metabolism of three infant formulae for 0–6 months with breast milk samples. Transcriptome and metabolome sequencing methods were used to analyze the differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs). The pathways related to DEGs, DEMs were enriched using GO, KEGG, GSEA and other methods to investigate their biological characteristics. We have found that both formula and breast milk promote the development of the infant’s immune system, nutrient absorption and intestinal development. In PMH1 we found that the addition of oligofructose to milk powder promoted lipid metabolism and absorption. In PMH2 we found that whey protein powder favours the development of the immune system in infants. In PMH3 we found that oligogalactans may act on the brain-gut axis by regulating the intestinal flora, thereby promoting axon formation and neural development. By linking these biological properties of the milk powder with its composition, we confirmed the effects of added ingredients on the growth and development of infants. Also, we demonstrated the validity of small intestine organoids as a model for absorption and digestion in vitro. Through the above analyses, the advantages and disadvantages of the roles of formula and breast milk in the growth and metabolism of infants were also compared.

On the stability of a single-species model with a generic delay distribution kernel

The delayed logistic equation, also known as Hutchinson’s equation, is a simple and elegant model commonly used to capture critical features of complex phenomena in biology, medicine, and economics. This paper studies the stability of a single-species logistic model with a general delay distribution and a constant inflow of nutritional resources. We provide conditions for the linear stability of the positive equilibrium and the occurrence of Hopf bifurcation. The findings complement existing literature and are applied to specific delay distributions: Uniform, Dirac-delta, and gamma distributions. Without resource inflow, we find that the positive equilibrium is stable for short delays but loses stability through Hopf bifurcation as the mean delay increases. The model’s dynamics vary with resource inflow based on the delay distribution: in uniform and Dirac-delta distributions, the dynamics are similar to the no-inflow case, whereas for the gamma distribution, stability depends on the delay order p=1,2,3.

Nutrition and physical activity practices in family day care: A cross-sectional survey of Australian family day care educators.

Munch & Move is a New South Wales (NSW) Ministry of Health program offering family day care (FDC) educators training to support children's healthy behaviours. This study examined educators' nutrition, physical activity and screen time practices and relationships between Munch & Move training and professional development (PD) on these practices. NSW FDC educators (n = 186) completed an online survey from July 2020-June 2021. Differences between groups based on Munch & Move training (trained; not trained) and PD (those who completed PD ≥1 time per year; those who completed PD <1 time per year or never) were tested using bivariate analyses. A significantly higher proportion of educators trained in Munch & Move offered information to families regarding food serving sizes, nutrition policies, and children's physical activity and screen time. Over one-third in both groups did not meet the guideline of no screen time for children under 2 years old. Compared with those who completed PD ≥1 time per year, a significantly higher proportion of educators who completed PD <1 time per year or never did not provide families with nutrition guidelines or resources. Educators trained in Munch & Move, and those who completed PD more frequently, demonstrated better nutrition, physical activity and screen time practices in several areas. SO WHAT?: This study demonstrated benefits of the Munch & Move program, implemented with support from Local Health District health promotion officers, and highlighted key areas for improvement in healthy practices in FDC.

Effects of host size on progeny sex and survivorship of Hymenoepimecis pinheirensis

Abstract Parasitoid larvae consume their hosts to obtain the nutritional resources required for their development. Parasitoid wasps can optimally select the size of their hosts by laying unfertilized and fertilized eggs according to the amount of biomass available for consumption by the larvae. However, parasitoids may eventually experience low host availability within the optimal range of body sizes, affecting the survival of their offspring. In this study, we identified a situation in which all available hosts (Leucauge volupis) were smaller than those previously observed to be parasitized by Hymenoepimecis pinheirensis at the same study site. Therefore, we investigated how these parasitoids can bypass the scarcity of ideal hosts. Female wasps biased their oviposition toward the largest L. volupis females available. In this suboptimal scenario, they did not oviposit only unfertilized eggs, which developed into relatively small offspring (males). In this situation, they lay fertilized eggs mainly on larger spiders. Larval mortality was high, but the larvae attached to the larger spiders were more likely to complete their development. In general, H. pinheirensis females managed to target the best hosts available but could not delay the oviposition of fertilized eggs or avoid offspring mortality. Here, we discuss the potential causes of asynchronies in the life cycles of parasitoid wasps and their hosts, the availability of optimal hosts, and how these factors may affect their populations.

PvMYB60 gene, a candidate for drought tolerance improvement in common bean in a climate change context

BackgroundCommon bean (Phaseolus vulgaris) is one of the main nutritional resources in the world, and a low environmental impact source of protein. However, the majority of its cultivation areas are affected by drought and this scenario is only expected to worsen with climate change. Stomatal closure is one of the most important plant responses to drought and the MYB60 transcription factor is among the key elements regulating stomatal aperture. If targeting and mutating the MYB60 gene of common bean would be a valuable strategy to establish more drought-tolerant beans was therefore investigated.ResultsThe MYB60 gene of common bean, with orthology to the Arabidopsis AtMYB60 gene, was found to have conserved regions with MYB60 typical motifs and architecture. Stomata-specific expression of PvMYB60 was further confirmed by q-RT PCR on organs containing stomata, and stomata-enriched leaf fractions. Further, function of PvMYB60 in promoting stomata aperture was confirmed by complementing the defective phenotype of a previously described Arabidopsis myb60-1 mutant.ConclusionsOur study finally points PvMYB60 as a potential target for obtaining more drought-tolerant common beans in the present context of climate change which would further greatly contribute to food security particularly in drought-prone countries.

Precision feeding as a tool to reduce the environmental footprint of pig production systems: a life-cycle assessment.

Nitrogen and phosphorus excretion are major sources of environmental contamination in growing-finishing pig operations. Nutrient excretion can be reduced by feeding pigs daily-tailored diets to their estimated nutrient requirements using individual precision feeding (IPF) techniques. This study modeled and evaluated the environmental impact of moving from conventional group 3-phase feeding (CGF) to IPF systems in Quebec, Canada, using life-cycle assessment with SimaPro software. The cradle-to-farm model included inputs and outputs of each subphase: raw materials/feedstuffs production, feed mill processing, transport, animal rearing, and manure management. The model was identical for both treatments in all aspects except for the production of feeds and barn and manure emissions in the growing-finishing phases. All feed ingredients originated from Quebec, simulating agricultural practices using real management data from an average farm in Quebec. Based on observed pig growth data, the CGF and IPF systems were compared in the growing-finishing phase. IPF diets were modeled as the blend of 2 feeds (i.e., A and B), while CGF diets were stablished according to the industry. The evaluated impact categories were global warming potential (GWP), eutrophication potential (EP), and acidification potential (AP). The functional unit was 1 t of feed at the feed mill gate and 1 t of finished pig live weight at the farm gate. A Monte Carlo analysis determined the uncertainty of the growth performance results. Feeding programs were compared using analysis of variance. Corn was associated with elevated GWP and AP impacts, leading to higher impacts for diets with higher corn content. Feed B, which contained 83% corn, resulted in impacts of 645kg of CO2-eq., 8.53kg SO2-eq., and 4.89kg PO4-eq. Diets with higher EP impact had a higher percentage of soybean meal. Feed A contained 25% of this ingredient and had an impact of 608kg CO2-eq., 6.98kg SO2-eq., and 5.57kg PO4-eq. CGF diets had environmental impacts between those of feeds A and B. Compared to CGF, applying IPF programs during the growing-finishing phase decreased GWP by 7.6%, AP by 16.2% and EP by 13.0%. IPF significantly reduced the environmental impact in all categories through the more efficient use of nutritional resources by pigs. IPF could help to improve the sustainability of growing-finishing pig operations in Quebec and likely other regions using corn and soybean-based diets.