Department Of Nutrition Research Articles

Reversible Gynecomastia in HIV-Infected Man Treated With Triple Antiretroviral Therapy Containing Efavirenz: A Case Report

Gynecomastia is benign hypertrophy of male breast glandular tissue, either unilateral or bilateral, secondary to increased estrogen/testosterone ratio (elevated estrogen level, decreased testosterone levels, or both). The condition can be related to a medical disease or caused by some drugs.Since the introduction of triple antiretroviral therapy (TAT), we have seen an improvement in the prognosis of human immunodeficiency virus (HIV) infection.Here we report the case of a 53-year-old man receiving follow-up care in Internal Medicine for HIV infection receiving TAT (tenofovir/efavirenz/emtricitabine). After one year, the patient presented in the Department of Endocrinology, Diabetology, Metabolic Diseases, and Nutrition of Hassan II University Hospital Center, Fez, with bilateral gynecomastia. Hormonal exploration did not reveal any abnormality, so the gynecomastia was attributed to efavirenz use. The regimen was replaced by tenofovir, lamivudine, and dolutegravir. The gynecomastia was resolved within two months of discontinuing efavirenz.In summary, we think that secondary gynecomastia should be suspected and screened in HIV patients receiving efavirenz-containing regimens.

Health sciences students' and instructors' perceptions of the emergency switch to virtual internship amid the COVID-19 pandemic: A case from Qatar.

In efforts to contain the COVID-19 pandemic, health colleges at Qatar University shifted their clinical training to virtual internships (VI) and project-based learning (PBL). The shift was new to students and faculty alike, and a major change that posed many challenges. This study aimed to explore the experience of changing to VIs during the pandemic from both the clinical instructors' and health sciences students' perspectives. A qualitative study was conducted based on the framework of readiness to change. It involved focus group discussions with students from the departments of Public Health and Human Nutrition and in-depth interviews with clinical instructors using appropriate online platforms. A total of 4 focus groups with 20 students and 4 interviews with instructors were conducted. Transcripts were analyzed following the inductive-deductive approach. The major themes that emerged from the analysis described students' and clinical instructors' perceptions of the necessity and efficiency of the switch to VI; the design of the VI and the extent of the clinical/field experience and skills that it offered; confidence in the ability to succeed in this type of internship and confidence about reaching expected goals; academic and moral support from clinical faculty and coordinators and the communication process with faculty and preceptors; and finally, the benefits gained and how employers would view this type of internship. Health sciences students' readiness for VI was generally low. Several student and faculty needs have to be addressed, specifically regarding the design of the program and the level of preceptors' communication with students. The findings would direct health programs, clinical instructors, and preceptors to better understand students' needs and efficiently plan for virtual internships during not only emergencies but also whenever there is a need to deliver online experiential learning courses.

Contributors

Other| August 01 2022 Contributors Gastronomica (2022) 22 (3): ix–x. https://doi.org/10.1525/gfc.2022.22.3.ix Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Contributors. Gastronomica 1 August 2022; 22 (3): ix–x. doi: https://doi.org/10.1525/gfc.2022.22.3.ix Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentGastronomica Search Lara Anderson is an associate professor in the Spanish and Latin American Studies program at the University of Melbourne. Her main research area is Spanish food culture, and her recent monograph, Control and Resistance: Food Discourse in Franco Spain (University of Toronto Press, 2020), looked at food discourse as a site of control and resistance. Agata Bachórz is an assistant professor of sociology at the University of Gdańsk, Poland. Her research interests include food cultures and their relation to modernization, reinterpretation of “national cuisines,” and cultural dimensions of food production, mainly in Eastern Europe. Natasha Bunzl is a PhD student at New York University in the Department of Nutrition and Food Studies. Her research focuses on nonprofits that address food insecurity and the increasing presence of celebrity chefs in solutions to hunger. Jaina Cipriano is a Boston-based artist communicating with the world through photography, film, and installation. Her works explore... You do not currently have access to this content.

Beslenme ve Diyetetik Bölümü Öğrencilerinde Tavuk Eti Tüketim Durumu ve Tercihlerinin Belirlenmesi

Tavuk eti artan nüfusun hayvansal protein ihtiyacını karşılayan önemli bir besindir. Özellikle içerdiği esansiyel aminoasitler bakımından biyoyararlanımı yüksektir. Bu çalışmanın amacı üniversite öğrencilerinde tavuk eti tüketim durumu ve tercihleri hakkında bilgi sahibi olmaktır. Çalışma Beslenme ve Diyetetik bölümünde yürütülmüştür. Öğrencilerle gönüllü olarak yüz-yüze anket yöntemiyle araştırma tamamlanmıştır. Öğrencilerin ortanca yaşı 20.0 olup, %92.3’ü kadındır. Çalışmada öğrencilerin %98’inin tavuk eti tükettiği belirlenmiştir. Tavuk eti tüketmeyen öğrencilerin %85.7’si ise tüketmeme nedeni olarak tavuk etini sevmediklerini belirtmişlerdir. Tavuk etinin besleyici değerini bilip bilmedikleri sorusuna ise öğrencilerin %85.2’si olumlu cevap vermiştir. Tercih edilen tavuk eti kısımlarının çoğunlukla göğüs, but, baget ve kanat olduğu ve bunları alırken özellikle son tüketim tarihine (%96.4), markasına (%72.4), ambalajına (%65.3) ve fiyatına (%62.8) önem verildiği tespit edilmiştir. Öğrencilerin işlenmiş tavuk ürünlerini yüksek oranda tercih ettiği (%91.8) ve bu ürünlerden de döner, hamburger, nugget ve şinitzel gibi hazırlaması kolay ürünleri seçtikleri belirlenmiştir. Reyondan tavuk eti alırken organik tavuk tercihinin (%45.4) daha yüksek olduğu bulunmuştur. Tavuk eti alırken, yetiştirilme süresinin önemsenmediği (%65.3) belirlenmiştir. Sonuç olarak, daha önce yapılan çalışmalara benzer şekilde, beslenme ve diyetetik bölümü öğrencilerinin mesleki bilgilerinin de katkısıyla tavuk etini kısa sürede tüketime sunulabilme kolaylığı, ulaşılabilirliği gibi nedenlerle sıklıkla tercih ettikleri belirlenmiştir. Bu nedenle üretimde yer alan işletmeler öğrencilerin tüketim tercihlerini de dikkate alarak ürünler geliştirebilirler.

Manejo hospitalario del paciente con disfagia. Encuesta y recomendaciones del área de nutrición de la SEEN

IntroductionThe prevalence of dysphagia in hospitalized patients is extraordinarily high and little known. The goal of care should be to assess the efficacy and safety of swallowing, to indicate personalized nutritional therapy. The development of Dysphagia Units, as a multidisciplinary team, facilitates comprehensive care for this type of patient. Material and methodsA observational, cross-sectional, web-based survey-type study, focused on Spanish Society of Endocrinology and Nutrition department heads, was conducted in September-October 2021. The following data were analyzed: size and type of center, existence of a dysphagia unit, dysphagia screening, dietary and nutritional therapy, education and training of professionals and patients, codification, and quality of life evaluation. Results65 responses (39% of the total Endocrinology and Nutrition departments). 37% of hospitals have a Dysphagia Unit and 25% are developing it. 75.4% perform screening, with MECV-V in 80.6%, and VED (61.4%) and VFS (54.4%) are performed as main complementary tests. The centers have different models of oral diet, thickeners and nutritional oral supplements adapted to dysphagia. In 40% of the centers, no information is offered on dysphagia, nor on the use of thickeners, dysphagia is coded in 81%, 52.3% have specific nursing protocols and only 8% have scales for quality-of-life evaluation. ConclusionsThe high prevalence and the risk of serious complications require early and multidisciplinary management at the hospital level. The information received by the patient and caregiver about the dietary adaptations they need, is essential to minimize risks and improve quality of life.

Analysis of Vegetable Nutritional Garden Interventions to Improve Socio-economic Status, Nutritional and Livelihood Security of Selected Beneficiaries under DST- WOS-B Project of District Moga, Punjab, India

Study was carried out in Punjab Agricultural University, Ludhiana, Punjab to analyse the impact of vegetable nutritional garden on the nutritional intake and socio- economic status of selected beneficiaries of District Moga, Punjab, India. Survey conducted to assess the impact of DST Project from 82 beneficiaries across the two blocks i.e. Village Khosapando and Niddhawala, Village Dagaru and Churchak from November- December, 2019 and again August-September, 2021, through a structured questionnaire developed by the Department of Food and Nutrition. The data pertaining to general profile, dietary habits, socio-economic status of the beneficiaries.
 The dietary diversity questionnaire include 12 groups of food like Cereals, Pulses, Green leafy vegetables, Roots, Tubers, Fruits, Vegetables, Milk and Milk products, Egg, Fat, Sugar, Meat and Miscellaneous. The information on respondent’s food consumption was collected using (24-hour recall).The results have shown that, there was difference in the nutritional uptake of beneficiaries during the intervention period and before the intervention of project. Under this project, area under vegetable cultivation has increased significantly in all selected villages over period 2019-2022 i.e. area under vegetable production in Village Khosa Pando, Dagru, Chuharchak and Nidhanwala increased by 91.6,100, 93.9, 94.2 percent. Moreover, per capita income of farmers having landholding of 0.5 to 1 acre and 0.1 to 0.5 acre has increased by 180 and 62 percent respectively. Furthermore, the consumption of green and leafy vegetables, roots and tubers, and other vegetables by intervention household increased drastically from 61.4, 48, 54 to 97.6, 95.0 and 88.1 respectively after establishing vegetable nutrition gardens.

The Relationship Between Chronotype and Impulsivity, Attention-Deficit Disorder, Internet, Social Media, and Smartphone Addiction.

The aim of our study is to investigate the relationship between chronotypes and impulsivity, attention-deficit disorder and smartphone, social media, and internet addiction in a sample of university students. The study was conducted at our university from May to July 2021. A total of 255 subjects were recruited in the study among the students from the medical faculty and the faculty of health sciences (departments of nutrition and dietetics, nursing, and physiotherapy and rehabilitation) of our university who received face-to-face education in 2020-2021. Sociodemographic data form, the Adult Attention-Deficit/Hyperactivity Disorder Self-Report Scale, Morningness-Eveningness Questionnaire, Barratt Impulsiveness Scale-11 Short Form, Young's Internet Addiction Scale, Social Media Addiction Scale-Adult Form, and Smartphone Addiction Scale-Short Version were administered by the study investigators through face-to-face interviews. Among the students, the intermediate type was most common (n = 157) and the evening type was least common (n = 44). The chronotypes differed in terms of the time spent daily on smartphone and internet (P = .001 and P < .001). The evening types showed significantly higher mean scores on the Adult Attention-Deficit/Hyperactivity Disorder Self-Report Scale, Morningness-Eveningness Questionnaire, Barratt Impulsiveness Scale-11 Short Form, Young's Internet Addiction Scale, Social Media Addiction Scale-Adult Form, and Smartphone Addiction Scale-Short Version when compared with other chronotypes. Female and male students showed comparable scores on all scales. Chronotypes were not different between genders. The results of our study showed a greater frequency of smartphone, internet, and social media addiction among the evening types than in the other chronotypes. Greater impulsivity and attention problems as observed in the evening types may be predisposing these individuals to develop addiction to smartphone, internet, and social media.

Assessment of the Relationship Between University Students' Food Consumption and Anthropometric Measurements and Phase Angle

Purpose: This study was carried out to assess the relationship between university students' food consumption and anthropometric measurements, and phase angle (PA).&#x0D; Methods: The study was conducted with a total of 240 students between the ages of 18-25 studying at the Department of Nutrition and Dietetics at XXX University Health School. Research data were collected using a questionnaire that included about demographic information and questions about physical activity levels and three-day food consumption record was taken. Lastly, anthropometric measurements of the students were performed by the researcher in person.&#x0D; Results: The study's findings revealed that the students' energy intake was lower than their energy expenditures. The body mass indexes (BMI), and waist circumferences of female and male students were calculated as 21.8 ± 3.2 kg/m2 and 23 ± 3.4 kg/m2, and 75.2 ± 8.1 cm and 85 ± 10 cm, respectively. The PA was 5.7 ± 0.5º in female students and 6.7 ± 0.5º in male students (p

Vegetarianism and weight status: What are the differences in eating styles, impulsivity, and emotional competences? A preliminary study

Recent studies suggest that vegetarian diets may be recommended to promote weight loss in individuals living with obesity. However, limited studies have examined psychological factors (e.g., eating styles, impulsivity) among individuals who have adopted this type of diet, even though these factors are known to play a role in being overweight. The primary objective of the present study was to compare these characteristics in participants living with obesity or those with normal-weight across diet types. Participants were recruited from two hospital nutrition departments and the general population. They completed a diagnostic interview assessing the presence of an eating disorder, followed by self-administered questionnaires measuring dysfunctional eating styles (DEBQ), impulsivity (UPPS), and emotional competence (PEC). Vegetarian participants living with obesity engaged in more dysfunctional eating styles than did normal-weight omnivores and experienced more emotional difficulties than did both normal-weight omnivores and vegetarians. In contrast, there were no significant differences between omnivore participants living with obesity and those in the other groups. Moreover, participants living with obesity had comparable emotion regulation abilities to normal-weight participants. These results suggest that emotion regulation deficits can more likely be explained by the presence of psychopathological traits than by being overweight or one’s choice of diet.

P153 Spatial Analysis of Perinatal Food Assistance Resources in Relation to Food Deserts in North Carolina

<h3>Background</h3> A number of risk factors can influence maternal and infant health including chronic conditions, health behaviors and prenatal care. Women with access to perinatal resources and healthy foods could limit the impact of these risk factors for the maternal and infant well-being. Geographic Information Systems (GIS) is a software used to capture, store, and analyze geographic data. The advantages of GIS allows understanding of maternal and perinatal food assistance gaps. <h3>Objectives</h3> To locate and map government food assistance perinatal resources such as WIC, SNAP Offices, WIC and SNAP approved vendors throughout North Carolina. Determine if there are spatial correlations between the density (ie number of physical locations at the census tract level) of food assistance clinics and approved retailers in comparison to areas classified as food deserts by the USDA (low access and low income designation). Assess the association between underlying population characteristics and WIC/SNAP locations. <h3>Study Design, Setting, Participants</h3> Undergraduate research assistants from Appalachian State University's Nutrition Department compiled a comprehensive list of WIC clinics, WIC-approved vendors, SNAP offices and SNAP retailers available in North Carolina from Google™. <h3>Measurable Outcome/Analysis</h3> These resources were geocoded to create a spatial distribution of the locations into ArcGIS Pro by ESRI by Appalachian State University's Geography/Planning Department. WIC and SNAP locations were spatially merged with USDA (food desert classification) and American Community Survey Data to create a relational GIS database. Bivariate map products and Kendall tau correlations were used to assess the association between underlying population characteristics and WIC/SNAP locations. <h3>Results</h3> Preliminary findings show limited WIC and SNAP office and retailer locations in rural, underserved locations with food deserts in NC. Spatially, variations in this association varies across the state with limited access in the far Eastern and Western regions of NC. <h3>Conclusions</h3> GIS and other simple spatial analysis allow for critical evaluation of WIC and SNAP offices/retailers and their relationship to food deserts, which could inform policies around support for more offices/retailers. <h3>Funding</h3> Internal Grant from University Research Council

Effect of Purple Yam (Dioscorea alata) and Crookneck pumpkin (Cucurbita moschata) Substitution on Organoleptic and Chemical Properties of Dodol

Dodol is a semi-wet food made from glutinous rice flour, coconut milk, and sugar, occasionally added with permitted food ingredients and other food additives. Purple yam and crookneck pumpkin can be added to dodol to change it. The objective is to determine how the substitution of purple yam and crookneck pumpkin may affect the chemical and organoleptic characteristics of dodol. The panelists used in this study were semi-panelists. This type of research is experimental with a completely randomized design (CRD), specifically 3 treatments and 3 repetitions using the hedonic test. Up to 30 students were trained, originating from the Department of Nutrition, Ministry of Health, Aceh. Utilizing the ANOVA (Analysis of Variance) and Duncan's New Multiple Range tests, data processing and analysis were performed. The most liked dodol is dodol with the addition of 80 g of purple yam and 40 g of crookneck pumpkin. Dodol organoleptic test results include taste, aroma, texture, and color. The proximate test findings demonstrated that the addition of 40g of crookneck pumpkin and 80g of purple yam had an impact on the levels of fat and carbohydrates, and had a significant impact on the color, taste, aroma, and texture. It also had a significant impact on the proximate test of the food's fat and carbohydrate content. According to the researcher's recommendations, the dodol should be cooked for a longer period and with more glutinous rice flour in order to reduce its water content and extend its shelf life.

"Designing, Developing and Optimizing a Two-Stage Solid State Fermentation Process through valorization of Cereal Milling and Legume By-Products and Waste Streams for the Production of Novel Functional Protein Rich Type-Miso Nutri-Powder, Using Aspergillus Oryzae"

Antonios E Koutelidakis, Christina Dimou, Nidopoulou Konstantina and Charalampia M Dimou* Author Affiliations Department of Food Science and Nutrition, Unit of Nutrition, University of the Aegean, School of the Environment, Greece Received: June 14, 2022 | Published: June 22, 2022 Corresponding author: Charalampia M Dimou, Human Nutrition Unit, Laboratory of Nutrition and Public Health, Food Science and Nutrition Department, University of the Aegean, 11472 Myrina, Greece DOI: 10.26717/BJSTR.2022.44.007093

Standardization of ethnic fermented sour beverages by indigenous red rice varieties and its nutritional characteristics

Department of Food Science and Nutrition, Community Science College and Research Institute, Madurai-625104.

Antimicrobial Peptides in Bladder Cancer

Novel Approaches in Cancer Study Antimicrobial Peptides in Bladder Cancer Cüneyd Yavaş1, Ster Irmak Sav2* and Nehir Özdemir Özgentürk1 1Department of Molecular Biology and Genetics, Turkey 2Department of Nutrition and Dietetics, Turkey *Corresponding author: Ster Irmak Sav, Department of Nutrition and Dietetics, Turkey Submission: June 9, 2022 Published: June 22, 2022 DOI: 10.31031/NACS.2022.07.000657 ISSN:2637-773XVolume7 Issue2

Examining The Clinical Nutrition Internship Effectiveness from Interns' Perceptions

Through internship programs, students can acquire practical experience while learning from experts. Students can gain hands-on experience while combining theory and practice. This study examines the clinical nutrition internship program at the University of Hail in Saudi Arabia. Another objective of this study is to examine how interns perceive the internship program. Clinical nutrition students were surveyed online using social media platforms and specialized groups. This study involved 40 clinical nutrition interns in 2022/3/7. All of the interns were from the University of Hail. In this study, interns indicated that the internship positively impacted their learning, interpersonal, and personal skills and their employment prospects. For instance, 50% reported the availability of site supervisors when needed. Furthermore, 90% of the respondents indicated having the ability to persevere in complex tasks. Moreover, 90% illustrated that they acquired knowledge from the job duties. Further research is required to identify the barriers associated with this issue. According to the researchers, clinical nutrition, and other departments in the college of applied medical sciences curriculum should integrate soft skills. The supervisor should have a thorough understanding of the health informatics field and the intern's responsibilities. This will enable them to help the intern put what they learned in class into practice. The findings of this study can be used to study health informatics internships in greater depth. We strongly recommend conducting a comparative study with more participants from diverse institutions.

Analysis of Change in Body Composition in Diabetic Patients vs non-Diabetic Patients

Objective: To assess the difference in various parameters of body composition between the diabetic patients and non-diabetic controls at Pak Emirates Military Hospital Rawalpindi.&#x0D; Study Design: Comparative cross-sectional study.&#x0D; Place and Duration of Study: Medicine and Nutrition Department, Pak Emirates Military Hospital, Rawalpindi, from Jul 2018 to Mar 2019.&#x0D; Methodology: This study was conducted on 100 patients of non-insulin dependent diabetes mellitus under treatment in medicine department and matched controls from the community. Patients with type I diabetes or insulin dependents or having some other com or bid metabolic illness or autoimmune disease or malignancy were not included in the study. Parameters of body composition were measured by using the INBODY machine which works on the principle of electroimpedance.&#x0D; Results: Cases and controls were matched for age and gender. Hundred cases and hundred controls were recruited for the final analysis Mean age of the cases was 43.41 ± 2.231 years while controls were 43.46 ± 3.122 years. Mean BMI in cases was 29.32 ± 2.561while in controls was 25.62 ± 4.483. BMI, visceral fat and skeletal muscle fat were found statistically significant to be more in cases of non-insulin dependent diabetes mellitus as compared to the controls.&#x0D; Conclusion: This case control study revealed that diabetes significantly affects the body composition of the patients. It also affects other parameters like skeletal muscle mass and visceral fat. Routine screening of body composition should be made part of the diabetic clinics to pick these changes early and intervene accordingly.

Medical Nutrition Therapy in Patient With Multiple Fractures, and Liver Injury With Hypoalbuminemia Complications and Moderate Protein Energy Malnutrition: A Case Report

ObjectivesThe mandibular bone is a place of attachment for the muscles of mastication that are actively moving so that it can make it difficult for the patient to carry out the process of chewing food.MethodsA 29 years old male with diagnosis multiple fracture due to a traffic accident. The patient experienced a decrease in oral food worsened 2 days ago because he was difficult to open his mouth due to severe pain when opening his mouth. Looks like a post-surgery wound Mandibular ORIF on the left side of the mandibular region, postoperatively. Exploratory Laparotomy + Liver Repair” in the abdominal. On laboratory examination found the metabolic status Normochromic Normocytic Anemia (11,4), leukocytosis (13,600), thrombocytopenia (143,000), increased NLR (5.42), elevated transaminase enzymes (543/675), Azotemia (1,44), mild hypoalbuminemia (3,0).ResultsMedical nutrition therapy is given gradually according to the patient's eating tolerance, starting from giving 1350 Kcal via oral in the form of semi-liquid food because the patient still has difficulty chewing there until it reaches 2700 Kcal which is assisted by parenteral nutrition. The composition of the macronutrients given. The patient was given oral supplementation in the form of zinc, curcuma, Vitamin A, C, D and snakehead fish extract capsules. The patient was discharged after 12 days of treatment with improved food intake (achieved 100% KET), there was no decrease in anthropometric parameters, the postoperative wound looked dry and improved metabolic status in the form of a decrease in leukocytes towards normal (8.3 × 103/mL), improvement in blood levels platelets (438.000), immune system improvement (1402.9/mL), liver function improvement (43/95), Improvement of kidney function (0.57).ConclusionsOptimal postoperative wound care and adequate medical nutrition therapy with dietary modifications can improve the patient's clinical condition including reducing the inflammatory reaction that occurs and improving the quality of life of patients treated with postoperative repair of mandibular fractures and fractures of the radius/ulna and liver injury due to blunt abdominal trauma.Funding SourcesInstitutions from Department of Nutrition, Faculty of Medicine, Hasanuddin University.

Postoperative Medical Nutrition Therapy in Patient With Ascending Colon Adenocarcinoma With Severe Malnutrition: A Case Report

ObjectivesColorectal cancer is one of the cancers with the highest cause of death in the world. Indonesia as a developing country is also reported an increased incidence of colorectal cancer together with the increase in the economy, industrialization, and westernization lifestyle. Due to the inflammatory process, colorectal cancer patients are in a hypermetabolic state leading to increased nutritional requirements and failure to meet these requirements will result in malnutrition. Colorectal cancer with severe malnutrition requires special nutrition therapy, tailored to each patient based on their nutritional status and clinical condition.MethodsA 36 year old male patient with severe malnutrition (subjective global assessment score C) diagnosed with post exploratory laparotomy, adhesiolysis, segmentectomy, end ileostomy due to adenocarcinoma of the ascending colon. Oral intake is decreased due to loss of appetite and abdominal pain. The patient shows pallor conjunctiva, loss of subcutaneous fat, and wasting of the extremities. Laboratory findings were anemia (10.4 g/dL), leukocytosis (20,100 mm3), total lymphocyte count (582.9/µL), hypoalbuminemia (1.8 gr/dL), hyponatremia (132 mmol/L), elevated enzymes transaminases (SGOT 232 U/L, SGPT 88 U/L).ResultsMedical nutrition therapy was initially given 450 kcal and gradually increased to 2300 kcal, protein 1–1.8 g/kilogram ideal body weight/day using a high protein formula and Branched Chain Amino Acid (BCAA) parenteral nutrition. We administered supplementations which were zinc, vitamin A, B, C, D, curcuma, and snakehead fish extract. The patient was discharged after 12 days with clinical and functional improvement assessed with handgrip strength dynamometer from 11.4 to 16.3 kg. Laboratory improvement were leukocytes 10,000 mm3, total lymphocyte count 1,310/µL, albumin 2.7 gr/dL, sodium 133 mmo/L, SGOT 40 U/L, SGPT 31 U/L.ConclusionsMalnourished colorectal cancer patient had a higher risk of poor clinical outcome. Specific nutritional therapy is needed to reduce inflammation or hypermetabolic, and to treat postoperative complications, proper monitoring and nutritional education results in a good outcome for patient.Funding SourcesInstitutions from Department of Nutrition, Faculty of Medicine, Hasanuddin University.

Prebiotics for Chemotherapy-Induced Lower Intestinal Mucositis (CIM) With Moderate Protein Energy Malnutrition: A Case Report

ObjectivesChemotherapy-induced mucositis (CIM) is the most common side effect of chemotherapy. In addition, it reduces quality of life and leads to a reduction in chemotherapy doses, and eventually increasing mortality rate. CIM can cause malnutrition. Chemotherapy disrupts the symbiotic relationship between the microbiota and the patient. Various modalities are able to simulate colonization of probiotic bacteria in the intestine that have potential to be a strategy to restore healthy gut microbiota. Such as prebiotic.MethodsA 42-years old male with moderately malnourished (Subjective Global Assessment score B, Unintentional weight loss for 5 months (±7%) and body mass index was about 22.94 kg/m2) with diagnosis of Non-Hodgkin Malignant Lymphoma, Sinonasal Carcinoma and chemotherapy induced acute kidney injury. He had Leukopenia (2800/ul), decrease of kidney function (ureum/creatinine 28/1.5 mg/ul) and lymphopenia (926.8 ul).ResultsThe administration of nutrition therapy was initiated from 1000 kcal and increased gradually with total energy expenditure target of 2500 kcal via oral and parenteral nutrition, protein 1.2–1.5 g/Ideal Body Weight/day (15,3%) using high protein formula and amino acids parenteral nutrition. We administered prebiotics with a dose 5 gr/day for 6 weeks. Multivitamin, minerals and nutrition supplementations were administered; which were vitamin A, B1, B2, B6, B12, C, Folic acid, Zinc, Snakehead fish extract, Curcuma extract. During 13 days of nutritional therapy treatment, he achieved an average of 93,6% and 74% of energy and protein target, respectively. His clinical, functional capacity, laboratory were improved. Gastrointestinal symptoms such as nausea, vomiting, sore throat and constipation were improved and chemotherapy dose continued.ConclusionsClinical improvement such as increased oral intake after the prebiotics had given. Therefore, prebiotics had a potential benefit for CIM.Funding SourcesInstitutions from Department of Nutrition, Faculty of Medicine, Hasanuddin University.

Bifidobacterium longum subsp. infantis Shifts Global Transcriptional and Post-translational Modification Signals in Preterm Infant Gut Epithelial Cells

Objectives Bifidobacterium longum subsp. infantis (B. infantis) is an important human infant gut symbiont that confers benefits to the infant by shaping the gut microbiome in vivo. Here, we investigated the transcriptomic and post-translational modification (PTM) impacts of B. infantis on host gut epithelial cells representative of preterm infants, in vitro.MethodsRNA isolated from preterm infant intestinal epithelial cells (HIEC-6) exposed to sterile, cell-free B. infantis supernatants or controls (no supernatant) for 20 hours was subjected to RNA-sequencing to assess global transcriptional changes (n = 6/group). Using the same approach, HIEC-6 cells were incubated for 3 or 20 hours, with or without supernatant (10% v/v; n = 3/group), before harvest for cellular proteins and subsequent western blotting. Gels were probed for α-tubulin, acetylated α-tubulin, Extracellular signal-Regulated Kinase (ERK), and phosphorylated ERK as markers of PTM activity.ResultsGlobal transcriptomic profiles were significantly different between cells exposed to supernatant and those that were not (P = 0.006) and gene pathways involved in anti-inflammatory responses and chemotaxis were significantly upregulated (P < 0.01). Western blotting revealed significant increases in acetyl-α-tubulin and phospho-ERK in cells exposed to supernatant relative to the control group (P < 0.05), while total α-tubulin and ERK concentrations were not significantly different (P > 0.05).ConclusionsThese findings demonstrate the ability of B. infantis to alter global transcriptional and PTM responses affecting inflammation, intracellular trafficking of α-tubulin, and transcription factor recruitment by ERK phosphorylation. These pathways regulate key cellular processes such as cell proliferation, differentiation, adhesion, migration and survival, providing molecular insights into the ability of B. infantis to regulate transcriptional and PTM processes in vitro.Funding SourcesThis work was funded by the Department of Nutrition, the College of Agriculture, Biotechnology, & Natural Resources, the Nevada Agricultural Experiment Station, and the Vice President for Research and Innovation, as well as the NICHD, R03HD105881, and grants from the NIGMS (GM103440 and GM104944) from the NIH.