Number Of Bacterial Colonies Research Articles

Antibacterial Potential of Freshener Water Based on Siwalan Coir Extract (Borassus flabellifer) and Lemongrass (Cymbopogon citratus) Against Airborne Bacteria

Air is an important part of life. The Quality of air can be seen from three parameters, namely chemical, physical, and biological parameters. One of the biological parameters of air quality can be seen from the presence of microorganisms such as bacteria. The presence of airborne microorganisms, especially in enclosed spaces can affect human health, one of which can cause respiratory infections. On the other hand, people often use air freshener products. Therefore, this research was conducted with the aim of analyzing the potential of air freshener based on siwalan (Borasus flabellifer) coir extract and lemongrass as antibacterial against airborne bacteria. This research is an experimental research with a pre test-post test design. The data obtained is in the form of the value of the Total Plate Count of indoor air bacteria before and after treatment using air freshener based on coir extract of siwalan and lemongrass. The results showed that both formulations (P1 and P2) of air freshener based on coir extract and lemongrass were able to reduce the number of airborne bacterial colonies in the room, with a significance value of 0.000<0.05.

Fabrication of smart film based on fish gelatin incorporating phycoerythrin and cellulose nanofibrils to monitor fish freshness

Phycoerythrin (PE) extract from red algae Laver (Pyropia dentata) was used in the fabrication of a smart packaging film. The PE showed pH sensitivity, implying unique color shifts at different pH. The film colors altered from red through purple-brown to colorless with increasing pH. The smart packaging films were prepared by casting method composed of gelatin (GE), sodium alginate (SA), and cellulose nanofibrils (CNF). The films were coded as GE/SA (control), GE/SA/PE, and GE/SA/CNF/PE. The thickness of the films ranged from 0.24 ± 0.02 mm to 0.33 ± 0.01 mm. The mechanical properties tensile strength (TS) (12.24 ± 0.30 MPa to 17.65 ± 0.33 MPa) and elongation at break (EAB) (79.34% ± 1.02%–93.36% ± 0.69%) significantly increased after addition of CNF. The synthesized films showed water contact angle ranged from 84.55 ± 2.12 to 69.41 ± 1.21, suggesting that films were hydrophobic. The Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis demonstrated strong interaction among PE, CNF, and biopolymers. The film with GE/SA/CNF/PE exhibited higher thermal stability compared with control film. Then, the films were applied for checking real time application for the freshness of salmon fish. The significant color change of the film occurred and the number of bacterial colony and TVB-N were increased from 3.81 Log CFU/g to 6.94 Log CFU/g and from 4.78 mg/100 g to 32.27 mg/100 g after incubation for 48 h. Therefore, the film with PE and CNF was intended as an excellent candidate for smart packaging film.

Based on molecular docking and real-time PCR technology, the two-component system Bae SR was investigated on the mechanism of drug resistance in CRAB.

This study aimed to explore the role of the two-component system Bae SR in the mechanism of drug resistance in carbapenem-resistant A. baumannii (CRAB) using molecular docking and real-time polymerase chain reaction (PCR). The two-component system Bae SR of Acinetobacter baumannii was subjected to molecular docking with imipenem, meropenem, and levofloxacin. Antibacterial assays and fluorescence quantitative PCR were used to explore protein-ligand interactions and molecular biological resistance mechanisms related to CRAB. The analysis of the two-component system in A. baumannii revealed that imipenem exhibited the highest docking energy in Bae S at - 5.81kcal/mol, while the docking energy for meropenem was - 4.92kcal/mol. For Bae R, imipenem had a maximum docking energy of - 4.28kcal/mol, compared with - 4.60kcal/mol for meropenem. The highest binding energies for Bae S-levofloxacin and Bae R-levofloxacin were - 3.60 and - 3.65kcal/mol, respectively. All imipenem-resistant strains had minimum inhibitory concentration (MIC) values of 16µg/mL, whereas levofloxacin-resistant strains had MIC values of 8µg/mL. The time-sterilization curve showed a significant decrease in bacterial colony numbers at 2h under the action of 8µg/mL imipenem, indicating antibacterial effects. In contrast, levofloxacin did not exhibit any antibacterial activity. Fluorescence quantitative PCR results revealed significantly increased relative expression levels of bae S and bae R genes in the CRAB group, which were 2 and 1.5 times higher than those in the CSAB group, respectively, with statistically significant differences. Molecular docking in this study found that the combination of Bae SR and carbapenem antibiotics (imipenem, meropenem) exhibited stronger affinity and stability compared with levofloxacin. Moreover, the overexpression of the two-component system genes in carbapenem-resistant A. baumannii enhanced its resistance to carbapenem, providing theoretical and practical insights into carbapenem resistance in respiratory tract infections caused by A. baumannii.

Anticaries and Antigingivitis Properties of Cannabinoid-Containing Oral Health Products: A Review.

To evaluate the anticaries and antigingivitis properties of cannabinoid-containing oral health products. A systematic research strategy was employed. Specific search terms were used, including "Cannabinoids AND dental caries," "Cannabinoids AND oral health," "Cannabinoids AND dental plaque," "Cannabinoids AND gingivitis AND periodontitis," "Cannabinoids AND S. mutans," "Cannabidiol AND oral health," and "Cannabidiol AND oral biofilm." The search was conducted in PubMed, Cochrane, and EBSCO Host databases. The search yielded a total of 73 articles, out of which 15 articles (20.5%) were relevant to the scope of this systematic review. Among the relevant articles, only eight (10.9%) directly addressed the research question. The findings from these articles suggest that cannabinoids have the potential to reduce the metabolism of cariogenic bacteria, specifically Streptococcus mutans, and decrease the number of bacterial colonies in dental plaque. In vitro studies also demonstrated a significant inhibitory effect of cannabinoids on oral biofilms and create a considerable inhibitory zone of growth when investigated on oral biofilms in vitro. Furthermore, CBD exhibited antibacterial properties against Porphyromonas gingivalis, a primary pathogen associated with periodontal disease. The current review shows insufficient data to conclude on the anticaries and antigingivitis effects of cannabinoids. Despite extensive research on their systemic therapeutic benefits, their oral health impact remains underexplored, lacking clinical trials and primary research.

Local Production of World Health Organization Recommended Hand Rub Formulation and Comparison of its Efficacy with a Commercially Available Alcohol-Based Hand Rub

Objective: To produce a World Health Organization (WHO)-recommended hand rub formulation locally and compare its efficacy with a commercially available Alcohol-based Hand Rub (ABHR) - Sterillium. Methods: A non-randomized comparative study was conducted. Sixty healthcare workers were divided into two groups - Group A (WHO-recommended hand rub) and Group B (Sterillium). WHO-recommended hand rub was prepared according to the specified formula. Each participant from study Group A received three ml of WHO-recommended hand rub formulation, and each study Group B participant received three ml of Sterillium. Bacterial samples of each participant (from both hands) were collected before and 0 after using an ABHR solution. Samples were inoculated on the nutrient agar plates and incubated at 37 C for 24 hours. The total number of bacterial colonies grown on plates inoculated before and after ABHR use was counted and identified by standard microbiology techniques and compared between the two groups. The WHO-recommended hand rub and Sterillium were compared and analyzed using differences in proportion. Results: The overall reduction in bacterial growth was comparable for both the hand rubs, with an average load reduction of log 1.5607 for locally produced hand rub solution and an average load reduction of log 1.609 in the case of Sterillium. Conclusion: Both of the handrubs are comparable in efficacy and are effective against all the bacteria isolated from the palms of HCWs (Coagulase Negative Staphylococcus (CoNS), Micrococcus spp., Acinetobacter spp., Bacillus spp., Pseudomonas spp., and Citrobacter), including Methicillin-Sensitive Staphylococcus aureus

Kinetics of Deterioration of the Quality of Purebred Chicken Egg Meal During Storage

The research objective to determine quality of purebred chicken egg meal deteriorates during storage. The research method used experimental method, counting was a completely randomized design consisting of 5 treatments and 4 replications. As a treatment, the storage time for purebred chicken egg flour at room temperature is formulated as follows T0 (0 days), T1 (10 days), T2 (20 days), T3 (30 days) and T4 (40 days). The variables observed were water content, pH and total microbes. The results of analysis of variance showed that storage time at room temperature had a highly significant different effect (P<0.01) on the water content, pH and total microbes of purebred chicken egg flour. The results of the orthogonal polynomial test show that storage time at room temperature has a highly different linear effect on water content obtained by the equation Y=6.561+0.0177X, linear and quadratic on pH obtained by the equation Y=7.64+0.0095X+0.00015X2 and total microbial colonies of purebred chicken egg flour obtained by the equation Y=2686-0.0172X+0.0006X2. It can be concluded that the kinetics of deterioration in the quality of purebred chicken egg flour occurs 0.25% increase for water content and 0.25 increase for pH and 0.24 log cfu/g increase in the number of bacterial colonies during storage.

Conformation Effect on the Mechanical and Microbiological Behavior of Invisible Orthodontic Aligners.

Invisible orthodontic aligners are having a great impact on tooth movement in an aesthetic and effective way. Different techniques, models, and clinical aspects have been studied for their proper use. However, the aim of this research has been to determine the effect of the shaping process on mechanical properties and their bacterial behavior. For this study, 40 original polyurethane plates and 40 identical models, obtained by hot forming the original plates, were used. The static tensile mechanical properties were studied with a Zwick testing machine using testing speeds of 5 mm/min at a temperature of 37 °C. The original plate and the aligner have been studied with a creep test by subjecting the samples to a constant tension of 30 N, and determining the elongation using a long-distance, high-resolution microscope at different time periods between 1 and 720 h. Studies of water absorption has been realized with artificial saliva for 5 h. Bacterial cultures of Streptococcus oralis and Actinomyces viscosus strains were grown on the original plates and on new and used models, to determine the proliferation of each bacterium through metabolic activity, colony-forming units, and LIVE/DEAD assays. The mechanical results showed an increase in the strength of the inserts with respect to the models obtained from 3.44 to 3.95 MPa in the elastic limit and a lower deformation capacity. It has been proven that the transition zone in the creep curves lasts longer in the original plate, producing the rapid increase in deformation at a shorter time (400 h) in the aligner. Therefore, the shaping process reduces the time of dental correction exerted by the aligner. The results of the bacterial culture assays show an increase in the number of bacterial colonies when the aligners have been used and when the polyurethane is conformed due to the internal energy of the model, with respect to the original polyurethane. It has been observed that between the original plate and the aligner there are no statistically significant differences in water absorption and therefore the forming process does not affect water absorption. A slight increase in water absorption can be observed, but after five hours of exposure, the increase is very small.

DETECTION OF BACTERIAL CONTAMINANTS IN READY-TO-DRINK BEVERAGE (ZOBO) SOLD ON FEDERAL UNIVERSITY DUTSE CAMPUS, NORTHWEST NIGERIA

Introduction: Consumption of ready-to-drink beverages is a norm in the world over. However, the presence of total coliform in any beverage renders it unfit for human consumption. Objectives: Due to non-existence of scientific data on the safety status of the ready-to-drink Zobo which is a popular local beverage, we did conduct this study to assess the bacteriological quality of the lots sold to consumers on the campus of Federal University Dutse (FUD), Nigeria. Methods: Purposive sampling was employed to sample nine (9) locally packaged Zobo in three (3) different outlets; FUD Bakery (BAK) where three (3) were assayed (BAKA, BAKB, and BAKC), FUD Backside (BS) where three (3) samples were assayed (BSA, BSB, and BSC), and Morocco Girls’ hostel (MGH) three (3) samples were assayed (MGHA, MGHB, and MGHC). Standard methods were used to assay the sampled Zobo. Results: Results obtained indicate that samples from MGH (8.0×10⁴ CFU/mL) had the lowest total viable count (TVC), while samples from BAK (2.8×10⁷ CFU/mL) recorded the highest TVC. However, samples assayed from MGH recorded no total coliform count. The number of bacterial colonies recorded in BAKA and BAKB was not significantly different from each other (p>0.05). However, the number of bacterial colonies recorded from MGHA was significantly different from those obtained in MGHB and MGHC (p<0.05). Conclusion: Due to the results obtained, we can conclude that Zobo samples assayed in the sampling points were not fit for human consumption during the conduct of our study.

Pre-treatments of <i>Mirasolia diversifolia</i> using <i>Lactobacillus bulgaricus</i> at different dosages and fermentation times: Phytic acid concentration, enzyme activity, and fermentation characteristics

The present study aimed to determine the optimal dosage and fermentation time of Mirasolia diversifolia (leaves and stems) using Lactobacillus bulgaricus bacteria by observing the reduction in phytic acid, the activity of enzymes, pH of fermentation, number of bacterial colonies, and tannin concentration. An experiment was conducted using a completely randomized design (CRD) and 2×3 factorial design with three replications. The treatment factors were Factor A (L. bulgaricus dosages): A1 = 2% (g/v), A2 = 3% (g/v); and Factor B (fermentation times): B1 = 1 d, B2 = 3 d, B3 = 5 d. The dosage of L. bulgaricus and fermentation time had an interaction on phytic acid and its degradation, enzyme activities, pH fermentation, and the number of bacterial colonies. Tannin concentration was not affected. Mirasolia diversifolia fermented with 3% of L. bulgaricus for 5 d resulted in the lowest phytic acid content. Future research requires evaluating in vitro and in vivo dietary formulations for cattle using M. diversifolia fermented with L. bulgaricus.

Comparing the Effects of Two Culture Methods to Determine the Total Heterotrophic Bacterial Colony Count in Hospital Purified Water

BackgroundAccurately detecting the quantity of microorganisms in hospital purified water is of significant importance for early identification of microbial contamination and reducing the occurrence of water-borne hospital infections. The choice of detection method is a prerequisite for ensuring accurate results. Traditional Plate Count Agar (PCA) belongs to a high-nutrient medium, and there may be limitations in terms of accuracy or sensitivity in detecting microorganisms in hospital purified water. On the other hand, Reasoner’s 2A agar (R2A) has characteristics, such as low-nutrient levels, low cultivation temperature, and extended incubation time, providing advantages in promoting the growth of aquatic microorganisms. This study, through comparing the differences in total colony counts between two detection methods, aims to select the method more suitable for the growth of aquatic microorganisms, offering new practical insights for accurately detecting the total count of heterotrophic bacteria in hospital purified water.MethodsThe most commonly used plate count agar (PCA) method, and the R2A agar culture were adopted to detect microorganisms and determine the total number of bacterial colonies in the water for oral diagnosis and treatment water and terminal rinse water for endoscopes in medical institutions. The two water samples were inoculated by pour plate and membrane filtration methods, respectively. Using statistical methods including Spearman and Pearson correlation, Wilcoxon signed-rank sum test, paired-Chi-square test, and linear regression, we analyze the differences and associations in the bacterial counts cultivated through two different methods.ResultsIn 142 specimens of the water, the median and interquartile range of the heterotrophic bacterial colony number under the R2A culture method and under the PCA culture method were 200 (Q1–Q3: 25–18,000) and 6 (Q1–Q3: 0–3700). The total number of heterotrophic bacteria colonies cultured in R2A medium for 7 days was more than that cultured in PCA medium for 2 days (P < 0.05). The linear regression results showed a relatively strong linear correlation between the number of colonies cultured by the R2A method and that cultured by the PCA method (R2 = 0.7264). The number of bacterial species detected on R2A agar medium is greater than that on PCA agar medium.ConclusionThe R2A culture method can better reflect the actual number of heterotrophic bacterial colonies in hospital purified water. After logarithmic transformation, the number of colonies cultured by the two methods showed a linear correlation.

Perbedaan Jumlah Koloni Bakteri Subgingiva Setelah Menyikat Gigi dengan Berbagai Pasta Gigi yang Mengandung Bahan Herbal Sirih (Peper betle Linn.), Siwak (Salvadaro persica) dan Cengkeh (Eugenia caryophyllus) pada Anak dengan Disabilitas Intelektual

Children with Intellectual Disabilities have a higher risk of oral diseases such as dental caries and periodontal diseases compared to other children. Therefore, efforts are needed to help maximize the prevention of these diseases. The abundance of herbal materials in the community, which have been proven to have antibacterial effects, has been increasingly formulated into toothpaste. This is expected not only to provide a mechanical cleaning effect on plaque but also to have antibacterial effects and inhibit plaque growth for a longer period in the oral cavity.This research aims to determine the difference in subgingival bacterial colonies after brushing teeth with various herbal-containing toothpaste in children with intellectual disabilities. The study was conducted using a quasi-experimental pre-post control group design. Subgingival fluid samples were taken from respondents aged 6-12 years before and after brushing their teeth with four groups: (K) without toothpaste, (A) toothpaste containing betel leaves (Piper betle Linn.), (B) toothpaste containing Miswak (Salvadora persica), and (C) toothpaste containing Clove (Eugenia caryophyllus). The samples were then cultured in Plate Count Agar media for 48 hours. Colony counting was performed using the Total Plate Count (TPC) method with a Colony Counter. Statistical analysis was conducted using Wilcoxon and Kruskal-Wallis tests.The results of the study showed a decrease in the number of CFUs (colony-forming units) after brushing teeth. Significant reductions were obtained in toothpaste B (p-value = 0.046) and toothpaste C (p-value = 0.028). In conclusion, toothpaste containing Miswak and Clove effectively reduces the number of bacterial colonies formed in subgingival fluid samples..

THE EFFECTIVENESS OF LEVOFLOXACIN 0.5% WITH POVIDONE IODINE 2.5% IN REDUCING CONJUNCTIVA BACTERIAL FLORA IN PRE INTRA OCULAR OPERATIVE

Abstract&#x0D; Introduction &amp; Objectives : This study aims to compare the effectiveness of Levofloxacin ED 0.5% with Povidone iodine 2.5% in reducing normal bacterial flora of the conjunctiva in preoperative procedures.&#x0D; Methods : Clinical trials will be conducted in the inpatient ward on patients who will undergo intravitreal surgery at Mohammad Hoesin Hospital Palembang from May 2022-July 2022. Subjects were divided into two groups, which is Levofloxacin ED 0.5% and Povidone iodine 2.5% group. Before and after the fornices preparation, the number of bacterial colonies that grow will be counted and evaluated using bivariate analysis.&#x0D; Results : There were 32 samples that fulfilled the inclusion criteria. A significant decrease of conjunctival flora was observed in both groups (p value = 0.000). Comparison of the two groups’ decrease in conjunctival flora was not significant. There was a reduction of up to 100% in 11 samples (68.75%) of both intervention groups.&#x0D; Conclusion : The use of Levofloxacin 0.5% and Povidone iodine 2.5% was equally good in reducing the number of bacterial colonies in the conjunctiva.

Analysis Of Bacterial Contamination By Total Plate Number (ALT) Method In Smoked Fish At One Of The Market Traders

Bacterial contamination can come from the environment as a result of food production processes in the form of biological, chemical and foreign matter contaminants that can interfere with, harm and endanger human health. One of the problems in the processed food sector is the high level of bacterial contamination in the food served on the market. The condition of open food is also the main factor causing food to be contaminated with bacteria. The Total Plate Count (ALT) or total plate count (TPC) is an examination used to determine the number of microorganisms both bacteria and fungi using solid media with the final result being colonies. The aim of the study was to determine bacterial contamination and to determine the total plate number (ALT) of bacterial colonies in smoked fish sold at Ngemplak Market, Tulungagung. This research used a Simple Random Design (RAS) method with four treatments and 3 repetitions. The results showed that for menot fish it was 16.2 x 105 cfu/g, for tuna it was 10.5 x 105 cfu/g, for tuna it was 9.4 x 105 cfu/g, for bader fish it was 16.4 x 105 cfu/g. g, and in whitefish 20.5 x 105 cfu/g. The conclusion is that the bacterial contamination of smoked fish sold at the Ngemplak Market using the ALT method exceeds the normal limit value set by SNI 2725:2013.

Antibacterial Irrigation Solution Cocoa Bean Cuticle Extract (Theobroma cacao L) Against Streptococcus viridans

Background: Pulp necrosis is the demise of the pulp due to persistent infection in the pulp area, such as Streptococcus viridans. Irrigation steps during biomechanical preparation and sterilization play a very important role. The irrigation material often used is 2.5% NaOCl because it has a broad antibacterial spectrum but is irritating and has an unpleasant aroma. The cuticle of cocoa beans (Theobroma cacao L.) might be an alternative root canal irrigation material. The Objective: To determine the antibacterial properties of irrigation solution of cocoa bean cuticle extract against Streptococcus viridans. Materials and Methods: This study was laboratory experimental with a post-test-only control group design by calculating the number of bacterial colonies. Consisting of eight research groups, namely cocoa bean cuticle extract with concentrations of 25% (P1), 12.5% (P2), 6.25% (P3), 3.12% (P4), 1.56% (P6), 0.78% (P6), NaOCl 2.5% (K+), and sterile distilled water. 0.5 ml of each group was taken and then mixed with 0.5 suspension of Streptococcus viridans, diluted 10-4. Next, the extract and bacterial suspension mixture 10-4 were incubated for 24 hours, then 0.1 ml was inoculated on BHIA media and incubated. After that, the colony counter manually counted the number of colonies. Results: The result showed a significance of p&lt;0.5, followed by the Mann-Whitney test, which showed significant differences except in group P1 (25%) with K+ (NaOCl 2.5%). Conclusion: Cocoa bean cuticle extract irrigation solutions with concentrations of 25%, 12.5%, 6.25%, 3.12%, 1.56% and 0.78% presented antibacterial power against Streptococcus viridans. Keywords: Streptococcus viridans, Antibacterial, Cocoa bean epidermis extract, Irrigation solution.

The Use of (O. Basilicum) Extract to Treat Litopenaeus vannamei Disease the Infected Bacteria Vibrio parahaemolyticus

This research aims to treat vaname shrimp disease infected with bacteriaVibrio parahaemolyticus. In this research, we used Completely Randomized Design (CRD) 4 treatments and 3 replications. The treatment being tested was the treatment of vaname shrimp disease by soaking in basil (O. basilicum) leaf extract with different concentrations, namely: Treatment A (0 mg/ml), treatment B (1.56 mg/ml), treatment C (3.12 mg/ml) and treatment D (12.5 mg/ml). The parameter observed was the number of bacterial coloniesV. parahaemolyticus maintenance media and in the hepatopancreas of vaname shrimp. Apart from that, observations were made on the survival rate of vaname shrimp and water quality parameters. The research results found that the best concentration in the treatment of diseaseV. parahaemolyticus in vaname shrimp at a concentration of 12.5 mg/ml with the lowest number of bacterial colonies in the rearing media, namely 1.20 x 102 CFU/ml. In the hepatopancreas of vaname shrimp, the lowest number of colonies was obtained at a treatment concentration of 12.5 mg/ml, namely 1.12 x 102 CFU/ml. The highest survival rate was obtained at a treatment concentration of 12.5 mg/ml of 86,67±5,77%. The range of water quality in research observations was obtained as follows salinity 28.72-29.05 ppt, temperature 26.71- 27.25OC, oxygen ranges from 5.72-7.81, pH 7.18-8.13 and ammonia 0.033-0.041ppm. Water quality during the research was in the optimal range for the life of vaname shrimp.

Comparison of chlorine dioxide and chlorhexidine 2% antiseptic in reducing bacterial colony counts as an alternative to DUWLs cleaning: a quasi-experimental study

ABSTRACTIntroduction: The inner surfaces of dental unit waterlines (DUWLs) possess an accumulation of any bacteria. Microorganisms can enter dental unit waterlines from water reservoirs. Antiseptics are substances that inhibit the growth of bacteria. Chlorine dioxide is effective in decontaminating microbes in the DUWLs and has a beneficial effect on reducing nosocomial infections. Chlorhexidine effectively prevents the growth of Streptococcus bacteria. The addition of antiseptic agents to the water source contributed to a significant reduction of the cultivable microbial counts in the aerosol. The purpose of this study was to analyze the difference between Chlorine Dioxide 0,1% and Chlorhexidine 2% antiseptics on the number of bacterial colonies in the Dental Unit Waterline. Methods: This study was a quasi-experimental study with a total sample size of 8 dental units that have water tanks in the oral surgery clinic. The sampling technique was total sampling, where the sample was divided into 2 groups, group I using Chlorine Dioxide and group II using 2% Chlorhexidine calculation of colony counts unit using the plate count method. Results: The difference in the number of colonies before being given Chlorine Dioxide was 13,153 CFU/mL and after being given Chlorine Dioxide antiseptic was 6,070 CFU/mL, while before being given 2% Chlorhexidine antiseptic was 12,917 CFU/mL and after being given 2% Chlorhexidine antiseptic was 2,823 CFU/mL. There is a significant difference in the number of bacterial colonies before and after being given Chlorine Dioxide and Chlorhexidine 2% antiseptic with ρ=0.001; Conclusion: Chlorhexidine 2% reduces bacterial colony forming unit in DUWLs much more than using Chlorine dioxide for the alternative of DUWLs Cleaning Agents.Keywords: DUWLs, chlorine dioxide, chlorhexidine 2%, bacterial colony counts

Identification and Counting of Bacterial Colonies on Mobile Phones Before and After 70% Alcohol Disinfection

Cell phones have become a very important communication tool in everyday life. Considering that many people are in the habit of using their mobile phones while doing other activities, most people even have the habit of taking their phones to the toilet. Cell phones become inanimate objects that play an important role in the transmission of microorganisms. Therefore, we need to maintain hand hygiene by keeping our hands clean after handling the mobile phone before performing activities and disinfecting the mobile phone regularly, considering that this object cannot be washed while the mobile phone is a potential vector for infectious diseases. Mobile phones can effectively eliminate hand hygiene due to their compulsive use despite hand washing. The purpose of this study was to determine the role of 70% alcohol cotton on the surface of mobile phones to reduce the potential spread of bacteria. Based on the results of the research conducted, there is a difference in the number of bacterial colonies on mobile phones before and after disinfection with 70% alcohol cotton, as evidenced by the obtained p-value of 0.0001 (p &lt;0.05).

Effect of medicine food homology Penthorum chinense Pursh on physicochemical property and volatile flavor substances analysis of Chinese sausage

Abstract Due to the adverse effects of synthetic chemical antioxidants, natural plant extracts, such as Penthorum chinense Pursh extract, have been demonstrated to possess antioxidative properties in Chinese sausages. Nevertheless, some physicochemical properties remain obscure. This study sought to determine the direct relationships between changes in pH, moisture, water activity, total viable count, color traits, volatile flavor compounds, and sausage quality. Therefore, P. chinense Pursh extract (PCPE) were added to Chinese sausage instead of synthetic chemical antioxidants. The results proved that the PCPE could reduce the a w value and stabilize the pH and moisture. The total number of bacterial colonies in the 0.1 % and 0.3 % sample groups was 7 % lower than that in the blank group on the 35th day. The PCPE could also maintain an acceptable appearance in Chinese sausage; the 0.3 % PCPE group presented a better final a* value, and the 0.1 % PCPE group had a better b* value. Additionally, eight volatile flavor compounds (ROAV &gt; 0.1), contributing to the flavor of Chinese sausage, were identified in the 0.3 % PCPE group by the ROAV analysis. Overall, the P. chinense Pursh extract could be a potential alternative to synthetic chemical antioxidants in the production of Chinese sausages.

Antibacterial and Phytochemical Properties of Clove Extracts on Vegetable Spoilage Bacteria

The present study was carried out to analyze the antibacterial and phytochemical properties of clove extracts on vegetable spoilage bacteria. The study seeks to determine its potential use as bio-preservative. The bacteria used for this study were isolated from infected leaves of bitter leaf, pumpkin and cocoyam using spread plate technique. Phytochemical analysis was carried out with aqueous and methanol extracts. Bacterial isolates from the leaves included Bacillus spp., Micrococcus spp., Staphylococcus spp. and Actinomyces spp. The pumpkin leaf had the highest average number of bacterial colonies of 2.5 x 102 while cocoyam leaf had the least with 7.0 x 101.Actinomycetes sp2 had the highest zone of inhibition of 22.0 mm while Staphylococcus sp2 had the least zone of inhibition of 13.00 mm all at 500mg/ml. For aqueous extracts, Bacillus sp.3, Staphylococcus sp.1 had the highest zone of inhibition of 16.00 mm respectively while the least zones were observed in Staphylococcus sp.2 and Bacillus sp.3 at concentration of 250 mg/ml and 125 mg/ml respectively. Bacillus sp.1, Bacillus sp.2, Micrococcus sp. and Actinomyces sp.1 has Minimum Inhibition Concentration (MIC) of 125 mg/ml while Staphylococcus sp.1 and Staphylococcus sp.2 has 250mg/ml respectively for methanol extract. For aqueous extract, Bacillus sp.2, Micrococcus sp. and Actinomyces sp.1 has MIC of 250mg/ml, respectively and Staphylococcus sp.1 and Staphylococcus sp.2 has MIC of 500 mg/ml. The Minimum Bactericidal Concentration (MBC) for the methanol extracts was 125 mg/ml for Actinomyces sp.1 while Bacillus sp.2, Bacillus sp.3, Micrococcus sp. and Actinomyces sp.2 were 250 mg/ml respectively while Bacillus sp1, Bacillus sp2, Micrococcus sp., Staphylococcus sp.2 and Actinomyces sp.1 all has MBC of 500mg/ml, respectively on the aqueous extracts. The phytochemical analysis of the aqueous and methanol extracts of clove shows that saponins, tannins, amino acids, proteins, reducing sugar, glycosides, alkaloids, anthraquinone and phenols are present though in varying amounts. This study therefore recommends clove extract as an alternative source for bio-preservation in food and allied products.

Mildew detection in rice grains based on computer vision and the YOLO convolutional neural network.

At present, detection methods for rice microbial indicators are usually based on microbial culture or sensory detection methods, which are time-consuming or require expertise and thus cannot meet the needs of on-site rice testing when the rice is taken out of storage or traded. In order to develop a fast and non-destructive method for detecting rice mildew, in this paper, micro-computer vision technology is used to collect images of mildewed rice samples from 9 image locations. Then, a YOLO-V5 convolutional neural network model is used to detect moldy areas of rice, and the mold coverage area is estimated. The relationship between the moldy areas and the total number of bacterial colonies in the image is obtained. The results show that the precision and the recall of the established YOLO-v5 model in identifying the mildewed areas of rice in the validation set were 82.1% and 86.5%, respectively. Based on the mean mildewed area identified by the YOLO-v5 model, the precision and recall for light mold detection were 100% and 95.3%, respectively. The proposed method based on micro-computer vision and the YOLO convolutional neural network can be applied to the rapid detection of mildew in rice taken out of storage or traded.