Number Of Somatic Cells Research Articles

Scanning Electrochemical Microscopy-Somatic Cell Count as a Method for Diagnosis of Bovine Mastitis.

Simple SummaryMastitis is inflammation/swelling in the breast, which is generally caused by an infection. In this study, we present scanning electrochemical microscopy-somatic cell count (SECM-SCC) as a novel method for diagnosis of mastitis in bovines. We developed a biosensor in this study that can serve as a highly promising portable electrochemical device for mastitis diagnosis in bovines.The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 105 cells/mL or more, the condition is referred to as mastitis. In the current study, we created a milk cell chip that serves as an electrochemical method that can be easily produced and used utilizing scanning electrochemical microscopy (SECM). The microelectrode present in the cell chip scans, and the difference between the oxygen concentration near the milk cell chip and in bulk is measured as the oxygen (O2) reduction current. We estimated the relationship between respiratory activity and the number of somatic cells in raw milk as a calibration curve, using scanning electrochemical microscopy-somatic cell count (SECM-SCC). As a result, a clear correlation was shown in the range of 104 cells/mL to 106 cells/mL. The respiration rate (F) was estimated to be about 10–16 mol/s per somatic cell. We also followed the increase in oxygen consumption during the respiratory burst using differentiation inducer phorbol 12-myristate 13-acetate (PMA) as an early stage of mastitis, accompanied with an increase in immune cells, which showed similar results. In addition, we were able to discriminate between cattle with mastitis and without mastitis.

Using of DNA-testing of cattle for the LTF and MBL1 genes to increase the milk production efficiency

In cows of the Belarusian black-motley breed, the polymorphism of genes for lactoferrin and mannose-binding leсtin was established. The genotypes LTFAA, LTFAB and MBL1TT, MBL1TC, MBL1CC were identified. The milk productivity (yield, fat content, amount of milk of basic fat content), the number of somatic cells in milk were studied, and the economic efficiency of milk production from cows with different genotypes for the genes of lactoferrin and mannose-binding leсtin was calculated. It was found that the profit in cows with the LTFAA genotype was 7.91 rubles higher than in cows with the LTFAB genotype, in individuals with the MBL1CC and MBL1TT genotypes, and a higher profit per head was obtained from cows with the LTFABMBL1CC and LTFAAMBL1TC genotypes, which amounted to 3249.49 and 2855.40 rubles respectively.

Effects of Saccharomyces Cerevisiae Cultures on Performance and Immune Performance of Dairy Cows During Heat Stress.

The dairy farming industry is facing massive economic losses as heat stress continues to rise. The purpose of this study was to see how feeding Saccharomyces cerevisiae culture (SC) influences productive performance, lactation performance, serum biochemical indexes, hormonal level, antioxidant capacity, and immune function in mid-lactating cows during heat stress. Forty-five healthy mid-lactation dairy cows with comparable milk yield, lactation days, and parity were randomly divided into 3 groups (15 cows in each group). The control group (CON) was fed the basal diet, while the treatment groups were fed the basal diet + first Saccharomyces cerevisiae culture 100 g/d (SC-1) and the basal diet + second Saccharomyces cerevisiae culture 30 g/d (SC-2), respectively. The SC-1 and SC-2 groups with SC added in the treatment groups reduced rectal temperature and respiratory rate in heat-stressed cows (P < 0.05). The milk yield of SC-1 and SC-2 treatment groups was significantly higher than that of CON (P < 0.05). Except for somatic cell count, which was significantly lower in SC-1 and SC-2 than in CON (P < 0.05), there were no significant differences in the milk components. The addition of SC: (i) increased serum urea levels (P < 0.05), but there was no significant difference in glucose, total cholesterol, alanine transaminase, aspartate aminotransferase, total protein, albumin and alkaline phosphatase levels (P > 0.05); (ii) increased serum levels of immunoglobulin-A, immunoglobulin-G, immunoglobulin M, interleukin-4, interleukin-10 and heat shock protein-70 (P < 0.05), while decreasing serum levels of interleukin-1β, interleukin-6, interleukin-2, interferon-γ and tumor necrosis factor-α (P < 0.05); (iii) increased total antioxidant capacity, glutathione peroxidase and superoxide dismutase in serum (P < 0.05), while decreasing malondialdehyde; (iv) increased serum levels of glucocorticoids, insulin, cortisol and prolactin (P < 0.05), while decreasing the serum levels of triiodothyronine and thyroxine (P < 0.05). In conclusion, under the current experimental conditions, the addition of SC can reduce rectal temperature and respiratory rate in heat-stressed mid-lactation cows, reduce the number of somatic cells in milk and improve the mid-lactation cow performance. In addition, SC addition to the diet can raise serum urea levels, regulate serum hormone levels, boost antioxidant capacity in mid-lactation cows, and boost overall immunity.

The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production.

Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (ASMT)-overexpressed melatonin-enriched dairy goats were successfully generated through the use of pBC1-ASMT expression vector construction and prokaryotic embryo microinjection. Results: These transgenic goats have the same normal phenotype as the wild-type goats (WT). However, the melatonin levels in their blood and milk were significantly increased (p < 0.05). In addition, the quality of their milk was also improved, showing elevated protein content and a reduced somatic cell number compared to the WT goats. No significant changes were detected in the intestinal microbiota patterns between groups. When the animals were challenged by the intravenous injection of E. coli, the ASMT-overexpressed goats had a lower level of pro-inflammatory cytokines and higher anti-inflammatory cytokines compared to the WT goats. Metabolic analysis uncovered a unique arachidonic acid metabolism pattern in transgenic goats. Conclusions: The increased melatonin production due to ASMT overexpression in the transgenic goats may have contributed to their improved milk quality and enhanced the anti-inflammatory ability compared to the WT goats.

Correlations between the Total Antioxidant Activity and Biochemical Parameters of Cow Milk Depending on the Number of Somatic Cells.

The aim of the work was to study the correlations between the total amount of water-soluble antioxidants (TAWSA) and biochemical parameters (BC) of cow milk depending on the somatic cell count (SCC). The BC and TAWSA values of cow milk were measured by spectroscopic and amperometric methods, respectively. The milk samples from the black-and-white cows (Moscow region) were divided according to SCС values: (1) ≤200, (2) 200-499, (3) 500-999, and (4) ≥1000 thousand units/mL. The average TAWSA values for groups 1, 2, 3, and 4 (33, 15, 13, and 12 milk samples) were the following: 15.95 ± 0.74, 14.45 ± 0.84, 16.04 ± 0.63, and 14.58 ± 1.18. The correlations between TAWSA and BC (group 1) were the following: total fat percentage (TFP) -0.305; true protein percentage (TP1) -0.197; total nitrogen percentage (TN2) -0.210; lactose -0.156; solids-not-fat (SNF) -0.276; total dry matter (TDM) -0.399; freezing point (FP) -0.112; pH -0.114; somatic cell count (SCC) - (-0,052). The correlations between TAWSA and BC (group 2) were the following: TFP -0.332; TP1 -0.296; TN2 -0.303; lactose - (-0.308); SNF -0.159; TDM -0.391; FP -0.226; pH - (-0.211); SCC -0.193. The correlations between TAWSA and BC (group 3) were the following: TFP - (-0.352); TP1 - (-0.411); TN2 – (-0.401); lactose - (-0.166); SNF - (-0.462); TDM - (-0.504); FP - (-0.766); pH - (-0.047); SCC - (-0.698). The correlations between TAWSA and BC (group 4) were the following: TFP -0.159; TP1 -0.046; TN2 – 0.077; lactose - (-0.317); SNF - (-0.237); TDM -0.058; FP - (-0.036); pH - (-0.477); SCC - (-0.072). These data are important in assessing the physiological-biochemical status and state of the antioxidant defense system of cows' organism.

Study of the resistance of cows with mastitis depending on the age of lactation

Relevance.The breeding stock and young cattle in the conditions of industrial technology of animal husbandry experience an increased antigenic load, which leads to disturbances in the immune status and the development of inflammatory processes manifested by endometritis and mastitis, diarrheal and respiratory syndromes. It was relevant to conduct a comprehensive assessment of the immunological reactivity of the animal’s body according to the state of resistance in cattle with clinical and subclinical mastitis, depending on the age of lactation. As a result of complex studies, the specific and nonspecific reactivity of the body of cattle in different periods of lactation was studied.Methods.The object of the study were cows of a black-and-white Holstein breed (n = 450). Groups of animals were formed according to the age of lactation:1st lactation, 2–3rd lactation, 3–4th lactation and for health reasons: clinically healthy animals, with subclinical mastitis and clinical mastitis. Milk samples were taken from cows to count the number of somatic cells, and blood to determine the quantitative content of IgG immunoglobulin and indicators of nonspecific resistance.Results.It was found that with mastitis in cows of different lactation ages, almost identical changes in the immune status were observed. Quantitative determination of IgG levels in animal blood serum is the basis for assessing the immunity of cattle. The immune status in clinically healthy animals was characterized by a higher level of the total amount of IgG immunoglobulin in the blood serum (amounted to 22.25 ± 0.70 mg/ml), whereas in cows with a subclinical form of mastitis, the level of IgG immunoglobulin decreased by 12.3%, with a clinical form of mastitis — by 17.2%. The results also showed that in cows of different lactations with subclinical and clinical mastitis, the bactericidal activity (by 10.8%) and the lysozyme activity of the blood increased significantly (by 8.22%). In cows with clinical mastitis — by 8.6% and 9.7% respectively, compared with the indicators in groups of healthy animals. Thus, mastitis, occurring in acute and subacute form, leads to a decrease in the resistance of the cows’ organism.

Novel anti-aging herbal formulation Jing Si displays pleiotropic effects against aging associated disorders

Common characteristics of aging include reduced somatic stem cell number, susceptibility to cardiac injuries, metabolic imbalances and increased risk for oncogenesis. In this study, Pleiotropic anti-aging effects of a decoction Jing Si herbal drink (JS) containing eight Traditional Chinese Medicine based herbs, with known effects against aging related disorders was evaluated. Adipose derived mesenchymal stem cells (ADMSCs) from 16 week old adult and 24 month old aging WKY rats were evaluated for the age-related changes in stem cell homeostasis. Effects of JS on self-renewal, klotho and Telomerase Reverse Transcriptase expression DNA damage response were determined by immunofluorescence staining. The effects were confirmed in senescence induced human ADMSCs and in addition, the potential of JS to maintain telomere length was evaluated by qPCR analysis in ADMSCs challenged for long term with doxorubicin. Further, the effects of JS on doxorubicin-induced hypertrophic effect and DNA damage in H9c2 cardiac cells; MPP+-induced damages in SH-SY5Y neuron cells were investigated. In addition, effects of JS in maintaining metabolic regulation, in terms of blood glucose regulation in type-II diabetes mice model, and their potential to suppress malignancy in different cancer cells were ascertained. The results show that JS maintains stem cell homeostasis and provides cytoprotection. In addition JS regulates blood glucose metabolism, enhances autophagic clearances in neurons and suppresses cancer growth and migration. The results show that JS acts on multiple targets and provides a cumulative protective effect against various age-associated disorders and therefore it is a candidate pleiotropic agent for healthy aging.

КОРРЕЛЯЦИОННЫЙ АНАЛИЗ ФАКТОРОВ, ВЛИЯЮЩИХ НА УРОВЕНЬ БАКТЕРИАЛЬНОЙ ОБСЕМЕНЕННОСТИ МОЛОКА

When analyzing raw milk properties, we should take into account the complexity and ambiguity of the “bacterial contamination” criterion. The authors evaluated the influence of physico-chemical and microbiological parameters of milk on its contamination over a year period. Milk testing protocols for 2020 were used as a source of information. The values of acidity, the number of mesophilic aerobic and facultative anaerobic microorganisms (CMAFAnM), the number of somatic cells, the proportion of protein and fat, and the density of milk were studied. To assess the influence of environmental parameters, the authors took ionto account the values of air temperature and humidity at the moment of sampling. Sampling was carried out according to GOST 26809.1-2014 “Milk and dairy products. Acceptance rules, sampling methods, and sample preparation for analysis”. The research was carried out on three dairy farms of the Knyaginino district of the Nizhny Novgorod region. They included a dairy farm with tie-up housing for 200 heads with milking in a linear type milk pipeline; a dairy farm with loose housing for 400 heads with milking in a herringbone type milk pipeline; a dairy farm with combined (tie-up and loose) housing for 450 heads with milking in a linear-type milk pipeline. The lowest indicator of QMAFAnM (total viable content) was noticed in March, and the highest – in November. The correlation analysis showed that the greatest influence of the studied factors on the level of bacterial contamination is exerted by the number of somatic cells (Pearson correlation coefficient R = 0.71 with a confidence probability P = 0.0619). The authors suppose that this situation may result from improper hygienic condition of the udder, equipment, and premises. There was a weak influence on the level of bacterial contamination of milk density (R = –0.18 at P = 0.096) and ambient air temperature (R = –0.18 at P = 0.095), which may be result from insufficient cooling of milk before transportation.

Indicators of full value feeding rations for dairy cows.

This article presents the results of the level of feeding and the health status of dairy cows in a commercial dairy farm on the content of the main components in milk (fat, protein, urea). The aim of the research was to study the milk productivity and composition of milk of cows, to analyze the level of feeding and the content of basic nutrients in the diet of the farm. The farm breeds purebred Holstein cattle with a high genetic potential for productivity. The milking herd was formed in 2009 on the basis of 600 heads of Holstein-Friesian heifers imported from Hungary, as well as 65 heads of Holsteinized heifers of Ukrainian selection from Ukraine in 2015. Today the livestock numbers about 1,500 head of cattle, of which there are about 900 breeders. On the territory of the farm there are: 3 cowsheds for keeping cows, an insemination room, a milking parlor with a parallel installation of the Delaval company, in which 48 cows are milked for one milking, the ABK, where the manager's office, livestock technician, accounting is located, and a mini-hotel with dining room and lounges. Dairy bases are equipped with auto-drinkers, ventilation, plumbing, electric lighting, manure removal mechanisms, and a milking installation. The object of the research was Holstein cows in the amount of 483 heads of Aina Dairy Farm LLP. The studies were carried out according to generally accepted zootechnical methods using modern equipment for conducting analyzes and interstate and state standards. The research results showed that the milk productivity of cows averaged 19.5 kg per day, the fat content 4.3%, the protein 3.9%, the number of somatic cells 230.5 thousand units / ml, urea 45.3 mg / 100 ml respectively. Analyzing the level of urea in milk can suggest a high crude protein content in the diet. The ration of feeding dairy cows on the farm is concentrate-silage-haylage and there is an excess of dry matter by 16.2%, crude protein by 9.8%, starch by 29.4%, respectively.

ВЕТЕРИНАРНО-САНИТАРНАЯ ОЦЕНКА КАЧЕСТВА МОЛОКА КОРОВ ПОСЛЕ ОБРАБОТКИ СОСКОВ ВЫМЕНИ ГИГИЕНИЧЕСКИМИ СРЕДСТВАМИ

Under supervision were 1,400 cows with a productivity of more than 13.0 tons of milk in 305 days of lactation, which were divided into 4 groups: the 1st experimental – hygienic products were not used; 2nd - animals treated nipples with a hygienic agent based on chlorhexidine bigluconate hydrochloride/aloe tree oil («CGB aloe»); 3rd - cows were treated with udder nipples with a disinfectant «Teasfoam Supercow»; 4th – the hygienic agent «ProfilacDryOff» was used. It was found that in cows after the use of «CGB aloe» before and after milking there is a pronounced positive correlation between the number of somatic cells and the concentration of lactoferin in milk (0,974... 0,993; p &gt; 0,999) and a moderate negative correlation between free oxyproline and lactoferin content and lactoperoxidase activity (0,477... 0,866; p &gt; 0.95). The absence of biochemical changes in the milk of cows allows us to conclude that the use of hygienic agents before and after milking does not negatively affect the composition of milk. There is an increase of lactoperoxidase activity by 1.42 times and a decrease of lactoferin concentration by 2.52 times compared to their content in milk of cows without application of hygienic agents. Based on the results obtained, the following conclusion can be made: disinfection of nipples by the hygienic agent «CGB aloe» before and after milking cows does not prevent hyperkeratosis of nipples in 2.4% of animals. In addition, 12.1% are diagnosed with subclinical mastitis. When disinfecting udder nipples with the Teasfoam Supercow agent, hyperkeratosis of udder nipples was noted in 3.3% of cows, 12.9% had subclinical mastitis.

EVALUATION OF MILK QUALITY PARAMETERS FROM COWS WITH MASTITIS

The social significance of mastitis is manifested in the fact that the causative agents of mastitis in cows can cause disease in humans. Since the leading role in mastitis pathology belongs to streptococci and staphylococci, it is they who most often enter the milk. The purpose of the research was to establish the frequency of occurrence of subclinical mastitis in cows, its effect on the physicochemical parameters of milk with the determination of the species composition of the microflora of milk in cows with subclinical mastitis with the development of a method for its diagnosis in the early stages of the development of inflammation of the mammary gland. The work is based on the results of analytical analysis of the literature, a comprehensive clinical, instrumental and laboratory study of lactating cows with mastitis. Studies have established that the disease of the udder in cows with mastitis at the beginning of lactation has a certain seasonal nature and its main peaks occur in March-May and September-November. During these months, from 2.5 to 4.1 % of cows with mastitis from the total number of the farm were identified. It has been established that with mastitis there is a change in the physicochemical parameters of milk: an increase in the number of somatic cells up to &gt;1500 thousand/cm3; an increase in the pH of the milk to 7.3, which is associated with the breakdown of milk proteins to ammonia, and an increase in the electrical conductivity of milk to 7.54 mS/sm, due to the entry of sodium and chlorine ions into milk from the blood. The task in developing a method for diagnosing mastitis in cows was to diagnose the disease at the early stages of the development of inflammation of the mammary gland and facilitate complex studies. This method consists in studying the composition of a biological fluid, which is applied to a glass slide in the form of a drop of milk from the examined quarter of the udder, dried to obtain a solid medium structure, and examined under a light microscope.

Mobiloscope: A Technological Solution for Early Mastitis Detection in Dairy Cattle

One of the most critical challenges in dairy farms is the Mastistis condition causing economic losses associated with milk production reduction and veterinary treatment expenses. Although it exists different methodologies for diagnosing animals with mastitis, these tests are usually indirect; others require laboratory analysis taking a lot of time to obtain the result, limiting its viability and monitoring in the field. To solve this problem, we propose a Mobiloscope, which is a portable, practical, effective, and low-cost diagnostic system for sub-clinical mastitis. Hence, this device provides an early detection in-situ and at a low cost to cover farmers' unsatisfied demand for having innovative tools that allow them to carry out better sub-clinical mastitis early detection. Our system comprises four components: (i) the holder for the electronic device and the screen to display the graphic interface; (ii) a part where the battery for the micro-computer will be housed; (iii) a dedicated part for the microscope and sample holder; and (iv) a holder for the light source. Despite the need to validate the prototype for commercial purposes, our prototype is able to estimate the number of somatic cells. Therefore, our mobiloscope could help the farmers to make an in-situ analysis of milk quality at a low-cost.

The Effect of Physically Effective Neutral Detergent Fiber on Milk Composition and Milk Yield

This study examines the effect of the physically effective Neutral Detergent Fiber (peNDF) value of roughage on milk composition and milk yield. The physically effective NDF varies depending on the particle length of the roughage and the amount of structural and non-structural carbohydrates in the diet. The quality, quantity, and particle length of the roughage are crucial for a healthy rumen. The longer the particle length of the roughage is, the more the cow ruminates and the more saliva it produces. The produced saliva is used to balance the rumen pH. It is aimed to protect rumen and animal health, improve milk and fertility, and make more efficient animal husbandry through the optimum preparation of physically effective NDF levels in the ration. The optimum NDF in the ration realizes the balance of energy, protein, and fiber required by cows in the early lactation period and makes it possible to maximize the microbial yield and protect the rumen's health. In this period, the lactation peak is maintained by promoting dry matter intake. The current study showed that the effect of peNDF on milk composition properties and somatic cell number significantly differed between farms (P

Modern view on the etiology, pathogenesis and diagnosis of mastitis in cows

Mastitis in cows is currently a pathology that causes great economic damage to livestock farms around the world. The quantitative and qualitative indicators of milk are decreasing. Prevention and treatment of breast inflammation in cows is one of the main tasks of veterinarians. For successful treatment, it is necessary to understand the etiology, especially in infectious mastitis. The degree of inflammatory reaction depends on the invading pathogen and the state of the animal's body, including such indicators as the stage of lactation, age, immune status, genetics and feeding diet. Almost 200 microorganisms that cause bovine mastitis have been identified, including bacteria, yeast, fungi and viruses. More than 150 species of bacteria that cause mastitis in cows have been identified, with bacterial mastitis being the most common. Mastitis can be caused by gram-positive bacteria, the most common of which are staphylococci and streptococci, and gram-negative bacteria, more often Escherichia coli and Klebsiella pneumoniae. Laboratory diagnostics is necessary to isolate and identify the relevant pathogen, which is necessary for the successful prevention and treatment of mastitis. The most commonly used method of diagnosing mastitis in cows is to measure the number of somatic cells. Express diagnostics are often used, which do not give a numerical result, but indicate only low or high indicators, while the causative agent of mastitis is not identified. The cultivation method is still the main criterion for detecting microorganisms, but it is a very time-consuming and expensive method. With the development of molecular methods, rapid and accurate diagnosis of diseases in animals has become possible. Recently developed diagnostic tests have shown high specificity and sensitivity.

Introduction of the Modern Methods of Assessing the Breeding Value of Cows in the Selection of Dairy Cattle in the Republic of Kazakhstan.

On the genetic improvement of animals, the need for decision-making takes place at the strategic, tactical, and operational levels. At the strategic level, this means defining a breeding goal, selecting a breeding system (selection or crossbreeding), as well as crossbreeding patterns, breeds, and lines. The current study aimed to analyze the breeding and genetic parameters of the dairy cattle in the Republic of Kazakhstan and introduce modern methods for assessing the breeding value of domestic and imported breeds of dairy cows. Research data were collected from primary zootechnical and breeding accounting (from the information and analytical system [IAS]), as well as experimental studies, visual assessment, measurements, and control milking of animals. In addition, biochemical studies of milk were conducted in this study. All animals were in the same conditions of feeding. The average milk yield per cow was 5,712±97 kg, with an average fat content of 3.83±0.02%, protein content of 3.28±0.01%, with the content of 339.6±54 thousand somatic cells. However, these data are obtained based on quarterly quality indicators of milk (fat content, protein, and the number of somatic cells), which raises doubts about the reliability of the results. It was found that the average index of the total estimated breeding value (EBV) for all breeds was 81. Among all breeds, the highest EBV was estimated at 84 in the Holstein cows (imported to the country).

Вимоги щодо створення умов для утримання тварин на молочних фермах

The purpose of the research is – to develop requirements for creating comfortable conditions for keeping farm animals, taking into account EU standards, during milk production on farms. Research methods. During the development of requirements for the creation of comfortable conditions for keeping farm animals, taking into account EU standards, in the process of milk production on farms used the following basic regulations: Council Directive 98/58/EU, Council Directive 91/629/EU, Council Directive 92/46/EU, Commission Directive 89/362/EC. Requirements in the field of milk production, adapted to EU standards, are systematized by the following components: keeping, feeding, watering animals, milking cows, milk cooling, manure removal, creating a microclimate, veterinary care. Research results. In practice, during milk production it is necessary to create free, comfortable conditions for keeping cattle. Convenient access of service personnel to the place of calving of cows should be provided. In the conditions of the maternity ward, the technological areas should be 8-10 m² per cow. Young cattle, including calves, should be provided with a clean and dry rest area, protected from drafts. According to EU regulations, calves up to eight weeks of age can be kept in individual cages or in group cages. According to EU Council Directive 97/2, calves older than 8 weeks of age are kept only in group cages. In accordance with the EU Directive 97/2 technological areas for keeping young cattle with a live weight of up to 150 kg - must be at least 1,5 m² / head., Live weight 150 - 220 kg – 1,7 m² / head., Live weight 220 kg and more – 1,8 m² / goal. Technological areas for keeping animals with a live weight of 200 to 500 kg - from 2,7 to 4,7 m² / goal. The feeding front for young cattle with a live weight of 200 to 500 kg is from 0,4 to 0,6 m / goal. The technological area in the section per cow must be at least 6 m² / head. Rational sizes of boxes for rest of cows: width 1,2 m, length (near a wall) - 2,6 m, length (in paired boxes) - 2,45 m, height of a protection of a box - 1,1 ± 0,05 m, an inclination boxing floor towards the manure passage 5 ± 1%, the height of the boxing floor above the level of the manure passage 0,2 – 0,25 m. The width of the rest area of cows must be at least 6 m. In accordance with EU Council Directive 98/58 must be provided free access of animals to feeders and feed. EU Commission Decision 97/182 states that priority should be given to the feeding of whole milk substitutes when feeding calves. A feeding front for cows must be provided – 0,7 ± 0,05 m. In accordance with EU Council Directive 98/58, free access of animals to drinking troughs and water must be ensured. According to European standards, the total bacterial contamination of milk should be - ≤ 300 thousand KUO / cm³, the number of somatic cells in milk - ≤ 400 thousand /cm³. Drainage of wastewater from milking parlors, household premises of the farm is carried out using a separate from the manure removal system - sewer system. The bactericidal phase of fresh milk is 4 hours, no later than this period you need to start processing milk into dairy products. The depth of the manure channel in the livestock building should be 8 - 20 cm, the sides of which are located at right angles to the surface of the channel. The minimum width of the manure passage for cows should be 2,7 m. An acceptable level of air temperature for cows during the year from minus 10 °C to + 25 °C at a relative humidity of up to 80 %. Periodically carry out preventive veterinary measures, weighing animals, trimming the hooves of limbs in cows 2-4 times a year, monthly examination of cows for mastitis using mastitis detectors. Conclusions. For the first time in Ukraine, requirements have been developed to create comfortable conditions for keeping farm animals, taking into account EU standards, during milk production on farms. Requirements adapted to EU standards in the field of milk production are systematized by the following components: keeping, feeding, watering dairy cattle, milking cows, cooling milk, manure removal, creating a microclimate, veterinary care.

Effect of temperature and relative humidity on the milk production of dairy cows

The aim of this study was to evaluate the influence of environmental housing conditions on the milk yield of dairy cows. Measurements were taken in the summer period from June to September 2020 and in the winter period during January 2021 on a large-capacity farm of Holstein Friesian cattle. Cows were housed in free stall barn with the lying boxes and selected during the second or third lactations, in the summer period from the 51st day to the 135th day and in the winter period from the 64th day to the 120th day of lactation. The average temperature in the housing was 23 °C in summer, and 7.05 °C in winter. The average THI (thermal humidity index) value in summer was 70.43, but during the day the THI values sometimes reached 75. The dairy cows were therefore exposed to heat stress during summer. Increasing THI and temperature values negatively affected the milk yield, as there was a negative correlation between both THI and milk yield (r = -0.641; p &lt;0.01) and temperature and milk yield (r = -0.637; p &lt;0.01). Milk production in winter was at 58.77 kg per day and in summer at 49.55 kg per day. In the summer, the milk had a significantly lower content of fat (p &lt;0.05), proteins (p &lt;0.001), lactose (p &lt;0.001), minerals (p &lt;0.001) and conversely, a higher number of somatic cells (p &lt;0.01). These results show that worse environmental conditions during the summer negatively affected the level of milk yield and the composition of the cows’ milk.

Application of bacillus megaterium for subclinical mastitis in cows

The problem of treatment of subclinical mastitis often arises after calving, especially in cows of the first lactation. The paper presents the results of the therapeutic effect of Bacillus megaterium in subclinical mastitis in cows.&#x0D; The aim of the research. To determine the effect of Bacillus megaterium on the microflora of the gastrointestinal tract and colonies of microorganisms isolated from subclinical mastitis in cows.&#x0D; Materials and methods. The research was conducted in a dairy farm growing Holstein. Cows with subclinical mastitis of the experimental groups were given concentrated feed with the addition of Bacillus megaterium (1 × 109 CFU/g) at a dose of 15–35 g per animal. The control group of cows was given the usual diet for dairy cows. The experiment lasted 30 days.&#x0D; Results. The use of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal had a pronounced effect on the microflora of the gastrointestinal tract of cows. The amount of Lactobacillus sp. was 67 % higher and Bifidobacterium 58 % higher than the control. In addition, the level of opportunistic pathogens on Escherichia coli decreased by 45 %, Clostridium by 27 %, Enterobacteriaceae and Staphylococcus by 75 %, and Candida by 80 % compared to controls. The amount of microflora in milk decreased by 40.2 % and the number of somatic cells by 87.9 %.&#x0D; Conclusions. The therapeutic efficacy of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal for 30 days in subclinical mastitis has been proven. After treatment, the amount of microflora in cow's milk decreased by 40.2 % and the number of somatic cells by 87.9 %.&#x0D; A positive effect on the microflora of the gastrointestinal tract of cows, where the number of Lactobacillus sp. increased by 67 % and Bifidobacterium by 58 %. The level of opportunistic pathogenic microflora decreased by Escherichia coli – by 45 %, Clostridium – by 27 %, Enterobacteriaceae and Staphylococcus – by 75 %, Candida – by 80 %, compared to the control.

Сравнительный анализ применения средств для обработки вымени в профилактике маститов и повышении качества молока коров

Abstract. The aim of this study is to study the effectiveness of the use of various means for treating the udder of cows in order to prevent the incidence of mastitis and improve the quality of milk. Since in order to prevent the development of mastitis for a long time, it is necessary not only to destroy the disease itself and fight its symptoms, but also to take all kinds of measures to prevent the disease. Methods. To conduct a scientific and economic experiment, 4 groups were formed, of which 3 are experimental and 1 control, according to the method of pairs-analogs using a tethered method of keeping in stalls in all four groups. To treat the udder, agents based on iodine, chlorhexidine, chlorhexidine digluconate and probiotic bacteria were used. Results. The summer use of Priolit Vortex and Algavit Vortex in 1 experimental group showed a decrease in the number of mesophilic aerobic facultative anaerobic microorganisms in cow's milk from 200,000 to 97,000 CFU/cm3, which is 48.5 % less than at the beginning of the experiment. Treatment with disinfectants Priolit Vortex and Elovit Vortex in the 2 nd experimental group made it possible to reduce the number of mesophilic aerobic facultative anaerobic microorganisms by 68.4 %. The use of probiotic agents Skin Cleaner and PIP Cow Teat Cleaner for treating the udder of cows allowed to reduce the number of mesophilic aerobic facultative anaerobic microorganisms in the milk of cows in experimental group 3 from 180,000 to 110,000 CFU / in 1 cm3, which is 61.1 % less than the initial indicators. Somatic cells in the milk of cows of the 1st experimental group decreased from 750,000 to 120,000 in 1 cm3, in the 2nd experimental group the number of somatic cells decreased from 250,000 to 180,000 in 1 cm3, in the 3rd experimental group from 390,000 to 340,000 in 1 cm3. In the control group, an increase in the number of somatic cells was found by 84 %.

MILK LIPIDS AND SUBCLINICAL MASTITIS

This article deals with the process of obtaining quality raw milk by analyzing its lipid composition. The lipid composition of raw milk depends on many factors, among which, first of all, is the species, the composition of the diet and the physiological state of the breast. In recent years, a large amount of data has accumulated on the fluctuations of certain lipid parameters of milk depending on the type, age, lactation, diet, time of year, exercise, animal husbandry technology, physiological state of the lactating organism in general and breast status in particular. Factors of regulation of fatty acid composition of raw milk: genetically determined parameters of quality and safety; fatty acid composition of the diet; synthesis of fatty acids by microorganisms of the digestive tract; synthesis of fatty acids in the breast; physiological state of the breast. The milk of each species of productive animals has its own specific lipid profile and is used in the formulation of certain dairy products to obtain the planned technological and nutritional parameters. Diagnosis of productive animals for subclinical mastitis involves the use of auxiliary (thermometry, thermography, electrical conductivity) and laboratory research methods: counting the number of somatic cells; use of specialized tests; microbiological studies of milk; biochemical studies of milk. The biochemical component in the diagnosis of subclinical forms of mastitis is underestimated. An increase in body temperature implies an increase in the intensity of heat release during the oxidation of substrates, sometimes due to a decrease in the intensity of synthesis of energy-intensive compounds. There are simply no other sources of energy in the body. The situation is the same with certain parts of the metabolism, which are aimed at the development of protective reactions to the etiological factor aimed at the defeat of the breast. That is why the biochemical composition of breast secretions in the absence of clinical signs of mastitis undergoes biochemical changes and the task of scientists is to develop mechanisms for clear tracking of such changes, identification of animals with subclinical forms of mastitis and effective treatment.