Nucleus Preopticus Research Articles

Pregnenolone, Pregnenolone Sulfate, and Cytochrome P450 Side-Chain Cleavage Enzyme in the Amphibian Brain and Their Seasonal Changes

To clarify whether the amphibian brain synthesizes de novo neurosteroids, we examined pregnenolone, pregnenolone sulfate ester, and cytochrome P450 side-chain cleavage enzyme (cytochrome P450scc), an enzyme converting cholesterol to pregnenolone, using amphibians. Pregnenolone and its sulfate ester in the brain, gonad, and plasma of Xenopus laevis were measured by a specific pregnenolone RIA. The concentrations of these two steroids in the female brain were significantly larger than those in the ovary and plasma. A similar tendency was evident in the male. In both sexes, pregnenolone and its sulfate ester were concentrated more highly in the cerebellum than in the telencephalon, diencephalon, or midbrain. An immunoreactive protein band of electrophoretic mobility in the proximity of bovine adrenal P450scc was detected in the Xenopus brain as well as the testis by Western blot analysis. Immunohistochemical analysis indicated that Purkinje cells in the Xenopus cerebellum were specifically immunostained with the P450scc antibody. P450scc-like immunoreactive cells were further found in several telencephalic and diencephalic regions, such as the pallium mediale and nucleus preopticus, in the Xenopus brain. A similar localization of P450scc-like immunoreactive cells was evident in Rana nigromaculata, a seasonally breeding amphibian. In the present study, seasonal changes in pregnenolone and its sulfate ester were further examined as a possible physiological change using R. nigromaculata. In both sexes, pregnenolone concentrations in the brain were almost constant during the seasonally breeding cycle. In contrast, the pregnenolone sulfate concentration in the brain was significantly lower in the hibernating quiescent phase and higher in the breeding and postbreeding active phases, independent of the plasma steroid level. These results taken together suggest that the amphibian brain possesses steroidogenic enzyme P450scc and produces pregnenolone and its sulfate ester. Pregnenolone sulfate may function well during the breeding and postbreeding active phases of the year in the seasonal breeder.

Immunocytochemical Study on the Localization and Distribution of the Somatolactin Cells in the Pituitary Gland and the Brain ofOreochromis niloticus(Teleostei, Cichlidae)

Using specific antibody for chum salmon somatolactin (SL), immunocytochemical studies were employed to determine the distribution of this hormone in the pituitary gland and the brain ofOrechromis niloticus.The results indicated that the SL-immunoreactive (ir) cells are found in the pars intermedia (PI) of the pituitary gland. The SL-ir cells showed strong and specific immunoreactivity to anti-chum salmon SL. Moreover, SL-ir cells were found to be widely distributed in most brain regions. Most of the SL-ir cell bodies were scattered along a nearly continuous line extending posteriorly from the olfactory bulb to the medulla oblongata through the nucleus preopticus periventricularis, habenula, and midbrain tegmentum and ventral to the nucleus lateralis tuberis pars posterior through the nucleus preopticus basalis lateralis and organum vasculosum luminae terminalis. Also SL-ir cells were observed in the cerebellum. The synthetic and secretory activity of the SL-ir cells, in the pituitary and the brain, showed an increase during sexual maturation and spawning. The highly organized SL-containing system and the gradual stimulation of SL synthesis and release during sexual maturation and spawning ofO. nilotcussuggest that SL may be involved in the control of some steps of reproductive processes.

Thyrotropin-releasing hormone concentrations in different regions of the chicken brain and pituitary: an ontogenetic study

The regional distribution of thyrotropin-releasing hormone (TRH) was studied in the chicken brain. The hypothalamus and the brain stem contained the highest concentration of TRH. Lower amounts were present in the telencephalon, the optic lobes and the cerebellum. Within the hypothalamus, TRH was most abundant in the median eminence. Other important TRH sites were the nucleus paraventricularis magnocellularis, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami and nucleus preopticus periventricularis. On the 14th day of embryonic development (E14), TRH was mostly found in the brain stem. Towards hatching, TRH concentrations increased gradually in both the hypothalamic area and the brain stem. TRH concentrations in the telencephalon, optic lobes and cerebellum remained low. Pituitaries from E14 to E16 chickens were characterized by a high TRH concentration, whereas hypophyseal TRH concentrations dropped towards hatching. Our results support the hypothesis that TRH exerts both endocrine and neurocrine actions in the chicken. On the other hand, high pituitary TRH concentrations were present when hypothalamic concentrations were low and vice versa. Therefore, the chicken pituitary may function as an important source of TRH during early in ovo development at least until the moment hypothalamic control develops.

Localization of mRNA Encoding Thyrotropin-Releasing Hormone Precursor in the Brain of Sockeye Salmon

Abstract The distribution of mRNA encoding thyrotropin-releasing hormone (TRH) precursor in the brain of sockeye salmon was studied by in situ hybridization histochemistry using digoxigenin-labeled riboprobes as a basis to investigate its physiological functions in the salmon brain. Since seasonal variation in TRH gene expression was expected in relation to smolting or maturation, fish were sampled in February and October. In both groups, TRH mRNA was widely distributed in discrete brain regions including the internal cellular layer (ICL) of the olfactory bulb, postcommissural nucleus of area ventralis telencephali (Vp), nucleus preopticus parvocellularis anterioris (PPa), nucleus preopticus magnocellularis, dorsal zone of periventricular hypothalamus (Hd), torus semicircularis, and also the motor nucleus of vagus nerve in the medulla oblongata. TRH neurons in ICL and Hd are round and small with diameters of 5–10 μm. In contrast, TRH neurons in the ventral telencephalon and the preoptic area are medium-si...

Appetitive and Consummatory Male Sexual Behavior in Japanese Quail Are Differentially Regulated by Subregions of the Preoptic Medial Nucleus

Central testosterone aromatization is required for the activation of both appetitive (ASB) and consummatory (CSB) male sexual behavior in Japanese quail. There are two major clusters of aromatase immunoreactive (ARO-ir) cells in the rostral forebrain; these outline the nucleus preopticus medialis (POM) and the nucleus striae terminalis (BST). We investigated the role of these nuclei in the regulation of ASB and CSB. Appetitive male sexual behavior was measured with the use of a learned social proximity procedure that quantified the time spent by a male in front of a window with a view of a female who was subsequently released into the cage, providing an opportunity for CSB. Males first acquired the response and then received bilateral electrolytic lesions aimed at the POM or BST, followed by retesting for ASB and CSB. Brain sections were stained for ARO-ir, and lesions to the two ARO-ir cell groups were quantitatively characterized. Lesions damaging the POM completely abolished CSB and also significantly decreased ASB. Lesions of the rostral BST had no effect on ASB, but moderately decreased CSB. Detailed anatomical analysis revealed that lesions of a subdivision of the POM just rostral to the anterior commissure specifically impair CSB, whereas lesions that are more rostral to this subdivision induce a severe deficit in ASB. These data indicate that different subregions of the POM regulate ASB and CSB in a somewhat independent manner, whereas the BST is only important in the regulation of CSB.

Anatomical and neurochemical definition of the nucleus of the stria terminalis in japanese quail (Coturnix japonica)

This study in birds provides anatomical, immunohistochemical, and hodological data on a prosencephalic region in which the nomenclature is still a matter of discussion. In quail, this region is located just dorsal to the anterior commissure and extends from the level of the medial part of the preoptic area at its most rostral end to the caudal aspects of the nucleus preopticus medialis. At this caudal level, it reaches its maximal elongation and extends from the ventral tip of the lateral ventricles to the dorsolateral aspects of the paraventricular nucleus. This area contains aromatase-immunoreactive cells and a sexually dimorphic population of small, vasotocinergic neurons. The Nissl staining of adjacent sections revealed the presence of a cluster of intensely stained cells outlining the same region delineated by the vasotocin-immunoreactive structures. Cytoarchitectonic, immunohistochemical, and in situ hybridization data support the notion that this area is similar and is probably homologous to the medial part of the nucleus of the stria terminalis of the mammalian brain. The present data provide a clear definition of this nucleus in quail: They show for the first time the presence of sexually dimorphic vasotocinergic neurons in this region of the quail brain and provide the first detailed description of this region in an avian species.

Anatomical and neurochemical definition of the nucleus of the stria terminalis in japanese quail (Coturnix japonica)

This study in birds provides anatomical, immunohistochemical, and hodological data on a prosencephalic region in which the nomenclature is still a matter of discussion. In quail, this region is located just dorsal to the anterior commissure and extends from the level of the medial part of the preoptic area at its most rostral end to the caudal aspects of the nucleus preopticus medialis. At this caudal level, it reaches its maximal elongation and extends from the ventral tip of the lateral ventricles to the dorsolateral aspects of the paraventricular nucleus. This area contains aromatase-immunoreactive cells and a sexually dimorphic population of small, vasotocinergic neurons. The Nissl staining of adjacent sections revealed the presence of a cluster of intensely stained cells outlining the same region delineated by the vasotocin-immunoreactive structures. Cytoarchitectonic, immunohistochemical, and in situ hybridization data support the notion that this area is similar and is probably homologous to the medial part of the nucleus of the stria terminalis of the mammalian brain. The present data provide a clear definition of this nucleus in quail: They show for the first time the presence of sexually dimorphic vasotocinergic neurons in this region of the quail brain and provide the first detailed description of this region in an avian species. J. Comp. Neurol. 396:141–157, 1998. © 1998 Wiley-Liss, Inc.

Distribution and effects of testosterone on aromatase mRNA in the quail forebrain: A non-radioactive in situ hybridization study

A number of studies have been devoted to the analysis of the anatomical distribution, control by steroids and functional significance of aromatase (the enzyme metabolizing testosterone into 17 β-estradiol) in the quail brain. In particular, the sexually dimorphic nucleus preopticus medialis has been the main focus of investigation because testosterone aromatization in this structure mediates the activation of male sexual behavior and aromatase activity is itself testosterone-dependent in this nucleus. No information on the anatomical distribution of aromatase gene expression is, however, available so far in this avian species. In the present study we applied a non-radioactive in situ hybridization technique to describe the distribution of aromatase mRNA containing neurons in the quail prosencephalon. We also analyzed, at a neuronal level of resolution, the induction by testosterone of this mRNA in the medial preoptic nucleus. Dense clusters of aromatase gene expressing neurons were observed within the medial preoptic nucleus, the nucleus of the stria terminalis, the ventro-medial hypothalamus and the tuberal region. Scattered neurons expressing lower levels of aromatase mRNA were also found in the dorsal thalamic area and central gray. The specificity of the staining was confirmed by demonstrating the absence of signal in sections that had been hybridized with a sense probe. Moreover, the distribution of the aromatase mRNA containing cells completely overlapped with the distribution of the aromatase-immunoreactive cells. Aromatase-mRNA expression was controlled by testosterone (or its metabolites) in the entire medial preoptic nucleus. Castration resulted in a decrease in the number of aromatase mRNA-containing cells and this effect was totally reversed by testosterone treatment. These data further support the idea that testosterone regulates the rate of its own aromatization by modulating the expression of aromatase rather than by acting at a post transcriptional level.

Immunohistochemical demonstration of oxytocin-like, neuropeptide Y-like and gonadotropin-releasing hormone-like substances in the hypothalamo-hypophysial system of ayu,plecoglossus altivelis altivelis, in osmotically different environments

To ascertain the role of neuropeptides on the hypothalamo-hypophysial system of a fish in osmotically different environments, an immunohistochemical study of oxytocin (OXT), neuropeptide Y (NPY) and gonadotropin-releasing hormone (GnRH) was carried out on the anadromous salmonoid fish,Plecoglossus altivelis altivelis, commonly known as Ayu. River fish caught were acclimatized in a freshwater aquarium, half of them being subsequently kept as a control group and the remainder being transferred to a sea water aquarium, through 1/3 diluted sea water, as an experimental group. OXT-like immunoreactivity as demonstrated in the neurosecretory pathway, having the same pattern was that shown by aldehyde fuchsin staining. Noticeably, a mass of nucleus preopticus (NPO) and a marginal portion of the pars nervosa in the control group became strongly immunoreactive, whereas a very weak reaction was obtained in the sea water-retained fish, suggesting the release of the labelled substance. In the latter, NPY-like substance was widely distributed in the brain without NPO, with the positive substance being dense in the terminal rami of the pars nervosa bordering the pars distalis. However, no remarkable difference in GnRH-like and NPY-like immunoreactivities in the hypothalamo-hypophysial system was apparent between the two groups. These results suggested that OXT (probably isotocin)-like substance may play a role in osmoregulation.

Distribution of glucocorticoid receptors in the brain of rainbow trout (Oncorhynchus mykiss): possible relationships with the neuroendocrine systems controlling reproduction

The distribution of the glucocorticoid receptor (GR)within the forebrain of rainbow trout has been studied by means of in situ hybridization (ISH) and immunohistochemistry (ICC) using probes and antibodies directed against the AB-domain of the rainbow trout GR (rtGR). Both rtGR mRNA and protein exhibited the same pattern of distribution. A strong labelling was observed in all subdivisions of the preoptic nucleus, in the thalamic region and in the mediobasal hypothalamus (nucleus recessus lateralis, nucleus lateralis tuberis).It was notably found that all magnocellular neurons of the nucleus preopticus pars magnocellularis (NPOmc) were positive with both ISH and ICC. Furthermore, numerous cells of the anterior lobe of the pituitary were positively stained. Double staining studies allowed to identify part of the rtGR expressing cells. At the telencephalic level, it was found that GnRH neurons frequently express rtGR in vitellogenic females. Virtually all tyrosine hydroxylase (TH) cells in the anteroventral part of the preoptic nucleus (NPOav) were rtGR-positive. In this same area, a colocalization between rainbow trout estradiol receptor (rtER) and TH has already been documented, indirectly demonstrating that rtER and rtGR are colocalized. The same holds true for the GTH-II cells of the anterior pituitary which express both types of receptors. These results provide information on the potential sites of cortisol action on the reproductive axis directly at the pituitary level or indirectly on neuroendocrine Systems controllingGTH-II secretion, possibly via GR-ER interactions.

Neurons of the posterior subdivision of the nucleus preopticus periventricularis project to the preglomerular nucleus in the weakly electric fish, Apteronotus leptorhynchus

By using an in vitro tract-tracing technique, the neural connections between two diencephalic cell groups, the posterior subdivision of the nucleus preopticus periventricularis (PPp) and the preglomerular nucleus (PG), was examined in the weakly electric gymnotiform fish Apteronotus leptorhynchus. Neurons of the PPp project to one area within PG, the ventromedial cell group of the medial subdivision of the preglomerular nucleus (PGm-vmc). Axons of these cells reach the ipsilateral PGm-vmc via the basic hypothalamic tract, while collaterals decussate via the postoptic commissure to innervate the contralateral PGm-vmc. We hypothesize that those neurons within PPp that project to the PGm-vmc are homologous to neurons of the medial preoptic area of mammals. As part of an elaborate circuit, PPp and PG may participate, as in mammals, in the control of complex social behavior patterns.

A distinct population of neurons in the central posterior/prepacemaker nucleus project to the nucleus preopticus periventricularis in the weakly electric gymnotiform fish, Apteronotus leptorhynchus

The central posterior/prepacemaker nucleus of weakly electric gymnotiform fish is a cell cluster in the dorsal thalamus involved in neural control of electric behaviors. By employing anterograde and retrograde tract-tracing techniques, we examined the neural connection between this complex and the preoptic area in Apteronotus leptorhynchus. Unilateral application of biocytin restricted to the region defined by the somata of the central posterior/prepacemaker nucleus revealed a network of fibers and terminals bilaterally in the anterior and posterior subdivisions of the nucleus preopticus periventricularis. Application of biocytin to the nucleus preopticus periventricularis demonstrated that these fibers arise from a small population of cell bodies located predominantly in the central and medial portions of the central posterior/prepacemaker nucleus. These somata were distinguished from the remaining cells in this complex not only by their pattern of connectivity, but also by their position within the cluster and by the relatively large size. The projection from the central posterior/prepacemaker nucleus to the nucleus preopticus periventricularis may provide a feedback loop complementing a recently described connection projecting from the preoptic area to the central posterior/prepacemaker nucleus with one synaptic link in the preglomerular nucleus.

Distribution of NADPH-diaphorase activity in the hypothalamo-hypophysial system of the frog, Rana esculenta

Using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase (ND) histochemistry, this study reports a wide distribution of ND activity in the hypothalamus, and for the first time in the median eminence (ME), the neural lobe (NL) and in the pars distalis (PD) of the frog, Rana esculenta. Perikarya are ND-active within the nucleus preopticus (NPO), the nucleus preopticus periventricularis (NPP), located around the preoptic recess (PR), the suprachiasmatic nucleus (SCN) and within five infundibular nuclei. Several ND-positive neurons of the nucleus infundibularis ventralis are cerebrospinal fluid-contacting in nature, while a few occupy a subependymal region. The infundibulum shows a thick sheet-like fiber plexus which receives fibers not only from its ND-active neurons, but also from the anterior and central thalamic nuclei. The ME, NL and most cells of the posterodorsal PD are ND-positive. The pituitary function may be mediated by nitric oxide through modulating the secretion of hormone-releasing factors of the hypothalamus. Possible functional significance of the ND-stained hypothalamic areas is discussed.

Localization of Progesterone Receptor in Brain and Pituitary of the Ring Dove: Influence of Breeding Cycle and Estrogen

An immunocytochemical method was used in male and female ring doves (Streptopelia risoria) to localize progesterone receptor immunoreactivity (PR-ir) in the brain and anterior pituitary gland in nonbreeding, incubating, brooding, and estrogen (E2)-treated nonbreeding birds. Progesterone receptor was found in four regions of the brain in males and females: the preoptic area (POA), nucleus preopticus paraventricularis magnocellularis (PPM), nucleus hypothalami lateralis (PLH), and the tuberal region (TR). Quantitative analysis demonstrated that the density of cell nuclei containing PR-ir in the POA, PPM, and PLH in brooding birds was significantly higher than in E2-treated doves or in birds at other stages of the reproductive cycle. The density of PR-containing cell nuclei in the TR of male ring doves was significantly higher on day 1 of incubation than in nonincubating males. In brooding birds, there was a significant decrease in PR-ir in the TR, particularly in the ventral region where nuclei containing PR-ir disappeared. In the anterior pituitary gland the density of cell nuclei containing PR-ir was higher in females than in males at the onset of incubation. E2treatment resulted in an increase in the density of PR-containing cell nuclei in both males and females. Brooding females had a lower concentration of PR-containing cell nuclei than did females at other stages of the breeding cycle. It is suggested that progesterone receptor in the POA mediates the expression of incubation behavior while progesterone receptor in the TR is involved in the control of neuroendocrine function. The source of estrogen which increases PR appears to be of central nervous origin in the male and may be predominantly peripheral in the female.

An immunocytochemical study of encephalic photoreceptors in three species of lamprey

The extraretinal and extrapineal photoreceptors of three species of adult lamprey, sea lamprey (Petromyzon marinus), river lamprey (Lampetra fluviatilis) and silver lamprey (Ichthyomyzon unicuspis) were studied using antibodies raised against photoreceptor rod and cone opsins, alpha-transducin and arrestin. In all three species cells in the pineal organ (P), parapineal organ (PP), nucleus preopticus (T5), nucleus commissurae postopticae (D8), nucleus ventralis hypothalami (D10) and nucleus dorsalis hypothalami (D11) were labelled by one or more of the anti-opsin antibodies. In addition, anti-arrestin antibodies labelled cells within the D8 and anti-alpha-transducin antibodies labelled cells within the pineal complex and hypothalamus (primarily D8 and/or D10). A more variable and species dependent pattern of opsin, arrestin and alpha-transducin labelling was observed within the nucleus commissurae postinfundibularis (D12) in an area comprising the nucleus dorsalis thalami pars subhabenularis (D4sh) and nucleus dorsalis thalami pars caudalis/nucleus commissurae posterioris (D4c/M1), and in the proximity of the second Müller cells in the ventrocaudal diencephalon (2.MZ/M6). The majority of the neurons labelled within the pineal and parapineal organs and hypothalamus were periventricular with clear cerebrospinal fluid contacts (CSF-contacting neurons). Labelled neurons in the epithalamic (D4sh and D4c/M1) and caudal diencephalon (2.MZ/M6) had no obvious ventricular contacts. We speculate that the "primitive" vertebrate brain of lampreys represents an ancestral condition in which different populations of encephalic photoreceptors are associated with different behavioural and physiological responses. Image-forming vision needs an eye, but irradiance detection does not require a specialised organ. Rather the photoreceptors could be closely associated with their effector systems within the brain.

The Study of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP)-like Immunoreactivity in the Brain of a Teleost, Stargazer, Uranoscopus japonicus

Pituitary adenylate cyclase activating polypeptide (PACAP), a member of the secretin/glucagon/vasoactive intestinal polypeptide family of peptides, consists of a 38-residue (PACAP 38) and a truncated 27-residue (PACAP 27) form that play several roles in mammals. Recently, we isolated a PACAP-like peptide with a sequence highly homologous with that of tetrapod PACAP from the brain of a teleost, the blue spotted-stargazer (Gnathagnus elongatus). In this study, a PACAP-like peptide obtained from the brain of the stargazer, Uranoscopus japonicus, was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting analysis using an anti-PACAP 27 serum. The stargazer PACAP-like peptide and synthetic human PACAPs were reacted with this antiserum. The distribution of PACAP-like immunoreactivity in the stargazer brain was also studied immunohistochemically using the peroxidase-antiperoxidase method. PACAP-like immunoreactive (LI) cells were found in the nucleus preopticus, pars parvicellularis of the hypothalamus. PACAP-LI nerve fibers and terminals were observed in the anterior neurohypophysis and in the rostral pars distalis, and many PACAP-LI nerve fibers were seen in the medulla oblongata. These results suggest that PACAP-like peptide may be involved in pituitary regulation and/or other neural functions in the stargazer brain.

Distribution and expression of glucocorticoid receptor mRNA in the forebrain of the rainbow trout.

The expression and distribution of glucocorticoid receptor mRNA was studied in the forebrain of mature female and immature undifferentiated rainbow trout (Oncorhynchus mykiss) by means of Northern blotting and in situ hybridization. A single mRNA species of 7.5 kb was detected in mRNA polyA+ prepared from the anterior brain. In situ hybridization was carried out using a 35S-labelled riboprobe corresponding to the A/B-domain (between nucleotides 1224 and 1763) of the recently cloned rainbow trout glucocorticoid receptor cDNA. Comparison of adjacent sections hybridized with the sense and antisense probes allowed detection of a specific signal with a similar distribution pattern in all animals studied. In the telencephalon, a specific hybridization was detected in scattered cells of the dorsal telencephalic hemisphere, but the stronger signal was consistently observed in the dorsal nucleus, and to a lesser degree in the ventral nucleus of the ventral telencephalon. Heavy hybridization staining was consistently observed in all subdivisions of the preoptic nucleus and the nucleus lateralis tuberis, which are the main hypophysiotrophic regions in fish. A weaker signal was detected in the nucleus anterioris periventricularis, nucleus suprachiasmaticus and thalamic region. The presence of a strong signal in virtually all magnocellular neurons of the nucleus preopticus, known for producing vasotocin, isotocin and corticotropin-releasing factor favors a direct function of cortisol in regulating its own secretion.

Endocrinology of sound production in fishes

Fish sounds are often associated with agonistic and courtship behavior, and in many cases sounds are produced by males with sexually dimorphic sound‐producing mechanisms. This review focuses on the sounds and sexually dimorphic sonic neuromuscular system of the oyster toadfish Opsanus tau, compares it with findings on a related toadfish, the midshipman Porichthys notatus and finally reviews data on steroid levels and their effect on seasonal changes in mass of the sonic muscles and sound characteristics in the weakfish Cynoscion regalis. Highlights of the work on the oyster toadfish include the following. (1) Quantitative seasonal changes in the male boatwhistle mating call in a manner suggesting that steroids modulate the output of central nervous system pattern generators for fundamental frequency and duration. (2) Larger sonic muscles in males comprised of a greater number of smaller and therefore more energy efficient fibers than in females. Male muscles have greater activities of anaerobic and aerobic enzymes and enzyme activities and muscle size are increased by andro‐gens. (3) Neurons in the spinal sonic motor nucleus (SMN) are small in females and either small (S) or large (L) in males. The same three morphs are present in Porichthys, but there are major differences in somatic growth patterns, dendrite development and sonic abilities among morphs in the two species. Development of sexual dimorphism in the SMN suggests major differences between amniotic vertebrates (birds and mammals) and fishes, particularly with regard to perinatal critical periods and cell death. (4) As in other vertebrates, toadfish sonic centers in the central nervous system (including the supracommissural nucleus of the ventral telencephalon, nucleus preopticus parvocellularis anterior, the torus semicircularis and the inferior reticular nucleus) possess gonadal steroid‐concentrating neurons. Although endocrine investigation of sound production has been attempted in only three species, its relevance to important life history events of commercially‐important species and the availability of large numbers of basic biological questions suggest the field is ripe for further investigation.

Autoradiographic localization of receptors for neuropeptide Y (NPY) in the brain of broiler and leghorn chickens ( Gallus domesticus)

Broiler and leghorn chickens show an extreme difference in ingestive and reproductive behavior. As neuropeptide Y (NPY) influences both behaviors the goal of this study was to elucidate the distribution, expression and affinity of NPY binding sites in broiler and leghorn chicken brain. By means of in vitro autoradiography, sections of chicken brains were incubated with 3H-NPY as tracer and NPY as displacer. Scatchard analysis revealed a curvilinear plot suggesting two subtypes of the NPY binding site in the chicken brain, a high affinity one ( K D = 2–4 nM) and one with a lower affinity ( K D = 18–24 nM). Binding sites for NPY are localized with high density in the different subdivisions of the neostriatum and the hyperstriatum, the cerebellum, the nucleus septalis lateralis and medialis, the nucleus ruber and the nucleus tractus solitarii. A lower density of NPY binding sites was found in the different subdivisions of the striatum, the nucleus mesencephalicus lateralis pars dorsalis, the paleostriatum, the archistriatum intermedium pars ventralis, the nucleus geniculatus lateralis, the nucleus taeniae, the locus ceruleus, the nucleus rotondus, the nucleus habenularis medialis, the nucleus dorsomedialis anterior (rostralis) thalami, the pituitary and the area of the hypothalamus with its nuclei such as the nucleus paraventricularis magnocellularis and the nucleus preopticus medialis. Comparison of the localization of NPY binding sites in the brains of broilers and leghorns showed no differences but the density of both receptor types is two to three times higher in broilers than in leghorns.

Immunoreactive gonadotropin-releasing hormone (GnRH) in the brain and pituitary of adult and juvenile swordtails (Xiphophorus helleri, Teleostei, Poeciliidae).

Different molecular variants of gonadotropin-releasing hormone (GnRH) were localized in the brain and pituitary of Xiphophorus helleri, from neonates up to mature animals of both sexes. Nine GnRH antisera to salmon (s), mammalian (m), chicken I (c-I), and chicken II (c-II) GnRH were utilized. In the first week after birth GnRH immunoreactivity (IR) emerges with pale staining of the nucleus olfactoretinalis (NOR) in the ventral forebrain. The intensity of IR in the NOR increases during the next weeks and an IR tract of nerve fibers appears, protruding from the NOR in dorsocaudal direction. Adult animals exhibit additional GnRH-positive structures. Some perikarya of the nucleus preopticus periventricularis (NPP) are IR and positive fibers extend from the NPP toward the pituitary. In the pituitary IR fibers are also detectable. A distinctive structure in adult animals is an IR cord of neurons (CN) at the bottom of the forebrain which extends from the NPP to the olfactory nerve. A comparison of antisera against different GnRH species indicates that sGnRH is present in the NOR, whereas a different form of GnRH is present in the NPP, CN, and pituitary. The early onset of GnRH IR in the NOR and the widespread distribution of positive fibers from that nucleus into other brain regions suggest neuromodulatory functions of sGnRH from the NOR. The NPP possibly plays a major role in direct stimulation of pituitary gonadotropes via a different type of GnRH. © 1996 Wiley-Liss, Inc.