Notable Presence Research Articles

A novel vaccine strategy against Brucellosis using Brucella abortus multi-epitope OMPs vaccine based on Lactococcus lactis live bacterial vectors

Brucella infections typically occur in mucosal membranes, emphasizing the need for mucosal vaccinations. This study evaluated the effectiveness of orally administering Lactococcus lactis (L. lactis) for producing the Brucella abortus multi-epitope OMPs peptide. A multi-epitope plasmid was generated through a reverse vaccinology method, and mice were administered the genetically modified L. lactis orally as a vaccine. The plasmid underwent digestion, synthesizing a 39 kDa-sized protein known as OMPs by the target group. The sera of mice that were administered the pNZ8124-OMPs-L. lactis vaccine exhibited a notable presence of IgG1 antibodies specific to outer membrane proteins (OMPs), heightened levels of interferon (IFN-λ) and tumor necrosis factor alpha (TNF-α), and enhanced transcription rates of interleukin 4 (IL-4) and interleukin 10 (IL-10). The spleen sections from the pNZ8124-OMPs-L. lactis and IRIBA group had less morphological damage associated with inflammation, infiltration of lymphocytes, and lesions to the spleen. The findings present a novel approach to utilizing the food-grade, non-pathogenic L. lactis as a protein cell factory to synthesize innovative immunological candidate OMPs. This approach offers a distinctive way to evaluate experimental medicinal items' practicality, safety, affordability, and long-term sustainability.

Impact of subthalamic nucleus deep brain stimulation at different frequencies on neurogenesis in a rat model of Parkinson's disease

Neurogenesis, play a vital role in neuronal plasticity of adult mammalian brains, and its dysregulation is present in the pathophysiology of Parkinson's disease (PD). While subthalamic nucleus deep brain stimulation (STN-DBS) at various frequencies has been proven effective in alleviating PD symptoms, its influence on neurogenesis remains unclear. This study aimed to investigate the effects of 1-week electrical stimulation at frequencies of 60Hz, 130Hz, and 180Hz on neurogenesis in the subventricular zone (SVZ) of PD rats. A hemiparkinsonian rat model was established using 6-hydroxydopamine and categorized into six groups: control, PD, sham stimulation, 60Hz stimulation, 130Hz stimulation, and 180Hz stimulation. Motor function was assessed using the open field test and rotarod test after one week of STN-DBS at different frequencies. Tyrosine hydroxylase (TH) expression in brain tissue was analyzed via Western blot and immunohistochemistry. Immunofluorescence analysis was conducted to evaluate the expression of BrdU/Sox2, BrdU/GFAP, Ki67/GFAP, and BrdU/DCX in bilateral SVZ and the rostral migratory stream (RMS). Our findings revealed that high-frequency STN-DBS improved motor function. Specifically, stimulation at 130Hz increased dopaminergic neuron survival in the PD rat model, while significantly enhancing the proliferation of neural stem cells (NSCs) and neuroblasts in bilateral SVZ. Moreover, this stimulation effectively facilitated the generation of new NSCs in the ipsilateral RMS and triggered the emergence of fresh neuroblasts in bilateral RMS, with notable presence within the lesioned striatum. Conversely, electrical stimulation at 60Hz and 180Hz did not exhibit comparable effects. The observed promotion of neurogenesis in PD rats following STN-DBS provides valuable insights into the mechanistic basis of this therapeutic approach for PD.

Histological Assessment of the Effectiveness of Microneedling Device-Assisted Filler Delivery.

Microneedling is used to enhance transcutaneous drug delivery. However, the extent to which microneedling devices impact filler delivery and whether this varies by filler type, microneedling device type, and treatment sequence is not known. To histologically assess and quantify the delivery of commonly used fillers through microneedling, using both a microneedling pen and a microneedling roller. In addition, the authors investigated whether there is a variation in filler delivery based on the sequence of microneedling in relation to topical filler application. Ex vivo human abdominal skin samples were subjected to microneedling pen or microneedling roller treatment. Black tissue marking ink, hyaluronic acid, poly- l -lactic acid, or undiluted calcium hydroxyapatite was topically applied before or immediately after microneedling treatment. Histological evaluation revealed a notable presence of black ink within channels formed by both microneedling treatments (15.5%-98.1%), whereas there was limited presence of the various filler types tested (0%-6.6%) in all settings. Topical application before microneedling treatment led to relatively higher filler/ink deposition within the channels formed by the microneedling treatments compared with topical application after microneedling. Transcutaneous delivery of fillers was not significantly helped by microneedling treatment, whereas the microneedling devices demonstrated effective delivery of an aqueous solution.

Laser-inherent porosity defects in additively manufactured Ti–6Al–4V implant: Formation, distribution, and effect on fatigue performance

Porosity defects are inherently present in Ti–6Al–4V (Ti6-4) parts produced using additive manufacturing (AM) methods like laser powder-bed fusion (LPBF). This work aims to investigate different laser-inherent porosity defects at various LPBF parameter settings and assess their impact on the fatigue behaviour of Ti6-4 implants processed by LPBF. The presence of LPBF-inherent porosity defects with different shapes and sizes was established using microstructural examination and X-ray micro-CT analysis. These mostly comprise lack-of-fusion porosity (LoFP), gas-entrapped porosity (GeP), and pores-induced microcracks. Volumetric porosity defects were seen to range from 1.9 × 104 to 9.52 × 105 μm3. The L-1 specimen exhibited the lowest defect, while the L-6 specimen displayed the largest number of defects. While LoFP defects predominate in L-6, there was a notable presence of GeP defects in the specimens processed using the factory default condition (L-D). Upon examination of the majority of specimens, GeP and LoFP coalesced to form clusters, leading to the formation of pores-induced microcracks. This ultimately leads to a decrease in fatigue performance. By maintaining the power at the default setting and increasing the scan speed by 8% of the default value, a specimen (L-1) with minimal porosity defects and superior fatigue performance is achieved. L-6 exhibits defects with significant dimensions and irregular form. Consequently, it displays inferior fatigue characteristics.

Effect of annealing temperature on the evolution of microstructure, texture, and mechanical properties of hot-rolled 12Cr-ODS steel

To achieve high thermal efficiency, today's nuclear reactor structures are exposed to higher temperatures and pressures, which requires the use of high-strength steels with specific properties, such as ODS steels. There is a need to clarify the evolution of the microstructure and properties of steels at elevated temperatures. This study systematically investigates the evolution of microstructure, texture, and mechanical property variations of 12Cr-ODS steels after hot-rolling and subsequent annealing at 1000 °C and 1200 °C. The investigation utilizes optical microscopy, electron backscatter diffraction technique, and mechanical property measurements. The microstructure of hot-rolled samples shows a layered alternating distribution, which is distinguished by a notable presence of the α-fiber texture. Following annealing at 1000 °C, the martensite grains are smaller with a reduced hardness, while maintaining a strong α-fiber texture. After annealing at 1200 °C, there is a rapid increase in the growth of martensite grains, a significant rise in hardness, a reduction in the α-fiber texture characteristics, and an improvement in the γ-fiber texture characteristics. Moreover, the maximum intensity of the α-fiber texture diminishes as the annealing temperature increases. The mechanical properties of the samples deteriorated after annealing at 1200 °C, which can be attributed to the coarse martensite grains and the texture components containing the {001} cleavage plane dominating the occurrence of brittle cleavage fracture. The 0.1Y sample after annealing at 1000 °C exhibits an excellent combination of strength (1458 MPa) and ductility (20.3%), which is owing to the unique heterogeneous grain structure and the evolution of favorable texture.

Optimization of Gold Pawn Product Advantages to Increase Customer Base

The objective of this study is to examine the main characteristics that impact customer satisfaction with pawn products at BPRS Bhakti Sumekar, with a specific focus on its Waru Branch. Data for this study were gathered using a qualitative descriptive approach, through interviews conducted with the management, employees, and customers of BPRS Bhakti Sumekar's Waru Branch. In addition, supplementary data were acquired from papers, journals, and internal records of the bank. The research aims to maximize the benefits of gold pawn products in order to broaden the consumer base. BPRS Bhakti Sumekar, previously named BPR Bhakti Sumekar, is a Regional-Owned Enterprise (BUMD) located in Sumenep Regency. It was founded on September 16, 2002, first as a limited liability company (PT) before converting to a BUMD status. BPRS Bhakti Sumekar has a notable presence in the local financial sector with two Cash Offices and 28 Branch Offices, one of which is the Waru Branch.

Development of gonad and brood pouch and expression profiles of cgα, fshβ and lhβ transcripts and plasma 17β-E2 and 11-KT during the first reproductive cycle of farmed big-belly seahorse (Hippocampus abdominalis)

Limited data are available regarding the gonadal development of farmed seahorse along with the physiological role of gonadotropins and sex steroids during gonadal maturation. In the present study, we conducted an examination of the developmental stages of gonad and brood pouch during the initial reproductive cycle of the farmed big-belly seahorse (Hippocampus abdominalis), and our results showed that males sexually matured half of a month later than females, and the brood pouch emerged at 3.5-month old and then reached the late-stage at 5.5-month old. Meanwhile, the open reading frame (ORF) of gonadotropin subunits (cgα, fshβ and lhβ) of the big-belly seahorse were cloned and characterized, and tissue distribution profiles of their transcripts were analyzed, revealing their detectability across various tissues, with a notable presence observed in the brain tissues. Furthermore, expression profiles of cgα, fshβ and lhβ transcripts and the plasma levels of 17β-estradiol (E2) and 11-ketotestosterone (KT) in farmed big-belly seahorse during the first reproductive cycle were also investigated. The findings indicated that cgα, fshβ and lhβ transcripts exhibited low expressions at the initial development of ovary or testis, raised continuously and reached their peak levels at the period of spawning or spermation, and they kept gradually increasing accompany with brood pouch growth. It is noteworthy that variation of mRNA levels of fshβ were slightly higher than those of lhβ. Besides, the results showed that plasma 11-KT levels presented an increasing trend during testicular development, peaking in fish with III and IV-stage testis and early-stage brood pouch, and plasma 17β-E2 levels were higher in vitellogenic stage of fish with II-stage ovary and early III-stage ovary. Taken together, these data reveal that gonadotropins and sex steroids likely play a role in the regulation of gonad and brood pouch growth in seahorse. These observations not only contribute to clarifying function of reproductive hormones on growth of gonad and brood pouch of seahorse, but also providing an important theoretical foundation for the artificial breeding of seahorses.

Cell redistribution of G quadruplex-structured DNA is associated with morphological changes of nuclei and nucleoli in neurons during tau pathology progression.

While the double helical structure has long been its iconic representation, DNA is structurally dynamic and can adopt alternative secondary configurations. Specifically, guanine-rich DNA sequences can fold in guanine quadruplexes (G4) structures. These G4 play pivotal roles as regulators of gene expression and genomic stability, and influence protein homeostasis. Despite their significance, the association of G4 with neurodegenerative diseases such as Alzheimer's disease (AD) has been underappreciated. Recent findings have identified DNA sequences predicted to form G4 in sarkosyl-insoluble aggregates from AD brains, questioning the involvement of G4-structured DNA (G4 DNA) in the pathology. Using immunofluorescence coupled to confocal microscopy analysis we investigated the impact of tau pathology, a hallmark of tauopathies including AD, on the distribution of G4 DNA in murine neurons and its relevance to AD brains. In healthy neurons, G4 DNA is detected in nuclei with a notable presence in nucleoli. However, in a transgenic mouse model of tau pathology (THY-Tau22), early stages of the disease exhibit an impairment in the nuclear distribution of G4 DNA. In addition, G4 DNA accumulates in the cytoplasm of neurons exhibiting oligomerized tau and oxidative DNA damage. This altered distribution persists in the later stage of the pathology when larger tau aggregates are present. Still cytoplasmic deposition of G4 DNA does not appear to be a critical factor in the tau aggregation process. Similar patterns are observed in neurons from the AD cortex. Furthermore, the disturbance in G4 DNA distribution is associated with various changes in the size of neuronal nuclei and nucleoli, indicative of responses to stress and the activation of pro-survival mechanisms. Our results shed light on a significant impact of tau pathology on the dynamics of G4 DNA and on nuclear and nucleolar mechanobiology in neurons. These findings reveal new dimensions in the etiopathogenesis of tauopathies.

Distinct Adverse Reactions to mRNA, Inactivated Virus, and Adenovirus Vector COVID-19 Vaccines: Insights from a Cohort Study on Atopic and Non-Atopic Subjects in Brazil.

The emergence of COVID-19 caused by SARS-CoV-2 prompted an unprecedented global response to develop vaccines at an accelerated pace. Messenger RNA (mRNA) and adenovirus vector vaccines emerged as the frontrunners in global immunization efforts, significantly reducing hospitalization, severity, and mortality, supplemented by inactivated virus-based vaccines in developing countries. However, concerns regarding adverse effects, including allergic reactions, have been raised. This study aimed to investigate the adverse effects following COVID-19 vaccination, particularly in atopic and non-atopic individuals. A cohort of 305 volunteers receiving BNT162, ChAdOx1, or CoronaVac vaccines were assessed based on a Skin Prick Test (SPT), specific IgE levels, and clinical history of asthma and rhinitis. Adverse effects were self-reported and scored across the different vaccination shots. The results indicated a notable presence of mild adverse effects following the first and third doses, regardless of vaccine type. ChAdOx1 recipients experienced more adverse effects compared to those receiving BNT162 and CoronaVac, including headaches, muscle pain, fever, chills, nausea, and flu-like symptoms. Atopic individuals receiving ChAdOx1 reported more adverse effects, such as muscle pain, fever, and chills, compared to non-atopic individuals. Conversely, headaches were more frequently reported in non-atopic individuals receiving BNT162 compared to atopic individuals. No anaphylaxis or allergic reactions were reported, indicating valuable evidence supporting the safety of COVID-19 vaccination in individuals with respiratory allergies. This study highlights the importance of understanding vaccine-related adverse effects, particularly in vulnerable populations, to inform vaccination strategies and address safety concerns in global immunization campaigns.

Morphometric Analysis Using Geographical Information System and the Relationship with Precipitation Quantiles of Major Dam Basins in South Korea

Geographic information system (GIS) and remote sensing (RS) technologies are potent tools for evaluating various aspects of basin hydrology. This study conducted a morphological analysis using GIS tools on seven major dam basins in Korea. Additionally, long-term RS-based precipitation data were obtained, the probability of precipitation was estimated, and their relationship was examined. The findings are summarized as follows. It was observed that most major dam basins in Korea, which were the focus of this study, had a broad radial shape, and due to the mountainous topography, there was a notable presence of numerous river branches. Through the estimation of probability precipitation and its comparison with morphological indices, it was noted that wider basins tend to have higher rainfall amounts and a relatively uniform spatial distribution. Furthermore, it was found that the more uniform the spatial distribution, the simpler the river network. This trend becomes more pronounced in relation to basin size for longer durations, and in spatial dispersion for shorter durations. However, it is crucial to acknowledge that the complexity of these relationships is also affected by other factors such as climate, altitude, and local geographical conditions.

Soil structure and solute transport pathways in biogas digestate-amended soils

Here, we investigated the effects of biogas digestate application on flow pathways and soil properties in a sandy soil aiming at unraveling phosphorus accumulation and depletion processes in fertilized soils. Dye tracer experiments were conducted to visualize and distinguish between various flow pathways, while X-ray tomography was utilized to characterize the soil structure. The dye tracer experiments revealed differences between the control and the digestate-treated group of soil profiles in the top-soils although no statistical verification was possible amid a single plot per treatment. The stained soil profiles in both treatments indicate extensive areas of homogeneous and heterogeneous matrix flow. However, the digestate-treated group showed a decrease in homogeneous matrix flow and a notable presence of preferential flow with low interaction. The application of biogas digestate impacted various soil properties, including organic carbon content, water drop penetration time, and pH levels. Accordingly, the topsoil experienced an increase in soil organic carbon, water repellency, and a decrease in pH levels. The fraction of macropores also increased in the topsoil of the digestate-treated group, accompanied by an increase in tortuosity.Double lactate-extractable phosphorus (DL-P) distribution varied across the different flow pathways, with higher concentrations detected in matrix flow and a depletion observed in preferential flow regions in both treatments. These findings confirm the close relationship between the type of flow pathway and phosphorus distribution in soils. Additionally, a significant correlation was observed between pH levels and DL-P in the digestate-treated group, indicating potential implications for phosphorus availability. Overall, the results of this study suggest that the long-term application of biogas digestate is likely to improve soil properties as related to soil organic matter content, particularly in the topsoil of sandy soils. The study highlights the significance of the prevailing flow and transport pattern on nutrient distribution, with potential implications for nutrient management in agricultural systems. While the study design presented prevents the unequivocal assignment of differences to treatment effects, our data likely illustrate the impacts of biogas digestate amendment on the investigated sandy soil.Further research should focus on assessing the long-term effects of digestate application in loamy and clayey soils.

The role of olfactory dysfunction in mild cognitive impairment and Alzheimer's disease: A meta-analysis

PurposeThis comprehensive meta-analysis investigates the association between olfactory deficits in mild cognitive impairment (MCI) and Alzheimer's disease (AD). MethodsA thorough search across databases identified articles analyzing olfactory status in MCI or AD patients. Methodological quality assessment followed PRISMA guidelines. Hedges' g effect size statistic computed standard mean differences and 95% confidence intervals. Moderator analysis was conducted. ResultsAmong the included studies (65 for MCI and 61 for AD), odor identification exhibited larger effect sizes compared to odor threshold and discrimination, in both MCI and AD samples. Moderate effect size is found in OI scores in MCI (k = 65, SE = 0.078, CI 95% = [-1.151, -0.844]). Furthermore, compared to MCI, AD had moderate to large heterogeneous effects in olfactory identification (k = 61, g = -2.062, SE = 0.125, CI 95% = [-2.308, -1.816]). Global cognitive status is positively related to olfactory identification impairment in both MCI (k = 57, Z = 2.74, p = 0.006) and AD (k = 53, Z = 5.03, p < 0.0001) samples. ConclusionOlfactory impairments exhibit a notable and substantial presence in MCI. Among these impairments, odor identification experiences the greatest decline in MCI, mirroring the primary sensory deficit observed in AD. Consequently, the incorporation of a straightforward odor identification test is advisable in the evaluation of individuals vulnerable to the onset of AD, offering a practical screening tool for early detection.

Strategies for Imputing Missing Values and Removing Outliers in the Dataset for Machine Learning-Based Construction Cost Prediction

Accurately predicting construction costs during the initial planning stages is crucial for the successful completion of construction projects. Recent advancements have introduced various machine learning-based methods to enhance cost estimation precision. However, the accumulation of authentic construction cost data is not straightforward, and existing datasets frequently exhibit a notable presence of missing values, posing challenges to precise cost predictions. This study aims to analyze diverse substitution methods for addressing missing values in construction cost data. Additionally, it seeks to evaluate the performance of machine learning models in cost prediction through the removal of conditional outliers. The primary goal is to identify and propose optimal strategies for handling missing value in construction cost records, ultimately improving the reliability of cost predictions. According to the analysis results, among single imputation methods, median imputation emerges as the most suitable, while among multiple imputation methods, lasso regression imputation produces the most superior outcomes. This research contributes to enhancing the trustworthiness of construction cost predictions by presenting a pragmatic approach to managing missing data in construction cost performance records, thereby facilitating more precise project planning and execution.

Abstract 3934: Smoking's influence on divergent paths to lung cancer

Abstract Lung cancer is the leading cause of cancer mortality in the world. Many studies have highlighted how mutagens from cigarette smoke promote specific mutations such as G12 mutations in KRAS, contributing to clonal evolution that leads to lung cancer. Despite the indisputable correlation between smoking and lung cancer, people who have never smoked account for the 7th leading cause of cancer mortality. Given the differences in predominant exposures, we hypothesize that the clonal evolution between people with and without smoking histories are markedly different. Therefore, we analyzed lung samples from people with different cigarette smoking exposure to elucidate smoking’s effect on the somatic mutational landscape in the progression to lung cancer. To observe somatic mutations subjected to different exposures of smoking, we used a rare-mutation detection technique called DuplexSeq to analyze somatic variants from 200+ lung samples. We acquired DNA from cells from bronchoscopy brushings and lung punches of histologically normal tissue from people with and without lung cancer and who currently, formerly, or never smoked cigarettes. Through collaborations, we characterized mutations using bioinformatic pipelines, various databases, and statistical methods. In samples from people who smoke, we observe an enhancement of mutations with higher variant allele frequencies and pervasive positive selection acting on mutations in many cancer-associated genes. From our bronchoscopy brushings, NOTCH1 mutations show the most definitive increase of protein-altering variants, while NF1 and KRAS show a predominance of driver mutation in lung punches. Unexpectedly, people without lung cancer who never smoked had a notable presence of pathogenic mutations in PTEN and PIK3CA. Furthermore, multiple punches from the same individual highlight how expansions of mutations have stronger similarity within an individual than across individuals regardless of smoking status. This association points to the importance of the inherent lung microenvironment in influencing the mutational landscapes in lung cancer progression. Altogether, these results show how smoking increases the oncogenicity of observable mutations in the lung but does not preclude the presence of oncogenic clones, emphasizing the value of precision diagnoses and prevention. Ongoing research will be centered around functional assays to determine the impact of observed mutations on tissue function and longitudinal studies using mutational landscapes as a proxy for lung cancer risk. Citation Format: Edward James Evans, Fabio Marongiu, Emilia Lim, Oriol Pich, Yonghua Zhuang, Dexiang Gao, William Hill, Cian Murphy, Ryan Turner, Moumita Ghosh, York Miller, Mariam Jamal-Hanjani, Charles Swanton, James DeGregori. Smoking's influence on divergent paths to lung cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3934.

Bacterial Flora and Treatment Strategies in Women With Escherichia coli Urinary Tract Infections.

Introduction This study explores the intricate relationship between bacterial flora and the occurrence of Escherichia coli (E. coli) infections in gynecological patients. It aims to provide insights into the various treatment strategies used to effectively manage bacterial pathogens, especially E. coli infections. By conducting a comprehensive analysis of the bacterial flora in gynecological patients, the study highlights the notable presence of E. coli, promptingfurther investigation into the factors that contribute to its colonization. The objective of the study is to comprehensively investigate and detect urinary tract infections (UTIs) specifically caused by E. coli among gynecological patients. The study aims to delve into bacterial flora prevalence, antibiotic resistance patterns, and potential virulence factors. Through this analysis, the study intends to identify effective strategies for rapid detection and diagnosis of UTIs caused by E. coliby utilizing advanced microbiological and molecular techniques. Furthermore, the study aims to formulate and propose a strategic treatment approach with a particular emphasis on selecting appropriate antibiotics to reduce the risk of severe infections and associated complications. Materials and methods The methodology employed in this study included the isolation and characterization of bacterial strains from clinical samples obtained from gynecological patients. A total of 52 urine specimens were collected from patients with complaints of infection in the urinary tract and infertility. These samples underwent both preliminary and confirmatory microbiological analysis, such as gram staining, biochemical confirmation test, and antibiotic susceptibility, and further proceeded with the multiplex polymerase chain reaction (PCR) technique. The results of PCR and antibiotic susceptibility revealed the specific gene involvement and resistant characteristics of E. coli. Results The findings revealed a total of 32 specimens positive for E. coli, of which 10 patients had infertility complaints and 22 patients had UTIs. The preliminary test, gram staining, showed the gram-negative bacilli E. coli, and the nutrient agar plate revealed smooth circular translucent colonies; MacConkey agar showed pink-colored lactose-fermented colonies; and the blood and chocolate agar plates showed grayish white moist gamma-hemolytic colonies. The biochemical confirmation of E. coli resulted in positive for indole and methyl red tests and negative for Voges-Proskauer and citrate utilization tests. The multiplex PCR analysis confirmed the E. coli strains with the presence of two target genes,stx2d and stx2e. Conclusion To summarize, this study offers valuable insights into the bacterial flora of gynecological patients impacted by E. coli infections, which provides a foundation for the development of precise and efficient treatment strategies. The results emphasize the importance of personalized treatment approaches that consider both the microbiological characteristics of the infection and the evolving landscape of antibiotic resistance. The implication of this research extends to enhancing clinical outcomes and alleviating the burden of E. coli infections in gynecological settings.

ROLE OF LABORATORY DIAGNOSTICS IN THE DENTAL PRACTICE

Laboratory methods play a crucial role in dental practice, as the state of a patient's oral cavity frequently mirrors disruptions in metabolic processes within the body, and can also provide insights into potential pathology affecting individual organs and systems.&#x0D; The aim of this study is to assess the efficacy of employing laboratory methods in patients with periodontal tissue diseases.&#x0D; We conducted examinations on 35 patients diagnosed with chronic generalized periodontitis of degrees II and III, along with 15 patients diagnosed with chronic catarrhal gingivitis. Additionally, a control group comprising 15 patients diagnosed solely through clinical examination methods with generalized periodontitis was included in the study.&#x0D; All patients underwent a comprehensive evaluation, including general and biochemical blood tests. The investigation of periodontal pocket contents involved cytological and microbiological analyses, employing the method of successive serial rinses.&#x0D; The patients diagnosed with generalized periodontitis have demonstrated a slight elevation in leukocyte count to 10-12*109/l upon the assessment of the general clinical blood test, indicating an exacerbation of the pathological process in periodontal tissues.&#x0D; Furthermore, patients with generalized periodontitis have exhibited leukocyte migration in oral fluid exceeding 120, signifying disease exacerbation. Cytological examination of periodontal pocket contents revealed a notable presence of epithelial cells.&#x0D; In the microbiocenoses of both gingival and periodontal pockets, representatives of aerobic and facultative anaerobic microorganisms were predominant. Among patients diagnosed with catarrhal gingivitis, these microorganisms constituted 74.25% of the microbiota, while in those with generalized periodontitis, they accounted for 62.70%. During periods of exacerbated periodontitis, there was an increase in the proportion of anaerobes within the microflora of periodontal pockets, rising from 23.15% to 29.40%.&#x0D; Research results have shown that patients with inflammatory and inflammatory-dystrophic periodontal diseases experience dysbiosis of gingival and periodontal pockets, characterized by a decrease in the antagonism of symbiont bacteria to pathogenic and potentially pathogenic microorganisms. The correct interpretation of laboratory studies carried out over time has not only diagnostic and prognostic significance, but is decisive in the choice of adequate treatment.

Parenting Styles and Mental Health among Adolescents of Nawabshah (SBA), SINDH

Background: Parenting styles are crucial in shaping adolescents' mental health. With varying implications for child development, the authoritative, authoritarian, and permissive parenting styles identified by Baumrind have been subjects of extensive research. The interplay between these parenting approaches and adolescent mental health outcomes remains a pivotal area of inquiry, especially in diverse cultural contexts such as Nawabshah (SBA), Sindh. Objective: This study aimed to identify the predominant parenting styles in SBA and to assess their relationship with the mental health of school-going adolescents. Specifically, it sought to explore the association between authoritative, authoritarian, and permissive parenting styles and the prevalence of depressive symptoms among adolescents. Methods: A descriptive cross-sectional study was conducted with 354 adolescents from both government and private secondary and higher secondary schools in Nawabshah. Participants were selected using non-probability convenience sampling. Data were collected via a survey incorporating the Patient Health Questionnaire (PHQ-9) for assessing mental health and the Parent Authority Questionnaire (PAQ) for evaluating parenting styles. Statistical analysis was performed using SPSS version 25, employing the Pearson correlation coefficient to analyze the relationship between parenting styles and adolescent mental health. Results: The study found a predominance of the authoritative parenting style among participants' parents (56.2%). A significant yet low negative correlation was identified between authoritative parenting and moderate depression in adolescents (r = -.124, p &lt; 0.05). Additionally, combinations of parenting styles were observed, with a notable presence of mixed authoritative-authoritarian practices. Adolescents experiencing authoritative parenting reported higher instances of moderate depression compared to those with other parenting styles. Conclusion: The findings challenge the universally positive portrayal of authoritative parenting, highlighting its complex relationship with adolescent mental health in the SBA context. The study underscores the need for a nuanced understanding of parenting styles and their impact, advocating for culturally sensitive approaches in parenting research.

Unveiling spatial disparities in basic medical and health services: insights from China’s provincial analysis

Based on the panel data of 31 provinces (municipalities and autonomous regions) in China from 2012 to 2019, this paper constructs the evaluation index system of basic medical and health services in China from seven dimensions: medical and health facilities, health expenditure, medical services, traditional Chinese medicine hospital services, maternal and child health care, people’s health and medical security, disease control and public health. The entropy method was used to measure the level of basic medical and health services in China, and its spatial differences and convergence characteristics were further investigated. In this study, we employ the entropy weight method, σ convergence, and β convergence as our primary methodologies. The entropy weight method is used to evaluate the variability of each indicator, determine the weights of indicators, and quantify the information content of the data. σ convergence illustrates the process by which the variance of a sample decreases over time. β convergence refers to the gradual approach of variables within an economic system towards their long-term equilibrium level over time. The results show that: (1) The scores of basic medical and health services in China’s four major regions (including Northeast, East, Central and West) remain in a relatively stable state, with small fluctuations and great room for improvement; (2) There are significant regional differences in the level of basic medical and health services in China, and the intra-regional differences are much greater than the inter-regional differences; (3) There is no significant σ convergence observed in China and its four major regions; however, there is a notable presence of β convergence.

Common proteins analysis of different mammals' mature milk by 4D-Label-Free

The milk proteins from samples of 13 different animals were identified utilizing 4D-Label-Free proteomics technology, leading to the identification of a substantial number of proteins. Among the various samples, Chinese people (CHP) milk proteins exhibited the highest count, with 1149 distinct proteins. Simultaneously, we identified common proteins present in these animal milk. It's notable presence in goat milk contributes to enhancing infant infection resistance, showcasing the beneficial role of lactoperoxidase. Galectin-3 binding protein (Gal-3BP) and tetraspanin in human milk are significantly higher than those in other animals, which determine the prominent antiviral effect of human milk and the important processes related to cell transduction. Furthermore, human milk, camel milk, goat milk and sheep milk proved to be rich sources of milk fat globule membrane (MFGM) proteins. The insights obtained from this study can serve as a foundational framework for exploring the role of different animal milk proteins in disease treatment and the composition of infant formula.

Imaging Characteristics and Hormonal Receptor Correlations in Varieties of Breast Cancer

Background: While there is acknowledgment of the vital role of factors such as the status of histological grades and hormone receptor (HR) in shaping targeted treatment strategies for breast cancer (BC), comprehensive research that unifies the study of imaging features with the evaluation of HR characteristics and histopathological data is notably absent in Albania, creating a critical research gap that this study endeavors to fill. This study aimed to investigate the imaging characteristics observed in ultrasound and the possible correlations between expression levels of HRs in ductal and lobular types of BC to elucidate potential prognostic and therapeutic implications. Methods and Results: This descriptive study, conceived as a series of cases, leveraged a prospective approach to scrutinize the dynamics of the study population over four years (2019-2023) in the Mother Teresa University Hospital Center and a private oncology clinic in Tirana. The convenience sampling strategy enlisted 238 female patients (mean age 60.5±12.5 years) diagnosed with BC who had been tested for HRs and consented to participate. Diagnostic imaging was facilitated using a Chison US equipped with a 10 MH linear probe. The results were adjudged based on the BI-RADS tumor classification.HR markers were discerned through rigorous immunohistochemical analyses. Utilizing SPSS version 21.0, statistical analyses incorporated a variety of tests, including Spearman's rho to assess correlations between hormonal receptors and imaging morphological characteristics and ordinal logistic regression to evaluate the relationships between hormonal receptors and cancer grades. Analyzing the localization of the tumor revealed that a slightly higher proportion had it on the left side, accounting for 52.9% compared to 47.1% on the right side. Regarding the BI-RADS classification observed through echographic examination, a vast majority were classified as BI-RADS 5 (92.8%), followed by a smaller percentage distributed amongst BI-RADS 4 (5.9%), BI-RADS 3 (0.84%), and BIRADS 6 (0.42%). Examining the cancer grades determined that 68.3% were at Grade 2, whereas Grades 1 and 3 were noticeably less common, standing at 1.7% and 30.2%, respectively. Estrogen receptor (ER) and progesterone receptor (PgR) sensitivity were high in most patients, exhibiting 77.7% and 70.6% positivity, respectively, alongside a notable presence of high Ki67 levels in 75.2% of the individuals. The investigation into HER2 status demonstrated that a significant number were negative (76.1%), as opposed to 17.6% being positive and 6.3% equivocal. Remarkably, 5.5% of the patients had a triple-negative status upon biopsy evaluation.The Spearman's rho correlations displayed a moderate positive correlation between ER and PgR (rho=0.563) and a weak negative correlation between ER and Ki67 (rho=-0.343) (P&lt;0.05 in both cases). PgR and Ki67 show a weak negative correlation (rho=-0.353, P&lt;0.05), suggesting a tendency for higher PgR values to correspond with lower Ki67 values. The ordinal logistic regression analysis identified a statistically significant negative relationship between the ER variable and the outcome variable, denoted by a coefficient of -2.137, P&lt;0.05. Additionally, Ki67 showcased a positive relationship with the outcome, as indicated by a coefficient of 5.150, P&lt;0.05. Conclusion: This study delineates the nuanced relationships between biomarkers such as ER, PgR, and Ki67 in different types of infiltrative cancers, pointing to a complex interplay that necessitates further exploration while also noting the independence of BI-RADS imagery in these correlations.