Northern Spain Research Articles

The oldest dairoidid crab (Decapoda, Brachyura, Parthenopoidea) from the Eocene of Spain

AbstractEubrachyurans, or ‘higher’ true crabs, are the most speciose group of decapod crustaceans and have a rich fossil record extending into the Early Cretaceous. However, most extant families are first found in the fossil record in the Palaeogene, and particularly in the Eocene. Unfortunately, fossils of many early eubrachyuran groups are often fragmentary, and only a few studies have combined extinct and extant taxa in a phylogenetic context using different optimality criteria. Here, we report the dairoidid crab Phrynolambrus sagittalis sp. nov., an enigmatic eubrachyuran from the upper Eocene of Huesca (northern Spain), whose completeness and exquisite preservation permit examination of its anatomy in a phylogenetic context. Dairoidids have previously been considered among the oldest stone crabs (Eriphioidea) or elbow crabs (Parthenopoidea), two disparate and distantly related groups of true crabs living today. Mechanical preparation and computed tomography of the fossil material revealed several diagnostic features that allow a detailed comparison with families across the crab tree of life, and test hypotheses about its phylogenetic affinities. Phrynolambrus sagittalis is the first record of the genus in the Iberian Peninsula, and represents one of the oldest crown parthenopoidean crabs worldwide, expanding our knowledge of the biogeographical distribution of elbow crabs during the Palaeogene, as well as their early origins, anatomical diversity and systematic affinities. Understanding the disparity of Eocene eubrachyurans is pivotal to disentangling the systematic relationships among crown families, and interpreting the spatio‐temporal patterns leading to the evolution of modern faunas.

Miren’s Story

Ten-year-old Miren Alonso was one of almost 4,000 Basque children evacuated from the Basque Country in northern Spain to Southampton, England by the Basque Government in May 1937, during the Spanish Civil War (1936-1939). This article, based on extensive oral testimony, provides a firsthand look at a historical event that remains largely unknown to most people. It is also one of only a few long-term studies of the evacuation from the perspective of an individual. This is a summary of Miren’s experience before, during, and after the evacuation, framed within the social, political, and historical context of the period. Her story is a remarkable account of what it was like to be a child refugee, separated from her parents and sent from a war-ravaged city into the care of strangers in a country with a language that she did not speak. It is also the account of what is was like to face the aftereffects of the evacuation and trauma of the Spanish Civil War. This is a story of tragedy on an almost incomprehensible scale, but also a story of resilience, human kindness, and the power of community.

Gamboa's World: Justice, Silver Mining, and Imperial Reform in New Spain

Gamboa's World: Justice, Silver Mining, and Imperial Reform in New Spain

Spatial distribution of hospital admissions for asthma in the central area of Asturias, Northern Spain

BackgroundAsturias is one of the communities with the highest rates of hospital admission for asthma in Spain. The environmental pollution or people lifestyle are some of the factors that contribute to the appearance or aggravation of this illness. The aim of this study was to show the spatial distribution of asthma admissions risks in the central municipalities of Asturias and to analyze the observed spatial patterns.MethodsUrgent hospital admissions for asthma and status asthmaticus occurred between 2016 to 2018 on the public hospitals of the central area of Asturias were used. Population data were assigned in 5 age groups. Standardised admission ratio (SAR), smoothed relative risk (SRR) and posterior risk probability (PP) were calculated for each census tract (CT). A spatial trend analysis was run, a spatial autocorrelation index (Morans I) was calculated and a cluster and outlier analysis (Anselin Local Morans I) was finally performed in order to analyze spatial clusters.ResultsThe total number of hospital urgent asthma admissions during the study period was 2324, 1475 (63.46%) men and 849 (36.56%) women. The municipalities with the highest values of SRR and PP were located on the northwest area: Avilés, Gozón, Carreño, Corvera de Asturias, Castrillón and Illas. A high risk cluster was found for the municipalities of Avilés, Gozón y Corvera de Asturias.ConclusionsThe spatial analysis showed high risk of hospitalization for asthma on the municipalities of the northwest area of the study, which highlight the existence of spatial inequalities on the distribution of urgent hospital admissions.

Genetic Population Flows of Southeast Spain Revealed by STR Analysis

The former Kingdom of Granada, comprising the provinces of Granada, Málaga, and Almería (GMA), was once inhabited for over 700 years (711–1492 AD) by a North African population, which influenced its creation and establishment. The genetic data on 15 autosomal short tandem repeats (STRs) in 245 unrelated donor residents were examined in order to assess any possible admixture. As the two surnames in Spain follow an inheritance similar to the Y chromosome, both surnames of all 245 unrelated individuals were queried and annotated. The Spanish Statistics Office website was consulted to determine the regions with the highest frequency of individuals born bearing each surname. Further, several heraldry and lineage pages were examined to determine the historical origin of the surnames. By AMOVA and STRUCTURE analysis, the populations of the three provinces can be treated genetically as a single population. The analysis of allele frequencies and genetic distance demonstrated that the GMA population lay in the Spanish population group but was slightly more similar to the North African populations than the remainder of the Spanish populations. In addition, the surnames of most individuals originated in Northern and Central Spain, whereas most surnames had higher frequencies in Southern Spain. These results confirm that the GMA population shows no characteristics that reflect a greater genetic influence of North African people than the rest of the populations of the Iberian Peninsula. This feature is consistent with the historical data that African inhabitants were expelled or isolated during the repopulation of the region with Spaniards from Northern Spain. The knowledge of present populations and their genetic history is essential for better statistical results in kinship analyses.

Biases in wind speed measurements due to anemometer changes

This research presents a case study of the biases and discontinuities that were introduced in observed long-term mean wind-speed and gust data-series due to anemometer changes in a meteorological station in northern Spain, operated by the Spanish State Meteorological Agency: San Sebastian-Igueldo. Field and wind-tunnel experiments with predefined conditions have been presented in the literature, however this research uses a real case study to assess the impact of anemometer changes on wind speed measurements due to three factors being: (i) the 3-cup anemometer model (SEAC vs. THIES companies); (ii) sensor height (∼19.95 m vs. ∼20.45 m) and (iii) sensor age (20-years old vs. new). Our results show (a) substantial biases in the measured wind speed and daily peak wind gusts, with the new THIES anemometer reporting stronger surface winds than the old SEAC anemometer; (b) opposing biases under weak (negative) and moderate-strong (positive) winds; and (c) significant breakpoints in the long-term wind data-series, which highlight the importance of data homogenization. National Weather Services and climate assessment groups will benefit from these findings since errors in wind speed and gust measurements can be minimized by implementing systematic observation protocols. Robust anemometer observations provide a basis for accurate quantification of the magnitude of changes and the variability of surface winds.

Teachers' personal network analysis reveals two types of pioneers in educational digitalization: Formal and informal intermediaries at schools

SELFIE is a tool of the European Commission designed to support schools in developing their digital capacity. Schools across Europe have used SELFIE as a diagnostic tool. However, participating schools expressed a need for external guidance in the implementation of digital education transformation, including the exchange of expertise and inspiring practices with other educational centers. In this research we explore what are the key factors in the creation of collaborative networks between teachers. As a case study, we focus on the first steps of building a network of schools to promote digital education in Cantabria (northern Spain). With the participation of 11 schools, we conducted 22 interviews and 2 focus groups to analyze the learning environments of the teaching staff, the personal collaboration networks in the professional field, and the factors perceived as relevant in the design of the digital action plan of each school. The results showed the existence of teachers in each school who were pioneers in the digitization process and who acted as intermediaries both with the public educational administration and with teachers from other schools. During the interviews with these pioneers, we evaluated their personal networks as well as the factors they deem significant for an effective digital transformation. The results were contrasted in a public forum in which 120 teachers participated. Based on these results, we discuss how the creation of a network of key actors could contribute in developing the digital capacity of schools in the region.

Characterization of the thermal death time landscape for Enchytraeus albidus

The influence of non-stressing temperatures on life-history traits of enchytraeids (e.g. growth and reproduction) is well described in the literature, but less is known about the influence of stressful temperatures, especially at the high end of the scale. In light of predicted climate changes, where the frequency and intensity of extreme weather events are increasing, it is important to provide detailed knowledge of the thermal limits of species. Experiments leading to a comprehensive understanding of species´ Thermal Death Time (TDT) landscape are particularly valuable because this allows modelling and predicting mortality under dynamic thermal scenarios. In static assays, we determined TDT50 of adult worms at a range of temperatures showing that 50% mortality (TDT50) was reached by exposure to 35.5 °C for only 6 min, whereas TDT50 at 31 °C was 257 min. By fitting the TDT curve to the measurements, we described the influence of temperature on the rate of injury accumulation leading to 50% mortality. Based on injury accumulation rates derived from static assays, we predicted the TDT50 in dynamic assays of various temperature ramping rates with high precision. Additional experiments showed that eggs and juveniles had the same sensitivity to high temperature as adult worms. Combined with previous research, our results show that E. albidus has a wide thermal niche in which survival is possible, from ca. − 25 to + 35 °C. However, exposure time is of the essence for surviving stressful temperatures in the high and low temperature ranges. We discuss that the wide thermal niche of E. albidus may partly explain how this species has become so widely distributed, from the temperate coastal climate in northern Spain to the high Arctic in Svalbard.

Geomorphological evolution and mapping of the littoral of Asturias and Cantabria (Northern Spain) in the area of El Pindal Cave: relations between coastline and karstic morphologies

ABSTRACT Along the northern littoral of the Iberian Peninsula, from Cuerres (Asturias) to Oyambre (Cantabria) there are caves, such as El Pindal (World Heritage List). Two geomorphologic maps have been prepared: a general scaled 1:25000, and a detail one of the cave, with erosional and depositional landforms geomorphologic units. A relative Quaternary chronology for the depositional landforms, uses colour spots. Marine morphologies suitable for determining past sea-level positions were surveyed to identify the elevation of past high-stands and reconstructing paleo-shorelines. As a result, the study of seven erosional surfaces (rasas) in quartzites and six on limestones led to reconstructing a general sequence between 275–120 m and 90–1 m above sea level (a.s.l.) respectively. Around the El Pindal cave there are four rasas cut on quartzites (171–160 m) and four cut on limestone (65–32 m). Other additional indicators of paleoshorelines are: 24–25 m entrance to the El Pindal cave, 20–21 m lower Pindal, 15–17 m marine terrace, 9–10 m shelter, 2–3 m notches and 0–1 m present rasa.Considering the elevation rates for the various rasas, an estimated value of 0.066 mm/y as the most probable, it is concluded that the beginning of Quaternary lays at 172 m a.s.l.

Energy regimes help tackle limitations with the prehistoric cultural‐phases approach to learn about sustainable transitions: Archaeological evidence from northern Spain

ABSTRACTHuman societies face challenges in transitioning towards low‐carbon economies and sustainable management of land use and natural resources. Documenting and learning from past transitions helps policy‐makers cope with such challenges. The agricultural revolution in Cantabrian Spain (ca. 7000 cal a bp) was one major adaptation of hunter‐gatherers to a changing environment that started with the Last Glacial Maximum (ca. 24 000 cal a bp) and lasted until the Mid‐Holocene (ca. 5300 cal a bp). Classic approaches to documenting prehistoric cultural timelines are based on manufacturing and technology, thus limited in their ability to describe the sustainability of past societies. Energy regimes, a functional societal approach independent from time, investigate and consider patterns of resource and energy use in various cohabiting and cooperating cultural phases. To examine past energy regimes, a database of archaeological remains was compiled to document four indicators: mobility, economy, overexploitation and societal complexity. Statistical analyses were conducted to elucidate trends, changes and continuity in subsistence strategies by hunter‐gatherers and sedentary societies. Results show that energy regimes act as a complement to cultural phases, adding novel functional analyses of past societies to cultural stratigraphy units common in archaeology, shedding light on the sustainability of past societal transitions.

Impact of COVID-19 restrictions on hourly levels of PM10, PM2.5 and black carbon at an industrial suburban site in northern Spain

Due to the COVID-19 pandemic, lockdown restrictions were established around the world. Many studies have assessed whether these restrictions affected atmospheric pollution. Comparison between them is difficult as the periods of time considered are generally not the same and thus, different conclusions may be reached. Besides, most of them consider mean daily pollutant concentration, despite differences being observed according to the time of day. In this study, the hourly levels of PM10, PM2.5 and black carbon (BC) in an industrial suburban area in the north of Spain were analysed from May 2019 to June 2020 and compared with those from the literature, using the same period in each case. In general, the highest concentrations were reached when the wind direction came from the southwest (where a steelworks, a coal-fired power plant and other industries are located) and during the night-time, both before and during the lockdown. The highest concentrations of PM10, PM2.5 and BC were observed from December to February (on average: 45, 17 and 1.3 μg m−3, respectively). The decrease/increase in those pollutants levels during the lockdown were found to be highly dependent on the period considered. Indeed, PM10 can be found to decrease by up to 39% or increase by 12%; PM2.5 can decrease by 21% or increase by up to 36%; and BC, although it generally decreases (by up to 42%), can increase by 7.4%.

Soil and Land Cover Interrelationships: An Analysis Based on the Jenny’s Equation

This research analyzes the relationships between “soil” and “organisms” within the framework of the Jenny equation, a fundamental expression in soil science that is the theoretical basis for modeling the complex occurrence of soils on landscapes. This analysis is based on the interpretation of the indeterminate function “f” of the equation as “statistical dependence between categorical variables”. The categories of the “soil” component of the equation have been defined as “diagnostic horizons”, and those of the “organisms” factor as synthetic types of “land cover”. After applying these criteria to 424 soil profiles studied in a region with an oceanic climate in northern Spain, a multiple correspondence analysis showed pedologically consistent groupings between diagnostic horizons and categories of climate, land cover, relief, and parent material factors. Subsequently, a bivariate analysis detailed pedologically consistent relationships between diagnostic horizons and land cover categories. In the context the scarcity of quantitative information on soil and forming factor relationships, this work provides criteria to statistically assess the role of land cover in such relationships. This soil forming factor is the one whose spatial representation is more generalized and detailed, hence its interest in the development of soil mapping models.

Holocene environmental evolution and relative sea-level change in the Oka estuary (Urdaibai Biosphere Reserve, northern Spain)

The Holocene environmental evolution in coastal areas, including deltas and estuaries, is vital to understanding coastal dynamics and how they may change in the future. Here, we studied the Holocene environmental evolution of the Oka estuary (Urdaibai Biosphere Reserve, northern Spain) from sedimentary cores that were 11–49 m long. We applied a multi-proxy approach of benthic foraminifera and grain size, and a temporal framework of radiocarbon dates to reconstruct paleoenvironments. The benthic foraminifera assemblages varied from near-marine (allochthonous and autochthonous hyaline species such as Lobatula lobatula, Rosalina irregularis, Ammonia tepida and Haynesina germanica) to brackish intertidal (autochthonous hyaline species such as A. tepida and H. germanica) to salt-marsh (autochthonous agglutinated species Trochammina inflata and Entzia macrescens) environments. Grain size analysis supported the foraminiferal assemblages and the sedimentary sequences were sand-dominated in near-marine intertidal environments and mud-dominated in the brackish intertidal and salt-marsh environments. The chronology was constrained by thirty four radiocarbon dates from plant macrofossils, marine shells and wood fragments. Our paleoenvironmental reconstructions were used to produce an updated local Holocene relative sea-level (RSL) record based on new data, revised reconstructions, and age recalibration of previous research. Marine limiting dates showed that RSL changed from above −20.4 ± 0.2 m at ∼9100 cal. yrs. BP to above −4.2 ± 0.2 m at ∼6700 cal. yrs. BP. In the late Holocene, sea-level index points (SLIPs) reconstructed the RSL position from −2.7 ± 1.2 m at ∼4900 cal. yrs. BP to −1.4 ± 1.2 m at ∼1500 cal. yrs. BP. Estimates of rates of RSL change from the Errors-in-Variables Integrated Gaussian Process (EIV-IGP) model show RSL gradually rose at 0.3 ± 2.1 mm yr−1 in the late Holocene. There was a progressive shallowing of the estuary as rates of RSL rise reduced during the Holocene. Between ∼9000 and 7000-6000 cal. yrs. BP pre-Holocene fluvial gravels (Lowstand Systems Tract) were replaced by both sandy marine and brackish intertidal sediments (Transgressive Systems Tract). After ∼7000-6000 cal. yrs. BP salt-marsh sediments deposited (Highstand Systems Tract). The sequence was overlain by anthropogenic deposits derived from agricultural, dredging and dumping activities since the 18th century (Anthropogenic Systems Tract). The Holocene environmental evolution and relative sea-level change of Oka is broadly consistent with previous studies in northern Spain, although it places the onset of the Highstand Systems Tract ∼2000 years earlier.

Potential of TROPOMI for understanding spatio-temporal variations in surface NO2 and their dependencies upon land use over the Iberian Peninsula

Abstract. In orbit since late 2017, the Tropospheric Monitoring Instrument (TROPOMI) is offering new outstanding opportunities for better understanding the emission and fate of nitrogen dioxide (NO2) pollution in the troposphere. In this study, we provide a comprehensive analysis of the spatio-temporal variability of TROPOMI NO2 tropospheric columns (TrC-NO2) over the Iberian Peninsula during 2018–2021, considering the recently developed Product Algorithm Laboratory (PAL) product. We complement our analysis with estimates of NOx anthropogenic and natural soil emissions. Closely related to cloud cover, the data availability of TROPOMI observations ranges from 30 %–45 % during April and November to 70 %–80 % during summertime, with strong variations between northern and southern Spain. Strongest TrC-NO2 hotspots are located over Madrid and Barcelona, while TrC-NO2 enhancements are also observed along international maritime routes close the strait of Gibraltar, and to a lesser extent along specific major highways. TROPOMI TrC-NO2 appear reasonably well correlated with collocated surface NO2 mixing ratios, with correlations around 0.7–0.8 depending on the averaging time. We investigate the changes of weekly and monthly variability of TROPOMI TrC-NO2 depending on the urban cover fraction. Weekly profiles show a reduction of TrC-NO2 during the weekend ranging from −10 % to −40 % from least to most urbanized areas, in reasonable agreement with surface NO2. In the largest agglomerations like Madrid or Barcelona, this weekend effect peaks not in the city center but in specific suburban areas/cities, suggesting a larger relative contribution of commuting to total NOx anthropogenic emissions. The TROPOMI TrC-NO2 monthly variability also strongly varies with the level of urbanization, with monthly differences relative to annual mean ranging from −40 % in summer to +60 % in winter in the most urbanized areas, and from −10 % to +20 % in the least urbanized areas. When focusing on agricultural areas, TROPOMI observations depict an enhancement in June–July that could come from natural soil NO emissions. Some specific analysis of surface NO2 observations in Madrid show that the relatively sharp NO2 minimum used to occur in August (drop of road transport during holidays) has now evolved into a much broader minimum partly de-coupled from the observed local road traffic counting; this change started in 2018, thus before the COVID-19 outbreak. Over 2019–2021, a reasonable consistency of the inter-annual variability of NO2 is also found between both datasets. Our study illustrates the strong potential of TROPOMI TrC-NO2 observations for complementing the existing surface NO2 monitoring stations, especially in the poorly covered rural and maritime areas where NOx can play a key role, notably for the production of tropospheric O3.

Ascaridoid parasites in European sardine throughout the annual cycle: Variability in parasitic load according to host stock features.

In recent years, a drop in the condition of the European sardine has been observed. Although several causes have been attributed to this issue, as overfishing and climate change, little is known about the link between ascaridoid nematode parasitisation and fish status. In this study, sardines were obtained from four fishing grounds along the Mediterranean (Alboran, Northern Spain, Northern Adriatic, and Aegean), and one location in the Atlantic Ocean (Southern Portugal). After analysing individual fish body condition (by direct tissue fat content measurements and condition indices), and reproductive status (by a detailed gonadal examination) throughout the entire annual cycle, ascaridoids were recognised by combining naked eye and UV-press method along flesh, viscera, and gonads. Afterwards, sequence analysis of the rDNA internal transcribed spacers region (ITS) and the mtDNA cox2 gene were used to identify and characterise the different species of ascaridoids from the fish host in the localities throughout the seasons. The main species found along different areas was Hysterothylacium aduncum, present in the Northern Adriatic (prevalence of 7.6%, mean intensity 1.700), the Atlantic (7.5%, 3.889), and the Northern Spain (3.9%, 1.600). Moreover, few individuals of Anisakis simplex (s.s.) and A. pegreffii were observed in the Atlantic (1.7% and 0.8%, respectively), and the latter species was also found in the Adriatic stock (0.8%). All ascaridoid specimens were found in viscera. Obtained results seem to indicate that in stocks with medium sizes, small variations in length are related to parasite intensity. This study highlights the importance of seasonal parasitological analyses at stock level and, especially, in capital breeders, as relationships between condition and reproduction parameters and parasitism are conditioned by seasonality.

What Hides in the Heights? The Case of the Iberian Endemism Bromus picoeuropeanus

Bromus picoeuropeanus is a recently described species belonging to a complex genus of grasses. It inhabits stony soils at heights ranging from 1600 to 2200 m in Picos de Europa (Cantabrian Mountains, northern Spain). This species is morphologically very similar to B. erectus, partially sharing its presumed distribution range. We aim to determine the relationship between these species and their altitudinal ranges in Picos de Europa and the Cantabrian Mountains by conducting phylogenetic analyses based on nuclear (ETS and ITS) and chloroplastic (trnL) markers. Phylogenetic trees were inferred by Maximum Likelihood and Bayesian Inference. Haplotype networks were estimated based on the plastid marker. Although the ITS topologies could not generate exclusive clades for these species, the ETS analyses generated highly supported B. picoeuropeanus exclusive clades, which included locations outside its altitudinal putative range. The ETS-ITS and ETS-ITS-trnL topologies generated B. picoeuropeanus exclusive clades, whereas the trnL-based trees and haplotype networks were unable to discriminate B. erectus and B. picoeuropeanus. This evidence suggests that B. picoeuropeanus is a separate species with a larger distribution than previously thought, opening new questions regarding the evolution of B. erectus and other similar species in European mountainous systems. However, more information is needed regarding B. picoeuropeanus susceptibility to temperature rises.

(2946) Proposal to conserve the name Iris latifolia (Mill.) Voss (Iridaceae) against I. latifolia Heer (fossil Iridaceae)

(2946) Proposal to conserve the name <i>Iris latifolia</i> (Mill.) Voss (<i>Iridaceae</i>) against <i>I. latifolia</i> Heer (fossil <i>Iridaceae</i>)

Lower Cenomanian (Cretaceous) corals from Cóbreces (Cantabria, northern Spain). Part 2: family Latomeandridae

Lower Cenomanian (Cretaceous) corals from Cóbreces (Cantabria, northern Spain). Part 2: family Latomeandridae

Description of a Cohort with a New Truncating MYBPC3 Variant for Hypertrophic Cardiomyopathy in Northern Spain

The pathogenicity of the different genetic variants causing hypertrophic cardiomyopathy (HCM) and the genotype/phenotype correlations are difficult to assess in clinical practice, as most mutations are unique or identified in non-informative families. Pathogenic variants in the sarcomeric gene MYBPC3 inherited with an autosomal dominant pattern, whereas incomplete and age-dependent penetrance are the most common causes of HCM. We describe the clinical characteristics of a new truncating MYBPC3 variant, p.Val931Glyfs*120, in 75 subjects from 18 different families from northern Spain with the p.Val931Glyfs*120 variant. Our cohort allows us to estimate the penetrance and prognosis of this variant. The penetrance of the disease increases with age, whereas 50% of males in our sample developed HCM by the age of 36 years old, and 50% of women developed the disease by the time they reached 48 years of age (p = 0.104). Men have more documented arrhythmias with potential risk of sudden death (p = 0.018), requiring implantation of cardioverter defibrillators (p = 0.024). Semi-professional/competitive sport among males is related to earlier onset of HCM (p = 0.004). The p.Val931Glyfs*120 truncating variant in MYBPC3 is associated with a moderate phenotype of HCM, with a high penetrance, onset in middle age, and a worse outcome in males due to higher risk of sudden death due to arrhythmias.

Soil Properties of Abandoned Vineyards

The abandonment of vineyards has shown to change the soil properties and structure. The analysis of vineyards in the Castilla y Leon region in northern Spain and the soil properties of specific points in the regions demonstrate that there is a change after abandonment of vineyard land. Historical data has shown that there has been observed changes in the soil and the hydrological properties in the soil after a vineyard has been abandoned (Vazquez-Blanco, 2022). Comparisons made between active vineyards and those that have been abandoned show some contrasts between each in the collected soil data and how the land use and land cover has changed (ESDAC, 2022). Additionally, the determination of why the vineyards were abandoned by the production yield and if the vineyards were abandoned due to economic reasons or if climatic changes caused for the land to be deem unsuitable for vineyard occupation. The purpose of this report is to show that soil properties and soil structure is altered in an area after vineyards are abandoned and the land is no longer cultivated. The comparisons of active vineyards and vineyards that are now abandoned provide for the stark differences to be observed.