Northern Spain Research Articles

Genetic Diversity of Lecanosticta acicola in Pinus Ecosystems in Northern Spain.

Lecanosticta acicola is one of the most damaging species affecting Pinus radiata plantations in Spain. Favourable climatic conditions and unknown endogenous factors of the pathogen and host led to a situation of high incidence and severity of the disease in these ecosystems. With the main aim of understanding the factors intrinsic to this pathogenic species, a study of the population structure in new established plantations with respect to older plantations was implemented. The genetic diversity, population structure and the ability of the pathogen to spread was determined in Northern Spain (Basque Country), where two thirds of the total Pinus radiata plantations of Spain are located. From a total of 153 Lecanosticta acicola isolates analysed, two lineages were present; the southern lineage, which was prevalent, and the northern lineage, which was scarce. A total of 22 multilocus genotypes were detected with a balanced composition of both mating types and evidence for sexual reproduction. In addition to the changing environmental conditions enhancing disease expression, the complexity and diversity of the pathogen will make it difficult to control and to maintain the wood productive system fundamentally based on this forest species.

Method for the Detection of Functional Outliers Applied to Quality Monitoring Samples in the Vicinity of El Musel Seaport in the Metropolitan Area of Gijón (Northern Spain)

Air pollution affects human health and is one of the main problems in the world, including in coastal cities with industrial seaports. In this sense, the city of Gijón (northern Spain) stands out as one of the 20 Spanish cities with the worst air quality. The study aims to identify outliers in air quality observations near the El Musel seaport, resulting from the emissions of six pollutants over an eight-year period (2014–2021). It compares methods based on the functional data analysis (FDA) approach and vector methods to determine the optimal approach for detecting outliers and supporting air quality control. Our approach involves analyzing air pollutant observations as a set of curves rather than vectors. Therefore, in the FDA approach, curves are constructed to provide the best fit to isolated data points, resulting in a collection of continuous functions. These functions capture the behavior of the data in a continuous domain. Two FDA approach methodologies were used here: the functional bagplot and the high-density region (HDR) boxplot. Finally, outlier detection using the FDA approach was found to be more powerful than the vector methods and the functional bagplot method detected more outliers than the HDR boxplot.

Warming overrides eutrophication effects on leaf litter decomposition in stream microcosms

Several human activities often result in increased nitrogen (N) and phosphorus (P) inputs to running waters through runoff. Although headwater streams are less frequently affected by these inputs than downstream reaches, the joint effects of moderate eutrophication and global warming can affect the functioning of these ecosystems, which represent two thirds of total river length and thus are of major global relevance. In a microcosm study representing streams from a temperate area (northern Spain), we assessed the combined effects of increased water temperature (10.0, 12.5, and 15.0 °C) and nutrient enrichment (control, high N, high P, and high N + P concentrations) on the key process of leaf litter decomposition (mediated by microorganisms and detritivores) and associated changes in different biological compartments (leaf litter, aquatic hyphomycetes and detritivores). While warming consistently enhanced decomposition rates and associated variables (leaf litter microbial conditioning, aquatic hyphomycete sporulation rate and taxon richness, and detritivore growth and nutrient contents), effects of eutrophication were weaker and more variable: P addition inhibited decomposition, addition of N + P promoted leaf litter conditioning, and detritivore stoichiometry was affected by the addition of both nutrients separately or together. In only a few cases (variables related to detritivore performance, but not microbial performance or leaf litter decomposition) we found interactions between warming and eutrophication, which contrasts with other experiments reporting synergistic effects. Our results suggest that both stressors can importantly alter the functioning of stream ecosystems even when occurring in isolation, although non-additive effects should not be neglected and might require exploring an array of ecosystem processes (not just leaf litter decomposition) in order to be detected.

Yield, Chemical Composition and Bioactivity of Essential Oils from Common Juniper (Juniperus communis L.) from Different Spanish Origins.

Essential oils (EOs) obtained from Juniperus communis L. are frequently used in the production of bioproducts. However, there are no studies regarding industrial crops' production, allowing for better control of the quality and production of juniper EOs. To select the plant material for developing future crops of this species in northern Spain, four locations where this shrub species grows in the wild were selected and samples of both genera were collected. The EOs were obtained by steam distillation, and their chemical composition and bioactivity were evaluated. The results showed that the yield of EOs from male and female samples were within the usual reported ranges, varying between 0.24 and 0.58% (dry basis, d.b.). However, limonene content in three locations varied between 15 and 25%, which is between 100% and 200% higher than the values usually reported for other European countries. The antibacterial activity was determined by broth microdilution and showed that gram-positive bacteria were more susceptible to the tested EOs since, in general, lower MIC values were obtained compared to gram-negatives. The EOs from location 1 (L1F) and 2 (L2M) inhibited the growth of six out of the eight clinical strains tested. Samples from location 1 were particularly effective, exhibiting MBC against two gram-negative (E. coli and P. mirabilis) and one gram-positive bacteria (E. faecalis). Moreover, the majority of the EOs tested showed anti-inflammatory activity. Cytotoxic effect has been demonstrated in tumor cell lines, with the best results observed against gastric carcinoma (AGS) cells (GI50 between 7 to 77 µg/mL). Although generally presenting higher GI50, most samples also inhibited the growth of non-tumoral cells, particularly hepatocytes (PLP2 cells). Therefore, its use for their anti-proliferative activity should consider specific conditions to avoid damaging normal cells. Finally, the results and conclusions obtained led to the selection of the female shrubs from location 1 (L1F) as the plant material to be propagated in order to produce plants for a future juniper crop.

Health Security Perceptions And Length of Stay

The health risks associated with COVID-19 have increased tourists' safety concerns when traveling. We analyze the linkages between tourists' length of stay and their perceptions about health security in different public venues at the destination: the street, bars and cafés, restaurants, the chosen accommodation, the beach, and shops and stores. We use survey data collected from tourists to a nature-based region (Asturias) in Northern Spain in the summer of 2020 as our case study. Based on separate linear regressions, we examine how health security perceptions vary with the length of the stay across different public venues. In doing so, we control for a wide set of sociodemographic and trip-related characteristics. We find evidence of a consistent nonlinear negative relationship between the length of the stay and the perceived health security in these public venues. We also document that hotel guests have lower health security perceptions, both at the hotels themselves and other public venues. This is the first work that explores how health security perceptions in different public domains deteriorate with the tourists' length of stay.

Meso‐habitat distribution patterns and ecological requirements of two Mediterranean vipers depict weak competition in a contact zone

AbstractDisentangling the multiple factors that shape the ecological niche of a species is often difficult, especially when in contact with other similar and potentially competing species. Ecological niche models (ENMs), albeit originally developed for environmental factors only, can help in assessing competition by comparing the niches of ecologically similar species. Here, we use ENMs and niche overlap tests to compare the ecological niches of the Western Mediterranean vipers, Vipera aspis and V. latastei, in the secondary contact zone of the High Ebro (Northern Spain), partitioning the species occurrences in sympatric and allopatric subsets. We considered nine geomorphological and landcover variables, obtained from remote sensing, and gathered at the meso‐habitat scale (100 × 100 m). Our ENMs showed partial interspecific divergence in habitat requirements between these two closely related species, with V. aspis selecting more canopied areas and V. latastei preferring more open habitats. No intraspecific differences in ecological requirements were found when sympatric and allopatric subsets were compared, that is, the two species are not experiencing ecological shift when in contact, which suggests weak competition in habitat use. Our findings, therefore, suggest that species distributions in this contact zone are mainly determined by the environmental conditions, with almost no contribution of competitive pressure. Still, a localized competition might exist due to minor differences in the ecological niches and a marked and unequal reduction of suitable areas for the sympatric populations, which could be favouring V. aspis in small regions of the contact zone.

Socioeconomic, Technological, and Cultural Adaptation of the Mesolithic Population in Central-Eastern Cantabria (Spain) in the Early and Middle Holocene

Abstract This article presents the research on the palaeoenvironmental changes that took place at the end of the Tardiglacial, in the early and middle Holocene, observed in sites of anthropic origin in central-eastern Cantabria. Through the comparative analysis of the economy, the settlement pattern, and the industries of the Azilian and Mesolithic settlements, we try to infer the repercussions they could have had on the last hunter-gatherers, in order to adapt to the modification of the territory, the change in the available resources, and the socio-economic consequences they could have had on the settlement. The radiocarbon record of central-eastern Cantabria and the Cantabrian region (Northern Spain) between 9.5 and 7.9 ka cal BP is analysed in order to assess the impact of the cold events that occurred in 9.3 and 8.2 ka cal BP, identified in the palaeoenvironment.

Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA

The European eel Anguilla anguilla is a critically endangered catadromous species. There is an urgent need for close surveillance of the populations that are still viable in European rivers. The species is difficult to observe in freshwater because of its bottom-dwelling behavior; the currently employed methods of eel monitoring in Europe based on the physical capture of individuals are stressful and may cause mortality. Here, we present a new highly sensitive method based on an A. anguilla-specific qPCR marker designed within the cytochrome oxidase I mitochondrial gene for application on environmental DNA (eDNA). Since the detectability of eDNA depends on the hydrographic conditions, we applied correction for altitude and a linear model and were able to predict the eel biomass from the eDNA in the different rivers of northern Spain still holding wild populations. The method was validated by electrofishing surveys. This novel eDNA-based marker allows for estimating the European eel biomass in running waters from small 1.5 L water samples and could complement, or replace in some cases, current eel surveys without disturbing wild populations.

Minimum average pack size in Iberian wolves

We present information on the minimum average number of wolves per pack in late summer derived from fieldwork activities during the last three decades in north-western Spain, and on the mean minimum number of wolves travelling together in winter in the Cantabrian Mountains. The minimum average litter size observed between August and October (when the pups are between 3–6 months old) was 5.15 (CI 95%: 4.50–5.85); whereas the minimum mean number of adults/subadults observed was 3.85 (CI 95%: 3.30–4.45). Combined, the estimated minimum average pack size in late summer was 8.95 wolves (CI 95%: 8.10–9.80). In winter time (December-March), the mean number of wolves observed travelling together was 3.44 (CI 95%: 3.23–3.68). When using the average minimum pack size to convert wolf reproductions into an estimated number of wolves in late summer, an estimated number of wolves not linked to breeding packs should be also considered, which, in northern Spain, has been estimated between 16 and 25% of the total number of wolves at this time of the year.

Were there missing girls in Biscay? Sex ratios in Northern Spain, 1550-1899

This paper seeks to contribute to the knowledge of sex ratios at birth, their long-term evolution, and their determinants, on a sound documentary base of almost one million records between 1550 and 1890. The region studied likewise offers the advantage of being industrialised at the end of the observation period, which allows relevant conclusions to be drawn about the effect of modern economic growth on family decisions regarding their offspring. The main conclusions include the preference for males in the rural world, favoured by the inheritance regime and the scarce ecclesiastical regulation in this territory, while the city levels were similar to those of other European cities. From the 18th century onwards, a stability around the reference values in allareas could be observed and which was only broken by the economic and social changes during industrialisation.

Artisan entrepreneurship, resilience and sustainable development: the quintuple helix innovation model in the low-density and cross-border territories

PurposeThe aim of this study is, from the perspective of artisan entrepreneurship, to trace and analyse the artisan's profile in the sustainable development of low population density cross-border territories, using the quintuple helix innovation model.Design/methodology/approachTen semi-structured interviews were conducted with cultural and traditional artisans to achieve the proposed objective using a qualitative approach. The artisans are from Northeast Portugal (Bragança, Miranda do Douro, Mogadouro, Vimioso and Vinhais) and Northern Spain (Province of Zamora). The interviews were conducted face-to-face between May and June 2022. The interviews were manually transcribed and subjected to content and lexical analyses using IRaMuTeQ software.FindingsAn artisan was identified as an enterprising individual whose marketed handicraft pieces transmit the values and teachings of his community. Innovation, technology, sustainability and circular economy in a family environment, where dedication, resilience, happiness and hard work transmit an identity that places the artisan and his artisan practice as the driving force for the enhancement and promotion of his territory, cultural heritage and identity.Originality/valueThis work is the first study to address and treat the issue of artisan entrepreneurship by analysing and defining the cultural-based and traditional artisan profile in a cross-border and low population density territorial context.

Health-Related Quality of Life in Pregnant Women during the First Trimester in Northern Spain: A Descriptive Cross-Sectional Study.

Achieving the optimal quality of life is currently a health challenge for the world's population. Pregnancy is a stressful period of life that affects women's quality of life. This study aimed to describe and analyse the health-related quality of life in pregnant women during their first trimester in a health area in the north of Spain. A cross-sectional descriptive study was carried out. A total of 359 women completed the 36-Item Short-Form Health Survey. The sample consisted of 57.9% primiparous women, 30% had experienced a previous abortion, and 7.2% were foreign women. The mean age was 33.53 years. The sum of the physical and mental component values was below 50 points. Notably, 4.17% of women reported a worsening of their health in the last year, and 28.69% had an increased depression risk. Being a foreigner, prenatal abortion, previous caesarean section, previous children, or assisted reproduction techniques are the variables that have a negative association with some dimensions of quality of life in pregnant women.

Assessing Thermal Comfort Perception in the Context of Social Housing. Case Study in Northern Spain

The influence of people on building performance in terms of energy efficiency and environmental impact is becoming increasingly significant. It is essential to include users’ perspective, their comfort and satisfaction in decision making to ensure not only their well-being, but also the feasibility of interventions and the adequate performance of the building stock. Furthermore, understanding residents’ level of thermal satisfaction can enable more appropriate measures to improve the energy efficiency of buildings. Although there are several methods for studying thermal comfort, such as qualitative analyses based on surveys or perceived comfort indices such as PPD and PMV, thermal satisfaction is susceptible to the subjectivity of the responses. It may be necessary to contrast different indices or methods. This study aims to define an indicator that measures the level of thermal satisfaction of social housing occupants so that it can be contrasted with other methods of analysis of perceived comfort and can be replicated in different building contexts. A way to analyse users’ thermal satisfaction is proposed in a quantitative way, measured as the difference of the desired temperature and the perceived indoor temperature. The index is applied to a sample of 283 social housing dwellings in the Basque Country, Spain, with the data obtained via surveys that include questions on thermal comfort in winter and households’ characteristics. The thermal satisfaction has been analysed and the results have been contrasted with the perceived thermal comfort in winter and the household’s capacity to maintain the dwelling at the desired temperature. Moreover, it has been observed whether there may be energy vulnerabilities by contrasting the satisfaction result with the income and expenditure per person in the household. The obtained variable provides occupants’ opinion and perception to ensure the suitability of the solutions for improving the energy efficiency of the building and the thermal comfort. It is also possible to apply it to different building typologies and compare the results with other models of perceived thermal comfort.

The Eurasian shrew and vole tick Ixodes trianguliceps: geographical distribution, climate preference, and pathogens detected

The Eurasian shrew and vole tick Ixodes trianguliceps Birula lives in the nests and burrows of its small mammalian hosts and is—along with larvae and nymphs of Ixodes ricinus or Ixodes persulcatus—one of the most commonly collected tick species from these hosts in its Eurasian range. Ixodes trianguliceps is a proven vector of Babesia microti. In this study, up-to-date maps depicting the geographical distribution and the climate preference of I. trianguliceps are presented. A dataset was compiled, resulting in 1161 georeferenced locations in Eurasia. This data set covers the entire range of I. trianguliceps for the first time. The distribution area between 8^circ W–105^circ E and 40–69^circ N extends from Northern Spain to Western Siberia. To investigate the climate adaptation of I. trianguliceps, the georeferenced locations were superimposed on a high-resolution map of the Köppen–Geiger climate classification. The Köppen profile for I. trianguliceps, i.e., a frequency distribution of the tick occurrence under different climates, shows two peaks related to the following climates: warm temperate with precipitation all year round (Cfb), and boreal with warm or cold summers and precipitation all year round (Dfb, Dfc). Almost 97% of all known I. trianguliceps locations are related to these climates. Thus, I. trianguliceps prefers climates with warm or cold summers without dry periods. Cold winters do not limit the distribution of this nidicolous tick species, which has been recorded in the European Alps and the Caucasus Mountains up to altitudes of 2400 m. Conversely, I. trianguliceps does not occur in the Mediterranean area with its hot and dry summers.

Pentobarbital intoxication as a potential underlying cause for electrocution in a bearded vulture.

A bearded vulture (Gypaetus barbatus) found dead in northern Spain presented external lesions consistent with electrocution as the cause of death. During forensic examination, macroscopic lesions suggested potential comorbidity, so samples were collected for molecular and toxicological analyses. Gastric content and liver were analysed for toxic substances, and pentobarbital (a common pharmaceutical used for euthanasia in domestic animals) was detected at a concentration of 37.3 and 0.05µg/g, respectively. Other toxicological, viral and endoparasite analyses (avian malaria, avian influenza and flaviviruses) were negative. Thus, although the cause of death was electrocution, pentobarbital intoxication likely impaired the equilibrium and reflexes of the individual, possibly causing the bird to contact energized wires that it would not have otherwise. These results underline the importance of comprehensive analysis of forensic cases of wildlife deaths and reveal barbiturate poisoning as an additional threat for the conservation of the bearded vulture in Europe.

Capturing technological crossovers between clay crafts: An archaeometric perspective on the emergence of workshop production in Late Iron Age northern Spain.

In the Iberian Iron Age, the transition to workshop-based pottery production involved the use of innovative tools (the potter's wheel and kiln) and dedicated workspace. This facilitated an intensification of production, with repercussions for consumption practices and the economy. Cross-craft comparison can contribute to understanding the transmission processes underpinning this transition, as well as its impact on local craft traditions. This paper discusses an archaeometric methodology to compare the technological procedures underpinning different clay crafts to reveal crossovers and divergences that are meaningful for understanding cross craft interaction and the spread of technological innovations. We use thin-section ceramic petrography, X-Ray Fluorescence, Inductively Coupled Plasma-Mass Spectrometry, and X-Ray Diffraction to analyse the mineralogical and geochemical compositions and levels of standardisation in hand-made pottery, wheel-made ceramics, and ceramic building materials from the Late Iron Age oppidum of Monte Bernorio (Aguilar de Campoo, Palencia) and the kiln site of El Cerrito (Cella, Teruel). The results demonstrate that wheel-made pottery was produced according to a highly uniform clay preparation and clay selection procedure, which spanned the northern Iberian Plateau and largely existed in isolation from local pottery traditions. At Monte Bernorio, wheel-made pottery was made on-site from non-local clays, suggesting that suitable clays were brought to the site, perhaps by itinerant potters working on a seasonal basis. Technological traditions were thus largely polarised, demonstrating that knowledge, skills, and markets relating to workshop-produced pottery were enacted by a segment of society operating as part of a closed technological system.

Molecular characterization of a suspected IMP-type carbapenemase-producing Pseudomonas aeruginosa outbreak reveals two simultaneous outbreaks in a tertiary-care hospital.

To describe IMP-type carbapenemase-producing Pseudomonas aeruginosa outbreaks at Galdakao University Hospital between March 2021 to December 2021. Outbreak report. Galdakao University Hospital is a tertiary-care hospital in the Basque Country (northern Spain). All patients with a positive IMP-type carbapenemase producingPseudomonas aeruginosa (IMP-PA) culture were included in this study, both colonization and infection cases. An outbreak investigation was conducted, in which molecular epidemiology analysis [pulsed-field gel electrophoresis and whole-genome sequencing (WGS)] and environmental screenings were performed. Between March and December 2021, 21 cases of IMP-PA were detected in Galdakao University Hospital: 18 infection cases and 3 colonization cases. In total, 4 different pulsotypes were detected belonging to 4 clones according to WGS: ST175 (n = 14), ST633 (n = 3), ST179 (n = 3), and ST348 (n = 1). IMP-13 was detected in most isolates belonging to the ST175 clone and in all ST179 and ST348 clones, whereas IMP-29 was detected in isolates belonging to the ST633 clone. Clinical isolates belonging to the ST175 clone were isolated mainly from patients admitted to the respiratory ward, and isolates belonging to the ST633 clone from patients admitted to the ICU. Two environmental isolates belonging to the ST175 clone were detected in the respiratory ward. Molecular and genomic epidemiology revealed that there had been 2 independent IMP-PA outbreaks, one of long duration in the respiratory ward and the other more limited in the ICU.

Mosquito community composition in two major stopover aquatic ecosystems used by migratory birds in northern Spain.

Mosquitoes (Diptera: Culicidae) are common bloodsucking Diptera frequently found in aquatic environments, which are valuable ecosystems for many animal species, particularly migrating birds. Therefore, interactions between these animal species and mosquitoes may play a critical role in pathogen transmission. During 2018-2019, mosquitoes were collected from two aquatic ecosystems in northern Spain using different methodologies and identified using classical morphology and molecular tools. A total of 1529 males and females of 22 native mosquito species (including eight new records for the region) were trapped using CO2 -baited Centers for Disease Control and Prevention (CDC) traps and sweep netting. Among the blood-fed female mosquitoes, 11 vertebrate host species-six mammals and five birds-were identified using DNA barcoding. The developmental sites of eight mosquito species were determined across nine microhabitats, and 11 mosquito species were caught landing on humans. The flight period varied among mosquito species, with some peaking in the spring and others in the summer. Our study highlights the advantages of mosquito sampling using various techniques to comprehensively characterise species composition and abundance. Information on the trophic preferences, biting behaviour and influence of climatic variables on the ecology of mosquitoes is also provided.

Auxins seem promising as a tuning method for balancing sugars with acidity in grape musts from cv. Tempranillo, but not defoliation or application of magnesium to leaves

Global warming boosted by climate change affects grape quality, with increasing total soluble solids (TSS) content and decreasing total acidity (TA). However, current wine preferences increasingly include moderate alcohol content, higher acidity and the preservation of primary aromas reminiscent of grapes. Therefore, we hypothesised that applying phytohormones or mineral nutrients to leaves or carrying out defoliation can improve grape must properties in the face of climate warming and in accordance with current oenological trends. The effects of these three viticultural strategies were assessed independently from one another during three growing seasons in a Vitis vinifera L. cv. Tempranillo vineyard in northern Spain. Specifically, three 1-naphtaleneacetic acid (NAA) treatments, two early defoliations (ED; moderate and severe) and two foliar fertilisations with magnesium (Mg) were applied. Treatment with NAA was the most encouraging strategy for decreasing must TSS while increasing TA: it had slight effects on TSS in general and also slight effects on TA when applied close to veraison. The effects of the Mg treatments and moderate ED had null to slightly adverse effects. Finally, severe ED was clearly counter-productive. This study contributes to understanding the effects of both auxin and early defoliation treatments on grape must TSS, acidity and even yeast assimilable nitrogen (YAN) at harvest time. The favourable effects of NAA application are shown to be consistent though slight. Therefore, according to these results, the application of auxins may be an adequate choice for balancing sugars with acidity in grape musts. However, the results also suggest that more research needs to be undertaken to better characterise the effects of auxin treatments on grape must properties at harvest. In particular, different types of auxins, rates, concentrations and number of applications should be tested in the quest for more marked effects.

Influence of blistering lesions on foot functionality in hikers

BackgroundFriction blisters are formed by abrasion from frictional forces on the upper layer of the epidermis and can make physical activity an uncomfortable experience. To our knowledge, no previous studies have considered how these injuries affect the functionality of the foot. For this reason, the main aim of this study was to evaluate foot function in hikers, with or without blisters. Material and methodsThis case-control study examined 298 hikers who walked the Camino de Santiago long-distance trail (in northern Spain); 207 had one or more blistering foot lesions and 91 had no blisters. Sociodemographic and clinical variables were collected, and the number of blisters and their locations on the foot were recorded. All participants self-completed the Foot Function Index (FFI) questionnaire, in their native language. ResultsPain and disability were significantly greater among the hikers with blisters (pain p=<0.001; disability p = 0.015). However, there were no significant differences in the limitation of physical activity between those with blisters (case group) and the control group (p = 0.144). Neither was there any correlation between the number of blisters and pain, disability or limitation of activity. However, the location of the lesion did influence foot functionality. Blisters on the metatarsal heads were more limiting and caused greater pain (right foot p = 0.009; left foot p = 0.017), greater disability (right foot p = 0.005; left foot p = 0.005), greater limitation of activity (on right foot p = 0.012) and more loss of foot functionality (right foot p = 0.002; left foot p = 0.007). ConclusionThe hikers with blisters experienced reduced foot functionality in terms of pain and disability. The number of blisters was not related to foot functionality. Blisters located on the metatarsal heads caused the greatest increase in pain, disability and limitation of activity.