Normal Heat Research Articles

Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)

Heat stress, as a negative factor, severely threatens the quality and production of bottle gourd, which prefers to grow in a warm environment. To understand which genes are involved in the resistance to heat stress in bottle gourd (Lagenaria siceraria (Mol.) Standl.), we analyzed the characteristics of two genetic bottle gourd varieties, “Mei feng”-MF (heat resistant) and “Lv long”-LL (heat sensitive). Under heat stress, MF plants exhibited a higher survival rate, lower relative electrolytic leakage, and decreased stomatal aperture compared with LL. In addition, RNA-Seq was carried out on the two varieties under normal conditions and heat stress. The results revealed a total of 1485 up-regulated and 946 down-regulated genes under normal conditions, while 602 genes were up-regulated and 1212 genes were down-regulated under heat stress. Among these genes, several differentially expressed genes (DEGs) involved in the MAPK (mitogen-activated protein kinase) signaling pathway and members of bHLH (basic helix-loop-helix) transcription factors showed significant up- or down-regulation after heat stress. Next, to validate these findings, we conducted quantitative real-time PCR (qRT-PCR) analysis, which confirmed the expression patterns of the genes detected through RNA-Seq. Collectively, the DEGs between the two contrasting cultivars identified in our study provide novel insight into excavating helpful candidate genes associated with heat tolerance in bottle gourd.

Effects of Heat Stress on Breast Muscle Metabolomics and Lipid Metabolism Related Genes in Growing Broilers

With global warming and worsening climatic conditions, heat stress (HS) has become a significant challenge affecting the development of poultry production. In this study, we aimed to determine the effects of HS on breast muscle metabolomics and lipid metabolism-related genes in growing broilers. One hundred twenty 29-day-old Arbor Acres broilers were randomly divided into normal temperature (NT; 21 ± 1 °C) and heat stress (HS; 31 ± 1 °C) groups, with six replicates (ten birds in each replicate) in each group, raised for 14 days in two environment chambers at 60 ± 7% relative humidity. Compared with the broilers in the NT group, the average daily food intake, average daily gain and breast muscle yield in the HS group were significantly lower (p < 0.05). The feed conversion ratio was significantly higher in the HS group (p < 0.05). The concentrations of serum corticosterone, free fatty acids and cholesterol and the percentage of abdominal fat of broilers in the HS group were significantly higher (p < 0.05) than the values of the broilers in the NT group. Untargeted breast muscle metabolome analysis revealed 14 upregulated differential metabolites, including glycerophosphocholine, and 27 downregulated differential metabolites, including taurine, in the HS group compared to the NT group; the HS group also displayed significant effects on six metabolic pathways compared to the NT group (p < 0.05). The mRNA expression levels of peroxisome proliferator-activated receptor gamma coactivator-1-alpha, peroxisome proliferator-activated receptor alpha (PPARα) and ATP-binding cassette transporter A1 in the liver and breast muscles were significantly decreased in the HS group compared with the NT group (p < 0.05). The collective findings reveal that HS can cause disorders in breast muscle lipid metabolism in broilers. The PPARα gene might be the key gene in the mechanism of the lipid metabolism that is induced by HS in breast muscle of broilers. These findings provide novel insights into the effects of HS on chicken growth.

Promoting effects of alternating current and input power on grain growth behavior of cubic ZrO2 polycrystals

AbstractThe grain growth behavior during an AC flash event was examined in 8 mol% yttria‐stabilized cubic zirconia (8Y‐CSZ) polycrystals. The effects of current/power densities on the grain growth behavior were investigated in 8Y‐CSZ samples with different specific surface areas at a constant sample temperature and applied field strength. The grain growth rate of flash‐treated 8Y‐CSZ was 300 times faster than that of heat‐treated 8Y‐CSZ at the same sample temperature in the absence of an electric current/field, suggesting that the promoted grain growth cannot be ascribed only to a thermal effect but also to an athermal effect occurring during the AC flash event. Moreover, the grain growth during the flash treatment strongly depends on the applied current/power densities and grain size; in particular, the grain growth showed enhancements with increasing applied current/power densities and for relatively small grain sizes. This result suggests that the grain boundary diffusivity of cations, which are regarded as the rate‐controlling species for grain growth, could be accelerated by tuning the current/power densities during the flash event. The grain growth mechanism was characterized using a grain growth exponent (n) value of 4.8 for the flash treatment at high current/power densities and using a conventional value of n = 3 under normal heat treatment conditions. The dependence of the grain growth behavior on the AC current/power density suggests that because the cation diffusivity is accelerated due to the formation of numerous point defects during the AC flash event, the grain growth mechanism might depend on the current/power densities and differ from that of conventional grain growth.

Imposing different boundary conditions for thermal computational homogenization problems with FFT‐ and tensor‐train‐based Green's operator methods

AbstractTo compute the effective properties of random heterogeneous materials, a number of different boundary conditions are used to define the apparent properties on cells of finite size. Typically, depending on the specific boundary condition, different numerical methods are used. The article at hand provides a unified framework for Lippmann–Schwinger solvers in thermal conductivity and Dirichlet (prescribed temperature), Neumann (prescribed normal heat flux) as well as periodic boundary conditions. We focus on the explicit jump finite‐difference discretization and discuss different techniques for computing the discrete Green's operator. These include Fourier‐type methods, that is, based on discrete sine, cosine and Fourier transformations, as well as low‐rank tensor methods, that is, the tensor‐train approximation, whose computational prowess was demonstrated recently. We use the developed computational technology to investigate a number of interesting random materials and assess different microstructure‐generation techniques in terms of their compatibility with the prescribed boundary conditions.

Dissecting the resilience of barley genotypes under multiple adverse environmental conditions

As climate change increases abiotic stresses like drought and heat, evaluating barley performance under such conditions is critical for maintaining productivity. To assess how barley performs under normal conditions, drought, and heat stress, 29 different varieties were examined, considering agronomic, physiological, and disease-related characteristics. The research was conducted in five environments: two normal environments in 2020/2021 and 2021/2022, two drought stress environments in 2020/2021 and 2021/2022, and one heat stress environment in 2021/2022. The results demonstrated that genotype and environment significantly influenced all traits (p < 0.05), except canopy temperature, while genotype x environment interaction significantly influenced most traits, except total chlorophyll content and canopy temperature. Heat and drought stress environments often resulted in reduced performance for traits like plant height, spike length, grains per spike, and 100-grain weight compared to normal conditions. Based on individual traits, genotypes 07UT-44, 06WA-77, 08AB-09, and 07N6-57 exhibited the highest grain yield (4.1, 3.6, 3.6, and 3.6 t/ha, respectively). Also, these genotypes demonstrated enhanced stability in diverse drought and heat stress conditions, as assessed by the mean performance vs. stability index (Weighted Average of Absolute Scores, WAASB). The multi-trait stability index (MTSI) identified 07UT-44, 07UT-55, 07UT-71, and 08AB-09 as the most stable genotypes in terms of the performance of all traits. The imported lines demonstrated superior performance and stability, highlighting their potential as valuable genetic resources for developing climate-resilient barley.

Genetic variability, correlation and path analysis of yield and yield contributing characters in wheat (Triticum aestivum L.) under normal and terminal heat stress condition

Genetic variability and characters association studies play a pivotal role in enhancing selection efficiency under stress condition in plants. Therefore, the study was aimed at assessing the impact of terminal heat stress on various morpho-physiological traits and their association in wheat under control and terminal heat stress condition. The experiment was conducted using thirty-five wheat genotypes in a Randomized Complete Block design (RCBD) plots on three different sowing dates to induce terminal heat stress. A range of 11 morpho-physiological parameters were assessed. All of the traits showed significant variations under studied conditions. Estimates of the genotypic and phenotypic coefficients of variation (GCV and PCV) showed that PCV was higher than GCV indicating large environmental influence on the expression of the traits under study. High GCV and PCV were observed in grain per plant and yield per plant in both normal and heat stress conditions. Chlorophyll content, grain per plant and yield per plant showed highest heritability with high genetic advance (GA) under both normal and terminal heat stress condition. According to the results of the path co-efficient analysis, grain per plant had a strong positive connection with grain yield and plant height and days to 50% flowering had a negative correlation with grain yield per plant under both normal and stress condition. Canopy temperature has direct negative effect on yield in stress condition. The identified traits can be used as effective selection criteria under terminal heat stress condition to develop high yielding heat tolerant wheat variety.

Effect of flow direction on heat transfer and flow characteristics of supercritical carbon dioxide

This work is devoted to investigating the difference in flow and heat transfer characteristics between vertical upward flow and horizontal flow of supercritical carbon dioxide (&lt;inline-formula&gt;&lt;tex-math id="Z-20240119215215"&gt;\begin{document}$\rm sCO_2$\end{document}&lt;/tex-math&gt;&lt;alternatives&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215215.jpg"/&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215215.png"/&gt;&lt;/alternatives&gt;&lt;/inline-formula&gt;) based on the pseudo-boiling theory and the experimental parameters: mass flux &lt;i&gt;G&lt;/i&gt; = 496–1100 kg/m&lt;sup&gt;2&lt;/sup&gt;s, heat flux &lt;i&gt;q&lt;/i&gt;&lt;sub&gt;w&lt;/sub&gt; = 54.4–300.2 kW/m&lt;sup&gt;2,&lt;/sup&gt; and pressure &lt;i&gt;P&lt;/i&gt; = 7.531–20.513 MPa. The differences in flow and heat transfer characteristics between horizontal upward tube and vertical upward tube are compared at different mass fluxes, heat fluxes and pressures fully. Finally, unlike the classical treatment of flow and heat transfer for supercritical fluid, single-phase fluid assumption is abandoned, instead, the pseudo-boiling theory is introduced to deal with the flow transfer and heat transfer of &lt;inline-formula&gt;&lt;tex-math id="Z-20240119215113"&gt;\begin{document}$\rm sCO_2 $\end{document}&lt;/tex-math&gt;&lt;alternatives&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215113.jpg"/&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215113.png"/&gt;&lt;/alternatives&gt;&lt;/inline-formula&gt; in the two tubes. Supercritical fluid is regarded as a multiphase structure in this work, including a vapor-like layer near the wall and a liquid-like fluid in tube core. The results are indicated below. 1) In terms of heat transfer, the inner-wall temperature of the vertical upward tube and the bottom generatrix of horizontal tube are basically the same under normal heat transfer mode. When the heat transfer deterioration occurs in the vertical upward tube, larger supercritical boiling number (&lt;i&gt;SBO&lt;/i&gt;) will cause the wall temperature peak of the vertical upward tube to be much higher than the wall temperature at top generatrix of the horizontal tube at the corresponding enthalpy. The &lt;i&gt;SBO&lt;/i&gt; (&lt;i&gt;SBO&lt;/i&gt; = 5.126×10&lt;sup&gt;–4&lt;/sup&gt;) distinguishes between normal heat transfer deterioration and heat transfer deterioration in the vertical upward tube. In the horizontal tubes, &lt;i&gt;SBO&lt;/i&gt; dominates the maximum wall temperature difference between the top generatrix and the bottom generatrix. Comparing with vertical upward tubes, higher &lt;i&gt;q&lt;/i&gt;&lt;sub&gt;w&lt;/sub&gt;/&lt;i&gt;G&lt;/i&gt; is required for the heat transfer deterioration of supercritical fluid in the horizontal tubes under the same pressure. 2) In terms of flow, the increase in slope of pressure drop in the vertical upward tube is due to the orifice contraction effect. The mechanism that dominates the variation of pressure drop in the horizontal tube is the flow stratification effect, and we show that Froude number &lt;i&gt;Fr&lt;/i&gt;&lt;sub&gt;ave&lt;/sub&gt; can be the similarity criterion number to connect the temperature difference between the top and bottom generatrix of horizontal tube and the pressure drop. The analysis suggests that mechanisms governing horizontal flow and vertical flow of &lt;inline-formula&gt;&lt;tex-math id="Z-20240119215057"&gt;\begin{document}$\rm sCO_2 $\end{document}&lt;/tex-math&gt;&lt;alternatives&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215057.jpg"/&gt;&lt;graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="2-20231142_Z-20240119215057.png"/&gt;&lt;/alternatives&gt;&lt;/inline-formula&gt; are different in heat transfer deterioration mode. For the vertical flow, the &lt;i&gt;SBO&lt;/i&gt; plays a leading role, while for the horizontal flow, the &lt;i&gt;Fr&lt;/i&gt; plays an indispensable role.

Neutron irradiation effects on the low cycle thermal fatigue performance of first wall mock-ups

For the first wall (FW) of ITER, beryllium will be the plasma facing material and has to sustain high thermal, particle and neutron loads. In order to assess the synergistic effects of thermal and neutron loads in total ten Be-armored FW normal heat flux (NHF) mock-ups were produced consisting of two Be flat tiles each joined via hot isostatic pressing (HIP) to a CuCrZr heat sink and a steel support structure by different manufacturing routes to assess the most promising ones. Five of them were neutron irradiated, two at Centrum Výzkumu Řež in Czech Republic and three in the High Flux Reactor at NRG in the Netherlands. The remaining mock-ups were kept as reference. All flat-tile mock-ups had two Be-tiles and were exposed to cyclic steady state heat loads in the electron beam facilities JUDITH 1 and 2 up to a maximum power density of 3.75 MW/m2, in order to find the damaging threshold. A screening step using 1 MW/m2 was performed after finishing each loading step and after failure for direct comparison and detection of any deterioration caused by the cycling. Despite being still in the early development phase of Be joining, all mock-ups sustained the cycling up to at least 2.75 MW/m2 and clear differences in the performance of irradiated vs. non-irradiated mock-ups were observed.

Failure analysis for outer ring cracking of GCr15 rolling bearing

The purpose of this study analysis is to explore the reasons for the outer ring cracking of GCr15 rolling bearing. The failure mechanism was studied through observation of its microstructure and fracture morphology. The analysis results reveal that the main failure reason for the bearing was that the surface secondary quenching layers and network carbides caused the formation of cracks. In order to prevent failure, reasonable grinding process parameters, forging process parameters and normalizing heat treatment before spherodizing annealing were suggested.

Comparison of the Fleece Production of two Extensive French Sheep Genotypes

Sheep fleece became problematic Europe-wide for many reasons, including its very low price. From an animal welfare standpoint, the thick fleece may cause discomfort for the sheep as it can be a welcoming environment for parasites and may cause heat stress during hot summer days; therefore, annual shearing is necessary for most sheep genotypes. Physiologically, growing fleece and maintaining normal body temperature to decrease heat stress distract resources from producing valuable products such as meat. Thus, we aimed to compare the fleece production of two meat-type sheep genotypes from South-France, Berrichon du Cher (Berrichon) and Blanc du Massif Central (BMC), which have relatively little fleece production but thrive in the local Hungarian weather. Furthermore, we fill the gap by providing data on the fleece production of meat-type sheep in Hungary. A total of 180 sheep ewes were included in the data collection (N=99 Berrichon and N=81 BMC). Data was collected at the annual shearing by a digital scale measuring the weight of fleece originating from the back and shoulders. Student’s t test determined the differences in fleece weight between genotypes. Significance was set at P&lt;0.05. The average fleece production of BMC ewes was 1.66 kg, which was significantly (P=0.0001) less than that of Berrichon ewes (2.14 kg). Based on the results, further study is needed to study the performance of BMC ewes with little fleece and their lambs’ in order to improve performance indices and increase profit.

Effectiveness of novel artificial seabird nest modules for reducing ambient temperature transfer in a warming climate

AbstractArtificial habitat for cavity nesting birds can provide excellent opportunities for research and conservation efforts but may expose species to the negative impacts of warming ambient temperatures with climate change. Artificial nest boxes have been successfully used to monitor the breeding activity of the Cassin's auklet (Ptychoramphus aleuticus), a small burrow‐nesting seabird, on Southeast Farallon Island (SEFI) since 1971. Mean monthly ambient air temperatures on SEFI during the summer months have increased at an annual rate of roughly 0.03°C from 1971 to 2022, along with an increase in the number of extreme heat days and average maximum temperature, confirming a warming trend at this seabird colony. Given a projected increase in global temperature, we assessed the effectiveness of traditional wooden nest boxes vs. newer ceramic modules at buffering external ambient air temperatures in normal and extreme heat days across a gradient of microclimates on SEFI. Results from fitting linear mixed effects models indicated that, on average, internal temperatures of wooden and ceramic nests (of comparable size and shape) exhibited similar rates of deviation from ambient air temperature of approximately 0.15°C, even during extreme heat events. Ceramic modules did keep nest chambers cooler by approximately 1.2°C than wooden boxes during extreme events at the warmer, drier southern location of the island. Our results can help guide future efforts to design artificial nests that can effectively provide habitat for seabirds as ambient temperatures increase.

Prediction of thermal conduction in a four-phase composite with lowly-conducting interfaces based on variational asymptotic method

Due to the complex physical and chemical reactions, surface contamination and debonding, the interfaces within the four-phase composite, represented by hollow-glass-bead reinforced cement based material (HGB-CBM), cannot be assumed to be perfect. In order to consider the effect of imperfect interfaces, a novel thermal conduct model is developed. An imperfect interface is used to replace the pre-existing transition layer in the composite material, and the discontinuous temperature field proportional to the normal heat flow is preserved by using Hadamard’s relation and Taylor’s expansion. Based on the principle of minimum functional and variational asymptotic method, the governing variational statement for thermal conduct model is derived, and validated through comparison with RVE-based FEM and experimental results. It can be concluded that HGB-CBM with lowly-conducting imperfect interfaces has lower effective thermal conductivity, smaller heat flux within HGB and more significant changes at imperfect interfaces, which may be due to longer and more complex heat flux paths along the imperfect interfaces. HGB-CBM with larger volume fraction of HGB and smaller thermal conductivity of bead wall can provide better thermal insulation effect, which could provide reference for using HGB-CBM as thermal-insulating material in building engineering.

The Influence of the Deposition Speed during Friction Screw Extrusion Additive Manufacturing of AA6060

Solid-state additive manufacturing may solve critical issues typically arising during fusion-based additive manufacturing of high-strength aluminium alloys. In this work, the recently introduced Friction Screw Extrusion Additive Manufacturing (FSEAM) process was employed to manufacture wall-like rectangular builds of AA6060 T6 deposited with deposition speeds from 300 mm/min to 500 mm/min. All builds were manufactured at a tool rotation rate of 400 rpm with 1 mm layer thickness. The volumetric supply rates were adjusted to maintain constant build width. Solid builds were formed without major defects over the full range of deposition speeds. The process generated sufficient normal force and heat at all deposition speeds which resulted in manufacturing of defect free builds. The resulting average grain size was consistently below 5 micrometer throughout all builds independent of deposition speed or location through the height. Microhardness measurements revealed a decrease in hardness from a feedstock value of 80 HV to around 50 HV in all manufactured builds. Tensile tests in the building direction showed consistent results for all the samples as a result of defect-free parts, demonstrating a tensile strength of approximately 150 MPa, yield strength of 100 MPa, and uniform elongation of 12-15%. The fracture surfaces revealed large amounts of dimples at all deposition speeds in line with the high degree of plastic deformation preceding fracture observed from the tensile tests. The obtained results indicated that FSEAM is a promising process for solid-state additive manufacturing of aluminium alloys.

Synthesis of New Structural Analogues of Natural Integrastatins with a Basic Epoxybenzo[7,8]oxocine Skeleton: Combined Experimental and Computational Study

AbstractIn this work, the cyclization reactivity of various 3-acetyl-2-methylpyridines (including 3-acetyl-2-methylquinoline) containing both electron donor and acceptor substituents with salicylaldehyde into epoxybenzooxocino[4,3-b]pyridine derivatives was studied. The reactions were carried out in mild (under room temperature or reflux in 2-propanol) and harsh (in a sealed glass ampoule) conditions. It was shown that 3-acetyl-2-methylpyridines with an aryl substituent in the 4-position do not react with salicylaldehyde either under normal convection heating conditions or under more severe conditions. This effect was explained by the steric hindrance of the substituents using quantum chemical calculations. It was found that electron donor substituents in 3-acetyl-2-methylpyridines significantly facilitate cyclization in epoxybenzooxocino[4,3-b]pyridines. The presence of electron acceptor substituents (NO2 group for example) in the 5-position of pyridine prevents cyclization under normal conditions, but gives a rather high conversion to oxocinopyridines under more specific conditions. This effect is quantum-chemically explained by the decrease in the basicity of pyridine. Pyridines with two pairs of methyl groups in ortho-positions to the acetyl group are capable to form mixtures of regioisomeric epoxybenzooxocinopyridines. Further, epoxybenzooxocinopyridines with methyl and acetyl groups can form a mixture of diastereomeric bisoxocins under more specific conditions. All 17 initial pyridines were studied quantum-chemically in order to understand what features of their structure and properties affect the success of the cyclization reaction and the yield of the target product. The pyridine molecules were calculated by the DFT RB3LYP/6-311++G(d,p) method taking into account the alcohol solvent within the CPCM model using Gaussian-2016 program. It was shown that the absence of steric hindrances in the form of bulky substituents in 4-position of pyridines is the main factor affecting the success of the cyclization reaction. Also, the yield of the target product is affected by the CH-acidity of the methyl group in 2-position, which, in turn, is affected by electron-donating and electron-withdrawing substituents in the 5- and 6-positions.

A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes.

Cellular condensates are usually ribonucleoprotein assemblies with liquid- or solid-like properties. Because these subcellular structures lack a delineating membrane, determining their compositions is difficult. Here we describe a proximity-biotinylation approach for capturing the RNAs of the condensates known as processing bodies (PBs) in Arabidopsis (Arabidopsis thaliana). By combining this approach with RNA detection, in silico, and high-resolution imaging approaches, we studied PBs under normal conditions and heat stress. PBs showed a much more dynamic RNA composition than the total transcriptome. RNAs involved in cell wall development and regeneration, plant hormonal signaling, secondary metabolism/defense, and RNA metabolism were enriched in PBs. RNA-binding proteins and the liquidity of PBs modulated RNA recruitment, while RNAs were frequently recruited together with their encoded proteins. In PBs, RNAs follow distinct fates: in small liquid-like PBs, RNAs get degraded while in more solid-like larger ones, they are stored. PB properties can be regulated by the actin-polymerizing SCAR (suppressor of the cyclic AMP)-WAVE (WASP family verprolin homologous) complex. SCAR/WAVE modulates the shuttling of RNAs between PBs and the translational machinery, thereby adjusting ethylene signaling. In summary, we provide an approach to identify RNAs in condensates that allowed us to reveal a mechanism for regulating RNA fate.

Numerical analysis of the heat transfer behaviour of a wickless heat pipe receiver for parabolic trough solar collectors

The thermal performance of a wickless heat pipe receiver for parabolic trough solar collector applications is investigated in this study. For three filling ratios of 30%, 50%, and 90%, nine different heat flux profiles were examined. The normal heat flux range was chosen to be between 58 and 288 W/m2. The influence of the non-homogeneity of the thermal flux on the receiver tube was numerically analysed for various filling ratios. The maximum average wall temperature of 390 K was observed for heat transfer profile 7 and a fill ratio of 30%. For a filling ratio of 90%, the heat flux profile 7 had a minimum thermal resistance of 0.245 K/W, and a correlation was proposed to calculate the thermal resistance. A maximum thermal resistance of 1.722 K/W was calculated for the heat flux profile 3 and a filling ratio of 30%.

A comparative life cycle assessment on recycled concrete aggregates modified by accelerated carbonation treatment and traditional methods

Recycling of construction and demolition wastes contributes to achieve carbon summit and carbon neutrality early in the construction industry. Accelerated carbonation is a promising new technology for enhancing the properties of recycled concrete aggregates (RCAs) as well as mitigating global warming. This study performed a comparative life cycle assessment on RCAs modified by accelerated carbonation treatment and traditional methods. The effect of different treatment methods on environmental impacts of concrete was evaluated. The key contributors of environmental impacts for concrete incorporating carbonated RCAs were identified. Moreover, a sensitivity analysis on the transport distance of concrete incorporating carbonated RCAs was conducted. Results demonstrated that incorporating carbonated RCAs could significantly reduce the energy demand, environmental impacts and environmental cost compared with natural aggregate concrete. Accelerated carbonation treatment exhibited greater potential than the normal two-stage crushing and heating treatment in mitigating environmental burden, especially for the global warming potential. Cement production and transportation were the primary contributors to environmental impacts of concrete incorporating carbonated RCAs. Sensitivity analysis indicated incorporating carbonated RCAs as alternatives of natural aggregates contributes to lower the environmental impacts of concrete when the natural aggregates are far from urban areas while the recycling center is near the city.

Tensile behaviour of 42CrMo4 steel submitted to annealed, normalized, and quench and tempering heat treatments with in-situ hydrogen charging

42CrMo4 steel was submitted to annealing, normalizing, and quench and tempering heat treatments to produce steel microstructures with different hardness levels. Afterwards, tensile tests using notched specimens were conducted in air and in two different hydrogenated conditions by means of in-situ electrochemical hydrogen charging tests. Two different electrolytes and density currents were employed to produce low and high hydrogenated conditions. The influence of microstructure and hardness on hydrogen embrittlement was determined and scanning electron microscopy (SEM) analysis was used to identify the respective operative failure micromechanisms. A significant loss in notched strength was observed when hydrogen was introduced during the tensile tests on all tested microstructures, giving rise to changes in the predominant operative failure micromechanism. Embrittlement indexes increase as the electrochemical conditions applied introduce higher hydrogen concentrations and as the steel hardness increases. The notched tensile strength measured with in-situ hydrogen charging in the normalized and annealed grades is lower than that of quenched and tempered steels for the same hardness.

SAMSKARAH KARANAM MATAM WSR TO RASAPARPATI

Rasa Parpati is the type of Sagandha Parpati Kalpana. To prepare Parpati Kalpana, at least one ingredient should get liquefied at normal Heating and should get solidified when it gets cooled down, not only that, to get the thin flake Like structure specific pressure needs to be applied. In the present study Rasa Parpati was Prepared by using Samaguna Kajjali (equal quantity of Parada and Gandhaka) and to check the Cooling effect and the role of base platform; here three different platforms were made i.e., cow Dung and wet clay mixed platform, only wet clay platform (Earthen platform) and ice block Platform. Total nine samples of Rasa Parpati were prepared, three on each platform to check Which platform is convenient to prepare Rasa Parpati. The average thickness of Rasa Parpati Was found to be minimum (2.83mm) on cow dung and wet clay mixed platform whereas the Maximum (4mm) on ice block platform i.e., 4mm. Average thickness of Rasa Parpati made on Earthen platform was 3.57mm which is thicker than the Cow dung and wet clay mixed Platform and thinner than the ice cube platform. Which indicates the best platform to make Thinnest Parpati is cow dung and wet clay mixed platform.

Only one of three hydrophobins (Hyd1–3) contributes to conidial hydrophobicity and insect pathogenicity of Metarhizium robertsii

Class I/II hydrophobins constitute a family of small amphiphilic proteins that mediate cell hydrophobicity and adhesion to host or substrata and have pleiotropic effects in filamentous fungi. Here we report that only class I Hyd1 is essential for conidial hydrophobicity and insect pathogenicity among three hydrophobins (Hyd1–3) characterized in Metarhizium robertsii, an insect-pathogenic fungus. Aerial conidiation levels of three Δhyd1 mutants were much more reduced in 5-day-old cultures than in 7-day-old cultures, which were wettable (hydrophilic), but restored to a wild-type level in 15-day-old cultures. The Δhyd1 mutants were compromised in conidial quality, including significant decreases in hydrophobicity (58%), adhesion to insect cuticle (36%), insect pathogenicity via normal cuticle infection (37%), UVB resistance (20%), and heat tolerance (10%). In contrast, none of all examined phenotypes were affected in the null mutants of hyd2 and hyd3. Intriguingly, micromorphology and integrity of hydrophobin rodlet bundles on conidial coat were not affected in all mutant and wild-type strains, but the rodlet bundles were disordered in the absence of hyd1, suggesting a link of the disorder to the decreased hydrophobicity. Therefore, Hyd1 mediates the fungal hydrophobicity and plays an important role in conidial quality control and insect-pathogenic lifecycle. Class I Hyd2 and class II Hyd3 seem functionally redundant in M. robertsii.