Normal Conditions Research Articles

Estimate of genetic components for yield and its component under normal and heat stress conditions in pea (Pisum sativum L.)

Estimate of genetic components for yield and its component under normal and heat stress conditions in pea (Pisum sativum L.)

Inheritance and Stability of Grain Yield Traits and Its Components under Salt and Normal Conditions of Bread Wheat Genotypes (Triticum aestivum L.)

Inheritance and Stability of Grain Yield Traits and Its Components under Salt and Normal Conditions of Bread Wheat Genotypes (Triticum aestivum L.)

Peristaltic Flow of Jeffrey Fluid in an Inclined Porous Tube with Multi-Thrombosis

The fact that multiple thrombosis opposes blood flow and flow is rare in blood vessels is refined to a normal condition with catheter application. The inner surface of the circular blood arteries may not be smooth in the majority of diseased instances. Additionally, the small blood vessel peristalsis mechanism is formed. In this paper, we discuss a mathematical model for the flow of biological fluid (blood) in a circular tube with multi-thrombosis considered under peristaltic wave propagation. The blood flow in this tube is restricted due to the many thromboses present, and the flow is redesigned with the aid of a catheter. We model this non-Newtonian blood flow issue for Jeffrey fluid. In most pathological cases, the inner surface of the circular blood vessels may not be smooth. Further, the mechanism of the peristalsis is established for small blood vessels. Because of this biological phenomenon, the peristaltic movement of Jeffrey fluid in a porous annulus with slip is examined. Equations that govern energy and momentum are solved exactly, and graphical interpretation is made using mathematical software. Streamline graphs show the many thromboses that increase in height. The wall shear stress graphs show a sinusoidally advancing wave with peaks and dips of different amplitudes. This tube's unique crest and trough amplitude are caused by the presence of multiple thromboses. We obtained the impact of the pressure gradient on the permeability parameter (β). The pressure gradient falls as the permeability parameter rises.

End-to-end deep learning on classification of neutral-to-ground voltage in secondary distribution systems

Neutral-to-ground voltage (NTGV) abnormalities in secondary distribution systems (SDS) pose significant power quality (PQ) challenges, including safety hazards, power losses, and equipment damage. Despite their importance, these abnormalities remain relatively understudied. Accurate classification of NTGV events is crucial for effective mitigation strategies. Existing research primarily relies on machine learning (ML) models trained on manually extracted features from simulated or real-world signals. This paper introduces a novel end-to-end deep learning approach that leverages Gate Recurrent Units (GRU) to bypass manual feature extraction, directly utilizing real-world signals from three NTGV event categories: ground fault, lightning strike, and normal conditions. This is first time that GRU has been used for NTGV classification using raw data. The model's generalizability is assessed through 5-fold cross-validation. A comparative analysis with baseline models and traditional ML techniques demonstrates the proposed model's superior performance and computational efficiency due to its ability to directly process raw data.

Correlation between social intelligence and motor development of children in the 4.0 era

Social intelligence and motor skills are essential for a child's development. This study in the Sleman Regency investigates the relationship between social intelligence and motor skills development in 170 primary school students. The data collection instruments included a Social Skills Rating Scale (SSRS-T), a questionnaire, and Nurhasan’s motor ability test. The data used meet the normality and linearity conditions, as indicated by the coefficients of 0.721 and 0.108, each greater than 0.05, respectively. The results show that 71.17% of the sampled students have above-average social intelligence (SI). The sampled children’s SI can be grouped into very good (top 10%), good (next 18.82%), average (middle 42.35%), poor (next 25.29%), and extremely poor (bottom 25.29%); the respondents' rates in each category are as mentioned. Regarding motor development, 8.24% of the students were categorised as very good, 20.59% as good, 36.47% as average, 32.35% as poor, and 2.35% as extremely poor. The study found a moderate positive correlation (r value of 0.344) between social intelligence and motor development. With the determinant R2=0.118, given the variable indicators, high importance on communication and physical interaction explains at least 11.8% of the variation in the students’ motor skills development. The researchers recommend that further studies on the habits of electronic gadget usage by the sampled children be carried out to shed light on unexploited frontiers for children with poor and extremely poor social intelligence.

Impact of the Shape of the Skull Base on Morphometric Parameters of the First and Second Cervical Vertebrae in 8–12-Year-Old Children: Assessment of the Condition Under Norm and Recurrent Subluxation in the C1–C2 System

The aim of the study is to determine the impact of the shape of the skull base on the morphometric parameters of the first (C1) and second (C2) cervical vertebrae in children aged 8–12 under normal conditions and under recurrent subluxation of C1–C2. Materials and methods. The authors analysed computed tomography (CT) scans of 212 children aged 8–12 without pathology of the cervical spine and 65 patients of the same age group with recurrent subluxations of the C1-C2 vertebrae. Computed tomography was performed on a computer tomography GE OPTIMA CT 660, 64-slice machine (Russia). CT morphometry was carried out using the Radiant program (Russia). The width and length of the skull base, parameters of the first (C1) and second (C2) cervical vertebrae, and interosseous parameters were determined, the basilar index was calculated; three groups of individuals with dolicho-, meso-, or brachybasilar shape of the skull base were formed based on the value of the basilar index. One-way analysis of variance was carried out. Results The impact of the shape of the skull base of moderate strength on the transverse dimensions of the upper articular surfaces of C1 on the right and left in children without pathology of the cervical spine was revealed. Among the examined individuals with recurrent subluxation in the C1–C2 system, there was a strong connection between the shape of the skull base and the longitudinal dimensions of the upper articular surfaces of the atlas. A moderately close relationship was revealed between the shape of the skull base and the height of the body of the second cervical vertebra and with the angle of inclination of the C2 tooth in the sagittal plane in those examined without pathology of the cervical spine. In children with recurrent subluxation, a strong relationship of the controlled factor was determined with the height of the C2 body and the angle of inclination of its tooth in the sagittal plane, and a mediumstrong relationship with the sagittal diameter of the tooth. The influence of the shape of the skull base of moderate strength on the size of the anterior atlantoaxial joint space was revealed both in children without pathology of the cervical spine and in children with recurrent subluxation. In case of right-sided subluxation, a mediumstrong relationship between the shape of the skull base and the lateral distance between the base of the tooth and the medial surface of the lateral mass of C1 on the side contralateral to the subluxation was revealed. Conclusion. The shape of the skull base affects a number of anatomical structures of the first two cervical vertebrae in children aged 8–12.

Sustainable poultry practices: integrating green light interventions to control pecking in chicken

BackgroundThe present study aimed to investigate the impact of the light-emitting diode (LED) green light alone or in combination with melatonin on pecking-related hormone regulation during incubation under normal and under hormonal stress conditions in breeder eggs. This study was divided into 2 experiments: In the first experiment effect of LED green light incubation on pecking-related hormones under normal conditions, on Hy-line brown (low pecking phenotype) and Roman pink (high pecking phenotype) eggs were tested. The 296 eggs of each strain were divided into two groups: LED green light incubation and dark incubation (control), each containing four replicates (37 eggs/replicate). The second experiment was conducted to evaluate the effect of LED green light incubation alone or in combination with melatonin under hormonal stress conditions on Roman pink eggs. A total of 704 Roman pink eggs were taken and divided into four groups, each consisting of 176 eggs. Each group was further divided into 2 subgroups, LED green light-regulated incubation and dark incubation with 88 eggs per subgroup, having 4 replicates of 22 eggs each. The groups were as follows: corticosterone solution injection (CI), corticosterone + melatonin mixed solution injection (CMI), Phosphate buffer solution injection (PI), and no injection (UI).ResultsResults of the first experiment revealed a higher level of serotonin hormone and lower corticosterone hormone in Hy-Line brown embryos compared to Roman pink embryos during dark incubation. The LED green light incubation significantly (P < 0.05) increased the level of 5-HT while decreasing the CORT level in Roman pink embryos indicating its regulatory effect on pecking-related hormones. Results of the second experiment showed that LED green light incubation significantly (P < 0.05) alleviated the CORT-induced hyperactivity of plasma 5-HT in Roman pink embryos. Furthermore, Melatonin (MLT) injection and LED green light together significantly (P < 0.05) reduced the hormonal stress caused by corticosterone injection in the eggs.ConclusionsOverall, the LED green light regulatory incubation demonstrated a regulatory effect on hormones that influence pecking habits. Additionally, when coupled with MLT injection, it synergistically mitigated hormonal stress in the embryos. So, LED green light incubation emerged as a novel method to reduce the damaging pecking habits of poultry birds.

Transcription Factor NF-κB: Role and Significance in the Neuroimmunoendocrine Regulation of Respiratory Function in Normal Conditions and in Lung Pathology

Transcription Factor NF-κB: Role and Significance in the Neuroimmunoendocrine Regulation of Respiratory Function in Normal Conditions and in Lung Pathology

Gene pyramiding improved cell membrane stability under heat stress in cotton (Gossypium hirsutum L.)

Climate change has been drastically affecting cotton not only in Pakistan but also all over the world. Normally cotton is known as heat tolerant when compared with other crops, but if the high temperature occurs during flowering period the yield decreases significantly. Marker assisted gene pyramiding provides a sustainable solution to improve heat tolerance. A total of seven genotypes were developed by a series of crossing seven tolerant genotypes over the period of three years. Tolerant genotypes were selected by screening for important transcription factors (GHSP26, HSP3, HSFA2, DREB1A, HSP101, DREB2A, GhNAC2, HSPCB, GhWRKY41, TPS, GbMYB5, ANNAT8, GhMPK17, GhMKK1, GhMKK3, GhMPK2, HSC70, APX1 and GhPP2A1). The seven genotypes were evaluated under normal and heat stress in a multi-year trial. The traits related to heat tolerance, such as cell membrane stability, relative water content, excised leaf water loss, plant height, number of nodes, internodal length, number of buds, number of bolls and leaf area was observed under normal and heat stress conditions. The developed genotypes showed improvement in cell membrane stability and relative water content under heat stress. The genotypes [(VH-305×MNH-886)×MNH-1035)×NIAB-78)], [(MNH-1035×MNH-886)×MNH-886)×SM-431] and [(MNH-1035×MNH-886)×MNH-886)×SS-32] depicted heat tolerance and could be used as heat tolerant material for variety development in breeding programs.

Forward–reverse mutation cycles in cancer cell lines under chemical treatments

Spontaneous forward–reverse mutations were reported by us earlier in clinical samples from various types of cancers and in HeLa cells under normal culture conditions. To investigate the effects of chemical stimulations on such mutation cycles, the present study examined single nucleotide variations (SNVs) and copy number variations (CNVs) in HeLa and A549 cells exposed to wogonin-containing or acidic medium. In wogonin, both cell lines showed a mutation cycle during days 16–18. In acidic medium, both cell lines displayed multiple mutation cycles of different magnitudes. Genomic feature colocalization analysis suggests that CNVs tend to occur in expanded and unstable regions, and near promoters, histones, and non-coding transcription sites. Moreover, phenotypic variations in cell morphology occurred during the forward–reverse mutation cycles under both types of chemical treatments. In conclusion, chemical stresses imposed by wogonin or acidity promoted cyclic forward–reverse mutations in both HeLa and A549 cells to different extents.

Expansion and diversification of the Glycine max (Gm) ERD15-like subfamily of the PAM2-like superfamily.

Despite modulating senescence and drought responses, the GmERD15-like subfamily members are differentially induced by multiple stresses and diverge partially in stress signaling functions. The PAM2 motif represents a binding site for poly (A)-binding proteins (PABPs), often associated with RNA metabolism regulation. The PAM2-containing protein ERD15 stands out as a critical regulator of diverse stress responses in plants. Despite the relevance of the PAM2 motif, a comprehensive analysis of the PAM2 superfamily and ERD15-like subfamily in the plant kingdom is lacking. Here, we provide an extensive in silico analysis of the PAM2 superfamily and the ERD15-like subfamily in soybean, using Arabidopsis and rice sequences as prototypes. The Glycine max ERD15-like subfamily members were clustered in pairs, likely originating from DNA-based gene duplication, as the paralogs display high sequence conservation, similar exon/intron genome organization, and are undergoing purifying selection. Complementation analyses of an aterd15 mutant demonstrated that the plant ERD15-like subfamily members are functionally redundant in response to drought, osmotic stress, and dark-induced senescence. Nevertheless, the soybean members displayed differential expression profiles, biochemical activity, and subcellular localization, consistent with functional diversification. The expression profiles of Glyma04G138600 under salicylic acid (SA) and abscisic acid (ABA) treatments differed oppositely from those of the other GmERD15-like genes. Abiotic stress-induced coexpression analysis with soybean PABPs showed that Glyma04G138600 was clustered separately from other GmERD15s. In contrast to the AtERD15 stress-induced nuclear redistribution, Glyma04G138600 and Glyma02G260800 localized to the cytoplasm, while Glyma03G131900 fractionated between the cytoplasm and nucleus under normal and stress conditions. These data collectively indicate that despite modulating senescence and drought responses, the GmERD15-like subfamily members are differentially induced by multiple stresses and may diverge partially in stress signaling functions.

Blood-Brain Barrier Conquest in Glioblastoma Nanomedicine: Strategies, Clinical Advances, and Emerging Challenges.

Glioblastoma (GBM) is a prevalent type of malignancy within the central nervous system (CNS) that is associated with a poor prognosis. The standard treatment for GBM includes the surgical resection of the tumor, followed by radiotherapy and chemotherapy; yet, despite these interventions, overall treatment outcomes remain suboptimal. The blood-brain barrier (BBB), which plays a crucial role in maintaining the stability of brain tissue under normal physiological conditions of the CNS, also poses a significant obstacle to the effective delivery of therapeutic agents to GBMs. Recent preclinical studies have demonstrated that nanomedicine delivery systems (NDDSs) offer promising results, demonstrating both effective GBM targeting and safety, thereby presenting a potential solution for targeted drug delivery. In this review, we first explore the various strategies employed in preclinical studies to overcome the BBB for drug delivery. Subsequently, the results of the clinical translation of NDDSs are summarized, highlighting the progress made. Finally, we discuss potential strategies for advancing the development of NDDSs and accelerating their translational research through well-designed clinical trials in GBM therapy.

Preparation of Chiral Stationary Phase Based on 1,1'-bi-2-Naphthol for Chiral Enantiomer Separation.

Two enantiomeric novel chiral stationary phases (CSPs) R-3-Amide-BINOL CSP (CSP-1) and S-3-Amide-BINOL CSP (CSP-2) were prepared using (R/S)-1,1'-bi-2-naphthol (BINOL) derivatives as chiral selectors. The structure of CSPs was characterized by nuclear magnetic resonance, scanning electron microscope and elemental analysis. Four chiral solutes were selected under normal phase HPLC conditions to evaluate the chiral separation ability of the two novel CSPs. The effects of mobile phase and acidic additives on enantiomeric separation were investigated. The combination of molecular docking simulation and experimental data has elucidated the crucial role of hydrogen bonds and π-π interactions formed between the analyte and CSP in chiral recognition, and different configurations of CSP can cause enantiomeric elution sequence reversal, indicating that the configuration of chiral selectors in CSP has a significant impact on chiral recognition ability.

Strigolactone enhances tea plant adaptation to drought and Phyllosticta theicola petch by regulating caffeine content via CsbHLH80

Strigolactones (SLs) play crucial roles in both plant growth and stress responses. However, their impact on the secondary metabolites of woody plants remains elusive. Here, we found that exogenous strigolactone analogue GR24 positively regulates tea plant flavor secondary metabolites, concurrently inhibiting caffeine biosynthesis and promoting the accumulation of caffeine catabolic pathway products. In this process, SL directly or indirectly inhibits the expression of CsSAMSs by inducing CsbHLH80, thereby reducing caffeine biosynthesis. Furthermore, CsbHLH80 enhances caffeine degradation, leading to increased allantoin. Under normal conditions, heightened allantoin reduces abscisic acid (ABA) accumulation. This inhibition reverses under drought stress. Increased ABA significantly enhances tea plant tolerance to both drought and Phyllosticta theicola Petch. In summary, this study offers novel insights for improving tea plant adaptation and quality in arid regions, particularly emphasizing the selection of stress-tolerant varieties and the refinement of production measures with a focus on high-quality production and environmentally friendly biological control methods.

Application of cascaded neural network for prediction of voltage stability margin in a solar and wind integrated power system

Voltage stability is a paramount concern in the management of renewable-rich power systems. As the diffusion of renewable energy sources continues to increase, accurately estimating voltage stability margins becomes essential. This paper addresses the critical need for effective voltage stability margin estimation and presents a novel approach to predict the same on the solar and wind integrated power system. A new machine learning methodology is developed based on the cascaded neural network (CNN). The proposed CNN methodology effectively estimates the loadability margin in a renewable-rich power system under normal operating conditions, varied loading directions, and various contingency situations. The results of CNN are compared with different machine learning techniques and tested in the IEEE 30 bus system and IEEE 118 bus system under various operational circumstances. The results illustrate the efficiency of the suggested method for online assessment of a power system's Voltage Stability Margin. The proposed approach gives real-time insights into voltage stability margins by leveraging the power of data and powerful algorithms, allowing grid operators to proactively manage and optimize renewable energy integration while ensuring grid dependability and stability.

Dysfunction of Endothelial Cell-Mediated Intercellular Communication and Metabolic Pathways in Age-Related Macular Degeneration

Purpose Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly, but the therapies are not satisfactory. This study aimed to find AMD specific features through the analysis of high-throughput sequencing. Methods In this study, we integrated six projects containing single-cell RNA sequencing (scRNA-seq) data to perform a comprehensive analysis for AMD samples in the tissues of retina and retinal pigment epithelium/choroid, and in the positions of macula and periphery. Differentially expressed genes (DEGs) were analyzed and crucial signaling pathways were identified across cell types and between the macula and periphery. The intercellular signaling transduction among cell types were inferred by "CellChat" to build cell–cell communication network under normal and AMD conditions, and verified at the transcriptional level. The CD31+ endothelial cells were obtained to evaluate the enrichment of KEGG pathways in atrophic and neovascular AMD, and GSVA was adopted to discover differential metabolic signals in each AMD type. Results Thirteen major cell types were identified in the integrated scRNA-seq data. Although no disease-specific cell type or differential cell proportion was found, DEGs and enriched pathways were shown in cell-type- and position-dependent manners. Severe impairment of endothelial cell-mediated cell interactions was found in the signaling transduction network of the macula, and compromised cell interactions were observed in the periphery. Furthermore, distinct signaling pathways and metabolic states were uncovered in atrophic and neovascular AMD. Striking reduction in energy metabolism, lipid metabolism, and oxidative stress was indicated in the atrophic AMD. Conclusion Conclusively, we discover aberrant signals and metabolic pathways in AMD samples, providing insight into mechanisms and potential therapeutic targets for the AMD treatment.

Quantitative Assessment of Polarization and Elastic Properties of Endometrial Tissue for Precancer/Cancer Diagnostics Using Multimodal Optical Coherence Tomography

Objectives: The most important phase in the endometrial pathologies diagnostics is the histological examination of tissue biopsies obtained under visual hysteroscopic control. However, the unclear visual diagnostics characteristics of subtle focal endometrial pathologies often lead to selection errors regarding suspicious endometrial lesions and to a subsequent false pathological diagnosis/underestimation of precancer or early-stage cancer. Methods: In this study, we investigate the potential of Multimodal Optical Coherence Tomography (MM OCT) to verify suspicious endometrial lesion regions before biopsy collection. We study the polarization (by cross-polarization OCT, CP OCT) and elastic (by compression OCT-elastography, C-OCE) properties of ex vivo endometrial tissue samples in normal conditions (proliferative and secretory phases to the menstrual cycle, atrophic endometrium) with endometrial hyperplasia (non-atypical and endometrial intraepithelial neoplasia) and endometrial cancer subtypes (low-grade, high-grade, clear cell and serous). Results: To the best of our knowledge, this is the first quantitative assessment of relevant OCT parameters (depth-resolved attenuation coefficient in co-[Att(co) values] and cross-[(Att(cross) values] polarizations and Young’s elastic modulus [stiffness values]) for the selection of the most objective criteria to identify the clinically significant endometrial pathologies: endometrial intraepithelial neoplasia and endometrial cancer. The study demonstrates the possibility of detecting endometrial pathologies and establishing optimal threshold values of MM OCT criteria for the identification of endometrial cancer using CP OCT (by Att(co) values = 3.69 mm−1, Sensitivity (Se) = 86.1%, Specificity (Sp) = 92.6%; by Att(cross) values = 2.27 mm−1, Se = 86.8%, Sp = 87.0%) and C-OCE (by stiffness values = 122 kPa, Se = 93.2%, Sp = 91.1%). The study also differentiates endometrial intraepithelial neoplasia from non-atypical endometrial hyperplasia and normal endometrium using C-OCE (by stiffness values = 95 kPa, Se = 87.2%, Sp = 90.1%). Conclusions: The results are indicative of the efficacy and potential of clinical implementation of in vivo hysteroscopic-like MM OCT in the diagnosis of endometrial pathologies.

EVALUATING CUT SLOPE STABILITY DURING RAINFALL EVENTS IN HA GIANG CITY, VIET NAM

The main objective of the present study is to investigate the mechanism of triggering slope failures during extreme rainfall events under the climatic and geological conditions of Ha Giang city, Viet Nam. For this purpose, the specific research objectives of this paper were based on the analytic results of the geotechnical properties of the soil samples. The pore water pressure distribution on a slope was changed by the influence of rainfall. In order to analyze the stability of the cut slopes, the two modules SEEP/W and SLOPE/W in the GEOSTUDIO 2012 software were employed and integrated. First, the initial steady seepage condition was used to assess the effect of extreme rainfall for a transient seepage. Second, the soil sample properties and some features were assigned in the slope stability model, such as unit weight, cohesion, internal friction angle, and hydraulic functions, and then the achieved transient pore water pressure regime was used as the input parameter to calculate the factor of safety (FS) on cut slopes. Finally, the relationship between the changing of the FS over time and the hourly rainfall intensity that resulted in slope failure were evaluated. In summary, the slopes in Ha Giang city can be stable under normal conditions, but their stability is always a dilemma for local authorities due to the influence of intense rainfall.

The structure elucidation, anti-aging and hypoglycemic effects of an O-acetyl mannoglucan from the bulbs of Lanzhou lily

An O-acetyl mannoglucan (BHP-1) from Lanzhou lily bulbs was structurally elucidated using partial acid hydrolysis, GC–MS, and 2D NMR techniques (COSY, NOESY, HSQC and HMBC) built on prior research, revealing a backbone of -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp- with the most potential side chains -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → 4)-Glcp-α-D-(1 → Glcp- and -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → Glcp-, attached to O-2 and O-3 of glucose and mannose residues, and featuring O-acetyl groups at O-2 or O-3 position of mannose. The terminal residue was α-D-(1 → Glcp. BHP-1 demonstrated anti-aging and hypoglycemic effects, as assessed by C. elegans model and glycolytic enzyme effect in vitro, respectively. The results showed that BHP-1 dose-dependently prolonged lifespan of C. elegans by 33 % at 4 mg/mL under normal conditions, with greater extensions under thermal and oxidative stress (50 % and 80 % increases, respectively, p < 0.05), which were attributed to enhanced antioxidant enzymes (SOD and CAT) and lowered MDA levels of C. elegans. Additionally, BHP-1 exhibited remarkable inhibition on α-glucosidase (93 %) and moderate inhibition on α-amylase (53 %) at 4 mg/mL, with competitive inhibition of α-glucosidase and mixed non-competitive inhibition of α-amylase, respectively. These potential effects might be linked to BHP-1's diverse sugar linkages, higher content of Glc, and certain O-acetyl contents.

GSTT1/GSTM1 deficiency aggravated cisplatin-induced acute kidney injury via ROS-triggered ferroptosis.

Cisplatin is a widely used chemotherapeutic agent prescribed to treat solid tumors. However, its clinical application is limited because of cisplatin- induced nephrotoxicity. A known complication of cisplatin is acute kidney injury (AKI). Deletion polymorphisms of GSTM1 and GSTT1, members of the glutathione S-transferase family, are common in humans and are presumed to be associated with various kidney diseases. However, the specific roles and mechanisms of GSTM1 and GSTT1 in cisplatin induced AKI remain unclear. To investigate the roles of GSTM1 and GSTT1 in cisplatin-induced AKI, we generated GSTM1 and GSTT1 knockout mice using CRISPR-Cas9 technology and assessed their kidney function under normal physiological conditions and cisplatin treatment. Using ELISA kits, we measured the levels of oxidative DNA and protein damage, along with MDA, SOD, GSH, and the GSH/GSSG ratio in wild-type and GSTM1/GSTT1 knockout mice following cisplatin treatment. Additionally, oxidative stress levels and the expression of ferroptosis-related proteins in kidney tissues were examined through Western blotting, qPCR, immunohistochemistry, and immunofluorescence techniques. Here, we found that GSTT1 and GSTM1 were downregulated in the renal tubular cells of AKI patients and cisplatin-treated mice. Compared with WT mice, Gstm1/Gstt1-DKO mice were phenotypically normal but developed more severe kidney dysfunction and exhibited increased ROS levels and severe ferroptosis after injecting cisplatin. Our study revealed that GSTM1 and GSTT1 can protect renal tubular cells against cisplatin-induced nephrotoxicity and ferroptosis, and genetic screening for GSTM1 and GSTT1 polymorphisms can help determine a standard cisplatin dose for cancer patients undergoing chemotherapy.