Strigolactone enhances tea plant adaptation to drought and Phyllosticta theicola petch by regulating caffeine content via CsbHLH80

Abstract

Strigolactones (SLs) play crucial roles in both plant growth and stress responses. However, their impact on the secondary metabolites of woody plants remains elusive. Here, we found that exogenous strigolactone analogue GR24 positively regulates tea plant flavor secondary metabolites, concurrently inhibiting caffeine biosynthesis and promoting the accumulation of caffeine catabolic pathway products. In this process, SL directly or indirectly inhibits the expression of CsSAMSs by inducing CsbHLH80, thereby reducing caffeine biosynthesis. Furthermore, CsbHLH80 enhances caffeine degradation, leading to increased allantoin. Under normal conditions, heightened allantoin reduces abscisic acid (ABA) accumulation. This inhibition reverses under drought stress. Increased ABA significantly enhances tea plant tolerance to both drought and Phyllosticta theicola Petch. In summary, this study offers novel insights for improving tea plant adaptation and quality in arid regions, particularly emphasizing the selection of stress-tolerant varieties and the refinement of production measures with a focus on high-quality production and environmentally friendly biological control methods.