Graphene's appeal in various applications stems from its unique properties like remarkable electrical conductivity, impressive mechanical strength, expansive surface area, minimal thermal expansion, and a high aspect ratio. However, its insolubility in water poses challenges for its use in the biomedical sector. This research focuses on developing a method to produce water-soluble and biocompatible graphene suitable for biomedical uses. The study successfully created a stable solution of graphene oxide (GO) in water using a non-ionic Pluronic copolymer. This GO-tripolymer solution maintained its solubility in phosphate buffered saline (PBS) and displayed similar UV absorption characteristics to standard GO. Given the toxicity concerns with traditional reducing agents like hydrazine, this study opted for safer alternatives. Non-toxic substances such as ascorbic acid, galactose, and bovine serum albumin were employed to reduce GO, resulting in a reduced GO (RGO) -tripolymer solution that also showed excellent solubility in PBS. The final phase of the study involved assessing the cytotoxicity of these RGO-tripolymers. The results indicated that these novel RGO-tripolymers exhibit low levels of cytotoxicity, making them potentially suitable for biomedical applications.Keywords: Graphene Oxide (GO), Biocompatibility, Non-Toxic Reducing Agents, Water Solubility and Biomedical Applications