Studied level of milk production and milk quality of Holstein breed depending on origin and linear conditioning animals. Found that the largest average daily milk production of cow enough adult Danish, German and Hungarian origin surpassed the requirements of the standard breed at 7.9; 8.8 and 6.9 kg or 32.5; 29.7 and 35.1%. Their daughter exceeded breed standard at 9.2; 8.8 and 11.1 kg or 36.1; 36.0 and 40.5%, respectively.On the content of fat in milk breed standard (3.6%) match only imported cows and their daughter of Danish and German breeding. However, the import of cows from the Danish, German and Hungarian origin protein content in milk was below the standard of the breed to 0.2; 0.4 and 0.2%; animals first generation on this indicator inferior requirement standard 0.1, respectively; 0.5 and 0.8%. The fat content in the milk turned out to be higher in imported cows and their daughters Starbuck line 352790.79 (3.6 and 3.7%). On the content of protein in milk cows imported lines have supremacy Bella 1667366.74 (3.1%). Moderately high content of this component in milk observed in cows patriotic line generation Eleveyshn 1491007.65 (3.2%). The most nutritious milk from cows imported turned out to line Cheef 1427381.62 (61.2 kcal/100 g). They also differed with significant content of nonfat milk solids (8.42%). Starbuck’s daughter 352790.79 surpassed their mothers on the content of the skimmed milk residue (8.45%) is water.High in lactose different imported cows lines Cheef 1427381.62 and Bella 1667366.74 (4.63%). in turn, the only daughter of the Starbuck 352790.79 line proved to be the best on the content of milk sugar.The data obtained is cut off the opportunity to conduct breeding work to improve desired traits.