Non-traditional Functions Research Articles

Active sampling as an information seeking strategy in primate vocal interactions

Active sensing is a behavioral strategy for exploring the environment. In this study, we show that contact vocal behaviors can be an active sensing mechanism that uses sampling to gain information about the social environment, in particular, the vocal behavior of others. With a focus on the real-time vocal interactions of marmoset monkeys, we contrast active sampling to a vocal accommodation framework in which vocalizations are adjusted simply to maximize responses. We conduct simulations of a vocal accommodation and an active sampling policy and compare them with actual vocal interaction data. Our findings support active sampling as the best model for real-time marmoset monkey vocal exchanges. In some cases, the active sampling model was even able to partially predict the distribution of vocal durations for individuals to approximate the optimal call duration. These results suggest a non-traditional function for primate vocal interactions in which they are used by animals to seek information about their social environments.

Вооруженные силы Латинской Америки: силовой потенциал, новые функции и фактор внерегиональных связей

Introduction. As a state institution, the Armed Forces of countries in Latin America maintain certain development dynamics in the 21st century. The analysis of those problems gives an opportunity to determine the armed force potential of the Latin American region. Methods and materials. The study is based on distinguishing between the quantitative and qualitative aspects of the transformation of the Armed Forces of the Latin American region. During the analysis of both aspects, the key methodic role belongs to the comparative method. The quantitative parameters are represented in two aspects: national and international. The dynamics of the basic quantitative parameter, the Armed Forces number, and also the positioning of the armies of that region on the global scale are compared. Analysis. Since the eve of the 21st century, practically all the countries of Latin America have demonstrated a tendency toward the growth of the active military staff, proving the increment of the militarization of the region. That process represents one of the macrotendencies characterizing the actual development of the region. Results. According to the criteria of the militarization degree, three groups of countries are singled out: the stable high dynamics group, the middle dynamics indications group, and the low indications group. In the transformation of regional armies in the 21st century, the qualitative change is the appearance of a spectrum of new non-traditional functions. The hypotheses formulated in the study are the following: “The broadening of the sociopolitical functions of the military is related to the changes in attitudes toward security in the countries of the region.” The scrutiny of the military-industrial complexes of the Latin American countries gives a reason to affirm that their potentiality is mainly restricted in its character. The dependent and peripheric character of the region determines its quality as a market for the NATO countries military-technical arsenal.

VET centres: from a traditional to a transformational role for students, enterprises and society

Vocational Education and Training (VET) has the potential to move from a traditional function to a transformational ones by extending their activities beyond teaching provision. The transformational role of VET is focused on non-traditional functions like applied research and technical services provision to enterprises supporting SMEs innovation and local and regional development. However, these non-traditional functions belong to a complex process and depend both on internal and external factors to the centres being driven by the need for innovation and problem-solving of those enterprises outside the scope of the traditional institutions. Therefore, applied research in the VET field can be defined as solving real problems in industry that posits VET as a key agent in the Regional Innovation Systems. The approach to non-traditional functions, in general, and to applied research activities are considered some of the key characteristics of excellence in the VET field.

Bones and Hormones: Interaction between Hormones of the Hypothalamus, Pituitary, Adipose Tissue and Bone.

The bony skeleton, as a structural foundation for the human body, is essential in providing mechanical function and movement. The human skeleton is a highly specialized and dynamic organ that undergoes continuous remodeling as it adapts to the demands of its environment. Advances in research over the last decade have shone light on the various hormones that influence this process, modulating the metabolism and structural integrity of bone. More recently, novel and non-traditional functions of hypothalamic, pituitary, and adipose hormones and their effects on bone homeostasis have been proposed. This review highlights recent work on physiological bone remodeling and discusses our knowledge, as it currently stands, on the systemic interplay of factors regulating this interaction. In this review, we provide a summary of the literature on the relationship between bone physiology and hormones including kisspeptin, neuropeptide Y, follicle-stimulating hormone (FSH), prolactin (PRL), adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH), growth hormone (GH), leptin, and adiponectin. The discovery and understanding of this new functionality unveils an entirely new layer of physiologic circuitry.

PS-BPB07-6: NOVEL HUMORAL AND NEURONAL PATHWAYS FOR DIRECT BLOOD PRESSURE CONTROL BY MACULA DENSA CELLS

Objective: According to traditional physiological knowledge, macula densa (MD) cells of the juxtaglomerular apparatus (JGA) in the kidney maintain body fluid homeostasis and blood pressure (BP) indirectly by controlling renal and glomerular hemodynamics and renin secretion based on local sensory inputs. However, recently identified microanatomical (long, axon-like basal cell processes, secretory vesicles) and functional (high level of protein synthesis) features of MD cells suggest they perform non-traditional functions. The present study addressed the hypothesis that MD cells can directly and acutely control distant cardiovascular organ functions and blood pressure via novel secreted hormones and communications with renal nerves. Design and method: A comprehensive research approach used transgenic mouse models (MD-GFP, MD-Ai27 mice that selectively express the depolarizing channel rhodopsin ChR2 in MD cells), intravital multiphoton imaging, systemic BP monitoring via the cannulated carotid artery, whole mount kidney imaging and 3D analysis, and single-cell transcriptomic analysis. Results: Bulk and single cell MD transcriptome analysis identified the high expression of secreted angiogenic and vasoactive factors (Pappa2, Vash2, Cyr61, Aard, Pamr1) and synaptic proteins (Unc5d, Tenm2, Hpcal4, Nsg2, Syp, Syt5, Grin2c, Gabrr2, Begain) with their altered kidney and plasma levels in disease and MD gain and loss-of-function conditions. Immunohistochemistry and 3D tissue volume rendering of optically cleared whole-mount MD-GFP kidneys identified the high MD expression of synaptophysin and the close anatomical association between MD cell basal processes and tyrosin-hydroxylase (TH)+ or calcitonin gene-related peptide (CGRP)+ sympathetic and sensory nerve endings. Optogenetic MD stimulation was induced by blue light (470 nm) exposure of the whole left kidney of MD-Ai27 mice, which maneuver acutely and reversibly increased systemic BP by 22 ± 5mmHg (P < 0.05, n = 5). Conclusions: MD cells secrete hormones that target the vasculature and form synapses with sympathetic and sensory renal nerves. These novel MD and JGA pathways may be involved in direct BP control and represent exciting future therapeutic opportunities for renal and cardiovascular disease and hypertension.

Social and Psychological Analysis of the Transformation Family Functions in the Age of Globalization

In every society family is an institution created on the basis of the social laws, customs and traditions and is one of the main social inventions of humankind. Its main essence is defined by fulfilling social, group and individual needs. Family as one of the primary forms of social unity of humans provides conditions for meeting a range of important human needs, one of them being reproduction.Family functions are the main factors that precondition the existence and activity of family, as well as normal (functional) family environment. Fulfillment of these functions has exclusive importance not only for family members, individuals, but for the whole society. Researches on family psychology, sociology and pedagogy classify family functions on the basis of a variety of criteria. The paper analyzes traditional (specific) and non-traditional (non-specific) family functions from psychological perspective and classifies the social and psychological importance of changes in family functions in the age of globalization.

Evaluating the quality of research activities: Investigating into the uniqueness

Today, the research activities of scientists and research organizations is estimated through the informetrical methods (bibliometrics) with absolute and relative indicators based on the number of publications and their citations in scientometric databases. The leading authors and research organizations are given grants and awards, become international laureates, get financial support or other dividends. The authors argue that the method of research activities evaluation influences the authors’ behavior and increases plagiarism, rigged citation and academic dishonesty.The fraud practices misrepresent the estimations of research activities quality and the current status of a discipline and publication activities. The problem is analyzed; simultaneous use of bibliometric methods and most advanced scientific methods of modern methods of defining writing styles is suggested as a solution. The authors emphasize that traditionally libraries are meant to educate, to popularize knowledge and to inform. Today, they have to go into non-traditional functions, i.e. to support linguistic research and to build linguistic corpora. This would prevent manipulations when estimating publication activities with bibliometric methods and would promote academic honesty in academic practices.

Polycomb chromobox Cbx2 enhances antiviral innate immunity by promoting Jmjd3-mediated demethylation of H3K27 at the Ifnb promoter

Polycomb chromobox (CBX) proteins regulate gene transcription by maintaining chromatin states, which guide a variety of biological processes. Now, epigenetic regulation of innate immune response is an emerging field. However, the role of CBX proteins in innate immunity remains unclear. We confirmed that the expression of CBX family proteins, especially Cbx2, was decreased in macrophages upon viral infection, and then we investigated the role of Cbx2 in the antiviral immune response. Silencing or knockdown of Cbx2 in macrophages inhibited virus-induced production of IFN-β. Furthermore, heterozygous Cbx2 knockout were susceptible to VSV challenge. Mechanistically, Cbx2 binds to and recruits Jmjd3 to the Ifnb promoter, leading to demethylation of H3K27me3 and increased transcription of IFN-β. Together, our study reveals a non-traditional function of a Cbx protein and adds new insight into the epigenetic regulation of antiviral innate immunity.

Actions of pituitary hormones beyond traditional targets.

Studies over the past decade have challenged the long-held belief that pituitary hormones have singular functions in regulating specific target tissues, including master hormone secretion. Our discovery of the action of thyroid-stimulating hormone (TSH) on bone provided the first glimpse into the non-traditional functions of pituitary hormones. Here we discuss evolving experimental and clinical evidence that growth hormone (GH), follicle-stimulating hormone (FSH), adrenocorticotrophic hormone (ACTH), prolactin, oxytocin and arginine vasopressin (AVP) regulate bone and other target tissues, such as fat. Notably, genetic and pharmacologic FSH suppression increases bone mass and reduces body fat, laying the framework for targeting the FSH axis for treating obesity and osteoporosis simultaneously with a single agent. Certain 'pituitary' hormones, such as TSH and oxytocin, are also expressed in bone cells, providing local paracrine and autocrine networks for the regulation of bone mass. Overall, the continuing identification of new roles for pituitary hormones in biology provides an entirely new layer of physiologic circuitry, while unmasking new therapeutic targets.

Towards evidence of long-range correlations in shallow seismic activities

In this work, we introduce a new methodology to construct a network of epicenters that avoids problems found in well-established methodologies when they are applied to global catalogs of earthquakes located in shallow zones. The new methodology involves essentially the introduction of a time window which works as a temporal filter. Our approach is more generic and for small regions the results coincide with previous findings. The network constructed with that model has small-world properties and the distribution of node connectivity follows a non-traditional function, namely a q-exponential, where scale-free properties are present. The vertices with larger connectivity in the network correspond to the areas with very intense seismic activities in the period considered. These new results strengthen the hypothesis of long spatial and temporal correlations between earthquakes.

Beyond the Matrix: The Many Non-ECM Ligands for Integrins.

The traditional view of integrins portrays these highly conserved cell surface receptors as mediators of cellular attachment to the extracellular matrix (ECM), and to a lesser degree, as coordinators of leukocyte adhesion to the endothelium. These canonical activities are indispensable; however, there is also a wide variety of integrin functions mediated by non-ECM ligands that transcend the traditional roles of integrins. Some of these unorthodox roles involve cell-cell interactions and are engaged to support immune functions such as leukocyte transmigration, recognition of opsonization factors, and stimulation of neutrophil extracellular traps. Other cell-cell interactions mediated by integrins include hematopoietic stem cell and tumor cell homing to target tissues. Integrins also serve as cell-surface receptors for various growth factors, hormones, and small molecules. Interestingly, integrins have also been exploited by a wide variety of organisms including viruses and bacteria to support infectious activities such as cellular adhesion and/or cellular internalization. Additionally, the disruption of integrin function through the use of soluble integrin ligands is a common strategy adopted by several parasites in order to inhibit blood clotting during hematophagy, or by venomous snakes to kill prey. In this review, we strive to go beyond the matrix and summarize non-ECM ligands that interact with integrins in order to highlight these non-traditional functions of integrins.

Play It Again, SAM: Macrophages Control Peripheral Fat Metabolism

Macrophages and other immune cells are increasingly recognized to have unique and nontraditional functions in various tissues of the body. In a recent issue of Nature Medicine, Pirzgalska et al. [1] characterized a unique set of tissue-specialized macrophages that modulate the connection between the nervous system and subcutaneous fat.

African Swine Fever Virus NP868R Capping Enzyme Promotes Reovirus Rescue during Reverse Genetics by Promoting Reovirus Protein Expression, Virion Assembly, and RNA Incorporation into Infectious Virions.

Reoviruses, like many eukaryotic viruses, contain an inverted 7-methylguanosine (m7G) cap linked to the 5' nucleotide of mRNA. The traditional functions of capping are to promote mRNA stability, protein translation, and concealment from cellular proteins that recognize foreign RNA. To address the role of mRNA capping during reovirus replication, we assessed the benefits of adding the African swine fever virus NP868R capping enzyme during reovirus rescue. C3P3, a fusion protein containing T7 RNA polymerase and NP868R, was found to increase protein expression 5- to 10-fold compared to T7 RNA polymerase alone while enhancing reovirus rescue from the current reverse genetics system by 100-fold. Surprisingly, RNA stability was not increased by C3P3, suggesting a direct effect on protein translation. A time course analysis revealed that C3P3 increased protein synthesis within the first 2 days of a reverse genetics transfection. This analysis also revealed that C3P3 enhanced processing of outer capsid μ1 protein to μ1C, a previously described hallmark of reovirus assembly. Finally, to determine the rate of infectious-RNA incorporation into new virions, we developed a new recombinant reovirus S1 gene that expressed the fluorescent protein UnaG. Following transfection of cells with UnaG and infection with wild-type virus, passage of UnaG through progeny was significantly enhanced by C3P3. These data suggest that capping provides nontraditional functions to reovirus, such as promoting assembly and infectious-RNA incorporation.IMPORTANCE Our findings expand our understanding of how viruses utilize capping, suggesting that capping provides nontraditional functions to reovirus, such as promoting assembly and infectious-RNA incorporation, in addition to enhancing protein translation. Beyond providing mechanistic insight into reovirus replication, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP868R capping enzyme. Since reovirus shows promise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of recombinant reoviruses as candidates to enhance therapeutic potency. NP868R-assisted reovirus rescue will also expedite production of recombinant reovirus for mechanistic insights into reovirus protein function and structure.

A void and a challenge: Rules of engagement at sea in a low-intensity conflict environment

ABSTRACTThe armed forces are usually conversant with the rules on the use of force against the enemy in the traditional battle space. However, military forces are usually ill-equipped for this when the opponent is not a regular combatant but an un-uniformed person – a militant or even a criminal – operating in seemingly benign environs. This constitutes a serious legal void, particularly given the emerging environment wherein navies would be increasingly tasked to perform non-traditional constabulary functions. The trend is best exemplified by the case of the Indian Navy that was entrusted with the responsibility for the nation's overall maritime security, including coastal and offshore security. This paper examines the concept and the salient issues relating to rules of engagement (RoE) for maritime forces operating in the low-intensity conflict environment.

The multiple facets of alpha-1-antitrypsin.

Alpha-1-antitrypsin (AAT) is recognised as a potent inhibitor of serine proteinases. Genetic deficiency is associated with several neutrophilic diseases including severe emphysema. This is believed to reflect the loss of inhibition of neutrophilic serine proteinases that then result in local tissue damage (the proteinase/antiprotease hypothesis). In recent years the role of AAT in the control of inflammatory and immunological processes has become identified. Although in some instances this may reflect its ability to control pro-inflammatory effects of serine proteinases it has also become recognised that it has non proteolytic mediated functions. This poly functional role is starting to become recognised offering a possibility of its use as a therapeutic agent in many clinical disease areas. The current review explores both the traditional and non-traditional function of AAT through published literature.

Platelets: more than a sack of glue.

Platelets are primary effector cells in hemostasis. Emerging evidence over the last decade, however, demonstrates that platelets also have critical roles in immunity and inflammation. These nontraditional functions of platelets influence the development, progression, and evolution of numerous diseases, including arthritis, cancer, cardiovascular disease, and infectious syndromes. This chapters reviews recently discovered attributes of platelets that contribute to human disease, paying particular attention to the inflammatory activities of this anucleate cytoplast.

Once upon a storm: Katrina and nutrition support or the lack thereof.

Nutrition support teams face many challenges to establish, fund, maintain, and justify their existence. Some of the challenges can be resolved over time. However, the challenge of providing nutrition in general and nutrition support in specific during a natural disaster is void of the luxury of time experienced with the previously delineated challenges. The experience of Methodist Hospital in New Orleans, Louisiana, during Hurricane Katrina in 2005 in providing nutrition and nutrition support is summarized in this invited article. The recollections of various represented disciplines are bolstered by tables and figures that outline the 7 days before and after Katrina. Transdisciplinarity was exhibited through the performance of nontraditional functions or tasks by a variety of professionals who lived 5 days or longer in the “Methodist Island.” Lessons learned and considerations for disaster preparedness as it relates to nutrition and nutrition support as well as general considerations are provided.

An improved self-adaptive artificial bee colony algorithm for global optimisation

In this paper we propose an improved self-adaptive artificial bee colony algorithm (IS-ABC) for accurate numerical function optimisation. A modified self-adaptive mechanism based on 1/5th success rule is embedded in the structure of ABC to enhance the speed of the algorithm. The proposed algorithm has been tested on various numerical benchmark functions including the non-traditional functions proposed in CEC 2008 competition. To further validate performance of proposed algorithm we had also considered two challenging real world continuous optimisation problems. The results obtained with self-adaptive ABC have been compared with those obtained by new state-of-variants of PSO, DE, etc.

New paradigms in thrombosis: novel mediators and biomarkers platelet RNA transfer

Platelets, anucleated cells with a central role in hemostasis and inflammation, contain messenger RNAs and microRNAs of unknown functionality and clinical relevance. Historically, platelet RNA was viewed as merely a remnant of platelet biogenesis; however, several studies now refute this assumption. Studies have shown that platelets can actively translate RNA to protein and that specific RNA profiles correlate with select human clinical phenotypes. These studies support a more fluid role for platelet RNA in platelet function and disease development. Our lab and others have recently studied the platelet's unique ability to transfer RNA to recipient cells and the effect this transfer has on the recipient cells' functions. This transfer may represent a previously unknown form of vascular cell communication and modulation. Unlike the well-characterized thrombotic properties of platelets, the nature and purpose of platelet RNA transfer has not been determined, partly due to limitations in techniques used to manipulate platelet RNA profiles. Defining the mechanism of RNA transfer and its role in the vascular system will allow for the better understanding of how platelets function in both their traditional thrombotic role and non-traditional functions, potentially having widespread implications in several fields.

Oligodendrocytes in neurodegenerative diseases

Oligodendrocyte is a highly specialized glial cell type in the vertebrate central nervous system, which guarantees the long-distance transmission of action potential by producing myelin sheath wrapping adjacent axons. Disrupted myelin and oligodendrocytes are hallmarks of some devastating neurological diseases, such as multiple sclerosis, although their contribution to neurodegeneration in a given disease is still controversial. However, accumulating evidence from clinical studies and genetic animal models implicates oligodendrocyte dysfunction as one of major events in the processes of initiation and progression of neurodegeneration. In this article, we will review recent progress in understanding non-traditional function of oligodendrocytes in neuronal support and protection independent of myelin sheath and its possible contribution to neurodegeneration. Oligodendrocytes play a pivotal role in neurodegenerative diseases among which special emphasis is given to multiple system atrophy and Alzheimer’s disease in this review.